Combined effects of water temperature and daily food availability period on the growth and survival of tench (Tinca tinca) larvae

A 16‐day experiment was designed to find the best combination of water temperature (27, 30, 33°C) and daily duration of food availability (12, 18, 24 h) for larval tench (Tinca tinca) growth and survival. Larvae with an initial mean size of 5.7 mm total length (TL) and 0.7 mg wet body weight (BW) were stocked at 15 L^?1. Larvae were fed in excess with live Artemia nauplii with the period of food availability lasting 12, 18 or 24 h daily. The largest final larval size was recorded at 27 and 30°C in groups fed for 24 h a day (17.7 and 17.9 mm TL, 76.1 and 77.7 mg BW, respectively). The combination of the highest temperature and the longest daily food availability was the only set of conditions under which final larval survival was affected (95.4% survival; 98.7–99.9% under all other conditions). The combination of water temperature of about 28.6°C and continuous food availability is recommended as the optimum combination for rearing T. tinca larvae under controlled conditions. Providing continuous food supply to fish larvae under aquaculture conditions was also advantageous in helping to mitigate the effects of slower growth relative to developmental progress, which can occur at high water temperatures. However, should one wish to limit the daily feeding period to 12 h per day, the use of a water temperature between 27.4 and 27.9°C would be the best solution.     

The impact of restoration processes on the selected soil properties and organic matter transformation of mountain fens under <Emphasis Type="Italic">Caltho-Alnetum</Emphasis> community in the Babiogórski National Park in Outer Flysch Carpathians,Poland

Purpose

The objective of this study was to determine the impact of restoration processes on the selected soil properties and organic matter transformation of mountain fens under the Caltho-Alnetum community in the Babiogórski National Park in Outer Flysch Carpathians.

Materials and methods

Restoration processes were conducted on three degraded mountain fens in the Babiogórski National Park in Outer Flysch Carpathians, Poland. The degradation degree of soils was the criterion for the selection of habitats for further studies. To determine the influence of restoration processes on mountain fen soil properties and organic matter transformation, samples were collected in 2011 and 2013. The soil samples were assayed for pH, base cation concentration, hydrolytic acidity, organic carbon and total nitrogen content, total exchangeable base cation concentration, cation exchange capacity, and base saturation. Organic matter fractions were extracted by IHSS method. Quantitative and qualitative study of organic matter was based on fraction composition analysis and the ratio of humic acid carbon to fulvic acid carbon. The research results were statistically verified.

Results and discussion

Based on morphological and chemical properties, the studied mountain fen soils can be classified as Sapric Dranic Eutric Histosols and Sapric Dranic Dystric Histosols according to WRB guidelines (2015). Before restoration processes, the mountain fen soils subjected to a different water regime showed various contents of total nitrogen and organic carbon. The decreasing of the groundwater level was reflected in pH, calcium ion content, exchangeable base cation concentration, and base saturation. The increase of the groundwater level had influence on chemical properties of mountain fen soils such as pH, total exchangeable base cation concentration, hydrolytic acidity, cation exchange capacity, and base saturation. Three-year restoration processes did not cause significant changes in the composition of humic substance fractions.

Conclusions

Mountain fens under Caltho-Alnetum community are priority habitats in Babiogórski National Park in Outer Flysch Carpathians, Poland. These habitats responded to restoration processes in varying degrees depending on the extent of their degradation. The least degraded mountain fen was characterized by a short response time on the restoration processes. The reaction of higher degraded habitats was weaker.

     

Urban soil resources of medium-sized cities in Poland: a comparative case study of Toruń and Zielona Góra

Purpose

Despite the many studies of urban soils, a comparative analysis for cities of a similar size has not yet been conducted. Thus, the aim of this review paper was to compare the soil distribution patterns in the area of two medium-sized Polish cities (Toruń and Zielona Góra). The authors attempted to answer the question of how natural and technogenic factors contributed to the transformation of urban soils and what the similarities and differences are between these two studied cities.

Materials and methods

First, both the natural and the human-related (including historical) factors influencing the soil formation in the studied cities were analysed. Then, a comparison of the degree of transformation of the urban soil environment was presented. The data obtained by the authors during nearly two decades of research (over 200 soil profiles) were used.

Results and discussion

Intensive development of the built-up areas in Toruń brought heavy and long-term transformations of soils, which demonstrate the typical properties of Urbic Technosols, Ekranic Technosols and other technogenic soils. Zielona Góra showed a similar state of soil transformation over a considerably smaller area. Currently, the differences in the soil properties in many built up areas have been blurred, despite the habitat and historical base. The similarities of the soil properties concerned, in particular, a high content of skeletal remains (from a few to over 30%), elevated pH (in KCl) values (even above 8.0) and the artificial soil horizons formation. Both cities struggle with similar problems regarding the changes in the land use within the areas covered by these soils.

Conclusions

It was found that, despite the significant habitat and historical differences between the two studied cities, most of the urban soils, especially Urbic Technosols, Ekranic Technosols and Regosols (Relocatic and Technic), are characterised by similar morphology and properties. The most important differences are the time and scale of the area transformation, which influence the extent of Technosols and Anthrosols within the city borders. The most distinct differences concern the natural and slightly transformed soils, which are the results of various soil-forming factors.

     

How the deadwood of different tree species in various stages of decomposition affected nutrient dynamics?

Purpose

The aim of the study was to estimate how the deadwood of different tree species in various stages of decomposition affected nutrient dynamics.

Materials and methods

The deadwood of eight species (common alder, common aspen, common ash, silver fir, pedunculate oak, Norway spruce, common hornbeam and silver birch) was selected. Three logs from each species in the third, fourth and fifth decay classes were chosen for analysis. Wood in the third decay class was characterised by larger hard fragments, fragmented bark and no branches; in the fourth decay class, it was characterised by small pieces and a fragmented bark; and in the fifth decay class, it was characterised by a soft texture and no bark. The investigation was carried out in the Czarna Rózga Reserve in Central Poland. Tension lysimeters were installed under each log in the humus horizon. The water samples collected from tension lysimeters were chemically analysed in the laboratory. The water chemistry was analysed by means of ion chromatography using a DIONEX ICS 5000 unit.

Results and discussion

The leachate obtained from different tree species contained different ion concentrations. The high similarity of the concentration of total anions and statistically significant differences in the content of total cations were determined in the leachate from the wood of coniferous and deciduous species. The concentration of the cations increases with the advancement of the decomposition level. A general linear model analysis demonstrates that wood species and the decomposition classes are of equal importance in defining the ion composition of the filtrate leaching from deadwood.

Conclusions

Wood at the highest decomposition stage releases more ions to the surface soil layers than wood at the lower decay class. An exception from the rule is the wood of birch, which in the III decay class releases more ions than in its higher decay classes. When comparing the ionic composition of leachate released from wood of coniferous and deciduous tree species, the latter are characterised by higher cation concentrations in comparison with coniferous species. Among the deciduous species, wood of such species as ash, hornbeam, aspen, birch and alder has the most favourable effects on the soil surface horizons through its supply with ionic substances. The ash wood releases high amounts of calcium, hornbeam wood releases magnesium and sodium, and aspen releases calcium, potassium and nitrate anion. From the analysed coniferous species, fir wood has a more favourable effect in terms of ion release to soil than spruce wood.

     

Transformation of beech forest litter as a factor that triggers arsenic solubility in soils developed on historical mine dumps

Purpose

Soils that develop on the dumps in historical arsenic mining sites contain high concentrations of As thus constituting a serious environmental risk. This study was aimed to examine the changes in arsenic solubility in mine soils as induced by organic matter introduced with forest litter.

Materials and methods

Four large samples of initially developed soils were collected from the dumps remaining in former mining sites and were incubated for 90 days at various moistures: 80% of maximum water holding capacity and 100% (flooded conditions), with and without addition of beech forest litter (BL), 50 g/kg. Soils contained up to 5.0% As. Soil pore water was collected periodically with MacroRhizon suction samplers and examined on As, Mn, and Fe concentrations, pH, Eh, and dissolved organic carbon (DOC). The properties of dissolved organic matter were characterized by UV-VIS spectroscopic parameters A4/A6 and SUVA₂₅₄.

Results and discussion

Application of BL resulted in an intensive release of As from soils, particularly at 100% moisture. As concentrations in soil pore water increased strongly during the first 2 or 4 weeks of incubation and then started to decrease in all cases, except for one flooded soil. As was released particularly intensively from carbonate-containing soils. The mechanisms of As mobilization, including reductive dissolution of Mn and Fe oxides and the competition with DOC for sorption sites on the oxides, were discussed as related to soil properties. Pore water concentrations of DOC were increasing at the beginning of incubation and started to decrease after two or four weeks. Spectroscopic parameters of dissolved organic matter in ZS soils indicated increasing aromaticity and progress of humification.

Conclusions

Forest litter introduced to mine dump soils causes a mobilization of As into soil pore water. This effect, particularly strong in carbonate-rich soils, is apparently related to high concentrations of DOC and usually declines with time, which may be explained by the progress in humification. The relationships between DOC properties and As speciation in soil pore water should be dissected for better interpretation of experimental results.

     

Preparation of biologically active monomeric recombinant zebrafish (<Emphasis Type="Italic">Danio rerio</Emphasis>) and rainbow trout (<Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>) recombinant growth hormones

Fish growth hormones (GHs) play an important role in regulating growth, metabolism, reproduction, osmoregulation, and immunity and have thus garnered attention for their application in aquaculture. Zebrafish GH (zGH) cDNA or rainbow trout GH (rtGH) cDNA was cloned into the pMon3401 vector, expressed in MON105-competent Escherichia coli and purified to homogeneity. Their biological activity was evidenced by their ability to interact with ovine GH receptor extracellular domain and stimulate GH receptor-mediated proliferation in FDC-P1-3B9 cells stably transfected with rabbit GH receptor. The relative affinity of zGH and rtGH, estimated by IC₅₀, was about 38-fold and 512-fold lower, respectively, than ovine GH. This is likely the reason for the low biological activity in cells with rabbit GH receptor, ~?36-fold lower for zGH and ~?107-fold lower for rtGH than for human GH. This was not due to improper refolding, as evidenced by circular dichroism analysis. Predicting the activity of fish GHs is problematic as there is no one single optimal in vitro bioassay; heterologous assays may be ambiguous, and only homologous assays are suitable for measuring activity.     

Diversity of Avr‐vnt1 and AvrSmira1 effector genes in Polish and Norwegian populations of Phytophthora infestans

The oomycete Phytophthora infestans, the cause of late blight, is one of the most important potato pathogens. During infection, it secretes effector proteins that manipulate host cell function, thus contributing to pathogenicity. This study examines sequence differentiation of two P. infestans effectors from 91 isolates collected in Poland and Norway and five reference isolates. A gene encoding the Avr‐vnt1 effector, recognized by the potato Rpi‐phu1 resistance gene product, is conserved. In contrast, the second effector, AvrSmira1 recognized by Rpi‐Smira1, is highly diverse. Both effectors contain positively selected amino acids. A majority of the polymorphisms and all selected sites are located in the effector C‐terminal region, which is responsible for their function inside host cells. Hence it is concluded that they are associated with a response to diversified target protein or recognition avoidance. Diversification of the AvrSmira1 effector sequences, which existed prior to the large‐scale cultivation of plants containing the Rpi‐Smira1 gene, may reduce the predicted durability of resistance provided by this gene. Although no isolates virulent to plants with the Rpi‐phu1 gene were found, the corresponding Avr‐vnt1 effector has undergone selection, providing evidence for an ongoing ‘arms race’ between the host and pathogen. Both genes remain valuable components for resistance gene pyramiding.     

Comparative Study of Predominant Phytochemical Compounds and Proapoptotic Potential of Broccoli Sprouts and Florets

The study compares lyophilized broccoli sprouts and florets in terms of their chemical composition, cytotoxic and proapoptotic potential against hepatocellular carcinoma HepG2, colorectal cancer SW480, and skin fibroblast BJ cells. Sinapic and isochlorogenic acids were predominant phenolics in the sprouts and florets, respectively. The amount of sulforaphane in the sprouts was significantly higher vs. florets. Oleic and linoleic acids dominated in the sprouts, while caproic, stearic and oleic acids in the florets. Broccoli sprouts were selectively cytotoxic on HepG2 and SW480 cells, with proapoptotic effect for the latter, while the florets were less selective, but more active, with profound proapoptotic effect for HepG2 cells (77.4%). Thus, lyophilized broccoli sprouts may be effectively used in dietary chemoprevention.