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921.
Iqbal El-Assaad Jeremy A. Di Bari Koji Yasuda Thomas J. Divers Brian A. Summers Alexander de Lahunta Hussni Mohammed 《Veterinary research communications》2012,36(4):221-226
Equine motor neuron disease (EMND) is a neurodegenerative disorder of unknown etiology affecting horses worldwide. Trans-Active Response DNA Binding Protein of 43?kDa (TDP-43) has been reported in the central nervous system (CNS) of several neurodegenerative conditions in humans including Amyotrophic Lateral Sclerosis (ALS) and assumed to play role in the disease. We examined whether horses afflicted with EMND express the TDP-43 in CNS. Ten horses with EMND and 6 controls of different ages and breed we enrolled. Detection of presence of TDP-43 protein in the CNS was analyzed by immunohistochemical staining using rabbit anti-human TARDBP (TDP-43) polyclonal antibody. Formalin fixed neuronal tissues from medulla, cervical, and lumbar spinal cord were harvested from EMND and from control horses. Sections were assigned randomly to TDP-43 treated or rabbit anti-IgG as control. Nuclear staining of TDP-43 was detected in one of the neural tissues of 75?% of EMND-positive and 0 of 0?% of control horses in the central nervous system (medulla, and/or cervical spinal cord and/or lumbar spinal cord). TDP-43 antibody was detected in the nucleus of EMND horses and no cytoplasmic staining was noted. As in ALS, there was no pattern of age clustering associated with the detection of TDP-43. This is the first report on the staining of TDP-43 in neuronal tissues of horses and suggests that TDP-43 may play a role in the pathogenesis of EMND. Further studies are needed to elucidate the etiologic role of this protein in the diseases. 相似文献
922.
Lane LV Meinkoth JH Brunker J Smith SK Snider TA Thomas J Bradway D Love BC 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2012,41(1):147-152
A 5-year-old female spayed Shetland Sheepdog Mix dog was evaluated for a history of recent seizure activity, progressive hind limb ataxia, polyuria, and polydipsia and no history of gastrointestinal signs. Physical examination findings included conscious proprioceptive deficits, ataxia, and anterior uveitis along with a hypermature cataract in the right eye. Results of a CBC, serum biochemical profile, urinalysis, and computed tomography scan of the brain were unremarkable. Cerebrospinal fluid (CSF) analysis revealed marked eosinophilic pleocytosis and rare organisms consistent with Prototheca spp within neutrophils and macrophages. On postmortem histologic examination, mononuclear inflammation and numerous intralesional algal organisms, similar to those seen on the cytologic preparation of CSF, were found in the brain, eyes, kidneys, and heart. Abnormalities were not detected on gross and histologic examination of the gastrointestinal tract. Cultures of CSF and subdural/olfactory bulb, but not intestinal tract, yielded growth of Prototheca spp, and PCR analysis and DNA sequencing confirmed the organism as Prototheca zopfii genotype 2. We have reported a rare case of disseminated protothecosis that was diagnosed by evaluation of CSF in a dog presented with neurologic signs and no overt enteric disease. Protothecosis should be considered as a rare cause of seizures, even in the absence of obvious enteric signs, and should be included in the differential diagnosis of eosinophilic pleocytosis. 相似文献
923.
Rigas JD Smith TJ Gorman ME Valentine BA Simpson JM Seguin B 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2012,41(1):141-146
An 8-year-old male neutered Pomeranian dog was presented to the Veterinary Teaching Hospital at Oregon State University for surgical treatment of hydronephrosis of the left kidney and a left cranial abdominal mass. A primary ureteral mass was found during exploratory surgery, and the mass was resected and ureteral anastomosis was performed. Cytologic evaluation of the mass revealed 3 distinct cell populations, including a large number of multinucleated giant cells, a moderate number of thin spindle-shaped cells, and cohesive clusters of transitional epithelial cells. The cytologic diagnosis was giant cell sarcoma. The diagnosis was confirmed by histologic examination, and immunohistochemical staining was performed. The spindle-shaped cells and multinucleated giant cells were both immunoreactive for vimentin and spindle-shaped cells for S-100. Tumor cells did not express wide-spectrum cytokeratin, broad-spectrum muscle actin, smooth muscle actin, sarcomeric actin, desmin, BLA36, Mac 387, synaptophysin, neuron-specific enolase, glial fibrillary acid protein, or von Willebrand factor. These findings are most consistent with an anaplastic sarcoma with giant cells. This is the first case report of a primary ureteral giant cell sarcoma in a dog. 相似文献
924.
The strength prediction of adhesively bonded timber joints is difficult due to the anisotropic and brittle nature of the adherends, the complex stress distribution as well as the uncertainties regarding the associated material resistance. This paper describes a probabilistic method for the strength prediction of balanced double lap timber joints. The method considers the statistical variation and the size effect in the strength of timber using a Weibull statistical function. The design method presents an explanation for the increased resistance of local zones subjected to high stress peaks as it takes into account not only the magnitude of the stress distributions, but also the volume over which they act. The predicted joint strengths are slightly underestimated compared with the experimental results due to inaccurate upper tail modelling of the material strength by the Weibull statistical distribution. The probabilistic method provides reasonable results for brittle joint failure and has immediate application in the design of adhesively bonded timber joints. 相似文献
925.
Geurden T Hodge A Noé L Winstanley D Bartley DJ Taylor M Morgan C Fraser SJ Maeder S Bartram D 《Veterinary parasitology》2012,189(2-4):308-316
The objective of the present studies was to evaluate the efficacy of a combined formulation (Startect(?) Dual Active Oral Solution for Sheep, Pfizer Animal Health) of derquantel (DQL) and abamectin (ABA) for the treatment of: (1) sheep experimentally infected with a moxidectin (MOX)-resistant isolate of Teladorsagia circumcincta, and (2) multi-drug resistant gastrointestinal nematode parasites under UK field conditions. In the first study, a total of 40 animals were allocated into 4 treatment groups, and were either left untreated or treated with DQL+ABA, MOX or ABA. Faecal samples were collected on days 1-5 and on day 7 after treatment to examine the reduction in faecal egg excretion and to evaluate the egg viability. On day 14 post treatment all animals were euthanised for abomasal worm counts. There was a 100% reduction in geometric mean worm counts for the DQL+ABA treated animals compared to the untreated control animals (P<0.0001), whereas the percentage reduction in worm counts for the MOX- (P>0.05) and ABA-treated (P=0.0004) animals was 12.4% and 71.8%, respectively. The data from the egg hatch assay (EHA) indicated that in the MOX-treated and the ABA-treated animals, the majority of the eggs hatched after treatment. In the field study, performed on four farms, animals were allocated into 6 groups of 11-15 animals each in order to conduct a faecal egg count reduction test (FECRT), based on arithmetic mean egg counts. One group of animals remained untreated, whereas the other animals were treated with DQL+ABA, MOX, fenbendazole (FBZ), levamisole (LV) or ivermectin (IVM). On each of the farms the reduction in egg excretion after treatment with FBZ, LV or IVM was below 95.0%, indicating anthelmintic resistance. The efficacy of DQL+ABA ranged from 99.1 to 100%, yielding significantly lower egg counts compared to the untreated control group (P≤0.003). For MOX the egg counts were significantly (P≤0.003) lower compared to the untreated group at each farm, with reductions varying from 98.2 to 100%. The post-treatment copro-cultures for larva identification indicated that T. circumcincta was the most abundant worm species after treatment (52-99% of the larvae). The results of these studies confirm the high efficacy of the DQL+ABA combination formulation against anthelmintic resistant nematodes in the UK. 相似文献
926.
Traversa D Castagna G von Samson-Himmelstjerna G Meloni S Bartolini R Geurden T Pearce MC Woringer E Besognet B Milillo P D'Espois M 《Veterinary parasitology》2012,188(3-4):294-300
This paper reports a survey conducted in France during 2011 to evaluate the efficacy of commonly used anthelmintics against horse cyathostomins. A total of 40 farms and 1089 horses were screened for the presence of cyathostomins. All farms but one were positive, with an overall animal infection rate of 53.7%, ranging from 9% to 83% on individual farms. On 445 horses from 30 of these farms, a faecal egg count reduction test (FECRT) was performed to evaluate the efficacy of oral formulations of fenbendazole (FBZ), pyrantel embonate (PYR), ivermectin (IVM) and moxidectin (MOX). Calculation of the mean FECR and 95% confidence intervals (95% CI) around the mean was performed using bootstrap analysis. Resistance to FBZ was found on 17 of 18 farms investigated, with a mean reduction of 57% (95% CI: 38.5-71.2%). Suspected resistance for PYR was found on 6 of 30 farms, and confirmed on another 3 of 30 farms, with a mean reduction for PYR of 94.7% (95% CI: 88.9-98.5%). Reduced efficacy simultaneously of FBZ and PYR was found in 7 farms. Reduced efficacy of IVM was found in one animal on one farm and of MOX in one animal on another farm, and was combined with resistance against FBZ and/or PYR. These results indicate that single and multiple drug resistance and reduced efficacy in equine cyathostomins is present in France. Macrocylic lactones proved to be highly effective compounds against cyathostomins, with reduced efficacy for IVM and MOX in two farms only. These results extend present knowledge on the occurrence of drug resistant cyathostomins in Europe, and illustrate the necessity to use anthelmintics in appropriate worm control programmes. 相似文献
927.
Jiang Jiang Donald L. DeAngelis Thomas J. Smith III Su Yean Teh Hock-Lye Koh 《Landscape Ecology》2012,27(1):109-119
Coastal vegetation of South Florida typically comprises salinity-tolerant mangroves bordering salinity-intolerant hardwood
hammocks and fresh water marshes. Two primary ecological factors appear to influence the maintenance of mangrove/hammock ecotones
against changes that might occur due to disturbances. One of these is a gradient in one or more environmental factors. The
other is the action of positive feedback mechanisms, in which each vegetation community influences its local environment to
favor itself, reinforcing the boundary between communities. The relative contributions of these two factors, however, can
be hard to discern. A spatially explicit individual-based model of vegetation, coupled with a model of soil hydrology and
salinity dynamics is presented here to simulate mangrove/hammock ecotones in the coastal margin habitats of South Florida.
The model simulation results indicate that an environmental gradient of salinity, caused by tidal flux, is the key factor
separating vegetation communities, while positive feedback involving the different interaction of each vegetation type with
the vadose zone salinity increases the sharpness of boundaries, and maintains the ecological resilience of mangrove/hammock
ecotones against small disturbances. Investigation of effects of precipitation on positive feedback indicates that the dry
season, with its low precipitation, is the period of strongest positive feedback. 相似文献
928.
929.
The response of cats to dietary sodium selenite (Na(2) SeO(3)) and organically bound selenium was studied in two separate studies with four cats per treatment and three levels of selenium supplementation (targets 1.0, 1.5 and 2.0 μg/g DM) for each Se source. Whole blood and plasma selenium concentrations and glutathione peroxidase (GPx) activity were determined at 7-time points across the 32-day study. Faeces were quantitatively collected during the last 8 days and urine was collected daily during both studies. The basal diet used had a low apparent faecal selenium absorption of 25.3 ± 3.0%. Daily faecal and urinary selenium excretion increased linearly with increasing selenium intake for both Se sources. Urinary selenium concentration of the cats fed the supplemented diets increased rapidly (~2 days) and remained constant throughout the remainder of the study. Apparent faecal selenium absorption was high for both selenium sources (73.2% and 80.0%). Plasma, and to a lesser extent, whole blood selenium concentrations increased in a dose-dependent manner with supplementation. Whole blood and plasma GPx activity were highly variable and showed a variable response to dietary selenium intake. Cats closely regulate selenium homeostasis through increasing urinary excretion whilst faecal absorption remains unaffected. 相似文献
930.
The objective of this study was to analyse the within-tree allocation of biomass and to develop biomass functions for above- and below-ground components of European beech in Denmark. Separate functions were developed for stem, branches, below-ground stump and root system, total above-ground biomass and total tree biomass. For each of these components or aggregate components, models were also developed for the average basic density of wood and bark. To enhance the versatility of the models, a function for estimating the biomass expansion factor (BEF) was also developed. The functions were based on 66 trees measured for total biomass. Model performance was evaluated based on 74 trees measured only for above-ground biomass. The trees were sampled in 18 different forest stands covering a wide range of tree sizes and stand treatments. Models were estimated using a linear mixed-effects procedure to account for within-stand correlations. The functions for biomass and BEFs included only diameter at breast height and total tree height for individual trees as predictor variables. Inclusion of additional variables reflecting site quality or stand density did not improve model performance. The functions for basic density included individual tree diameter, tree height and quadratic mean diameter as predictor variables, indicating an effect of stand density on the basic density of wood and bark. 相似文献