全文获取类型
收费全文 | 521篇 |
免费 | 3篇 |
专业分类
林业 | 19篇 |
农学 | 3篇 |
53篇 | |
综合类 | 132篇 |
农作物 | 11篇 |
水产渔业 | 5篇 |
畜牧兽医 | 295篇 |
园艺 | 1篇 |
植物保护 | 5篇 |
出版年
2012年 | 24篇 |
2011年 | 44篇 |
2010年 | 7篇 |
2008年 | 31篇 |
2007年 | 56篇 |
2006年 | 46篇 |
2005年 | 48篇 |
2004年 | 40篇 |
2003年 | 26篇 |
2002年 | 21篇 |
2001年 | 23篇 |
2000年 | 38篇 |
1999年 | 11篇 |
1997年 | 2篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1987年 | 4篇 |
1980年 | 4篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1972年 | 2篇 |
1963年 | 2篇 |
1959年 | 2篇 |
1958年 | 3篇 |
1956年 | 2篇 |
1948年 | 3篇 |
1940年 | 2篇 |
1922年 | 1篇 |
1921年 | 1篇 |
1918年 | 1篇 |
1906年 | 1篇 |
1905年 | 1篇 |
1903年 | 1篇 |
1899年 | 2篇 |
1897年 | 2篇 |
1896年 | 4篇 |
1895年 | 2篇 |
1893年 | 2篇 |
1886年 | 1篇 |
1885年 | 2篇 |
1883年 | 3篇 |
1881年 | 1篇 |
1880年 | 2篇 |
排序方式: 共有524条查询结果,搜索用时 734 毫秒
71.
72.
Cheng IC Liang SM Tu WJ Chen CM Lai SY Cheng YC Lee F Huang TS Jong MH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(8):859-864
Monoclonal antibodies (MAbs) reported here were produced against the porcinophilic foot-and-mouth disease virus (FMDV) that caused the devastating swine disease on 1997 in Taiwan. A panel (25) of MAbs were found to react with VP1 of O/Taiwan/97 (O/97) by ELISA with various potencies. The biological identities of these VP1 reacting MAbs, such as neutralization activity, isotype and capability to distinguish between two serotype O FMDVs, O/97 and O/Taiwan/KM1/99 (O/99), were further analyzed. Eleven out of the total eighteen O/97 neutralizing MAbs were able to neutralize heterologous O/99. Eight O/97 neutralizing and five non-neutralizing MAbs could differentiate two serotype O FMDVs by immunofluorescence assay (IFA) implied that these thirteen MAbs recognized O/97 specific epitope(s). Furthermore, reactivities of the VP1 reacting MAbs with a 29 amino acids synthetic peptide (P29) representing the betaG-betaH loop of VP1 were analyzed by ELISA and fourteen were found positive. MAb clone Q10E-3 reacting strongest with VP1 and P29, neutralizing both but not differentiating two serotype O viruses suggested that the antibody binding site might involve the RGD motif and its C terminal conserved region on betaG-betaH loop. MAbs with diverse characters presented in this study were the first raised against porcinophilic FMDV. The complete set of MAbs may be used for further studies of vaccine, diagnostic methods, prophylaxis, etiological and immunological researches on FMDV. 相似文献
73.
Adamama-Moraitou KK Brellou GD Rallis TS Zavros N Pardali D Dinopoulos A Vlemmas I 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2006,53(2):81-84
A 3.5-year-old intact male American Pit Bull was presented because of urinary incontinence and dysuria. Constipation, followed by diarrhoea, ocular disturbances and finally regurgitation developed over the next 4 years. Autonomic dysfunction was evidenced by clinical presentation, as well as positive ophthalmic pilocarpine test and subnormal Schirmer tear test. Diagnosis, however, was established through histopathological and immunohistochemical examination. Lesions attributable to inflammatory degenerative neuropathy of the autonomic ganglia, which represents one of the various types of human autonomic failure, were detected. 相似文献
74.
Floto RA MacAry PA Boname JM Mien TS Kampmann B Hair JR Huey OS Houben EN Pieters J Day C Oehlmann W Singh M Smith KG Lehner PJ 《Science (New York, N.Y.)》2006,314(5798):454-458
An effective host immune response to mycobacterial infection must control pathogen dissemination without inducing immunopathology. Constitutive overexpression of mycobacterial heat shock protein (myHsp70) is associated with impaired bacterial persistence, but the immune-mediated mechanisms are unknown. We found that myHsp70, in addition to enhancing antigen delivery to human dendritic cells, signaled through the CCR5 chemokine receptor, promoting dendritic cell aggregation, immune synapse formation between dendritic cells and T cells, and the generation of effector immune responses. Thus, CCR5 acts as a pattern-recognition receptor for myHsp70, which may have implications for both the pathophysiology of tuberculosis and the use of myHsps in tumor-directed immunotherapy. 相似文献
75.
Zwier TS 《Science (New York, N.Y.)》2012,335(6069):668-669
76.
Radiative absorption enhancements due to the mixing state of atmospheric black carbon 总被引:3,自引:0,他引:3
CD Cappa TB Onasch P Massoli DR Worsnop TS Bates ES Cross P Davidovits J Hakala KL Hayden BT Jobson KR Kolesar DA Lack BM Lerner SM Li D Mellon I Nuaaman JS Olfert T Petäjä PK Quinn C Song R Subramanian EJ Williams RA Zaveri 《Science (New York, N.Y.)》2012,337(6098):1078-1081
Atmospheric black carbon (BC) warms Earth's climate, and its reduction has been targeted for near-term climate change mitigation. Models that include forcing by BC assume internal mixing with non-BC aerosol components that enhance BC absorption, often by a factor of ~2; such model estimates have yet to be clearly validated through atmospheric observations. Here, direct in situ measurements of BC absorption enhancements (E(abs)) and mixing state are reported for two California regions. The observed E(abs) is small-6% on average at 532 nm-and increases weakly with photochemical aging. The E(abs) is less than predicted from observationally constrained theoretical calculations, suggesting that many climate models may overestimate warming by BC. These ambient observations stand in contrast to laboratory measurements that show substantial E(abs) for BC are possible. 相似文献
77.
78.
Induction of feline immunodeficiency virus from a chronically infected feline T-lymphocyte cell line
The infection of the feline T-lymphocyte cell line FeT-J with the feline immunodeficiency virus (FIV) Petaluma strain led to the establishment of nonvirus-producing cells. One clone (C15) obtained by limiting dilution was found to express FIV in response to chemical inducers of retroviruses. The chemical treatment of C15 cells led to not only FIV protein synthesis but also an augmentation of viral production. Examination of the C15 cell derivatives obtained by recloning revealed that 10-40% of treated cells constitutively expressed FIV antigens, whereas 100% with expressed FIV antigen in response to the inducer. Chemical induction resulted in more than a 100-fold increase in infectious viral production. The results suggest that a majority of FeT-J cells that are infected with FIV exist in a non-productive state. Establishing a cell line that can be non-productively infected by FIV may help determine the mechanisms of FIV latency. 相似文献
79.
Baccarin RY Machado TS Lopes-Moraes AP Vieira FA Michelacci YM 《Research in veterinary science》2012,93(1):88-96
Our objectives were to characterize the urinary excretion of glycosaminoglycans (GAGs) in horse osteoarthritis, and to investigate the effects of chondroitin sulfate (CS) and glucosamine (GlcN) upon the disease. Urinary GAGs were measured in 47 athletic horses, 20 healthy and 27 with osteoarthritis. The effects of CS and GlcN were investigated in mild osteoarthritis. In comparison to normal, urinary GAGs were increased in osteoarthritis, including mild osteoarthritis affecting only one joint. Treatment with CS+GlcN led to a long lasting increase in the urinary CS and keratan sulfate (KS), and significant improvement in flexion test of tarsocrural and metacarpophalangeal joints was observed. In conclusion, urinary CS and KS seems to reflect the turnover rates of cartilage matrix proteoglycans, and the measurement of these compounds could provide objective means of evaluating and monitoring joint diseases. 相似文献
80.
Adamama-Moraitou KK Pardali D Day MJ Prassinos NN Kritsepi-Konstantinou M Patsikas MN Rallis TS 《Veterinary journal (London, England : 1997)》2012,191(2):261-266
Canine bronchomalacia (BM) is characterized by weakness leading to collapse of the bronchial wall. A prospective study of 18 affected dogs (age range: 1-15 years) was undertaken to characterize the clinicopathological and histological features of BM. Poodles and Yorkshire terriers were commonly affected. Half of the dogs were overweight or obese. The clinical presentation was a mild, wheezing, chronic cough and pulmonary crackles were heard in 28% of the dogs. Compatible radiographic changes were present in 61% of the dogs. Using bronchoscopy, both lungs were affected in half of the animals, whereas in the others the disease appeared to affect predominantly the left lung. Analysis of bronchoalveolar lavage fluid and biopsies of bronchial mucosa revealed pure or mixed neutrophilic inflammation. Underlying infectious bronchitis was considered possible in 56% of the dogs. It was concluded that canine BM may present as an isolated clinical entity associated with infection and/or inflammation. 相似文献