全文获取类型
收费全文 | 7840篇 |
免费 | 590篇 |
国内免费 | 632篇 |
专业分类
林业 | 686篇 |
农学 | 400篇 |
基础科学 | 321篇 |
718篇 | |
综合类 | 3949篇 |
农作物 | 654篇 |
水产渔业 | 355篇 |
畜牧兽医 | 1043篇 |
园艺 | 640篇 |
植物保护 | 296篇 |
出版年
2024年 | 65篇 |
2023年 | 162篇 |
2022年 | 332篇 |
2021年 | 313篇 |
2020年 | 346篇 |
2019年 | 366篇 |
2018年 | 244篇 |
2017年 | 436篇 |
2016年 | 241篇 |
2015年 | 370篇 |
2014年 | 371篇 |
2013年 | 478篇 |
2012年 | 653篇 |
2011年 | 669篇 |
2010年 | 652篇 |
2009年 | 612篇 |
2008年 | 612篇 |
2007年 | 526篇 |
2006年 | 490篇 |
2005年 | 344篇 |
2004年 | 194篇 |
2003年 | 131篇 |
2002年 | 128篇 |
2001年 | 138篇 |
2000年 | 124篇 |
1999年 | 30篇 |
1998年 | 5篇 |
1995年 | 3篇 |
1994年 | 5篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 1篇 |
1987年 | 1篇 |
1966年 | 2篇 |
1963年 | 3篇 |
1962年 | 1篇 |
1958年 | 1篇 |
1956年 | 6篇 |
排序方式: 共有9062条查询结果,搜索用时 46 毫秒
991.
分别采用苯酚硫酸法、高效液相色谱法(HPLC)测定了野生戴氏虫草的多糖、腺苷、甘露醇的含量;利用NIH小鼠进行碳廓清试验、免疫器官重量和迟发型超敏反应试验,通过急性毒性试验确定戴氏虫草水提液小鼠LD50。试验结果表明,野生戴氏虫草的多糖、腺苷及甘露醇含量分别为36.20mg/g、0.27mg/g和80.20mg/g;戴氏虫草水提液能提高非特异性免疫功能,提高受环磷酰胺抑制的小鼠免疫器官重量和细胞免疫功能,其小鼠经口LD50〉69.42g/kg。 相似文献
992.
自1998年始,我们已在湖南、江苏等地建立了65ha以上红汁乳菇马尾松种植园。这些林分一般三年后开始产菇,年产量可达675kg/ha。为了确认所产红汁乳菇子实体来源于接种的菌根苗而非自然侵入的孢子,我们进行了子实体、菌根苗菌根的分子研究。首次比较了试验地所产子实体与红汁乳菇-马尾松菌根苗培养钵内的菌根的ITS序列。同时,用RAPD法分析了取自于试验地内的10个红汁乳菇子实体。结果表明,10个子实体中的9个与起始菌种CX1相比具有75%~96%的相似性。其余一个及作为外群取自于340km外的广西融水的子实体与起始菌种分别具有61%和37%的相似性。我们认为,上述9个子实体与起始菌种的相关性比外群和第10个子实体更为紧密。推测认为起始菌株及其子代菌丝形成了上述9个子实体,而其余一个子实体可能来源于周边森林侵入的孢子。 相似文献
993.
994.
995.
TAN Qiu-chan CHEN Zhan-ru YU Mei-sheng LIANG Xie-chou ZHAO chan GAO Hong ZHENG Yan-fang WU Jia-bao ZHU Lin-yan WANG Li-wei CHEN Li-xin 《园艺学报》2018,34(2):335-339
AIM: To study the effect of ClC-3 gene over-expression on thyroid structure and function in mice.METHODS: Three-months-old FVB mice were used to study the difference of thyroid structure and function between wild-type (WT) mouse and ClC-3 transgene mice. The expression and distribution of ClC-3 in the thyroid of mice were determined by the methods of qPCR, Western blot and immunofluorescence. Behavioral monitoring was performed on the daily activities of mice. Serum concentrations of total triiodothyronine (TT3), total thyroxine (TT4) and thyrotropin (TSH) were measured by ELISA.RESULTS: Compared with the WT group, the expression of ClC-3 in the thyroid of ClC-3 transgene group was significantly increased (P<0.05). The thyroid gland showed obvious hyperplasia and the folliculi glandulae thyreoideae was significantly bigger in ClC-3 transgene mice (P<0.05). The weight loss was increased in ClC-3 transgene mice (P<0.05). The expression of TT3 and TT4 were significantly higher than that of WT group (P<0.05), but the change of TSH was not obvious.CONCLUSION: ClC-3 over-expression results in thyroid hyperplasia and thyroid hormone secretion. This study suggests that ClC-3 is likely to be involved in the synthesis of thyroid hormones. 相似文献
996.
TAN Shu-fang ZHANG Li-zhi HUANG Lin-jie CHEN Ming ZHANG Wei SHI Jian-ting TANG Tian-tian LIN Xiao-ling 《园艺学报》2018,34(8):1383-1389
AIM:To investigate the relationship between blood eosinophil count and risk of postoperative pneumonia in patients with coronary artery bypass graft (CABG) surgery. METHODS: The medical records of 613 patients who underwent CABG surgery from January 2008 to December 2017 in our hospital were collected and reviewed. The incidence of postoperative pneumonia and hospital mortality were compared in the patients with low or high blood eosinophil count. The risk factors for postoperative pneumonia following CABG surgery were analyzed by multiple logistic regression. RESULTS: The patients enrolled in the study were 582, in which 220 patients had preoperative blood eosinophil counts of less than 2% (low blood eosinophil count) and 362 patients had eosinophil counts of 2% or more (high blood eosinophil count). The incidence of postoperative pneumonia in the patients with low blood eosinophil count was 14.1% (31/220), significantly higher than that (6.4%, 23/362) in the patients with high blood eosinophil count (P=0.002). However, the hospital mortality of the patients with high or low blood eosinophil count had no difference. Low blood eosinophil count (OR=3.521, 95% CI:1.213~10.223, P=0.021), nasogastric tube (OR=6.490, 95% CI:2.757~15.280, P<0.001) and mechanical ventilation ≥ 24 h (OR=3.496, 95% CI:1.156~10.178, P=0.035) were identified as independent risk factors for postoperative pneumonia following CABG surgery. CONCLUSION: Low blood eosinophil count is associated with increased risk of postoperative pneumonia following CABG surgery. 相似文献
997.
998.
AIM: To screen the differentially expressed long non-coding RNA (lncRNA) in colon cancer, and to explore its expression in colon cancer tissues and adjacent tissues. METHODS: The "Colon adenocarcinoma:Person neoplasm cancer status" which consisted of 36 cases of colon cancer tissues and 29 cases of normal colonic tissues was downloaded from the lncRNAtor database. The candidate genes were selected from these differentially expressed lncRNAs based on artificial criterion (P<0.01; fold change ≥ 2 or<0.5) and then validated by real-time PCR in 60 pairs of colon cancer tissues and adjacent tissues. RESULTS: A total of 50 lncRNAs were differentially expressed in colon cancer tissues, including 28 up-regulated and 22 down-regulated (P<0.01). The verifying results displayed that HNF1A-AS1 and ZDHHC8P1 were up-regulated (P<0.01), and SUZ12P expression was down-regulated (P<0.05), but the expression of AC069513.3 was not statistically significant between colon cancer tissues and adjacent tissues. The abilities of HNF1A-AS1, ZDHHC8P1, SUZ12P and AC069513.3 to discriminate the colon cancer from normal adjacent tissue by the ROC curve with an AUC of 0.729 (sensitivity 78%, specificity 67%), 0.617 (sensitivity 68%, specificity 55%), 0.689 (sensitivity 66%, specificity 55%) and 0.518 (sensitivity 52%, specificity 48%) were observed. CONCLUSION: Long non-coding RNA HNF1A-AS1 and ZDHHC8P1 are up-regulated and SUZ12P is down-regulated in colon cancer tissues, suggesting that they may be involved in the pathogenesis of colon cancer. 相似文献
999.
为研究安格斯牛各体尺指标及体重间的相关关系,掌握其生长发育规律,试验采集了新疆天山某安格斯牛场出生于2012-2016年的安格斯牛生长发育记录,共649条,其中主要包括体重及体尺指标,应用SPSS 16.0软件分析安格斯牛主要体尺指标及体重的相关性。结果发现:安格斯牛体尺指标及体重均随着月龄的增加逐步增大;14月龄之前各体尺指标增长速度较快,14月龄以后增长速度的缓;腹围和胸围增长速度高于其他指标。安格斯公牛的体长指数、体躯指数、胸围指数和管围指数平均分别为111.99%、129.79%、145.26%和16.71%;母牛体长指数、体躯指数、胸围指数和管围指数平均分别为112.15%、132.01%、147.86%和16.95%。安格斯牛体重与体高、体长、胸围、腹围和管围的相关性均达到极显著水平(P<0.01);各体尺指标两两均呈极显著正相关(P<0.01),其中,胸围与腹围的相关性最高,公、母牛相关系数分别为0.948、0.939;腹围与管围之间的相关性最低,相关系数分别为0.797和0.851。本试验结果可为明确安格斯牛选育方向,开展其遗传育种、品种资源开发利用提供基础数据。 相似文献
1000.
试验对已筛选的11株瘤胃源芽孢杆菌的产酶活力、耐酸、耐胆盐和抑菌活性及菌株间相容性进行研究。采用3,5-二硝基水杨酸法(DNS法)测定纤维素酶的活力,涂布平板计数法测定菌株的耐酸性、耐胆盐等特性,牛津杯法测定抑菌活性,滤纸片法进行室内相容性试验。结果显示,菌株T-2、T-3和T-10产纤维素酶的酶活力较高;菌株T-2、T-5、T-7、T-8和T-9表现较强的耐酸能力;菌株T-4、T-5、T-9和T-11表现较强的耐胆盐能力。菌株T-4和T-7对副溶血弧菌的抑菌活性较强,T-7对产气杆菌的抑菌活性最强,T-5、T-9、T-10对大肠杆菌抑菌活性较强,而T-3对藤黄八叠球菌指示菌的抑菌性最强。室内相容性试验结果表明,11株芽孢杆菌部分菌株之间存在颉颃作用,由此筛选出优良菌株的复配组合,抑菌可能的最优复配组合为T-3、T-4、T-5;T-3、T-4、T-10;T-4、T-5、T-10;T-3、T-7、T-10。纤维素酶活力较强时可能的最优复配组合为T-2、T-3、T-5;T-2、T-3、T-10;T-2、T-5、T-10;T-3、T-5、T-6;T-3、T-5、T-10。本试验可为动物饲料的研究和开发提供一定的参考依据。 相似文献