Difference in Amounts and Composition of Dew from Different Types of Dew Collectors

Dew was collected using several kinds of collectors at the same time, and the composition was compared. The volumes per unit surface areas of dew formed were different based on the types of collectors. It is found that the amounts of the ions, which in part come from gaseous substances, such as organic acids, nitrous acid, etc., per unit area in dew increase with dew volume per unit surface area. On the other hand, chloride, nitrate, sodium, potassium and magnesium which come from only particles showed no variation with the dew volume per unit surface area. These results suggest that the composition of dew varied by the dew volume per unit surface area formed in the different collectors. Dew is usually collected on a polytetrafluoroethylene (PTFE) sheet. The above result suggests that the dew concentration on the PTFE sheet does not represent the real dew concentration on various materials, such as leaves, cars, roofs, road, etc. To determine the real concentration of dew on a material, the material itself has to be usedas a collector. The PTFE sheet could be used as a standard dew sampler forcomparison of dew formed at various places.     

Cluster analysis to evaluate disease risk in periparturient dairy cattle

Predicting periparturient disease risk is of immense value to the dairy industry. Periparturient diseases are interrelated with each other; however, predicting the onset risk of these diseases has predominantly been based on a single blood parameter for a single disease. This study examined a new diagnostic method to predict the risk of periparturient diseases. We conducted cluster analysis of multiple blood constituents from 20 Holstein cattle at 1 week post‐partum, and the cattle were divided into two groups, A or B. We then compared the periparturient and early‐lactation blood constituents of these groups. Group B had significantly higher 3‐hydroxybutyric acid concentrations and were suspected to have subclinical ketosis. Group B also had significantly lower calcium concentrations, with a tendency for subclinical hypocalcemia. We also performed discriminant analysis using blood parameters at 1 week post‐partum, which grouped the population into the same two groups as the cluster analysis based on three variables: inorganic phosphorus, calcium, and either phospholipids or total cholesterol. We further showed that these discriminant functions could be used to predict the risk of periparturient disease even before parturition. Our results indicate that cluster analysis with multiple blood constituents is useful for predicting periparturient disease risks.     

Dose assessment of 2% chlorhexidine acetate for canine superficial pyoderma

The dose of 2% chlorhexidine acetate (2CA; Nolvasan^® Surgical Scrub; Fort Dodge Animal Health, Fort Dodge, IA, USA) for canine superficial pyoderma was evaluated. The first trial compared three doses (group 1, 57 mL/m² body surface area; group 2, 29 mL/m² body surface area; and group 3, 19 mL/m² body surface area) in a randomized, double‐blind, controlled fashion. Twenty‐seven dogs with superficial pyoderma were treated with 2CA at the allocated doses every 2 days for 1 week. The owners and investigators subjectively evaluated the dogs, and investigators scored skin lesions, including erythema, papules/pustules, alopecia and scales, on a 0–4 scale. There were no significant differences in response between the treatment groups. The second trial established a practical dose‐measuring method for 2CA. Sixty‐eight owners were asked to apply 2CA on their palm in an amount corresponding to a Japanese ¥500 coin, 26.5 mm in diameter. This yielded an average dose of 0.90 ± 0.40 mL. Mathematically, the doses used in groups 1, 2 and 3 can be represented as one coin per approximately one‐, two‐ and three‐hand‐sized lesions, respectively. The results therefore suggest that owners instructed to apply one coin of the product per two‐hand‐sized areas of superficial pyoderma would use the range of doses evaluated in this trial.     

Limb and skin abnormalities in mice lacking IKKalpha

The gene encoding inhibitor of kappa B (IkappaB) kinase alpha (IKKalpha; also called IKK1) was disrupted by gene targeting. IKKalpha-deficient mice died perinatally. In IKKalpha-deficient fetuses, limb outgrowth was severely impaired despite unaffected skeletal development. The epidermal cells in IKKalpha-deficient fetuses were highly proliferative with dysregulated epidermal differentiation. In the basal layer, degradation of IkappaB and nuclear localization of nuclear factor kappa B (NF-kappaB) were not observed. Thus, IKKalpha is essential for NF-kappaB activation in the limb and skin during embryogenesis. In contrast, there was no impairment of NF-kappaB activation induced by either interleukin-1 or tumor necrosis factor-alpha in IKKalpha-deficient embryonic fibroblasts and thymocytes, indicating that IKKalpha is not essential for cytokine-induced activation of NF-kappaB.     

Detection of a deep 3-microm absorption feature in the spectrum of Amalthea (JV)

Near-infrared spectra of Jupiter's small inner satellites Amalthea and Thebe are similar to those of D-type asteroids in the 0.8- to 2.5-micrometer wavelength range. A deep absorption feature is detected at 3 micrometers in the spectra of the trailing side of Amalthea, which is similar to that of the non-ice components of Callisto and can be attributed to hydrous minerals. These surface materials cannot be explained if the satellite formed at its present orbit by accreting from a circumjovian nebula. Amalthea and Thebe may be the remnants of Jupiter's inflowing building blocks that formed in the outer part or outside of the circumjovian nebula.     

Prediction of enteric methane emission from beef cattle in Southeast Asia

We conducted a meta‐data analysis to develop prediction equations to estimate enteric methane (CH₄) emission from beef cattle in Southeast Asia. The dataset was obtained from 25 studies, which included 332 individual observations on nutrient intakes, digestibilities, and CH₄ emissions. Cattle were provided tropical forage or rice straw, with or without concentrates in individual pens equipped with indirect open‐circuit head hood apparatus. The simplest and best equation to predict daily CH₄ emission was CH₄ (g/day) = 22.71 (±1.008) × dry matter intake (DMI, kg/day) + 8.91 (±10.896) [R² = 0.77; root mean square error (RMSE) = 19.363 g/day]. The best equation to predict CH₄ energy as a proportion of gross energy intake (CH₄‐E/GEI, J/100 J) was obtained using DMI per body weight (DMIBW, kg/100 kg), content (g/100 g DM) of ether extract (EE) and crude protein (CP), and DM digestibility (DMD, g/100 g); CH₄‐E/GEI = ?0.782 (±0.2526) DMIBW ? 0.436 (±0.0548) EE ? 0.073 (±0.0218) CP + 0.049 (±0.0097) DMD + 8.654 (±0.6517) (R² = 0.39; RMSE = 1.3479 J/100 J GEI). It was indicated that CH₄ emissions from beef cattle in Southeast Asia are predictable using present developed models including simple indices.     

Inhibitory effects of Ecklonia cava extract on high glucose-induced hepatic stellate cell activation

Nonalcoholic steatohepatitis (NASH) is a disease closely associated with obesity and diabetes. A prevalence of type 2 diabetes and a high body mass index in cryptogenic cirrhosis may imply that obesity leads to cirrhosis. Here, we examined the effects of an extract of Ecklonia cava, a brown algae, on the activation of high glucose-induced hepatic stellate cells (HSCs), key players in hepatic fibrosis. Isolated HSCs were incubated with or without a high glucose concentration. Ecklonia cava extract (ECE) was added to the culture simultaneously with the high glucose. Treatment with high glucose stimulated expression of type I collagen and α-smooth muscle actin, which are markers of activation in HSCs, in a dose-dependent manner. The activation of high glucose-treated HSCs was suppressed by the ECE. An increase in the formation of intracellular reactive oxygen species (ROS) and a decrease in intracellular glutathione levels were observed soon after treatment with high glucose, and these changes were suppressed by the simultaneous addition of ECE. High glucose levels stimulated the secretion of bioactive transforming growth factor-β (TGF-β) from the cells, and the stimulation was also suppressed by treating the HSCs with ECE. These results suggest that the suppression of high glucose-induced HSC activation by ECE is mediated through the inhibition of ROS and/or GSH and the downregulation of TGF-β secretion. ECE is useful for preventing the development of diabetic liver fibrosis.     

Progression of pancreatitis prior to diabetes onset in WBN/Kob-Lepr(fa) rats

We established the WBN/Kob-Lepr(fa) rat as a new congenic strain for the fa allele of the leptin receptor gene (Lepr). Homozygous (fa/fa) WBN/Kob-Lepr(fa) rats provide a model of non-insulin-dependent diabetes, although its onset is secondary to pancreatitis. In the present study, we compared histopathological observations of pancreatitis in each genotype of this rat, to examine its suitability as a model of pancreatitis. The histopathological findings of the pancreatitis revealed intense changes dependent on age, such as hemorrhage or hemosiderin deposition. The pancreatitis in homozygous (fa/fa) WBN/Kob-Lepr(fa) rats were more severe than those of WBN/Kob rats.     

Accumulation and localization of cesium in edible mushroom (Pleurotus ostreatus) mycelia

The characteristics of Cs accumulation and localization in edible mushrooms were examined using the mycelia of Pleurotus ostreatus-Y1. Scanning electron microscope images revealed the existence of white spots, and energy dispersive X-ray microanalyzer analysis indicated the presence of larger amounts of Cs and P in these spots in mycelia cultured on medium containing 25 mM CsCl. The (137)Cs activities in the mycelia were approximately 4-6 times higher than those in water used for (137)Cs elution. Higher Cs concentrations in the sediment fraction including vacuolar pellets were obtained compared to the upper fractions. It was observed that yellowish spots caused by the fluorescence of 4',6-diamidino-2-phenylindole (DAPI)-stained polyphosphate were localized in the mycelia. The higher fluorescence intensity of the yellowish-grained spots was measured in comparison with other regions in the mycelium. These results suggested that Cs in the mycelia was trapped by polyphosphate in vacuoles or other organelles.