Development of SCAR and CAPS Markers for a Partially Dominant Yellow Seed Coat Gene in Brassica Napus L

Summary We have previously identified 2 RAPD and 8 AFLP markers that were associated with yellow seed coat gene in a stable and pure yellow-seeded DH line No. 2127-17, however, they were not suitable in large-scale MAS. In this paper, it reported our efforts in developing rapid and reliable SCAR and CAPS markers from the RAPD and AFLP markers. Based on the sequence information in the regions of the most closely linked 4 AFLP and 2 RAPD markers, one SCAR (SCS1130) and one CAPS (SCA1) maker were successfully developed. Linkage analysis on a 127 DH lines population derived from the cross Hui5148-2 × No. 2127-17 revealed that they were tightly flanked (3.2 cM, 3.8 cM respectively). The resulting SCS1130 and SCA1 were further evaluated in backcross breeding families and demonstrated more accurate and valuable in MAS breeding. The development of these new markers would facilitate and accelerate the yellow seed coat breeding in Brassica napus.     

呼伦贝尔寸草苔草原群落特征及物种多样性与土壤理化性质的关系

[目的]研究内蒙古呼伦贝尔寸草苔草原群落特征及物种多样性与土壤理化性质的关系,为草原生态系统的保护和恢复提供科学参考。[方法]对呼伦贝尔寸草苔草原95个典型样地进行群落调查,研究呼伦贝尔寸草苔草原4种群落类型间群落特征及物种多样性与土壤理化性质之间的关系。[结果](1)群落特征中,地上生物量、物种丰富度和Shannon-Wiener指数在4种群落类型间以寸草苔—一二年生草类群落最高;土壤理化性质中,全氮和全磷均以寸草苔—根茎型草类群落最高。(2)寸草苔—丛生草类群落和寸草苔—杂类草群落中,土壤有机碳、全氮、全磷与地上生物量、地下生物量呈显著正相关关系。(3)寸草苔—根茎型草类群落和寸草苔—一二年生草类群落中,土壤有机碳、全氮、全磷与Shannon-Wiener指数、Simpson指数、Pielou指数呈显著负相关关系。[结论]呼伦贝尔寸草苔草原群落特征在4种群落类型间存在不同程度差异,土壤理化性质中土壤有机碳、全氮和全磷是影响群落特征和物种多样性差异的主要因子。     

大豆磷脂油对河田鸡脂肪代谢与肉质性状的影响

研究了大豆磷脂对河田鸡生产性能、脂肪代谢和肉质性状的影响。结果表明，在日粮中添加大豆磷脂，可改善生产性能，降低料肉比，提高肌内脂肪含量，而降低腹脂率。在日粮中添加大豆磷脂可以显著降低肝脏苹果酸酶(ME)活性以及降低肌肉的滴水损失、烹饪损失、剪切力。     

提高大树进城移栽成活的关键技术——以峡江县城区绿化建设为例

主要分析影响大树移栽成活率因素,从起苗、包装、栽植、树体管理、施肥、修剪等环节提出具体的技术措施,并详述大树移植技术要点和栽植后树体养护管理措施,有利于提高大树移植成活率。     

Benefits of Daily Intake Tea on Human Health

The recent research achievements about the mechanisms of beneficial health effects of tea polyphenols and the epidemiological investigation on anti-cancer,anti-cardiovascular diseases,and anti-obesity through tea consumption were reviewed.It is proved that reactive oxygen species is the main reason to induce cell mutation,DNA damage,obesity,high blood pressure and many kinds of cancers.Tea catechins and theaflavins have excellent bioactivity on scavenging ROS due to their characteristic structure feature of phenolic hydroxyl group,which means drinking tea could prevent diseases.Epidemidogical investigations also showed that tea consumption could reduce the risk of lung cancer,gastric cancer,bladder cancer,and so on.However,the frequency of tea drinking or tea consumption amounts significantly affected the prevention efficiency.The epidemiologic investigations also find that drink green tea,especial black tea is useful to modify the cardiovascular diseases.In vivo and vitro tests showed that tea supplement could moderate the metabolism of blood lipid and prevent obesity.These indicated that tea and tea extracts might reduce the risk of many diseases.     

丰产优质转基因抗虫棉杂交种——赣棉207

江西省棉花研究所/鄱阳湖生态育种站,江西 九江 322105     

多尺度空间信息服务技术及其农业应用

从省市县三级空间尺度和应用特征的区域信息服务出发,研究基于网络的多源多尺度农业空间信息共享与服务技术.集成应用空间信息技术与Web服务技术,开展多源多尺度的农业空间信息资源的数据整合、统一业务模型构造、信息交换与共享、元数据目录服务等关键技术研究,建成多尺度的基础信息数据库群,开发实现了省级区域多源多尺度应用的空间信息服务平台.有效整合多源多尺度的农业信息资源,促进了区域空间信息应用与服务.     

超高压微射流对花生蛋白结构的影响（简报）

为了探讨超高压微射流对花生蛋白理化性质和结构的影响,该文研究了花生蛋白溶液经超高压微射流处理后的颗粒大小、游离巯基基团、疏水基团和紫外吸收基团的变化规律。结果表明：花生蛋白的颗粒尺寸和游离巯基基团含量随着超高压微射流均质压力的增大而显著减小;疏水基团和紫外吸收基团的含量则随着均质压力的增大而显著增大,说明超高压微射流处理可破坏花生蛋白的内部基团,使蛋白的结构发生变化。     

A New PCR/LDR-Based Multiplex Functional Molecular Marker for Marker-Assisted Breeding in Rice

Marker-assisted selection or marker-aided selection(MAS)provides an effective complementary approach for conventional rice breeding with precise and speedy mobilization of target genes into elite genetic backgrounds.The targeted genes,however,may not be selected in the course of MAS due to the occasional recombination between the marker and the target gene/QTL during the many cycles of meiosis involved in breeding programs.This leads to failure in the selection of target traits(Gopalakrishnan et al,2008).     

2013—2016年上海市项目核心示范猪场主要疫病的免疫抗体监测

为评估上海市部分规模猪场的免疫抗体水平,更好地指导生产实践,对9个项目核心示范猪场2013—2016年间1 606份血清样品进行口蹄疫、猪瘟和高致病性猪蓝耳病等3种主要疫病的免疫抗体检测,并对不同规模场、不同区域、不同年份、不同采样时间点和不同阶段猪群的免疫抗体水平进行比较分析。结果显示：2013—2016年上海市部分规模猪场O型口蹄疫免疫抗体平均合格率为85.13%（95% CI：83.39%~86.87%）,猪瘟免疫抗体平均合格率为84.65%（95% CI：82.89%~86.41%）,高致病性猪蓝耳病免疫抗体平均合格率为95.77%（95% CI：94.79%~96.75%）;不同地区、不同年份间的部分疫病抗体合格率存在差异;9个核心示范猪场3种主要疫病免疫抗体水平呈现逐年上升趋势;不同采样时间点的O型口蹄疫免疫抗体合格率差异极显著,其他疫病不显著;生产母猪的免疫抗体水平优于商品肉猪。检测结果显示,通过持续跟踪监测、不断优化免疫程序、调整疫苗种类,上海市规模猪场的主要疫病得到了良好的控制。