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61.
The dependence of the acidolysis reaction of a C6-C3 dimeric nonphenolic β-O-4 type lignin model compound, 2-(2-methoxyphenoxy)-1-(3,4-dimethoxyphenyl) propane-1,3-diol (veratrylglycerol-β-guaiacyl ether, VG), on the type of acid applied was examined using three different acids [0.2 mol/l HCl, 0.2 mol/l HBr,
and 0.1 mol/l (0.2 N) H2SO4 in 82% aqueous 1,4-dioxane at 85°C]. In the HCl system, the major reaction modes of the corresponding benzyl cation-type
intermediate (BC), which is produced by protonation of the α-hydroxyl group of VG and successive release of the water molecule, are the abstraction of the β-proton and hydride transfer from the β-to the α-position. The liberation of formaldehyde from the γ-hydroxymethyl group of BC is the predominant reaction mode in the H2SO4 system. Apparently, an unknown reaction mode or modes is operative in the early stage of the HBr system that causes rapid
disappearance of VG accompanied by the quantitative formation of 2-methoxyphenol without affording the common counterpart
of a Hibbert’s ketone, 1-hydroxy-3-(3,4-dimethoxyphenyl) propan-2-one. The reaction mode in the HBr system changes with the
progress of the reaction and is the same as that in the HCl system after the early stage. 相似文献
62.
Hoshi H Yagi Y Iijima H Matsunaga K Ishihara Y Yasuhara T 《Journal of agricultural and food chemistry》2005,53(23):8948-8956
Tricholoma matsutake, a high-class edible mushroom in Japan, has been reported to have excellent biological activities, but difficulty in cultivating the fruit bodies and limited bulk availability have restricted detailed studies. We have developed a method of culturing in tanks, enabling the bulk supply of the mycelia. The preparation (CM6271) exerts modulative effects on the immune competence of mice and rats. In this study, a sodium hydroxide extract of CM6271 was defatted followed by fractionation with a combination of ion exchange chromatography and gel filtration in order to identify the components involved in the expression of the activity, and a single peak fraction (MPG-1) was obtained with reversed phase chromatography. MPG-1 was a glycoprotein (sugar:protein ratio, 94.3:5.7) with a relative molecular mass of 360 kDa, and the sugar moiety contained about 90% glucose. NMR spectra and methylation analysis revealed that the alpha-1,4-linkage was the predominant glucan linkage with alpha-1,6- and alpha-1,2-linkages in the minority. The amino acid composition in the protein moiety was rich in glutamine, alanine, asparagine, leucine, glycine, valine, serine, threonine, isoleucine, and proline. MPG-1 was resistant to degradation with amylase or protease. The oral administration of MPG-1 promoted, in a dose-dependent manner, the recovery of the mouse natural killer cell activity and serum IL-12 level that had been reduced by the loading of restraint stress. The dose of MPG-1 (25 mg/kg) required for the expression of the effect decreases to 1/12 of that of CM6271 (300 mg/kg). Furthermore, MPG-1 formed a complex with TGF-beta1 in vitro, modulating the biological activity of TGF-beta1 by binding to its active form. These results indicate that the mycelium of T. matsutake contains a novel alpha-glucan-protein complex with immunomodulatory activities. 相似文献
63.
Sato M Ishikawa T Ujihara N Yoshida S Fujita M Mochizuki M Asada A 《Science (New York, N.Y.)》2011,332(6036):1395
The moment magnitude (M(w)) = 9.0 2011 Tohoku-Oki mega-thrust earthquake occurred off the coast of northeastern Japan. Combining Global Positioning System (GPS) and acoustic data, we detected very large sea-floor movements associated with this event directly above the focal region. An area with more than 20 meters of horizontal displacement, that is, four times larger than those detected on land, stretches several tens of kilometers long along the trench; the largest amount reaches about 24 meters toward east-southeast just above the hypocenter. Furthermore, nearly 3 meters of vertical uplift occurred, contrary to observed terrestrial subsidence. 相似文献
64.
65.
The residence times of the soluble fractions of beryllium and aluminum in seawater are estimated to be 1500 years or more. These residence times are estimated from a comparison of the annual deposition rates of cosmogenic beryllium-10 and aluminum-26 with the concentrations of beryllium-10 and aluminum-26 in seawater estimated from the specific activities of these radionuclides in an authigenic mineral assembly such as a manganese nodule. These residence times are greater by an order of magnitude than the mean residence times of beryllium and aluminum estimated from the geochemical balance. 相似文献
66.
Brown MG Dokun AO Heusel JW Smith HR Beckman DL Blattenberger EA Dubbelde CE Stone LR Scalzo AA Yokoyama WM 《Science (New York, N.Y.)》2001,292(5518):934-937
Natural killer (NK) cells are lymphocytes that can be distinguished from T and B cells through their involvement in innate immunity and their lack of rearranged antigen receptors. Although NK cells and their receptors were initially characterized in terms of tumor killing in vitro, we have determined that the NK cell activation receptor, Ly-49H, is critically involved in resistance to murine cytomegalovirus in vivo. Ly-49H requires an immunoreceptor tyrosine-based activation motif (ITAM)-containing transmembrane molecule for expression and signal transduction. Thus, NK cells use receptors functionally resembling ITAM-coupled T and B cell antigen receptors to provide vital innate host defense. 相似文献
67.
68.
Lycopene accumulation induced by 2-(4-chlorophenylthio)-triethylamine hydrochloride 总被引:11,自引:0,他引:11
After fruits, roots, or the mycelium of certain plants were treated with 2-(4-chlorophenylthio)-triethylamine hydrochloride, lycopene was detected in the tissue. This is the first known success in causing lycopene to accumulate in a wide range of carotenogenic tissues that normally do not accumulate the pigment at some stage of development. The response should be of value in the study of carotenoid biosynthetic pathways and gene control mechanisms. 相似文献
69.
70.
Furuuchi R Yokoyama T Watanabe Y Hirayama M 《Journal of agricultural and food chemistry》2011,59(8):3738-3746
Proanthocyanidins and other polyphenols in the seeds and juice of boysenberry were quantitatively analyzed. Polyphenolic extracts were prepared from the waste seeds and commercial juice by chromatographic fractionation. Compositional analysis revealed that both extracts contained six polyphenolic classes: flavanol monomers, proanthocyanidins, anthocyanins, ellagic acid, ellagitannins, and flavonol glycosides. Ellagitannins were the most abundant polyphenols in both extracts. Proanthocyanidins were present as short oligomers consisting of dimeric and trimeric procyanidins and propelargonidins, with the most abundant component being procyanidin B4 in both extracts. Quantification by high-performance liquid chromatography-mass spectrometry (HPLC-MS) revealed that the seeds contained a 72-fold higher amount of proanthocyanidins than the juice. These results indicate that boysenberry fruits contain short oligomeric proanthocyanidins along with flavanol monomers and the seeds represent a good source of short oligomeric proanthocyanidins. 相似文献