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排序方式: 共有227条查询结果,搜索用时 31 毫秒
61.
K Miyahara S Tose H Sakurai I Igarashi A Saito T Hirose N Suzuki 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(4):629-635
In order to identify mechanisms responsible for the anti-tumor effects of Toxoplasma lysate antigen (TLA), we used an in vitro 51Cr release assay to study the functional properties of plastic-adherent cells during induction of splenic cytotoxic activity by TLA. Cytotoxic activity of non-adherent cells was measured in all experiments after a 6 days incubation. Induction of cytotoxic non-adherent cells by TLA required the presence of plastic-adherent spleen cells. In contrast, rhIL-2 alone was able to induce transformation of cytotoxic non-adherent cells from non-adherent spleen cells. Contact between adherent and non-adherent spleen cells was necessary for successful induction of cytotoxic non-adherent cells by TLA. Treatment of spleen cells with anti-macrophage serum prevented induction of cytotoxic activity by TLA. Biologically active IL-2 was not detected in culture supernatants of spleen cells exposed to TLA. These findings suggest that contact between TLA-sensitized non-adherent cells and macrophages is necessary for induction of cytotoxic cells in the presence of TLA. This contact, however, is not necessary for generation of IL-2-induced killer cells. 相似文献
62.
L H Makala Y Nishikawa T Kamada H Suzuki X Xuan I Igarashi H Nagasaw 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(12):1271-1277
A detailed comparison of the accessory cell activities was carried out among murine peritoneal cavity macrophages (PEC-Mphi), peritonea] cavity macrophages stimulated with granulocyte-macrophage colony stimulating factor (GM-CSF) plus interleukin 4 (IL-4), the most popular cytokine combination widely used to generate dendritic cells (DC) and peritoneal cavity macrophage-derived DC (PEC-DC) using a two-way mixed lymphocyte reaction (MLR). All the cell types used efficiently induced statistically significant na?ve T cell proliferation at all culture time points and responder:stimulator ratios used. However, marked differences were noted in the magnitude of the proliferative responses. These variations may be attributed to the intensity of expression of MHC class II glycoproteins, as well as the actual numbers of MHC class II+ cells. 相似文献
63.
Igarashi I Asaba U Xuan X Omata Y Saito A Nagasawa H Fujisaki K Suzuki N Iwakura Y Mikami T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(7):717-723
The surface proteins of Babesia rodhaini have previously been shown to induce a high degree of protective immunity. In the present study, one of those proteins, B. rodhaini antigen p26 was expressed in Escherichia coli and in insect cells infected with a recombinant baculovirus. These proteins were recognized by immune serum from a drug-cured BALB/c mouse. While BALB/c mice immunized with both recombinant antigens and Freund's adjuvants showed 40-100% survival rate against challenge infection with B. rodhaini, saponin failed to induce protection, although significant levels of B. rodhaini-specific antibodies were produced in both immunized mice (1:1,000-2,000 by indirect immunofluorescent antibody test). The immunization of IFN-gamma-deficient mice with the recombinant proteins was not protective against B. rodhaini infection, indicating that IFN-gamma is one of the important factors for the survival against lethal B. rodhaini infection. 相似文献
64.
65.
Subedi R Shneor R Monaghan P Anderson BD Aniol K Annand J Arrington J Benaoum H Benmokhtar F Boeglin W Chen JP Choi S Cisbani E Craver B Frullani S Garibaldi F Gilad S Gilman R Glamazdin O Hansen JO Higinbotham DW Holmstrom T Ibrahim H Igarashi R de Jager CW Jans E Jiang X Kaufman LJ Kelleher A Kolarkar A Kumbartzki G Lerose JJ Lindgren R Liyanage N Margaziotis DJ Markowitz P Marrone S Mazouz M Meekins D Michaels R Moffit B Perdrisat CF Piasetzky E Potokar M Punjabi V Qiang Y Reinhold J Ron G 《Science (New York, N.Y.)》2008,320(5882):1476-1478
The protons and neutrons in a nucleus can form strongly correlated nucleon pairs. Scattering experiments, in which a proton is knocked out of the nucleus with high-momentum transfer and high missing momentum, show that in carbon-12 the neutron-proton pairs are nearly 20 times as prevalent as proton-proton pairs and, by inference, neutron-neutron pairs. This difference between the types of pairs is due to the nature of the strong force and has implications for understanding cold dense nuclear systems such as neutron stars. 相似文献
66.
Ishii N Nakahigashi K Baba T Robert M Soga T Kanai A Hirasawa T Naba M Hirai K Hoque A Ho PY Kakazu Y Sugawara K Igarashi S Harada S Masuda T Sugiyama N Togashi T Hasegawa M Takai Y Yugi K Arakawa K Iwata N Toya Y Nakayama Y Nishioka T Shimizu K Mori H Tomita M 《Science (New York, N.Y.)》2007,316(5824):593-597
Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations. 相似文献
67.
Nakamura T Noguchi T Tanaka M Zolensky ME Kimura M Tsuchiyama A Nakato A Ogami T Ishida H Uesugi M Yada T Shirai K Fujimura A Okazaki R Sandford SA Ishibashi Y Abe M Okada T Ueno M Mukai T Yoshikawa M Kawaguchi J 《Science (New York, N.Y.)》2011,333(6046):1113-1116
The Hayabusa spacecraft successfully recovered dust particles from the surface of near-Earth asteroid 25143 Itokawa. Synchrotron-radiation x-ray diffraction and transmission and scanning electron microscope analyses indicate that the mineralogy and mineral chemistry of the Itokawa dust particles are identical to those of thermally metamorphosed LL chondrites, consistent with spectroscopic observations made from Earth and by the Hayabusa spacecraft. Our results directly demonstrate that ordinary chondrites, the most abundant meteorites found on Earth, come from S-type asteroids. Mineral chemistry indicates that the majority of regolith surface particles suffered long-term thermal annealing and subsequent impact shock, suggesting that Itokawa is an asteroid made of reassembled pieces of the interior portions of a once larger asteroid. 相似文献
68.
Ground-water radon anomaly before the kobe earthquake in Japan 总被引:1,自引:0,他引:1
Igarashi G Saeki S Takahata N Sumikawa K Tasaka S Sasaki Y Takahashi M Sano Y 《Science (New York, N.Y.)》1995,269(5220):60-61
Radon concentration in ground water increased for several months before the 1995 southern Hyogo Prefecture (Kobe) earthquake on 17 January 1995. From late October 1994, the beginning of the observation, to the end of December 1994, radon concentration increased about fourfold. On 8 January, 9 days before the earthquake, the radon concentration reached a peak of more than 10 times that at the beginning of the observation, before starting to decrease. These radon changes are likely to be precursory phenomena of the disastrous earthquake. 相似文献
69.
Sayaka KITAUCHI Mizuki MAEDA Tetsushi HIRANO Yoshinori IKENAKA Misaki NISHI Asuka SHODA Midori MURATA Youhei MANTANI Toshifumi YOKOYAMA Yoshiaki TABUCHI Nobuhiko HOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):746
Recently, developmental exposure to clothianidin (CLO) has been shown to cause reproductive toxicity in male mice, but the effects in female mice remain to be clarified. Pregnant C57BL/6N mice were given a no-observed-adverse-effect-level (NOAEL) dose of CLO until weaning. We then examined ovaries of 3- or 10-week-old female offspring. In the CLO-administered group, morphological changes, a decrease in the immunoreactivity of the antioxidant enzyme glutathione peroxidase 4 (GPx4), and activation of genes in the steroid hormone biosynthesis pathway were observed in 3-week-old mice, and decreases of GPx4 immunoreactivity, 17OH-progesterone and corticosterone levels were observed in 10-week-old mice, along with high rates of infanticide and severe neglect, providing new evidence that developmental exposure to CLO affects juvenile and adult mice differently. 相似文献
70.