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Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method 下载免费PDF全文
Tao Zhuang Megumi Urakawa Hidetoshi Sato Yuko Sato Teruaki Taguchi Tsuyoshi Umino Shiro Katto Koutaro Tanaka Kozue Yoshimura Naokazu Takada Hiroko Kobayashi Megumi Ito Michael T. Rose Yoshio Kiku Yuya Nagasawa Haruki Kitazawa Kouichi Watanabe Tomonori Nochi Tomohito Hayashi Hisashi Aso 《Animal Science Journal》2018,89(7):1011-1019
Dendritic cells (DCs) are specialized antigen presenting cells specializing in antigen uptake and processing, and play an important role in the innate and adaptive immune response. A subset of bovine peripheral blood DCs was identified as CD172a+/CD11c+/MHC (major histocompatibility complex) class II+ cells. Although DCs are identified at 0.1%–0.7% of peripheral blood mononuclear cells (PBMC), the phenotype and function of DCs remain poorly understood with regard to maintaining tolerance during the pregnancy. All cattle used in this study were 1 month before parturition. We have established a novel method for the purification of DCs from PBMC using magnetic‐activated cell sorting, and purified the CD172a+/CD11c+ DCs, with high expression of MHC class II and CD40, at 84.8% purity. There were individual differences in the expressions of CD205 and co‐stimulatory molecules CD80 and CD86 on DCs. There were positive correlations between expression of cytokine and co‐stimulatory molecules in DCs, and the DCs maintained their immune tolerance, evidenced by their low expressions of the co‐stimulatory molecules and cytokine production. These results suggest that before parturition a half of DCs may be immature and tend to maintain tolerance based on the low cytokine production, and the other DCs with high co‐stimulatory molecules may already have the ability of modulating the T‐cell linage. 相似文献
33.
Shogo Moriya Shunpei Sato Moongeun Yoon Tomonori Azumaya Shigehiko Urawa Akihisa Urano Syuiti Abe 《Fisheries Science》2009,75(2):359-367
More than 1,000 age-identified chum salmon Oncorhynchus keta collected at 23 stations in the Bering Sea and the North Pacific Ocean in June to July 2003 were used to estimate their origin
of stocks using a DNA microarray developed for analyzing the mitochondrial (mt)DNA haplotypes. The observed haplotype distribution
was nearly the same as that reported previously for fish collected in September 2002 and 2003 in the present surveyed areas.
A conditional maximum-likelihood method for estimation of stock compositions indicated that the Japanese stocks mainly distributed
in north central Bering Sea, whereas the Russian stocks were mainly in western Bering Sea. The North American stocks were
abundant in eastern Bering Sea and around the Aleutian Islands. Such an area-specific stock composition was not significantly
different between mature and immature fish. Thus, the combined results of 2 years suggest that the distribution of chum salmon
is nonrandom in the surveyed areas in summer and autumn, and that fish of the same origin migrate together to the same area
irrespective of age. 相似文献
34.
Keishi Matsuda Shinsuke Torisawa Tomonori Hiraishi Katsutaro Yamamoto 《Fisheries Science》2009,75(1):35-42
In this study, we determined the color vision and spectral sensitivity of pointhead flounder, slime flounder, and red halibut
by recording electrophysiological S-potentials from isolated retinas; this basic data was collected to aid the selection of
appropriate colors and effective wavelengths for selective fishing gear for sustainable fisheries. Four kinds of L-response
and one C-response were recorded in the pointhead flounder and red halibut. Two kinds of L-response were recorded in the slime
flounder. The occurrence of a C-response indicates the possession of color vision. The C-responses show that the pointhead
flounder has superior distinction from blue to yellow, and red halibut has superior distinction from blue and green to red.
The most frequently recorded L-responses indicate high spectral sensitivity at 544 nm in pointhead flounder and 518 nm in
slime flounder and red halibut. All recorded L- and C-responses indicate sensitivity to ultraviolet wavelengths. 相似文献
35.
Kanefumi Kitahara Takako Tanaka Toshihiko Suganuma Tomonori Nagahama 《Cereal Chemistry》1997,74(1):1-6
The raw starch granules from corn, rice, and wheat were hydrolyzed by practically pure glucoamylase (Rhizopus niveus). The bound lipids remaining in the residual starches were investigated, of which the major components of the lipids, free fatty acids (FFA) in corn starch, FFA and phospholipids (PL) in rice starch, and PL in wheat starch were determined. In each case, the bound FFA and PL were decreased to some extent during the initial stage of hydrolysis. During the later stages, the FFA continued to gradually decrease, while the level of PL stabilized. It was interesting that some of the bound lipids were released from the granules upon glucoamylase hydrolysis, differing from the model amylose-lipid complexes. Furthermore, the structures of the residual starches were investigated. The blue value and λmax of the starches were increased by partial hydrolysis of the starch granules using practically pure glucoamylase. Two gel-permeation chromatography analyses revealed that the relative amount of amylose fraction was increased by glucoamylase hydrolysis, and also that the increments were reduced by the defatting of bound lipids. The results suggest that the increase in amylose fraction is attributable to the existence of bound lipids in the granules. 相似文献
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Kenji Tsuruta Tomonori Kume Hikaru Komatsu Naoko Higashi Toshihiro Umebayashi Tomo’omi Kumagai Kyoichi Otsuki 《Journal of Forest Research》2010,15(6):398-403
Sap flow techniques are practical tools for estimating tree transpiration. Though many previous studies using sap flow techniques
did not consider azimuthal variations of sap flux density (F
d) on xylem trunk to estimate tree transpiration, a few studies reported that ignoring the azimuthal variations in F
d could cause large errors in tree transpiration estimates for some tree species. Therefore, examining azimuthal variations
in F
d for major plantation tree species is critical for estimating tree transpiration. Using the thermal dissipation method, we
examined azimuthal variations in F
d in six trees of Japanese cypress Chamaecyparis obtusa (Sieb. et Zucc.) Endl., which is one of the most common plantation tree species in Japan. We recorded considerable variations
among F
d at four different azimuthal directions. The F
d value for one aspect was more than 100% larger than those for the other aspects. We calculated differences between tree transpiration
estimates based on F
d for one to three azimuthal directions and those based on F
d for four aspects. The differences relative to tree transpiration estimates based on F
d for four aspects were typically 30, 20, and 10% in accordance with the F
d for one, two, and three measurement aspects, respectively. This finding indicates that ignoring azimuthal variations could
cause large errors in tree transpiration estimates for Japanese cypress. 相似文献
39.
40.
Yaser Hosny Elewa Mohammad Hafez Bareedy Ahmed Awad Abu Al Atta Osamu Ichii Saori Otsuka Tomonori Kanazawa Shin-Hyo Lee Yoshiharu Hashimoto Yasuhiro Kon 《Veterinary research communications》2010,34(6):557-567
Previously, the distribution of myoepithelial cells (mecs) in the salivary glands was studied by both immunohistochemistry,
and transmission electron microscopy; however, little was elucidated concerning their morphological features, especially in
goats. This study was performed to investigate the correlation between the cytoarchitecture of the mecs in goat major salivary
glands (parotid, mandibular, and sublingual glands) and the nature of the saliva secretion. The cytoarchitectural features
of the mecs were examined by scanning and transmission electron microscopy as well as immunohistochemically. The secretory
endpieces in the parotid gland are of the pure serous type, but in both the mandibular and sublingual glands they are of the
mixed type. In all studied glands, the intercalated ducts were covered by mecs which, unlike the large stellate cells that
surrounded the secretory endpieces, were spindle-shaped with few cytoplasmic processes. Interestingly, the mecs were found
to bulge on the basal surfaces of the serous acini and intercalated ducts in all glands and to be in close contact to the
seromucous tubules surface in the mandibular and sublingual glands forming a continuous network around it. In conclusion,
the differences in the degree of development of the mecs as well as the number of their cytoplasmic processes may be correlated
with the nature of the secretion and the number of the secretory granules. Thus these observations may have some relevance
in the diagnosis of atrophy and pathogenic conditions of these glands. 相似文献