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81.
Aldair JW Pinto Maria M Figueiredo Fabiana L Silva Trycia Martins Marilene SM Michalick Washington L Tafuri Wagner L Tafuri 《Acta veterinaria Scandinavica》2011,53(1):67
Background
The aim of this study was to provide a systematic pathological and parasitological overview of the gastrointestinal tract (GIT), including the stomach, duodenum, jejunum, ileum, caecum and colon, of dogs naturally infected with Leishmania.Methods
Twenty mongrel dogs naturally infected with Leishmania (Leishmania) infantum and obtained from the Control Zoonosis Center of the Municipality of Ribeirão das Neves, Belo Horizonte Metropolitan area, Minas Gerais (MG) state, Brazil, were analyzed. The dogs were divided into two groups: Group 1 comprised nine clinically normal dogs and group 2 comprised 11 clinically affected dogs. After necropsy, one sample was collected from each GIT segment, namely the stomach, duodenum, jejunum, ileum, caecum and colon. Furthermore, paraffin-embedded samples were used for histological and parasitological (immunohistochemistry) evaluation and a morphometrical study were carried out to determine the parasite load (immunolabeled amastigote forms of Leishmania). The Friedman and the Mann Whitney tests were used for statistical analysis. The Friedman test was used to analyze each segment of the GIT within each group of dogs and the Mann Whitney test was used to compare the GIT segments between clinically unaffected and affected dogs.Results
The infected dogs had an increased number of macrophages, plasma cells and lymphocytes, but lesions were generally mild. Parasite distribution in the GIT was evident in all intestinal segments and layers of the intestinal wall (mucosal, muscular and submucosal) irrespective of the clinical status of the dogs. However, the parasite load was statistically higher in the caecum and colon than in other segments of the GIT.Conclusion
The high parasite burden evident throughout the GIT mucosa with only mild pathological alterations led us to consider whether Leishmania gains an advantage from the intestinal immunoregulatory response (immunological tolerance). 相似文献82.
83.
84.
Abias Santos Silva Luiz Gustavo Ribeiro Pereira Márcio dos Santos Pedreira Fernanda Samarini Machado Mariana Magalhães Campos Cristina Simões Cortinhas Tiago Sabella Acedo Rafael Dantas dos Santos João Paulo Pacheco Rodrigues Rogério Martins Maurício Thierry Ribeiro Tomich 《Journal of animal physiology and animal nutrition》2020,104(1):76-87
The effect of exogenous amylase on the in vitro rumen digestion kinetics of whole-crop maize silage made from dent (RB9004) or flint grain type (RB9308) was evaluated at different phenological stages: soft dough (SOD), early dent (EAD), ½ milkline (½M) and ¾ milkline (¾M). Forage was harvested from 70 to 110 days after sowing. Two rumen-cannulated cows receiving or not exogenous amylase (0.7 g/kg dry matter—DM, provided to achieve 396 kilo Novo units of amylase activity/kg of TMR DM) were used as donor of ruminal fluid. The in vitro gas production kinetics was evaluated according to a dual-pool logistic model. The chemical composition and gas production kinetics were affected by the hybrid and phenological stages. The flint hybrid had lower range for chemical analysis among physiological stages. Harvesting at ½M and ¾M improved DM content, bromatological composition and silage quality parameters compared to dent or flint types. Amylase (i) increased methane (CH4) production and in vitro dry matter digestibility (IVDMD) in ½M stage, (ii) improved digestion kinetics by reducing lag time and increasing total gas production and fermentation rates of non-fibrous carbohydrates (NFC) and fibrous carbohydrates (FC), and (iii) increased extent and fermentation rate of NFC and increased fermentation rate of FC fraction in whole-crop maize silages produced from dent or flint types in all phenological stages. Harvesting between ½M and ¾M is the best phenological stage to improve chemical composition and silage quality parameters. Exogenous amylase showed improvements on fibre digestion of silages at ½M and ¾M phenological stages in both grain types of corn. 相似文献
85.
Pmila Carolini Gonalves da Silva Camila Celeste Brando Ferreira Ítavo Luís Carlos Vinhas Ítavo Marina de Nadai Bonin Gomes Gelson Luís Dias Feij Kedma Leonora Silva Monteiro Ferelli Natlia da Silva Heimbach Jonilson Araújo da Silva Gleice Kelli Ayardes de Melo Marília Williani Filgueira Pereira 《Animal Science Journal》2020,91(1)
Our hypothesis suggests an improvement in carcass and meat quality of pasture‐finished animals by introducing concentrate feed into diets. This study aimed to evaluate the effect of different levels of protein‐energy supplementation on carcass and meat characteristics of Texel lambs raised on Brachiaria pastures, and compare the results with those obtained from confined animals. Thirty 2‐month‐old intact lambs were divided into five treatments: pasture with mineral supplementation, pasture containing daily supplies of 0.8, 1.6, and 2.4% BW protein‐energy supplementation, and confinement (reference treatment). All animals were slaughtered 104 days after the beginning of the experiment. Lambs submitted to 1.6% and 2.4% BW supplementation presented similar characteristics to animals kept in confinement and were superior to animals treated with 0 or 0.8% of BW supplementation levels (p < .05). Increases of subcutaneous fat thickness were verified for 0.0, 0.8, 1.6, 2.4% BW, and confinement treatments, being respectively of 0.25, 0.74, 1.61, 1.69, and 1.98. Conversely, treatments had no influence on meat physical‐chemical characteristics, being all considered moderately soft, juicy (tender), and with mild tastes and odors. Lambs raised on Brachiaria pastures have high‐quality meat, but more protein‐energy supplementation is needed to be an alternative for the feedlots for young animals. 相似文献
86.
J C Silva S Ogassawara M F Marvulo J S Ferreira-Neto J P Dubey 《Journal of zoo and wildlife medicine》2001,32(3):349-351
Serum samples from 37 captive exotic felids in 12 zoos from six Brazilian states were assayed for antibodies to Toxoplasma gondii by the modified agglutination test using formalin-fixed whole tachyzoites. Titers greater than or equal to 1:20 were considered positive. Antibodies to T. gondii were found in 24 of 37 (64.9%) felids, including one European lynx (Lynx lynx), two jungle cats (Felis chaus), two servals (Leptailurus serval), two tigers (Panthera tigris), three leopards (Panthera pardus), and 14 of 27 lions (Panthera leo). This is the first serologic analysis for T. gondii infection in exotic wild felids from Brazilian zoos. 相似文献
87.
88.
Marie E Pinkerton Keith L Bailey Kathy K Thomas Thomas E Goetz Victor E Valli 《Journal of veterinary diagnostic investigation》2002,14(2):150-152
A 17-year-old Quarterhorse gelding with a clinical diagnosis of protein-losing enteropathy was submitted for necropsy following a 4-5-month duration of weight loss, decreased appetite, and hypoproteinemia. Gross findings included multiple 1-2-cm diameter ulcers on the luminal surfaces of the duodenum and ileum. Histologic examination revealed individual large, round cells infiltrating much of the mucosal epithelium of the duodenum, jejunum, ileum, and colon in addition to multifocal areas of ulceration. Similar round cells infiltrated Brunner's glands and expanded the submucosa beneath the foci of ulceration. Immunohistochemical staining indicated the round cell population was of T-lymphocyte origin. Several features of this equine neoplasm bear similarities to enteropathy-associated T-cell lymphoma in humans. 相似文献
89.
Circulating antibody against Fasciola hepatica antigens was determined by enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis in alpacas naturally exposed to F. hepatica. Serological assay parameters were established by using sera from eight infected animals and seven controls with no record of this parasitic infection. Excretory--secretory (ES-) products, Fas1- and Fas2-ELISA were used to survey 307 alpacas from a F. hepatica endemic area in the Peruvian Andes. Seroprevalence of F. hepatica infection varied from 56.7, 64.8 and 66.8% measured by Fas1-, Fas2- and ES-ELISA, respectively. The sensitivity for ES-ELISA was 95%, corresponding Fas1- and Fas2-ELISA sensitivity values were 90 and 95%. In this population, 7% of animals were positive for F. hepatica eggs in faeces, other parasites detected were Trichuris sp. (40%), Nematodirus sp. (34.6%), Lamanema sp. (12.8%) and Eimeria sp. (11.8%). The results show that F. hepatica infected animals elicit circulating antibodies against ES, Fas1 and Fas2. Fas2-ELISA may be proposed as a sensitive assay for the immunodiagnosis of fasciolosis in alpacas. 相似文献
90.
HNKS Pathirana Gee-Wook Shin SHMP Wimalasena BCJ De Silva Sabrina Hossain Gang-Joon Heo 《Journal of Exotic Pet Medicine》2018,27(4):67-73
Proteus spp. are widely recognized as opportunistic pathogens causing urinary tract and septic infections in humans and animals. The aim of this study was to investigate the prevalence of plasmid-mediated quinolone resistance genes and mutations in the quinolone resistance determining region in association with the detection of quinolone susceptibility of 24 strains of pet turtle-borne Proteus spp. Susceptibility of 4 antimicrobials including nalidixic acid, ciprofloxacin, ofloxacin, and levofloxacin was examined by disk diffusion and minimum inhibitory concentration test. Six isolates were resistant to nalidixic acid showing either intermediate resistance or resistance to other quinolones. All nalidixic acid, resistant isolates harbored mutations in gyrB (N440T/A401G/Q411S). Two of the isolates had both gyrA (S83I) and parC (S80I) mutations. Twenty-one isolates were positive for the presence of plasmid-mediated quinolone resistance genes; the qnrD gene had the highest prevalence with 19 (79.2%), while qnrS, qnrA, qnrB, and aac(6′), Ib-cr genes were present in 9 (37.5%), 2 (8.3%), 1 (4.2%), and 11 (45.8%) isolates, respectively. These results suggest that pet turtle-associated Proteus spp. should be considered a potential source of antimicrobial resistance determinants. 相似文献