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71.
Kensuke ARAKAWA Yasushi KAWAI Kenji FUJITANI Junko NISHIMURA Haruki KITAZAWA Ken-ichi KOMINE Kenzo KAI Tadao SAITO 《Animal Science Journal》2008,79(5):634-640
The use of bacteriocins from Lactobacillus gasseri , a probiotic lactic acid bacterium, as bio-preservatives in the food industry and animal formulations has been limited because few strains of Lb. gasseri are cultivated and produce a bacteriocin in natural media such as milk and milk-based media. By the determination of the growth-supplements to milk among the 47 nutrients, Lb. gasseri JCM1131T , LA39 and LA158 isolated from human feces were successfully cultured in reconstituted skim milk and cheese whey using proteose peptone as a nutrient supplement, where Lb. gasseri LA39 produced a useful bacteriocin, gassericin A, with effective growth-inhibiting activity against Gram-positive food-borne pathogens. The data suggest these developed low-cost safe media supporting enough production of bacteriocins by the probiotic Lb. gasseri LA39 could be used to improve the safe bio-preservation of foods and therapy of bovine mastitis, and extra cheese whey produced by cheese making industry is reused in the cultivation for probiotics effectively. 相似文献
72.
Iron derivatives from casein hydrolysates as a potential source in the treatment of iron deficiency 总被引:1,自引:0,他引:1
Chaud MV Izumi C Nahaal Z Shuhama T Bianchi Mde L de Freitas O 《Journal of agricultural and food chemistry》2002,50(4):871-877
The properties of an Fe(3+)-peptide complex containing 5.6% Fe, obtained by the reaction of ferric chloride with an enzymatic hydrolysate of casein, are described. The major site of iron binding corresponds primarily to the carboxylate groups and to a lesser extent to the peptide bonds. The Fe(3+)-peptide complex is insoluble at acid pH and completely soluble at neutral to alkaline pH. When soluble, the Fe(3+) is tightly bound to the complex peptide mixture but can be displaced and complexed by a low molecular weight ligand such as cysteine. Its efficacy in relation to iron sulfate was compared in rats. Both iron sources were administrated in Milli-Q water by gastric gavage to male Wistar rats (180-200 g) after an 18 h fast with water ad libitum. Fe(3+) from the Fe(3+)-peptide complex was transferred to the blood in a dose-dependent manner (1-8 mg of Fe/kg), and the serum iron levels were significantly higher (p < 0.001) than in a similar group of rats treated with iron sulfate. In the comparative kinetics experiments, the rats received 4 mg of Fe/kg. Both iron sources presented maximum absorption, as indicated by the elevation of serum iron levels, 30 min after administration, and the AUC(0)(-->2h) of the Fe(3+)-peptide complex was significantly higher (p < 0.05) than that observed with iron sulfate. The simultaneous administration of free peptides (0-192 mg) with the Fe(3+)-peptide complex or iron sulfate did not modify the extent of absorption of iron from both sources, suggesting that the absorption is due to the complex formed and probably not to exchange reactions in the gastrointestinal tract. In the hemoglobin repletion experiments carried out on newly weaned rats with anemia induced by a low-iron diet, supplementation of the diet with the the Fe(3+)-peptide complex was as efficient as supplementation with iron sulfate in the conversion from diet to hemoglobin iron. These results, taken together, suggest that the Fe(3+)-peptide complex is a potential compound for use as an iron source in biological situations. 相似文献
73.
Kojiro Kawada Yasuyuki Sasaki Tadao Asami Shunsuke Yajima Shinsaku Ito 《Journal of Pesticide Science》2022,47(1):43
Strigolactones (SLs) are carotenoid-derived plant hormones involved in several growth and developmental processes. Also, SLs are allelochemicals that induce the seed germination of root parasitic plants and the hyphal branching of arbuscular mycorrhizal fungi. In this study, to identify novel lead chemicals that inhibit SL biosynthesis, we evaluated the effect of agrochemicals on SL biosynthesis. We found that the diacylhydrazine insect growth regulator, chromafenozide, reduced the endogenous level of 4-deoxyorobanchol (4DO), a major SL in rice. Furthermore, treatment with the same class of insect growth regulator, methoxyfenozide, also resulted in the reduction of 4DO levels in rice root exudates. These results suggest that chromafenozide and methoxyfenozide are novel lead inhibitors of SL biosynthesis. 相似文献
74.
Mase M Imada T Nakamura K Tanimura N Imai K Tsukamoto K Yamaguchi S 《Avian diseases》2005,49(4):582-584
We examined the pathogenicity for chickens of two H5N1 avian influenza viruses isolated in Japan, A/chicken/ Yamaguchi/7/2004 (Ck/Yamaguchi/7/04) isolated from outbreaks in commercial layer chickens, and A/duck/Yokohama/aq10/ 2003 (Dk/Yokohama/aq10/03) isolated from duck meat imported from China. All chickens inoculated intranasally with either strain died, and the viruses were reisolated from all organs examined. However, both the mean time of onset of clinical signs and the mean death time of Ck/Yamaguchi/7/04 were shorter than those of Dk/Yokohama/aq10/03. 相似文献
75.
76.
Yumi Umeda Tatsuya Kasai Misae Saito Hitomi Kondo Tadao Toya Shigetoshi Aiso Hirokazu Okuda Tomoshi Nishizawa Shoji Fukushima 《Journal of toxicologic pathology》2013,26(2):131-140
To evaluate pulmonary toxicity of multi-walled carbon nanotubes (MWCNTs), F344 rats of
both sexes were exposed by inhalation to 0.2, 1 or 5 mg/m3 MWCNT aerosol for 6
h/day, 5 days/week for 2 weeks using a whole-body exposure system. At the end of the
2-week exposure period, one-half of the rats were necropsied, and at the end of an
additional 4-week postexposure period, the remaining rats were necropsied. MWCNTs were
deposited in the lungs of all MWCNT-exposed groups and mostly remained in the lungs
throughout the 4-week postexposure period. Granulomatous changes in the lung were found in
the rats exposed to 5 mg/m3 MWCNTs, and these changes were slightly aggravated
at the end of the 4-week postexposure period. In the bronchoalveolar lavage fluid (BALF),
the numbers of neutrophils, percentages of bi- and multinucleated alveolar macrophages,
levels of ALP activity and concentrations of total protein and albumin were elevated in
the rats exposed to 1 and 5 mg/m3 MWCNTs. At the end of the 4-week postexposure
period, the values of the BALF parameters tended to remain elevated. In addition, goblet
cell hyperplasias in the nasal cavity and nasopharynx were observed in the rats exposed to
1 and 5 mg/m3 MWCNTs, but these lesions had largely regressed by the end of the
postexposure period. Based on the histopathological and inflammatory changes, the
no-observed-adverse-effect level (NOAEL) for inhalation of MWCNTs for 2 weeks was 0.2
mg/m3. 相似文献
77.
Hosoya S Villena J Shimazu T Tohno M Fujie H Chiba E Shimosato T Aso H Suda Y Kawai Y Saito T Alvarez S Ikegami S Itoh H Kitazawa H 《Veterinary research》2011,42(1):111
ABSTRACT: This study analyzed the functional expression of TLR3 in various gastrointestinal tissues from adult swine and shows that TLR3 is expressed preferentially in intestinal epithelial cells (IEC), CD172a+CD11R1high and CD4+ cells from ileal Peyer's patches. We characterized the inflammatory immune response triggered by TLR3 activation in a clonal porcine intestinal epitheliocyte cell line (PIE cells) and in PIE-immune cell co-cultures, and demonstrated that these systems are valuable tools to study in vitro the immune response triggered by TLR3 on IEC and the interaction between IEC and immune cells. In addition, we selected an immunobiotic lactic acid bacteria strain, Lactobacillus casei MEP221106, able to beneficially regulate the anti-viral immune response triggered by poly(I:C) stimulation in PIE cells. Moreover, we deepened our understanding of the possible mechanisms of immunobiotic action by demonstrating that L. casei MEP221106 modulates the interaction between IEC and immune cells during the generation of a TLR3-mediated immune response. 相似文献
78.
Hirosuke Oba Naoto Shinozaki Hiroshi Oyaizu Keitaro Tawaraya Tadao Wagatsuma Wilfredo L. Barraquio 《Soil Science and Plant Nutrition》2013,59(8):1195-1203
An extensive area has been buried due to the repeated occurrence of mud flows (lahars) derived from volcanic deposits during the eruption of Mt. Pinatubo in the Philippines. Most of the area was covered with sparse vegetation consisting of only a few gramineous pioneer plants such as Saccharum spontaneum (site SV). However dense vegetation consisting of wild leguminous plants such as Calpogonium mucunoides showed a patch distribution (site DV). In 1999, we investigated the community composition of AMF at these two sites. S. spontaneum at both sites was slightly colonized with AMF while the leguminous plants were highly colonized. Spores of AMF were collected from the rhizosphere of these plants. Eight spore morphotypes were identified; one each for Acaulospora and Entrophospora colombiana, two for Glomus, one for Paraglomus, and three for Scutellospora. Part of the 18S rRNA gene of AMF colonizing the plant roots was amplified with AMF-specific primers, NS31 and AM1, cloned and sequenced. Fifty-three AMF clones were phylogenetically classified into 8 phylotypes as follows: one each for Acaulospora and E. colombiana, five for Glomus, and one for Scutellospora. Both molecular and morphological examinations showed that the diversity of AMF was comparable to that in other temperate ecosystems with abundant vegetation and did not differ significantly between sites SV and DV, regardless of the vegetation cover. Furthermore, S. spontaneum supported diverse AMF species in spite of its scant growth at site SV. E. colombiana was mostly associated with C. mucunoides. Significance of AMF for the primary plant succession in the lahar area was analyzed. 相似文献
79.
Tanimura N Imada T Kashiwazaki Y Shamusudin S Syed Hassan S Jamaluddin A Russell G White J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(10):1263-1266
The immunohistochemical reactivity of seven clones of mouse monoclonal antibodies raised to Nipah virus antigens were investigated using formalin-fixed, paraffin embedded porcine and equine lung tissues from experimental Nipah and Hendra virus infection, respectively. Either microwave irradiation or enzymatic digestion effectively unmasked the viral antigens in formalin-fixed, paraffin-embedded tissue sections. Four clones showed positive reaction to both Nipah virus-infected porcine lung tissue and Hendra virus-infected equine lung tissue. Two clones (11F6 and 13A5) reacted with Nipah virus-infected porcine lung tissue, but not with Hendra virus-infected equine lung tissue. These Nipah virus-specific monoclonal antibodies may therefore be useful for immunohistological diagnosis of Nipah virus infection and for further research on Nipah virus pathogenesis. 相似文献
80.
Advanced molecular immunoassay system for immunobiotic lactic acid bacteria using a transfectant of Toll-like receptor 2 总被引:1,自引:1,他引:1
Masanori TOHNO Takeshi SHIMOSATO Yasushi KAWAI Hisashi ASO Shuji IKEGAMI Naoki TAKETOMO Tadao SAITO Haruki KITAZAWA 《Animal Science Journal》2007,78(2):195-205
Toll‐like receptor 2 (TLR2) is a receptor for a variety of microbial components, and it also mediates activation signals in the cell relating to the innate immune system. In order to evaluate the precise molecular immunoregulation by various strains of lactic acid bacteria (LAB) via TLR2, the swine TLR2 (sTLR2)‐expressing transfectant was constructed using human embryonic kidney (HEK) 293 cells. It is demonstrated that intact immunobiotic LAB can induce immune responses through TLR2, and that different nuclear factor‐κB (NF‐κB) activities of various strains can be accurately detected by sTLR2‐expressing HEK293 cells. Furthermore, cellular activation of NF‐κB via TLR2 is reflected in enhanced binding and uptake of LAB. The sTLR2‐expressing HEK293 cells were also useful for characterizing the expression pattern of type I helper T (Th1) and type II helper T (Th2) cytokines by the stimulation of immunobiotic LAB. These results suggest that sTLR2‐expressing HEK293 cells may be useful in certain molecular immunoassay systems for producing new physiologically functional foods with intestinal immunomodulatory abilities, such as the maintenance of Th1/Th2 polarization. 相似文献