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Tomoaki Tochitani Izumi Matsumoto Kohei Hoshino Kaoru Toyosawa Mami Kouchi Takatoshi Koujitani Juki Kimura Hitoshi Funabashi 《Journal of toxicologic pathology》2013,26(2):187-191
The common marmoset (Callithrix
jacchus) is now widely used in various
research fields, including toxicology. However,
information about the background pathology of this
species is scarce. Here, we report a case of
rhabdomyosarcoma that spontaneously occurred in a
common marmoset. A 44-month-old male common
marmoset was euthanized due to bilateral hind limb
paralysis. At necropsy, a 2×2×5-cm intramuscular
mass was observed in the lower right back.
Histologically, the mass was mainly composed of
interlacing bundles of spindle-shaped tumor cells.
Immunohistochemically, the tumor cells were
positive for myogenin, desmin, vimentin and
alpha-smooth muscle actin. Ultrastructurally, the
tumor cells contained bundles of myofilaments with
Z-band-like structures. Thus, the tumor was
diagnosed as a rhabdomyosarcoma. To our knowledge,
this is the first report of spontaneous
rhabdomyosarcoma that was definitely diagnosed in
the common marmoset. 相似文献
34.
Sachiko Ikeda Tamotsu Hoshino Naoyuki Matsumoto Norio Kondo 《Journal of General Plant Pathology》2017,83(1):46-50
Typhula winter rot on overwintering carrots caused by Typhula variabilis is a newly confirmed disease, and no practical control measure is yet available. To develop a control method, here we researched the infection period of T. variabilis and the time that winter rot appeared on carrots. Using spore traps, we found that basidiospore rain occurred from September to November before snowfall in Memuro, Hokkaido. In addition, carrot leaves collected in autumn had already been infected by T. variabilis. These epidemiological investigations revealed that the pathogen releases basidiospores to infect carrot leaves before snow cover, resulting in root decay under snow. An effective control method was then developed to avoid direct contact of basidiospores of T. variabilis with plant tops by covering the plants with soil in autumn. Thus, the percentage of rotted roots was reduced to about half. 相似文献
35.
Tanaka A Hoshinoo K Hoshino T Tagawa Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(3):255-262
Nucleotide sequences of 16S rDNA and rpoB gene of 25 bovine and 6 ovine Histophilus somni strains were determined to detect subtle differences between the host animal species. The 1465 nucleotide residues of the 16S rDNA exhibited levels of sequence similarities of 99.4% or more. The high sequence similarity of the 16S rDNA of recently described species H. somni was confirmed in the 31 strains from cattle and sheep. These results suggested that the intra-specific diversity of 16S rDNA was limited in bovine and ovine strains of H. somni. The specific association of strains was also observed in the 311 bp region of rpoB gene which sequence similarities were 98.6% or more. However, the phylogenetic tree analysis of the rpoB gene showed that the ovine strains appeared to form a subgroup recovered in 70% of the bootstrap trees. In the 311 bp region of the ovine strains, a HincII restriction endonuclease site was detected. The PCR-amplified rpoB DNA of 46 bovine and 20 ovine H. somni strains were examined for the digestion with HincII. As the results, 17 strains of ovine strains were cleaved by the enzyme but none of the bovine strains appeared to possess the restriction site. The restriction enzyme analysis of rpoB gene may be useful to differentiate ovine strains from bovine strains of H. somni. 相似文献
36.
N Nose Y Hoshino Y Kikuchi M Horie K Saitoh T Kawachi H Nakazawa 《Journal of the Association of Official Analytical Chemists》1987,70(4):714-717
A liquid chromatographic method is described to determine simultaneously the following 11 synthetic antibacterial agents used in a fishery: nitrofuran derivatives furazolidone, nifurpirinol, difurazone, and furamizole; sulfa drugs sulfamerazine, sulfisozole, sulfamonomethoxine, and sulfadimethoxine; and, oxolinic, nalidixic, and piromidic acids. A Nucleosil C18 column was used with tetrahydrofuran-acetonitrile-phosphoric acid-water (29 + 1 + 0.06 + 69.94) as the mobile phase. Pretreatment of the fish meat sample with acetone extraction and alumina column cleanup gave good separation of the LC peaks without interference from any other components. Recovery of the antibacterial agents was ca 80%. The lower limit of detection of the drugs was 1-2 ng for 10 microL injection. 相似文献
37.
Watanabe E Hoshino R Kanzaki Y Tokumoto H Kubo H Nakazawa H 《Journal of agricultural and food chemistry》2002,50(13):3637-3646
The present work describes the design and synthesis of the structurally unique hapten, "bifunctional hapten", to produce a group-specific polyclonal antiserum to triclopyr and 3,5,6-trichloro-2-pyridinol. A bifunctional hapten was designed and synthesized by conjugating commercially available Nepsilon-2,4-dinitrophenyl (DNP)-L-lysine to triclopyr, and then coupling this to carrier proteins such as bovine serum albumin (BSA). The synthesized bifunctional hapten greatly raised the antiserum titer in comparison with that of the conventional hapten, triclopyr. Antiserum with a sufficiently high titer to provide the determinations of targeted compounds was obtained only 63 days after the primary immunization. The obtained antiserum showed the highest affinity to triclopyr (IC(50) = 3.5 nM) and 3,5,6-trichloro-2-pyridinol (IC(50) = 5.1 nM) in homologous ELISA. The cross-reactivities to various agrochemicals and some chlorinated phenolic compounds were determined. Significant cross-reactivity was found to the herbicide 2,4,5-T. The antiserum reacted to both triclopyr and its metabolite. Assay sensitivity was evaluated for effects of various assay conditions, including pH value and concentrations of organic solvents and detergents. Under optimized assay conditions, the quantitative working range of triclopyr ELISA was from 0.1 to 5.2 ng/mL with a limit of detection (LOD) of 0.037 ng/mL, and an IC(50) of 0.72 ng/mL. On the other hand, the quantitative working range of 3,5,6-trichloro-2-pyridinol ELISA was from 0.13 to 6.0 ng/mL with a LOD of 0.052 ng/mL, and an IC(50) of 0.95 ng/mL. Water samples fortified with triclopyr or its metabolite at 1, 5, and 10 ng/mL were directly analyzed without extraction and cleanup by the proposed ELISA. The mean recovery was 101.6%, and the mean coefficient of variation (CV) was 7.1% in the case of the triclopyr ELISA. In the case of the 3,5,6-trichloro-2-pyridinol ELISA, the mean recovery was 99.8%, and the mean CV was 9.5%. The proposed ELISA turned out to be a powerful tool for monitoring of residual triclopyr or 3,5,6-trichloro-2-pyridinol in water samples at trace level. 相似文献
38.
Kubota Kenji Tsukahara Narutoshi Hoshino Keisuke Ikeda Kentaro Sakai Hiroshi Toriyama Kimihiko 《Journal of General Plant Pathology》2022,88(5):300-308
Journal of General Plant Pathology - Variable domain of heavy chain of heavy-chain (VHH) antibodies (Abs) have been widely applied for the detection and therapeutics of viral pathogens. Here, we... 相似文献
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Takako Yazaki Yuki Hiradate Yumi Hoshino Kentaro Tanemura Eimei Sato 《Animal Science Journal》2013,84(5):395-402
We investigated the effect of oxidative stress induced by hydrogen peroxide (H2O2) on lipid peroxide (LPO) level and nuclear maturation in porcine oocytes cultured with or without cumulus cells. After 22 h of pre‐culture, oocytes with attached cumulus cells (COC group) or denuded oocytes (DO group) were cultured with H2O2, and intra‐oocyte H2O2 and LPO levels were quantitatively analyzed using immunofluorescence. This is the first report evaluating LPO levels in porcine oocytes. After H2O2 supplementation, the DO group showed severe accumulation of H2O2 and LPO in the oocytes. Similarly, while inhibition of progression of nuclear maturation was observed in both groups, the effect was more severe in the DO group. These results demonstrate that cumulus cells reduce the accumulation of H2O2 stress in oocytes. Furthermore, we attempted to reduce the oxidative stress by H2O2 with L‐carnitine, a H2O2 scavenger. L‐carnitine decreased H2O2 and LPO levels in the oocytes in both groups, and improvement in the progression of impaired nuclear maturation was observed. These effects were different by the presence of cumulus cells. Our results provide that L‐carnitine is useful for alleviating H2O2‐induced oxidative stress by reducing LPO levels and improving the progression of nuclear maturation. 相似文献