一株牦牛源产细菌素植物乳杆菌SWUN5815全基因组测序及序列分析

旨在分析一株牦牛源植物乳杆菌SWUN5815的全基因组序列测序,并对该菌株的功能特性基因进行挖掘.基于PacBio RSII测序平台对乳杆菌SWUN5815进行全基因组测序分析和GO、KEGG、COG和NR数据库进行基因组基本功能注释.结果 表明:1)植物乳杆菌SWUN5815的基因组大小为3.27 Mb,GC含量44...     

保护性耕作食用向日葵田间杂草防除试验结果

食用向日葵田间杂草防除试验结果表明,在保护性耕作条件下施用化学除草剂防除效果不如人工除草 传统耕作,但由于保护性耕作提高了土壤的保肥保墒作用,降低了用工成本,向日葵产量和产值比人工除草 传统耕作分别提高了12.34%、39.54%.     

大豆打顶试验结果初报

2005年进行的冀黄12和中黄19两个大豆品种的打顶试验结果表明, 冀黄12在苗高27.5 cm、中黄19在苗高30.0 cm时打顶4 cm,大豆经济性状表现最好,生育期可缩短3～4 d,单株结荚数增加11.30～11.58个,单株粒数增加31.04～36.73粒,折合产量最高,分别为3 683.33、3 800.00 kg/hm2,分别较CK1、CK2(不打顶)增产38.4%、40.3%,且与CK1、CK2产量差异达极显著水平.打顶过长(6 cm)会减少单株结荚数、单株粒数和单产.     

一种简单、有效的提取根瘤菌总RNA的方法

介绍了一种适合于根瘤菌及其它革兰氏阴性细菌的RNA提取方法。它具有简便、快速等特点,且所提取的RNA样品质量较高,可直接用于RT-PCR合成、Northern杂交等分子生物学操作。该方法具有实用性强、重复性好的特点。提取的RNA无DNA等污染物,并且其产量、纯度完全能满足分子克隆和基因表达研究的需要。该方法简单、快速、纯度高、完整性强,方便有效。     

Evaluation of the Pathotypes of Xanthomonas oryzae pv. oryzae and Preliminary Analysis of the Resistant Reactions of Main Japonica Rice in the Yunnan Plateau, China

The pathogenicity of 36 isolates of Xanthomonas oryzae pv. oryzae （Xoo）, which were collected from japonica rice varieties in the Yunnan Plateau, China, was evaluated. It was evaluated on 29 rice varieties including a set of seven varieties to identify pathogenicity, i.e., Haonuoyang, TN1, Kogyoku, Zhenzhu＇ai, IR26, Nanjing 33, and Kinmaze, which may be considered as a set of differential varieties for Xoo races from Yunnan japonica rice. The efficiency of the seven varieties was further confirmed. The results showed reversible and specific interactions between isolates and varieties. The isolates were classified into nine pathotypes from pathotyp Ⅰ to Ⅸ according to their pathogenic reactions on the seven rice varieties. The pathotype V was the epidemic, whereas pathogen Ⅶ was the most pathogenic. Most japonica varieties grown in the Yunnan Plateau were susceptible to Xoo. The rice lines IRBB21 （Xa-21）, Zhachanglong （Xa-22,, Xa- 24,）, and IR1545-339 （xa-5）, which were resistant to all the isolates tested, can be used as donors of resistant genes for bacterial blight in japonica rice breeding in the Yunnan Plateau.     

The Effect of Tryptophan on Serotonin-Like Neurons in Duck Cerebellum

Healthy Cherry Valley ducks were used in the present study. Different doses of tryptophan were injected intraperitoneally to them after being fasted 4 h （8：00 a.m.-12：00 a.m.）. One hour later, they were deeply anaesthetized and perfused. The cerebellum was removed to make serial paraffin longitudinal sections. The streptavidin-biotin-peroxidase complex （SABC） method was used to study the distribution of serotonin-like neurons in the cerebellum. All films were analysed by using a computer-assisted image analysis system. Serotonin-like neurons are only localized in cerebellar Purkinje cell layer. The optical density averages of serotonin-like neurons in 200 and 100 mg kg^-1 group are significantly higher than that of 0 mg kg^-1 group （P〈0.01）. These results show that serotonin-like neurons are distributed in Purkinje cell layer and that excessive tryptophan can affect the content of serotonin in cerebellum.     

纺高支纱陆地棉主要经济性状间相关性分析

利用单相关、偏相关和通径分析等多种相关分析方法,研究了纺高支纱陆地棉主要产量、品质性状间的相关关系。结果表明:各性状对皮棉产量的单相关系数由大到小依次为株铃数、衣分、铃重、衣指、马克隆值、绒长、比强度、籽指;偏相关系数由大到小依次为株铃数、铃重、比强度、衣分、衣指、籽指、绒长、马克隆值;直接通径系数由大到小依次为株铃数、衣分、铃重、衣指、籽指、比强度、马克隆值、绒长。比强度与单株皮棉产量的单相关系数、偏相关系数和直接通径系数分别为-0.206,0.481,0.129。绒长与单株皮棉产量成不显著负相关,与比强度、马克隆值成正相关。马克隆值与比强度成不显著负相关。以上结果说明高产纺高支纱陆地棉首先应具有结铃性强、成铃多的基本性状,同时要兼顾衣分;选择高强纤维不会影响高产基因型的获得;纺高支纱陆地棉存在纤维品质不配套的问题,应进一步改良。文章还对纺高支纱陆地棉的选育提出了可资借鉴的建议。     

五香兔肉加工工艺及配方优化研究

研究了五香兔肉的加工工艺与配方,通过正交试验确定了加工五香兔肉主要调味料的最佳添加量为:4％食盐,2％五香粉,0.04％肉类香精.在此基本配方的基础上,如再分别添加0.4％茴香、0.2％花椒、0.6％～0.8％辣椒或2％～4％白糖,可制作出不同风味的五香兔肉.     

冬种黑麦草对6种水稻土重金属含量及晚稻不同器官重金属累积与分配的影响

研究双季稻区6种不同土壤类型冬季种植黑麦草对土壤重金属含量及其对晚稻不同器官重金属积累与分配的影响, 可为水稻质量安全生产和产地安全适宜性评价提供重要参考。本文以7年定位试验为基础, 研究了南方双季稻区冬季种植黑麦草对6种不同水稻土耕层土壤重金属Cd、Pd、Hg和As含量的变化及其对晚稻不同器官重金属积累与分配的影响。结果表明, 在6种不同土壤类型中, 灰泥田土壤Cd含量极显著高于其他土壤类型; 黄泥田土壤Pd含量极显著高于其他土壤类型; 土壤Hg含量以红黄泥田最高, 达0.029 mg kg^-1, 与其他土壤类型差异达极显著水平; 土壤As的含量以黄泥田最高, 为29.5 mg kg^-1, 极显著高于其他土壤类型。6种不同土壤类型晚稻植株Cd、Pb、As含量均为根>茎叶>糙米。在晚稻植株根系、茎叶和糙米的Cd含量以红黄泥田最高, 均极显著高于其他土壤类型; 植株根系Pb、As含量以黄泥田最高, 分别为33.0 mg kg^-1和119.5 mg kg^-1, 均极显著高于其他土壤类型; 河沙泥水稻植株茎叶的Hg、As含量均极显著高于其他土壤类型。说明黄泥田种植水稻有利于降低稻米中重金属含量, 改善稻米品质。     

Thermal Performance and Energy Analysis of Green Roof

Energy consumption and thermal parameters of green roof and bare roof were measured in the air conditioned and nature indoor environment in summer.It was found that the heat flow of green roof was reduced about 70%, and additional equivalent thermal resistance of green roof was about 1.0 m2·K/W in the air conditioned,cooling efficiency of green roof was about 111% in the nature condition.