Effect of technological processing on the allergenicity of mangoes (Mangifera indica L.)

In parallel with the rising popularity of exotic fruits in Europe, allergy against mango is of increasing importance. Because mangoes are also consumed as processed products such as chutneys or beverages, the influences of different process conditions on their allergenicity were investigated. Mango purees and nectars were manufactured at small pilot-plant scale, and the allergenic potencies of the resulting intermediate and final products were determined by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting and inhibitive enzyme allergosorbent tests (EAST-inhibition), using a pool serum of 9 individuals with manifest mango allergy. The mango allergens were shown to be very stable during technological processing. Irrespective of enzymatic matrix decomposition, mechanical tissue disintegration and heating during peeling, mash treatment, and pasteurization, significant loss of allergenicity could not be observed in the extracts of mango purees and nectars derived thereof. These results were confirmed by analogous investigation of commercial mango drinks and nectars. Hence, conventional mango processing into pulp-containing products typical for this species obviously does not allow complete elimination of the allergenic potency.     

Impact of Pollutant Elements on Vegetables Growing in Sewage-Sludge-Treated Soils

Samples from potato (Solanum tubersosum), cauliflower (Bropica Oleracu var. Botryhs), and cabbage (Brassica Olerace var. Capitela) and soil samples were collected from fields receiving sewage sludge loads for a long periods and were analyzed for their total concentration of chromium (Cr), cadmium (Cd), lead (Pb), nickel (Ni), and cobalt (Co) in different plant parts and in soil samples. Estimation of these pollutant elements was also made in the extract of the rooting zone (soil) of these plants. The accumulation of pollutant elements was higher in roots than in leaves and shoots, whereas the soil nearer to the discharge point had the maximum DTPA-CaCl₂ extractable pollutant elements and their concentrations diminished with distance. In soil samples, accumulation of the pollutant elements occurred in the order Pb > Cr > Ni > Cd > Co. In vegetables, the rate of accumulation was in the order potato > cauliflower > cabbage. The growth of the vegetable plants on contaminated soil was depressed, young leaves showed marked chlorosis, and brown necrotic spots later developed on almost the entire foliage of plants. With increase in age, the affected leaves had a wilted look. The vegetables growing near the discharge point contained the maximum amounts of heavy metals, which were above toxic limits. Of these elements, the accumulation of Cr by potato was highest.     

Involvement of Excess Cadmium on Oxidative Stress and Other Physiological Parameters of Eggplant

To examine tolerance of cadmium (Cd) by eggplant (Solanum melongena L.) cv. ‘Hybrid PK 123’, plants were grown in refined sand in complete nutrient solution for 52 days in a glasshouse at ambient temperature. Cadmium sulfate was superimposed on day 53, at variable levels: nil, 0.05, 0.1, 0.2, 0.4, and 0.5 mM. Influence of excess Cd was discernible after 5 days of metal supply at 0.4 and 0.5 mM Cd as depression in growth. At these levels, foliar symptoms were initiated as paling of young leaves at the base progressing upward. With increase in age, affected leaves turned golden yellow and these effects spread to lower leaves. Leaf size and floral initiation were very much restricted. These types of phenotypes induced leaf senescence. Excess Cd reduced the biomass and fruit yield of plants. At higher levels (>0.1 mM) of Cd, fruit formation was completely inhibited and fruits formed at 0.05 and 0.1 mM Cd were smaller in size. Besides this, excess Cd disturbed the metabolism of eggplant by reducing the concentration of chlorophyll (a and b), protein, Hill reaction activity, and activity of antioxidant enzymes—catalase and ascorbate peroxidase; whereas the activity of peroxidase and ribonuclease increased in leaves of eggplant. Cadmium excess reduced the concentration of Fe and Zn and Cd increased that of proline, lipid peroxidation, phenols, reducing sugars and Cd concentration in leaves of eggplant.     

The influence of land-use change on the organic carbon distribution and microbial respiration in a volcanic soil of the Chilean Patagonia

Land-use changes can modify soil carbon contents. Depending on the rate of soil organic matter (SOM) formation and decomposition, soil-vegetation systems can be a source or sink of CO₂. The objective of this study was to determine the influence of land-use change on SOM distribution, and microbial biomass and respiration in an Andisol of the Chilean Patagonia. Treatments consisted of degraded natural prairie (DNP), thinned and pruned Pinus ponderosa plantations (PPP), and unmanaged second-growth Nothofagus pumilio forest (NPF). The soil was classified as medial, amorphic, mesic Typic Hapludands. Soil microbial respiration and microbial biomass were determined in the laboratory from soil samples taken at 0–5, 5–10, 10–20 and 20–40 cm depths obtained from three pits excavated in each treatment. Physical fractionation of SOM was performed in soil of the upper 40 cm of each treatment to obtain the three following aggregate-size classes: macroaggregates (>212 μm), mesoaggregates (212–53 μm) and microaggregates (<53 μm). Plant C content was 68% higher in PPP than in DNP and 635% higher in NPF than in PPP. Total soil and vegetation C content in both DNP and PPP were less than half of that in NPF. Total SOC at 0–10 cm depth decreased in the order DNP (7.82%) > NPF (6.16%) > PPP (4.41%), showing that land-use practices affected significantly (P < 0.01) SOC stocks. In all treatments, microbial biomass C and respiration were significantly higher (P < 0.05) in the upper 5 cm. Soil microbial respiration was also correlated positively with microbial biomass C and SOC. The different land uses affect the formation of organic matter, SOC and microbial biomass C, which in turn will affect soil microbial respiration. Conversion of DNP to PPP resulted in a 44% decrease of SOC stocks in 0–10 cm mineral soil. The largest amount of SOC was stabilized within the mesoaggregate fraction of the less disturbed system, NPF, followed by PPP. In the long term, formation of stable mesoaggregates in soils protected from erosion can behave as C sinks.     

Selected carbon fluxes in Pinus ponderosa-based silvopastoral systems, exotic plantations and natural pastures on volcanic soils in the Chilean Patagonia

This study was undertaken to measure certain carbon (C) fluxes in a Pinus ponderosa-based silvopastoral systems (SPS) in Patagonia, Chile. Results are compared to those from adjacent 18-year-old managed pine plantations (PPP) and natural prairie (PST). Litter decomposition was determined using the litterbag method. Leached soil solution was collected with tension lysimeters at 80 cm depth, and soil respiration evaluated in situ with the soda lime technique. Wind direction influenced the litterfall distribution in SPS, with 94 % falling in the tree strips and within 3 m on the east side. In the first 6 months the initial needle decomposition was low and less than 5 % of the initial mass. The overall soil organic carbon (SOC) and N contents (0–40 cm depth) decreased significantly in the order SPS > PST > PPP, and within SPS were greater in the alleys, starting at 2.5 m from the tree strip, with the highest values always eastward of the strip, suggesting influence by the wind direction. Total soil respiration decreased in the order PST > SPS > PPP and leached C decreased in the order PPP > PST > SPS. In general, the mean annual soil moisture in the pasture alleys of SPS was twice that of PST. The air/soil temperatures decreased significantly in the second year only in PST and at 2 m and beyond from the tree strip in SPS. Soil moisture varied significantly among treatments, but showed the strong influence exerted by trees in the creation of a favorable microclimate within the pasture alleys of SPS.     

Investigating maternal effects on production traits in Duroc pigs using animal and sire models

Variance components for production traits were estimated using different models to evaluate maternal effects. Data analysed were records from the South African pig performance testing scheme on 22 224 pigs from 18 herds, tested between 1990 and 2008. The traits analysed were backfat thickness (BFAT), test period weight gain (TPG), lifetime weight gain (LTG), test period feed conversion ratio (FCR) and age at slaughter (AGES). Data analyses were performed by REML procedures in ASREML, where random effects were successively fitted into animal and sire models to produce different models. The first animal model had one random effect, the direct genetic effects, while the additional random effects were maternal genetic and maternal permanent environmental effects. In the sire model, the random effects fitted were sire and maternal grand sire effects. The best model considered the covariance between direct and maternal genetic effects or between sire and maternal grand sire effects. Fitting maternal genetic effects into the animal model reduced total additive variance, while the total additive variance increased when maternal grand sire effects were fitted into the sire model. The correlations between direct and maternal genetic effects were all negative, indicating antagonism between these effects, hence the need to consider both effects in selection programmes. Direct genetic correlations were higher than other correlations, except for maternal genetic correlations of FCR with TPG, LTG and AGES. There has been direct genetic improvement and almost constant maternal ability in production traits as shown by trends for estimated (EBVs) and maternal breeding values (MBVs), while phenotypic trends were similar to those for EBVs. These results suggest that maternal genetic effects should be included in selection programmes for these production traits. Therefore, the animal–maternal model may be the most appropriate model to use when estimating genetic parameters for production traits in this population.     

Potential existence of multiple Tylenchulus semipenetrans biotypes in South Africa

The existence of multiple Tylenchulus semipenetrans biotypes within a citrus-producing region complicates the selection of nematode-resistant rootstocks for managing the slow decline of citrus. The potential existence of multiple T. semipenetrans biotypes in South Africa was investigated under greenhouse conditions on differential host plants using nematode isolates from 18 citrus-producing districts with at least six diverse soil groups and three different climates. After three nematode generations, the regional effects had no influence on final nematode population densities, whereas the differential host plants significantly segregated the nematode reproductive potentials. On Olea europaea reproductive potentials (eggs and juveniles/g) were zero, whereas those on Citrus jambhiri and Poncirus trifoliata ranged from 79 to 482 and 86 to 433, respectively. All T. semipenetrans isolates, regardless of the soil group and climate type, behaved similarly to the Poncirus biotype, which therefore rules out the existence of multiple T. semipenetrans biotypes in South Africa.     

An <Emphasis Type="Italic">in-vivo</Emphasis> study of the efficacy and safety of ethno-veterinary remedies used to control cattle ticks by rural farmers in the Eastern Cape Province of South Africa

Ticks feed on blood, are vectors of tick-borne diseases and cause considerable skin damage to livestock. They are commonly controlled using commercial acaricides, which are expensive to the rural farmers, causing them to resort to alternative tick control methods. The objective of this study was to assess the acaricidal properties and safety of some materials (Ptaeroxylon obliquum, Aloe ferox, Lantana camara, Tagetes minuta, Used engine oil and Jeyes fluid, used by rural farmers to control cattle ticks in the Eastern Cape Province, South Africa. A total of 52 cattle were divided into 13 experimental groups with 4 cattle in each. Jeyes fluid at 76.8% concentration and Used engine oil had an efficacy that was almost similar to that of the positive control Ektoban® (Cymiazol 17.5 and cypermethrin 2.5%). Extracts of L. camara at 40% concentration had an efficacy of 57% while A. ferox, P. obliquum and T. minuta were not effective. The test materials had no irritation effect on rats. The study revealed that the materials rural farmers use as acaricides vary in their efficacy in controlling ticks.     

Forage seed production and trade as a pathway out of poverty in the smallholder sector: lessons from the Zimbabwe Crop Livestock Integration for Food Security (ZimCLIFS) project

The major challenge to adoption of improved forage technologies in the smallholder farming sector is poor accessibility to seed of improved cultivars. Since 2012, the Zimbabwe Crop Livestock Integration for Improved Food Security (ZimCLIFS) project set out to address such challenges through research-for-development initiatives. The main objective was to demonstrate the potential viability of a pasture seed business using a lead farmer approach, farmer-to-farmer technology dissemination, innovation platforms and field demonstrations. Snapshot surveys were used to map forage seed distribution pathways within and outside project areas. Total land area planted to forages from the 2012/13 to 2014/15 seasons increased by 147% from 14.6 ha. In 2013, Mucuna pruriens (mucuna), Lablab purpureus (lablab) and Vigna unguiculata (cowpea) seed produced was 2 250 kg, 120 kg and 4 450 kg, respectively, and by the third season, total yield increased by 163%. Seed diffusion was within and beyond project boundaries, with mucuna (67%) and lablab (43%) seed produced in the 2013/14 season being distributed outside the project area. Highest amounts received by some farmers were US$800 and US$750 for lablab and mucuna, respectively. It was concluded that there is scope to develop formal pasture seed businesses to increase rural industrialisation and provide a pathway out of poverty in the smallholder sector.     

The effect of conditioning and natural aeration methods on rate of moisture loss and crude protein content of Lablab purpureus herbage during hay-making

The objective of this on-farm study was to develop an effective and labour-extensive method to cure forage Lablab purpureus ‘Highworth’ for resource-constrained smallholder farmers in the Goromonzi district, Zimbabwe in 2014. In a completely randomised factorial design, the drying rate of forage subjected to three conditioning treatments, viz. pressing with a plain water-filled 200 L steel drum, pressing with a water-filled 200 L steel drum covered with 14-gauge wire mesh, and chopping to 5?cm length with a machete, was compared with that of unconditioned forage. The conditioning treatments were subjected to three natural aeration methods, viz. Aframe, raised platform, and dry ground. Replicated four times, conditioned forage was cured at a swath density of 25?kg m?2 to cover 3?m2?per treatment over 28 d. Conditioning of forage had a significant (p < 0.05) effect on drying rate. However, conditioning did not have a significant (p > 0.05) effect on crude protein content. Aeration treatments did not have a significant effect (p > 0.05) on drying rate or crude protein content. Moisture loss rate was higher for forage conditioned with a wired drum and machete. Conditioning with a machete was most labour intensive compared with drum conditioning treatments. Laceration and pressing is an effective and labour-extensive conditioning method for resource-constrained smallholder farmers.