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101.
详细叙述从辣椒品种选择直至采摘的整个生长过程的栽培管理。栽培过程中,合理选择辣椒品种和栽培条件,进行科学的大田管理和病虫害防治是辣椒高产的关键技术。 相似文献
102.
L Cheng FL Booker C Tu KO Burkey L Zhou HD Shew TW Rufty S Hu 《Science (New York, N.Y.)》2012,337(6098):1084-1087
The extent to which terrestrial ecosystems can sequester carbon to mitigate climate change is a matter of debate. The stimulation of arbuscular mycorrhizal fungi (AMF) by elevated atmospheric carbon dioxide (CO(2)) has been assumed to be a major mechanism facilitating soil carbon sequestration by increasing carbon inputs to soil and by protecting organic carbon from decomposition via aggregation. We present evidence from four independent microcosm and field experiments demonstrating that CO(2) enhancement of AMF results in considerable soil carbon losses. Our findings challenge the assumption that AMF protect against degradation of organic carbon in soil and raise questions about the current prediction of terrestrial ecosystem carbon balance under future climate-change scenarios. 相似文献
103.
应用dsRNA测序技术检测草鱼呼肠孤病毒的混合感染 总被引:2,自引:1,他引:1
从草鱼(Ctenopharyngodon idellus)出血病疑似病料提取物感染的草鱼肾细胞(CIK)中提取病毒基因组dsRNA,电泳显示出水生呼肠孤病毒基因组的典型特征。应用简化的FLAC(full-length amplification of cDNA)技术扩增得到病毒的4个全长基因组片段进行测序分析。核酸序列BLAST分析表明:其中3个基因组片段与GCRV-873第5、9、10片段高度同源,另外1个基因组片段与GCRV-HZ08第11片段高度同源;因此推测病料中同时存在两种不同的病毒核酸,但也可能存在一种杂合病毒。根据已发表的GCRV-HZ08第5、9、10片段设计了3对引物对分离的病毒总RNA进行RT-PCR检测并测序,结果显示这3个片段与GCRV-HZ08第5、9、10片段均具有99%同源性,表明是由于混合感染而存在两株不同病毒的核酸dsRNA,两株病毒分别命名为GCRV-JX01和GCRV-JX02。首次运用dsRNA测序法检测出了GCRV病毒的混合感染,为草鱼呼肠孤病毒的流行病学和防控提供了一种新的技术选择。 相似文献
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Survey on the contamination of Toxoplasma gondii oocysts in the soil of public parks of Wuhan, China
Du F Feng HL Nie H Tu P Zhang QL Hu M Zhou YQ Zhao JL 《Veterinary parasitology》2012,184(2-4):141-146
Toxoplasma gondii of warm-blooded animals and humans is an important pathogenic agent throughout the world. Soil is increasingly recognized as an important source in the transmission of Toxoplasma. To attain the contamination status of T. gondii in the soil of public parks, a total of 252 soil samples were collected from September 2009 to August 2010 at different sites located in 6 public parks of Wuhan, Hubei, China and detected by PCR and loop-mediated isothermal amplification (LAMP). The detection limit of PCR/B1, PCR/529 and LAMP was determined to be 50, 5, and 5 tachyzoites in soil, respectively. Forty-one samples were found positive for Toxoplasma DNA by PCR on both genes, whereas LAMP products were generated in 58 samples (χ(2)=3.6328, P=0.0567). All parks were found contaminated and no significant difference was found among the parks (PCR: χ(2)=0.0072, P=0.9325; LAMP: χ(2)=0.6101, P=0.4347). However, contamination was found with significantly different among the four seasons (PCR: χ(2)=11.6066, P=0.0007; LAMP: χ(2)=12.4636, P=0.0004), with a gradual decrease in the prevalence from spring to winter on both analyses. This is the first investigation on soil contamination of public parks in China by T. gondii oocysts. The results indicate that the soil of public parks contaminated with T. gondii oocysts may play a role in the epidemiology of toxoplasmosis and effective preventive measures should be considered. Moreover, the conventional PCR and LAMP used in the present study are applicable to detect T. gondii oocysts in soil samples. 相似文献
106.
Zhiwen Liu Tingdong Fu Ying Wang Jinxing Tu Baoyuan Chen Yongming Zhou Chaozhi Ma Lianmin Shan 《Euphytica》2006,149(3):381-385
Summary We have previously identified 2 RAPD and 8 AFLP markers that were associated with yellow seed coat gene in a stable and pure yellow-seeded DH line No. 2127-17, however, they were not suitable in large-scale MAS. In this paper, it reported our efforts in developing rapid and reliable SCAR and CAPS markers from the RAPD and AFLP markers. Based on the sequence information in the regions of the most closely linked 4 AFLP and 2 RAPD markers, one SCAR (SCS1130) and one CAPS (SCA1) maker were successfully developed. Linkage analysis on a 127 DH lines population derived from the cross Hui5148-2 × No. 2127-17 revealed that they were tightly flanked (3.2 cM, 3.8 cM respectively). The resulting SCS1130 and SCA1 were further evaluated in backcross breeding families and demonstrated more accurate and valuable in MAS breeding. The development of these new markers would facilitate and accelerate the yellow seed coat breeding in Brassica napus. 相似文献
107.
[目的]研究内蒙古呼伦贝尔寸草苔草原群落特征及物种多样性与土壤理化性质的关系,为草原生态系统的保护和恢复提供科学参考。[方法]对呼伦贝尔寸草苔草原95个典型样地进行群落调查,研究呼伦贝尔寸草苔草原4种群落类型间群落特征及物种多样性与土壤理化性质之间的关系。[结果](1)群落特征中,地上生物量、物种丰富度和Shannon-Wiener指数在4种群落类型间以寸草苔—一二年生草类群落最高;土壤理化性质中,全氮和全磷均以寸草苔—根茎型草类群落最高。(2)寸草苔—丛生草类群落和寸草苔—杂类草群落中,土壤有机碳、全氮、全磷与地上生物量、地下生物量呈显著正相关关系。(3)寸草苔—根茎型草类群落和寸草苔—一二年生草类群落中,土壤有机碳、全氮、全磷与Shannon-Wiener指数、Simpson指数、Pielou指数呈显著负相关关系。[结论]呼伦贝尔寸草苔草原群落特征在4种群落类型间存在不同程度差异,土壤理化性质中土壤有机碳、全氮和全磷是影响群落特征和物种多样性差异的主要因子。 相似文献
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The recent research achievements about the mechanisms of beneficial health effects of tea polyphenols and the epidemiological investigation on anti-cancer,anti-cardiovascular diseases,and anti-obesity through tea consumption were reviewed.It is proved that reactive oxygen species is the main reason to induce cell mutation,DNA damage,obesity,high blood pressure and many kinds of cancers.Tea catechins and theaflavins have excellent bioactivity on scavenging ROS due to their characteristic structure feature of phenolic hydroxyl group,which means drinking tea could prevent diseases.Epidemidogical investigations also showed that tea consumption could reduce the risk of lung cancer,gastric cancer,bladder cancer,and so on.However,the frequency of tea drinking or tea consumption amounts significantly affected the prevention efficiency.The epidemiologic investigations also find that drink green tea,especial black tea is useful to modify the cardiovascular diseases.In vivo and vitro tests showed that tea supplement could moderate the metabolism of blood lipid and prevent obesity.These indicated that tea and tea extracts might reduce the risk of many diseases. 相似文献