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41.
S. Noguchi T. Mori Y. Hoshino N. Yamada K. Maruo Y. Akao 《Veterinary and comparative oncology》2013,11(2):113-123
Malignant melanoma (MM) is one of the most aggressive cancers in dogs and in humans. However, the molecular mechanisms of its development and progression remain unclear. Presently, we examined the expression profile of microRNAs (miRs) in canine oral MM tissues and paired normal oral mucosa tissues by using the microRNA‐microarray assay and quantitative RT‐PCR. Importantly, a decreased expression of miR‐203 was significantly associated with a shorter survival time. Also, miR‐203 and ‐205 were markedly down‐regulated in canine and human MM cell lines tested. Furthermore, the ectopic expression of miR‐205 had a significant inhibitory effect on the cell growth of canine and human melanoma cells tested by targeting erbb3. Our data suggest that miR‐203 is a new prognostic factor in canine oral MMs and that miR‐205 functions as a tumour suppressor by targeting erbb3 in both canine and human MM cells. 相似文献
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43.
Longsheng Fu Hiroshi Okamoto Yoichiro Hoshino Takashi Kataok Youichi Shibata 《农业工程》2012,2(2):119-121
高灌蓝莓的成熟期不一致,在3~4周时间内需要有选择地采收3~5次。现在日本的蓝莓收获方式是采用一种具有5个速度调节挡的18mm线锯对树枝进行变速振动。为了能够找到采收效率最高且采收效果最好的振动速度,利用高速摄像机对蓝莓采收过程进行记录。之后利用数据统计,回放分析采收过程中的细节,以确定最佳振动速度和振动时间。通过对5种速度的分析,发现Ⅱ挡即频率为1670次min,在果枝周围振动2s是采收的最佳方式。在这种条件下,与3个高速挡同样可以分离87%成熟的蓝莓。同时仅分离了24%未成熟的蓝莓,该结果与Ⅰ挡无显著差异。 相似文献
44.
Tamotsu Hoshino Anne Marte Tronsmo Naoyuki Matsumoto Tomomi Sakamoto Satoru Ohgiya Kozo Ishizaki 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(4):357-361
An extracellular lipolytic enzyme active at low temperature was purified from the culture filtrate of the snow mold fungus, Typhula ishikariensis group III, strain 6-1-1. The molecular mass of enzyme was approximately 83 kDa (SDS-PAGE). The lipolytic enzyme was most active for p-nitrophenyl palmitate at 30 –C (pH 9.0). The lipolytic activity was 23.4% of the maximum at 4 –C, the temperature of culture. Thus, the isolated T. ishikariensis lipolytic enzyme is thought to represent a new member of a group of enzymes active at low temperature. 相似文献
45.
Yasuharu Hoshino Hitoshi Yonenobu Koh Yasue Yoshihiro Nobori Takumi Mitsutani 《Journal of Wood Science》2008,54(3):183-188
We developed ring-width chronologies for living trees of Japanese beech at two forest sites on the northern-most part of Honshu
Island, Japan. A statistical threshold (running expressed population signal) yielded these site chronologies spanning 1853–1994
(142 years) and 1867–1994 (128 years). We examined two factors, climate and masting, that could affect the variations of radial
growth. The response function analysis revealed that the ring width correlated positively with July and August temperatures
of the previous growth year. The optimal radial growth of Japanese beech may largely depend on a warm previous summer with
above-average temperatures. The years of good masting coincided mostly with those showing abrupt growth depression, although
only the short-term records of masting were available.
Part of this work was presented at the 6th Pacific Regional Wood Anatomy Conference, Kyoto, December 2005, and the 7th International
Conference on Dendrochronology, Beijing, June 2006 相似文献
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47.
Akira Hoshino Masao Tsuji Kazuhiko Fukuda Miki Nonagase Hideo Sawada Makoto Kimura 《Soil Science and Plant Nutrition》2013,59(4):469-473
Among the biodegradable plastic specimens (poly-(3-hydroxy-butylate-valerate) (PHB / V), poly-(ε-caprolactone) (PCL), poly-(butylene succinate) (PBS), poly-(butylene succinate and adipate) (PBSA), and poly-lactide (PLA)) that were placed in soils for 1 year at nineteen sites in Japan, plastic specimens with appreciable biodegradation were studied for the transformation of the chemical structure by FT-IR, 1H-NMR, and 13C-NMR. No appreciable differences in the main absorbency-bands of the atomic groups were recognized by FT-IR for any of the plastic specimens tested. However, both 1H-NMR and 13C-NMR analyses suggested that molecular structure of the PHB / V specimens changed after 1 year placement in soils. Based on the assignment of the respective signals of chemical shifts derived from valerate, selective degradation of the valerate moiety in the PHB / V specimens was observed. In contrast, although weight loss, and/or a decrease in tensile strength and elongation were observed after the placement in soils for the PCL, PBS, PBSA, and PLA specimens, the analyses of these specimens by FT-IR, 1H-NMR, and 13C-NMR did not reveal any changes in their molecular structure. 相似文献
48.
Hiroko Akiyama Sho Morimoto Kanako Tago Yuko T. Hoshino Kazunari Nagaoka Masatsugu Yamasaki 《Soil Science and Plant Nutrition》2013,59(4):520-529
Nitrous oxide (N2O) is a greenhouse gas that contributes to the destruction of stratospheric ozone, and agricultural soil is an important source of N2O. Aerobic soils are sinks for atmospheric methane (CH4), a greenhouse gas. Ammonia monooxygenase (AMO) can oxidize CH4, but CH4 is mostly oxidized by methane monooxygenase (MMO), and CH4 oxidation by AMO is generally negligible in the soil. We monitored the N2O and CH4 fluxes after urea application in fields containing different soils using an automated sampling system to determine the effects of environmental and microbial factors on the N2O and CH4 fluxes. The soil types were Low-humic Andosol (Gleyic Haplic Andosol), yellow soil (Gleyic Haplic Alisol) and gray lowland soil (Entric Fluvisol). Cumulative N2O emissions from the yellow soil were higher than those from other soil types, although the difference was not significant. The CH4 uptake level by Andosol was one order of magnitude higher than that by other soils. There were significant relationships between the ammonia oxidation potential, AOB and AOA amoA copy numbers, and the CH4 uptake. In contrast, the gene copy numbers of methane-oxidizing bacteria (MOB) pmoA were below the detection limit. Our results suggested that the AMOs of AOB and AOA may have more important roles than those previously considered during CH4 oxidation in agricultural soils treated with N fertilizers. 相似文献
49.
Summary Wheat breeding efforts for a half century in Japan were investigated by using of 129 varieties registered in MAFF from 1929 to 1984 in the view point of case history for three years. Varieties released in each breeding station are classified apparently by growth habit associated closely with duration of the cold requirement. Heading date is earlier in varieties released in southwest breeding stations (southern varieties) than in varieties released in northern breeding stations (northern varieties). Culm length is higher and the pre-harvest sprouting is more sensitive in northern varieties. In quality characters, northern varieties has the higher milling rate, lower apparent amylose content and larger particle size of flour. In varieties released in the Tohoku district, trend of breeding direction with time is smaller ear numbers and larger 1,000-grain weight and greater resistance to powdery mildew. In varieties released in the Kyushu district, a significant correlation with released year was clearly observed with regard to early maturity, short culm length, less grain crude protein content, less apparent amylose content and higher milling rate on breeding advancement. 相似文献
50.
Michinari Matsushita Daisuke Hoshino Shin-Ichi Yamamoto Naoyuki Nishimura 《Journal of Forest Research》2014,19(1):134-142
Structures and dynamics of old-growth coniferous stands are affected by several types of disturbances including typhoons. We report the forest dynamics of four old-growth Chamaecyparis stands in central Japan that differ in the disturbance history of typhoons over a period of 23 years. The stem number, basal area and mortality were examined. In a predominant stand of C. obtusa (Sieb. et Zucc.) Endl., 24 % of the C. obtusa canopy trees died, mainly as a result of the severe damage of a strong typhoon that caused a single tree-fall gap and the following gap enlargements. In this stand, the total basal area decreased to 76.5 % of the initial value, although the mortality declined in recent years. In contrast, the other three stands decreased only slightly in the stem numbers (0.0–5.6 %) and increased in the basal areas of C. obtusa canopy trees. It is confirmed that the stand-level ingrowths of 300-year-old C. obtusa canopy trees could contribute to the increase in the stock of each stand. Our results support an idea that the dynamics of old-growth Chamaecyparis forests were greatly affected by typhoons. The stand structures will be gradually changed (with the processes of gap dynamics) and C. obtusa will continue to be dominant, potentially over hundreds of years. 相似文献