光合细菌FP04的筛选及对中华鳖养殖水质和生产效益的影响

从入海排污口污泥中筛选到一株有较强净化养殖水质能力的光合细菌,初步鉴定其为红螺菌,命名为FP04。实验室试验表明,FP04能有效降低养殖污水的CODcr含量。在中华鳖的养殖水体中加入不同浓度的FP04,结果与对照组比较,FP04可显著降低养殖水体中的氨氮和化学需氧量,并可减少甲鱼病害发生,提高甲鱼养殖成活率和增重率,其中以养殖水体中FP04浓度为9×104CFU/mL的效果最好,成活率和增重率分别提高14%和31%。     

Impact of serial activation of embryonic pax2 and WT1 genes on transdifferentiation of renal tubular epithelial cells

AIM: To investigate the mechanisms of transdifferentiation of renal tubular epithelial cells by the observation of serial activation of embryonic pax2 and WT1 genes in chronic renal failure model of 5/6 nephrectomized injury. METHODS: Embryo of mice, cultured renal tubule cells and chronic renal failure rat model of 5/6 nephrectomized injury were established. The expressions of paired Box gene (pax2) and Willm’s tumor gene (WT1), as well as α-smooth muscle actin (α-SMA), a phenotype of mesenchymal cells, were detected by RT-PCR and immunohistochemistry techniques. RESULTS: (1) Expressions of pax2 and WT1 mRNA began at 11.5 d and 14 d of embryo and increased gradually, and expression in trace quantity at 2 weeks after birth. Immunohistochemistry analysis revealed that WT1 only expressed in the glomerular podocytes and expressions of pax2 and WT1 in adult renal tubular cells were negative. (2) Deno-expression of pax2 and WT1 in some tubular cells appeared at week 2 and peaked at week 4 in 5/6 nephrectomized rats, both showing a same trend of expression. However, pax2 showed another peak at week 10 afterwards. (3) Re-expressions of pax2 and WT1 in TEC at 0.5 and 24 h after treated with IL-1α (10 μg/L) or AngII (10-9 mol/L) were observed respectively, followed by upregulation of α-SMA expression, and mesenchymal cells characters were shown. The effects were inhibited by Ang II receptor 2 antagonist and WT1 antibody, respectively. CONCLUSION: Adult renal tubule cells acquire re-activation of embryonic pax2 and WT1 genes and phenotypes of mesenchymes when challenge with certain injuries. Deno-expression of pax2 and WT1 genes closely relates with high concentration of bioactive facors, such as AngII and IL-1, which are involved in the mechanisms of renal tubule cells transdifferentiation.     

散生指天小辣椒“满天星”的选育

满天星是以辣椒胞质雄性不育系E4A为母本,恢复系E28C为父本配制的中熟辣椒一代杂种。果实羊角形,纵径6～8cm,横径1．0-1．2cm,平均单果质量4．5g。青熟果绿色,老熟果鲜红色,辣味香浓。每667m。产鲜红椒1800～2400kg,干椒300～400kg。抗CMV和疫病,中抗TMV,适宜全国各地早春露地和保护地栽培。     

xPC环境下CMAC NN在淀粉生产线液位控制中的应用

在分析CMAC神经网络的基础上,提出了利用自适应CMAC神经网络来调节淀粉生产线中乳液的液位.通过对淀粉生产线中某一液位回路进行数学建模,然后对常规PID和自适应CMAC神经网络两种控制器进行了设计与仿真.仿真结果表明,自适应CMAC神经网络在控制效果上明显优于常规PID,并且具有很高的鲁棒性.在Matlab实时开发环境xPC Target下建立了液位实时控制系统,采用快速原型控制方式,具有系统组建方便、成本低、开发周期短等特点,该系统可实现精确的过程控制.     

灯泡体支撑件对贯流泵水力性能的影响

灯泡体支撑件(包括进人孔和底部支承)是贯流泵装置内部重要的过流部件,本文采用CFD方法对贯流泵装霞内部流场进行数值模拟,对不同支撑件形状进行了比较分析和优化,并与试验结果比较.结果表明,进人孔和底部支承的形状对泵装置的水力损失和水流流态的影响都比较明显,合理的支撑件形状可以改善流态、减少水流环量,提高泵装置效率.     

基于非接触式速度测量的排种驱动系统

介绍了一种利用非接触式速度测量装置驱动排种器的工作原理.该装置用前后两个光电传感器来探测路面的反射光信号,根据两路信号的时间差得到相对速度,并用该测速装置来驱动步进电机,步进电机带动排种器转动,以达到排种和速度同步的目的.此装置可以改善现有播种机靠地轮驱动时由于打滑造成的排种不均匀的问题,使排种更加均匀化.     

Immune responses and enhanced disease resistance in Chinese drum, Miichthys miiuy (Basilewsky), after oral administration of live or dead cells of Clostridium butyrium CB2

Clostridium butyrium CB2 isolated from chickens was tested as a potential fish probiotic in the Chinese drum, Miichthys miiuy . Fish were fed live (CB), dead CB2 (D-CB) cells (10⁸ cells g⁻¹) or spent culture supernatant (SCS), for 30 days and challenged with Vibrio anguillarum or Aeromonas hydrophila . Survival was higher in both the CB and the CB-D fed groups, but the SCS group was not significantly different from the control. After feeding live or dead CB2 cells, there was increase in phagocytic activity of the head kidney macrophages, the lysozyme activity of serum and gut mucosa and immunoglobulin (Ig) level. The SCS group showed no obvious change in immune parameters. The results suggest that live or dead Clostridium butyrium CB2 has an immunomodulatory effect on fish.     

麻竹笋用林施肥效果试验

对麻竹笋用林进行施肥试验研究,分析得知,鸡粪、复合肥、配方肥3种肥料对竹笋产量和经济纯收入有显著影响,均与竹笋产量、经济纯收入呈抛物线型关系,竹笋产量、经济纯收入随施肥量增加呈现先上升后下降的趋势;3种肥料的影响程度顺序依次为：配方施肥、鸡粪、复合肥,配方施肥的最佳施肥方案为鸡400＋复60＋硫酸锌0．9。     

浙江遂昌毛竹鞭笋丰产培育技术

为了发挥毛竹林生产潜力,增加竹农经济收入,在多年实践研究的基础上总结出一套毛竹鞭笋丰产培育技术。研究认为：在毛竹笋材两用林中采挖鞭笋,不会影响整体的竹鞭鞭径和竹鞭总量生长;毛竹林地覆盖稻草和砻糠能显著提高第3年的鞭笋产量;培育高产鞭笋的最佳施肥时间为每年的5月;鞭笋采收时间为5-10月。毛竹鞭笋丰产培育技术可在毛竹笋材两用林经营中推广应用。     

莰酮对蚜虫生物活性的研究

进行了樟树(CinnamomumcamphoraL.)中的莰酮(CamphorC10H160)对蚜虫的触杀、熏蒸毒力和拒食活性研究。结果表明,处理后24h莰酮对菜缢管蚜、桃蚜和棉蚜触杀作用的LD50分别为3.12×10-3,2.04×10-3,0.68×10-3 ng/头,熏蒸作用的LC50分别为3.44,2.56,1.06mg/L,拒食作用的AFC50分别为3.34,2.98,1.32mg/L。经回归和相关分析,处理浓度与作用效果相关性均达到极显著或显著水平。莰酮在田间防治菜缢管蚜的效果显著。