基于路网的石羊河流域乡镇空间测度

道路交通的通达性是城市空间相互作用和经济联系的重要前提。本研究利用ArcGIS的网络分析和空间分析功能在对石羊河流域公路交通网络通达性定量评价的基础上,采用引力、潜力和场强模型对区域内7个县（区）的空间相互作用程度、城市潜力值和城市的辐射场强进行定量测度和比较分析。研究发现,凉州区对区域内城市作用力最强,城市对外相互作用潜力大,辐射带动作用强度高。区域整体上形成了以金川区、凉州区为中心的辐射中心,其对区域内其他县、区的辐射强度随交通距离增加和道路通达性降低而不断减小。     

Fermentation quality and chemical composition of shrub silage treated with lactic acid bacteria inoculants and cellulase additives

Effects of lactic acid bacteria (LAB) inoculants and cellulase additives on fermentation quality and chemical compositions of shrub silages were studied by using a small‐scale fermentation system. Two LAB inoculants of Qingbao (Lactobacillus plantarum, Pediococcus acidilacticii, Lactobacillus casei and Clostridium phage) and Caihe (Lactobacillus plantarum, Lactobacillus brevis and Pediococcus acidilactici) and a commercial cellulase made from Trichoderma reesei were used as additives for intermediate pea‐shrub, rush bushclover, arborescent ceratoides and shrubby silage preparation. The crude protein, neutral detergent fiber and water‐soluble carbohydrate contents of the four shrub materials were 10.1–14.2, 62.6–67.2 and 1.9–3.5% on a dry matter basis, respectively. All shrub silages had pH 3.40–4.43, ammonia‐N 0.1–0.2% g/kg and lactic acid 1.3–2.9% on a fresh matter basis. The silage quality of LAB‐inoculated silages did not have a greater effect than control silages, except shrubby silage preparation. Silages treated with the cellulase, the pH of rush bushclover and shrubby sweetvetch silage were significantly (P < 0.05) lower and the lactic acid content were significantly (P < 0.05) higher than the control silages. The results confirmed that shrub contained a relatively high content of crude protein; its silages can be preserved in good quality, and they are new potential resources for livestock feed.     

Simultaneous Infection of Pigs and People with Triple‐Reassortant Swine Influenza Virus H1N1 at a U.S. County Fair

Influenza‐like illness was noted in people and pigs in attendance at an Ohio county fair in August 2007. The morbidity rate in swine approached 100% within 1–2 days of initial clinical signs being recognized, and approximately two dozen people developed influenza‐like illness. Triple‐reassortant swine H1N1 influenza viruses were identified in both pigs and people at the fair. The identified viruses (A/Sw/OH/511445/2007, A/Ohio/01/2007, and A/Ohio/02/2007) were similar to H1N1 swine influenza viruses currently found in the U.S. swine population. This case illustrates the possibility of transmission of swine influenza in settings where there is close human/swine interaction.     

胚培杏新品种———山农凯新1号

 ‘山农凯新1号’是以丰产性强的‘凯特’杏为母本, 以品质优良的‘新世纪’杏为父本进行有性杂交, 结合胚培技术育成的新品种, 具有成熟早、香味浓、品质优、自交坐果率高、丰产性强等特点。     

Antisense c-myc sensitizes osteosarcoma cells to cisplatin-induced apoptosis

AIM: To down-regulate expression of c-myc through antisense therapy and to investigate its effect on the sensitivity of osteosarcoma MG-63 cells to cisplatin-induced apoptosis. METHODS: The recombinant adenovirus (Ad-Asc-myc) encoding antisense c-myc fragment was constructed and transfected into osteosarcoma MG-63 cells in vitro in order to down-regulate the expression of c-myc, and the change in the sensitivity to cisplatin-induced apoptosis was observed. MTT, Western blot, RT-PCR, flow cytometry (FCM) and electron microscope were used to evaluate tumor cell proliferation in vitro, genes expression related to apoptosis regulation and effects on the sensitivity of osteosarcoma MG-63 cells to cisplatin-induced apoptosis. RESULTS: Ad-Asc-myc down-regulated the expression of c-myc protein after transfected MG-63 cells for 48 h, combined with the treatment of 2.0 mg/L cisplatin for 2 h inhibited tumor cell proliferation in vitro by 38.0%. RT-PCR revealed that Ad-Asc-myc down-regulated the expression of Bcl-2 and up-regulated the expression of Bax. No appreciable change was observed in the expression of E2F-1. FCM showed that Ad-Asc-myc induced apoptosis in intransfected cells, and rendered it more sensitive to cisplatin. CONCLUSION: Antisense c-myc is able per se to induce apoptosis and sensitize osteosarcoma cells to cisplatin-induced apoptosis.     

优质晚熟葡萄新品种‘钟山红’

 ‘钟山红’葡萄是从‘魏可’实生系中选育而成的晚熟新品种。树势强旺,成花容易,晚果丰产,果实挂树时间长,耐贮运,抗病性中等,适应性强。果穗圆锥形,平均单穗质量600 g,果粒为长椭圆形,平均单粒质量9.53 g,果皮紫黑色,果肉致密硬脆,味甜爽口,风味独特,品质上等,可溶性固形物含量22% ~ 23%,最高达25%,酸含量0.44% ~ 0.65%,果实完全成熟期在9月中下旬。     

Enhancement effect of adenovirus mediated antisense c-myc gene and caffeine on chemotherapy of osteosarcoma cells to cisplatin

AIM: The cancer biology has showed that overexpression of oncogenes is responsible for the progression of human malignancies,antisense technology can block a certain gene expression.Caffeine has enhancement effect on chemotherapy of osteosarcoma cells to cisplatin,we constructed the recombinant adenovirus (Ad-Asc-myc) encoding antisense c-myc fragment and investigated its effect on the in vitro sensitivity of osteosarcoma MG-63 cells to cisplatin.METHODS: The recombinant adenovirus (Ad-Asc-myc) encoding antisense c-myc fragment was constructed by cloning c-myc cDNA of about 750 base pairs in a reverse direction into adenovirus vector.Ad-Asc-myc and caffeine was used respectively or together to co-operate with cisplatin to treat the osteosarcoma MG-63 cells in vitro,and Western blotting,MTT,flow cytometry (FCM),electron microscope were used to evaluate expression of c-Myc protein,tumor cell proliferation in vitro,apoptosis and cell cycle analysis.RESULTS: Ad-Asc-myc was obtained with the titer of 2×10¹² pfu/L.Ad-Asc-myc down-regulated the expression of c-Myc protein,Ad-Asc-myc or caffeine enhanced the effects of 2.0,5.0 mg/L cisplatin on MG-63 cells.Moreover,Ad-Asc-myc combined with caffeine significantly enhanced this effects,not only on cisplatin-induced apoptosis,but also on tumor cells proliferation in vitro.The expression of bcl-2 was downregulated,bax were upregulated,while there was no change in the expression of E2F-1.FCM analysis showed that cisplatin treatment induced a block in S phase,and caffeine reversed this block and speeded up the progression of cells out of the S phase.Ad-Asc-myc induced obvious G₂/M phase arrest in transfected cells.CONCLUSION: Ad-Asc-myc combined with caffeine may enhance apoptosis-induced and chemotherapy effects of osteosarcoma MG-63 cells to cisplatin.     

煤矸石栽培基质在农业中资源化利用研究现状

随着煤矸石在农业方面研究的不断深入,其作为栽培基质的开发和应用已成为重要的研究领域.该研究在梳理和分析国内外研究成果的基础上,综述了煤矸石理化及生物学特性、利用途径、已取得的主要成果及存在的问题,指出今后煤矸石在农业资源化利用中的发展趋势.     

温度和光照对金边瑞香花芽分化的影响

研究了温度和光照对金边瑞香花芽分化的影响,温度是影响金边瑞香花芽分化的主要因子,20 ~ 25 ℃金边瑞香植株可以完成花芽分化的各个过程,10 ~ 18 ℃植株一直处于营养生长状态而未进行花芽分化。金边瑞香花芽分化对光周期不敏感,但散射光比直射光更有利于其花芽分化。     

‘南果梨’采后衰老机理与保鲜技术研究进展

该文对‘南果梨’贮藏(20℃、0℃)过程中酶褐变进行了研究,同时研究了真空浸钙和MA贮藏(薄膜小包装自发式气调贮藏)对‘南果梨’褐变的影响。研究结果表明,20℃下‘南果梨’果心和果肉有很高的PPO(多酚氧化酶)活性,PPO同功酶在贮藏中后期酶带增多。‘南果梨’的褐变随贮期延长而增加,主要为衰老褐变。