Metabolism of microbial nitrogen in ruminants with special reference to nucleic acids

Characteristically the metabolism of microbial nitrogen (N) compounds in ruminants involves the degradation of dietary N and synthesis of microbial protein (MP), compounds including a small amount of peptides and free amino acids, which may account for 75–85% of total N and the remainder are nucleic acids (NA: DNA and RNA). Generally rumen microbes contain 10–25% NA‐N of the total N while 70–80% is in the form of RNA. This paper describes the degradation and synthesis of NA in the rumen and their fate in the lower digestive tracts. Their physiological and nutritional significance in different types of ruminant animals is also discussed. The research works on NA metabolism in ruminants has been mainly on metabolism of purines after rumen microbial digestion and absorption in the lower gut. Subsequently, the fate of absorbed purines has been intensively investigated to assess the extent of MP synthesis in the rumen. The method for predicting ruminal synthesized MP and subsequently digested MP has been proposed using urinary purine derivative (PD) excretion in sheep and cattle fed on ordinary feed. The latter approach has now been adopted for calculation of protein supply in some feeding standards, although there are still difficulties in predicting representative samples of rumen microbes, and also uncertainties in variations of non‐renal and endogenous purine losses.     

Two cases of IgA multiple myeloma in the dog

Clinical and laboratory findings are presented from two cases of canine IgA multiple myeloma. One of these animals presented with typical clinical and pathological changes including haematological abnormalities and skeletal lesions with secondary invasion of other organs. In contrast, the primary focus of disease in the second dog was the anterior alimentary tract and there were no skeletal changes. In this respect this second case was similar to the condition in man known as alpha chain disease. The two paraproteins differed in electrophoretic mobility.     

Use of ev loci as a measure of inbreeding in domestic fowls

1. The endogenous avian leukosis virus (ev) loci present in 9 lines of domestic fowls have been partially characterised and the average heterozygosity of the loci in each line calculated. 2. Using these data an estimate of the coefficient of inbreeding of the lines was derived; this estimate of the extent of inbreeding is compared with the mating history of the lines. 3. This method provides the first means of directly assessing the degree of inbreeding of fowl lines: assumptions implicit in the method are discussed.     

A zinc-containing intraruminal device for facial eczema control in lambs

A zinc-containing intraruminal device has been developed for protecting lambs against facial eczema. The rate of release of zinc from the device has been optimised, and its safety in use established. Under both experimental and farm conditions, the device gave excellent protection against the liver injury associated with facial eczema. The device relies upon erosion for release of zinc, and disappears completely when its charge of zinc has been released, leaving no metal or plastic residue in the rumen. This device has the potential to greatly ameliorate the problem of facial eczema in New Zealand.     

Associations amongst three feline Porphyromonas species from the gingival margin of cats during periodontal health and disease

Digoxigenin labelled whole chromosomal DNA probes directed against three feline members of the genus Porphyromonas (P. gingivalis VPB 3492, P. circumdentaria NCTC 12469T and P. salivosa VPB 3313) were used to identify and quantify organisms in samples taken from the gingival margins of 40 domestic cats with different grades of periodontal disease. At the right upper canine tooth, the grade of periodontal disease ranged from 0 to 5 and the cfu of facultative/obligate anaerobes ranged from 5.5 x 10(4) to 2.0 x 10(6)). In 38 of the 40 cats, at least one of the three Porphyromonas species was isolated and regression analysis showed that the cfu of total Porphyromonas sp. was a highly significant indicator of the grade of periodontal disease (p < 0.001, R2 0.510). Feline P. gingivalis was isolated from 37 of the 40 cats and regression analysis showed that it was a highly significant predictor of the grade of periodontal disease (p < 0.001, R2 0.561). The cfu of P. salivosa was a significant predictor of the grade of periodontal disease (p < 0.001, R2 0.286) and regression analysis showed that there was a significant positive relationship between cfu of P. circumdentaria and grade of periodontal disease (p = 0.018, R2 0.116). The periodontal grades at the right upper third premolar tooth ranged from 0 to 6. The cfu of facultative/obligate anaerobes isolated ranged from 1.2 x 10(5) to 7.9 x 10(6), and regression analysis showed that cfu was a significant predictor of periodontal grade (p < 0.001, R2 0.378). The cfu of total Porphyromonas species ranged from 1.2 x 10(4) to 1.7 x 10(6) and regression analysis of the cfu against the grade of periodontal disease showed a highly significant association (p < 0.001, R2 0.633). The cfu of P. gingivalis ranged from 0 to 1.1 x 10(6) and regression analysis of the cfu of P. gingivalis against the grade of periodontal disease showed a highly significant association (p < 0.001, R2 0.439). The cfu of P. salivosa was a significant predictor of the grade of periodontal disease (p < 0.001, R2 0.479) and the same association was found between cfu of P. circumdentaria and grade of periodontal disease (p = 0.002, R2 0.204). This study has established Porphyromonas as anumerically significant and highly prevalent genus in feline periodontal disease.     

Duration of action and hemodynamic properties of mivacurium chloride in dogs anesthetized with halothane.

OBJECTIVE: To describe onset and duration of neuromuscular blockade induced by mivacurium chloride and its associated hemodynamic effects at 3 dosages in healthy dogs. ANIMALS: 7 Labrador Retrievers. PROCEDURE: Anesthesia was induced with thiopental and maintained with halothane in oxygen, and dogs were mechanically ventilated to end-tidal P(CO)2 between 35 and 40 mm Hg. Core temperature, end-tidal P(CO)2, and halothane concentration were kept constant throughout the experiment. Neuromuscular function was assessed by evaluation of the train-of-four response to a supramaximal electrical stimulus of 2 Hz applied to the ulnar nerve every 10 seconds. Blood for determination of plasma cholinesterase activity was obtained prior to administration of mivacurium, a bolus of which was administered IV, using a randomized Latin-square design for dosages of 0.01, 0.02, and 0.05 mg/kg of body weight. RESULTS: All dogs had typical plasma cholinesterase activity. After administration of mivacurium, differences were not evident between groups in heart rate, systolic, mean, or diastolic blood pressure, change at any time in heart rate, systolic, mean, or diastolic blood pressure, or pH. Interval from onset to 100% neuromuscular blockade was 3.92+/-1.70, 2.42+/-0.53, and 1.63+/-0.25 minutes at dosages of 0.01, 0.02, and 0.05 mg/kg, respectively. Duration of measurable neuromuscular blockade was 33.72+/-12.73, 65.38+/-12.82, and 151.0+/-38.50 minutes, respectively. Time of onset and duration of effect differed significantly among dosages. CONCLUSIONS AND CLINICAL RELEVANCE: Mivacurium provides good hemodynamic stability at the dosages tested. In dogs, this drug has a rapid onset and long duration of effect.     

Morphologic studies of uveoscleral outflow in normotensive and glaucomatous beagles with fluorescein-labeled dextran

Aqueous humor leaves the anterior chamber through 2 pathways: the trabecular meshwork of the iridocorneal angle and the unconventional uveoscleral route. In the latter route, aqueous humor leaves the anterior chamber, passes caudally through the trabecular meshwork and sclerociliary cleft to enter the supraciliary and suprachoroidal spaces. The fluid is absorbed by the choroidal and scleral circulation. Fluorescein-labeled dextran was slowly infused into the posterior chamber of healthy and glaucomatous Beagles for 30 minutes. The eyes were fixed in a mixture of 70% alcohol and formalin, placed in epoxy resin for histologic evaluation, and examined by fluorescent microscopy. Fluorescence was detected in the healthy dogs throughout the uveoscleral pathway. In the glaucomatous dogs, the uveoscleral outflow was decreased or completely absent.     

Digestibility of fiber components and reproductive performance of sows fed high levels of alfalfa meal

Two experiments were conducted with second-parity sows fed either 5 or 50% alfalfa meal diets (Exp. 1) or 5, 50 or 95% alfalfa meal diets (Exp. 2) beginning 30 d after breeding and continuing through lactation, 21 d postpartum. Diets in both experiments were calculated to be equal in crude protein, but different in metabolizable energy content. Feed intake was restricted to 2 kg/d during gestation and ad libitum during lactation. Sows were tethered in metabolism crates 45 d after breeding. Total feces were collected during two 5-d collection periods, 60 and 100 d after breeding. Digestibilities of dry matter, fiber components (neutral detergent fiber, acid detergent fiber, hemicellulose and cellulose), protein and energy were determined in both experiments for all diets and periods of gestation. In both experiments, there was a reduction (P less than .05) in digestibility of dry matter, fiber components, protein and energy with increasing fiber levels for both periods of gestation. In Exp. 1, the decrease in digestibility of dry matter, neutral detergent fiber, acid detergent fiber, cellulose and energy was greater at 100 d gestation than at 60 d in sows fed 50% alfalfa. In Exp. 2, 50 and 95% dietary alfalfa reduced 60-d digestibilities of dry matter, all fiber constituents, energy and protein; these digestibilities were further reduced (P less than .05) at 100 d of gestation. In Exp. 1, weight gains of sows fed 5 and 50% alfalfa diets from breeding to 109 d gestation, were 42 and 18 kg/sow (5 greater than 50% level, P less than .05).(ABSTRACT TRUNCATED AT 250 WORDS)