全文获取类型
收费全文 | 398篇 |
免费 | 29篇 |
专业分类
林业 | 22篇 |
农学 | 31篇 |
41篇 | |
综合类 | 33篇 |
农作物 | 55篇 |
水产渔业 | 57篇 |
畜牧兽医 | 160篇 |
园艺 | 3篇 |
植物保护 | 25篇 |
出版年
2023年 | 2篇 |
2022年 | 8篇 |
2021年 | 21篇 |
2020年 | 10篇 |
2019年 | 14篇 |
2018年 | 15篇 |
2017年 | 3篇 |
2016年 | 8篇 |
2015年 | 13篇 |
2014年 | 16篇 |
2013年 | 20篇 |
2012年 | 33篇 |
2011年 | 35篇 |
2010年 | 13篇 |
2009年 | 28篇 |
2008年 | 37篇 |
2007年 | 15篇 |
2006年 | 21篇 |
2005年 | 18篇 |
2004年 | 29篇 |
2003年 | 14篇 |
2002年 | 8篇 |
2001年 | 5篇 |
2000年 | 8篇 |
1999年 | 1篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 5篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1970年 | 2篇 |
1965年 | 1篇 |
1964年 | 4篇 |
1963年 | 3篇 |
排序方式: 共有427条查询结果,搜索用时 10 毫秒
61.
Jae-Won Byun Soon-Seek Yoon Gye-Hyeong Woo Byeong Yeal Jung Yi-Seok Joo 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(3):269-271
An outbreak of fatal hemorrhagic pneumonia with 70~90% morbidity and 50% mortality occurred in an animal shelter in Yangju, Gyeonggi Province, Korea. Clinically, the affected dogs showed severe respiratory distress within 48 h after arriving in the shelter. The dead were found mainly with nasal bleeding and hematemesis. At necropsy, hemothorax and hemorrhagic pneumonia along with severe pulmonary consolidation was observed, though histopathological analysis showed mainly hemorrhagic bronchopneumonia. Lymphoid depletion was inconsistently seen in the spleen, tonsil and bronchial lymph node. Gram-positive colonies were shown in blood vessels or parenchyma of cerebrum, lung, liver, spleen, and kidney. Also, Streptococcus (S.) equi subsp. zooepidemicus was isolated from the various organs in which the bacterium was microscopically and histologically detected. In addition, approximately 0.9 Kb specific amplicon, antiphagocytic factor H binding protein, was amplified in the bacterial isolates. In this study, we reported an outbreak of canine hemorrhagic bronchopneumonia caused by S. equi subsp. zooepidemicus in an animal shelter in Yangju, Korea. 相似文献
62.
Dae Sub Song Youn Jeong Lee Ok Mi Jeong Yong Joo Kim Chan Hee Park Jung Eun Yoo Woo Jin Jeon Jun Hun Kwon Gun Woo Ha Bo Kyu Kang Chul Seung Lee Hye Kwon Kim Byeong Yeal Jung Jae Hong Kim Jin Sik Oh 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(4):323-329
Active serologic surveillance is necessary to control the spread of the avian influenza virus (AIV). In this study, we evaluated a commercially-available cELISA in terms of its ability to detect AIV antibodies in the sera of 3,358 animals from twelve species. cELISA detected antibodies against reference H1- through H15-subtype AIV strains without cross reactivity. Furthermore, the cELISA was able to detect antibodies produced following a challenge of the AIV H9N2 subtype in chickens, or following vaccination of the AIV H9 or H5 subtypes in chickens, ducks and geese. Next, we tested the sensitivity and specificity of the cELISA with sera from twelve different animal species, and compared these results with those obtained by the hemagglutination-inhibition (HI) test, the "gold standard" in AIV sera surveillance, a second commercially-available cELISA (IZS ELISA), or the agar gel precipitation (AGP) test. Compared with the HI test, the sensitivities and specificities of cELISA were 95% and 96% in chicken, 86% and 88% in duck, 97% and 100% in turkey, 100% and 87% in goose, and 91% and 97% in swine, respectively. The sensitivities and specificities of the cELISA in this study were higher than those of IZS ELISA for the duck, turkey, goose, and grey partridge sera samples. The results of AGP test against duck and turkey sera also showed significant correlation with the results of cELISA (R-value >0.9). In terms of flock sensitivity, the cELISA correlated better with the HI test than with commercially-available indirect ELISAs, with 100% flock sensitivity. 相似文献
63.
Yong Hoon Lee Seungdon Lee Dong Hee Lee Sang Hye Ji Hyun Young Chang Sunggi Heu Jae Wook Hyun Dong-Soo Ra Eun Woo Park 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(1):97-102
Soil-borne cereal mosaic virus (SBCMV) causes a severe disease in susceptible cultivars of winter wheat. The virus is vectored by the soil-borne protist
Polymyxa graminis. Experiments were conducted to investigate whether SBCMV RNA2 could persist in seed from SBCMV-infected susceptible cultivars
of winter wheat. Over 7,000 seedlings were generated from seed collected from two cultivars of SBCMV-infected winter wheat.
Seedlings were grown in a glasshouse compartment and batch tested for the presence of SBCMV using real-time RT-PCR. The majority
of batches tested positive for SBCMV, indicating an RNA2 transmission rate of 1.8–9.4% in wheat. The presence of the virus
was confirmed by amplifying and sequencing a larger (400 bp) fragment of viral RNA2 in a sub-set of the seedlings testing
positive by real-time RT-PCR. Root extracts from this sub-set tested negative for P. graminis using real-time PCR. The implications for disease epidemiology of this virus are discussed.
The authors are British Civil Servants and as such their work is subject to British Crown Copyright. This means the exclusive
copyright for the article cannot be transferred. 相似文献
64.
65.
In Brassicaceae crop breeding programs, wild relatives have been evaluated as genetic resources to develop new cultivars with biotic and abiotic stress resistance. This has become necessary because of the diversification of ecotypes of diseases and pests, changing food preferences, advances in production technology, the use of new approaches such as in vitro breeding programs, and the need for economical production of F1 seed. To produce potential new cultivars, interspecific and intergeneric hybridizations have been performed between cultivated species and between cultivated species and their wild relatives. Furthermore, interspecific and intergeneric hybrids have been successfully produced using embryo rescue techniques. In this paper, we review the interspecific and intergeneric incompatibilities between Brassicaceae crops and their wild relatives, and the production, characterization, and improvement of synthetic amphidiploid lines, alien gene introgression lines, alloplasmic lines, monosomic alien chromosome addition lines, and monosomic alien chromosome substitution lines. The goal is to provide useful materials to support practical breeding strategies and to study the genetic effects of individual chromosomes on plant traits, the number of genes that control a trait, their linkage relationships, and genetic improvement in Brassicaceae crops. 相似文献
66.
Hee Ock Boo Jeong Hun Park Hag Hyun Kim Soo Jeong Kwon Sun Hee Woo 《Journal of Crop Science and Biotechnology》2018,21(5):507-514
This study was conducted to investigate cytotoxic effect, phenolic content, DPPH, ABTS radical scavenging rate and nitrite scavenging rate from different solvent fractions of Codonopsis lanceolate root. At all extracts concentration, the cytotoxic effect on different fractions against human cancer cells was higher in n-hexane and butyl alcohol fractions than in the other fractions. The IC50 value on HeLa cell showed the lowest by 62.70 μg.mL-1 on n-hexane fraction, and exhibited the values of butyl alcohol fraction 341.36 μg.mL-1, methylene chloride 598.33 μg.mL-1, ethyl acetate fraction 860.44 μg.mL-1, DW fraction 2896.82 μg.mL-1. Total polyphenol content on different solvent fractions varied from 102.43 to 153.52 mg.g-1, and that of ethyl acetate fraction showed the highest amount. The DPPH and ABTS radical scavenging activity showed that the increase was proportional to the concentration, and the scavenging activity also showed the highest in ethyl acetate fraction. The nitrite scavenging activity of each fraction at pH 1.2 was in the order of EA > BA > MC > n-H > DW, and the lower the acidity, the higher nitrite scavenging activity, and there was no distinct detection of nitrite scavenging effects of the pH range 6.0. The results of this study suggested that the extract of Codonopsis lanceolate root may assist in the potential biological activities, and it was found that the activity was different depending on the organic solvent fraction and the water fraction. 相似文献
67.
Direct observation and quantification of CO₂ binding within an amine-functionalized nanoporous solid
Vaidhyanathan R Iremonger SS Shimizu GK Boyd PG Alavi S Woo TK 《Science (New York, N.Y.)》2010,330(6004):650-653
Understanding the molecular details of CO(2)-sorbent interactions is critical for the design of better carbon-capture systems. Here we report crystallographic resolution of CO(2) molecules and their binding domains in a metal-organic framework functionalized with amine groups. Accompanying computational studies that modeled the gas sorption isotherms, high heat of adsorption, and CO(2) lattice positions showed high agreement on all three fronts. The modeling apportioned specific binding interactions for each CO(2) molecule, including substantial cooperative binding effects among the guest molecules. The validation of the capacity of such simulations to accurately model molecular-scale binding bodes well for the theory-aided development of amine-based CO(2) sorbents. The analysis shows that the combination of appropriate pore size, strongly interacting amine functional groups, and the cooperative binding of CO(2) guest molecules is responsible for the low-pressure binding and large uptake of CO(2) in this sorbent material. 相似文献
68.
Woong Yoon Jung Min Ryu Min Young Lee Yong Ju Moon Sang Hun Lee Jae Hong Park Seung Pil Yun Min Woo Jang Sung Su Park Ho Jae Han 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(3):185-189
Micropigs are the most likely source animals for xenotransplantation. However, an appropriate method for evaluating the lung of micropigs had not been established. Therefore, this study was performed to evaluate the feasibility of 64-channel multi-detector row computed tomography (MDCT) to measure the diameter of the pulmonary arteries and the lung volume in micropigs. The mean diameters of the trachea, and left and right bronchi were 1.6 ± 0.17, 1.18 ± 0.14, and 1.1 ± 0.11 cm, respectively. The mean diameters of the main, right, and left pulmonary arteries were 1.38 ± 0.09, 1.07 ± 0.26, and 0.98 ± 0.13 cm and the diameters of right, left, and common inferior pulmonary veins were 0.97 ± 0.20, 0.76 ± 0.20, and 1.99 ± 0.26 cm, respectively. The mean lung volume was 820.3 ± 77.11 mL. The data presented in this study suggest that the MDCT may be a noninvasive, rapid, and accurate investigational method for pulmonary anatomy in living lung donors. 相似文献
69.
Ko JA Kim YM Ryu YB Jeong HJ Park TS Park SJ Wee YJ Kim JS Kim D Lee WS 《Journal of agricultural and food chemistry》2012,60(24):6210-6216
Rubusoside (R) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method. A stevioside (ST)-specific β-glucosidase (SSGase) was selected and purified 7-fold from Aspergillus aculeatus Viscozyme L by a two-step column chromatography procedure. The 79 kDa protein was stable from pH 3.0 to pH 7.0 at 50-60 °C. Hydrolysis of ST by SSGase produced R and steviol monoglucosyl ester as determined by (1)H and (13)C nuclear magnetic resonance (NMR). Importantly, SSGase showed higher activity toward ST than other β-linked glucobioses. The optimal conditions for R production were 280 mM ST and 16.6 μL of SSGase at pH 5.1 and 63 °C. This is the first discussion detailing the production of R by enzymatic hydrolysis of ST and is useful for the food additive and pharmaceutical industries. 相似文献
70.
Park WT Kim JK Park S Lee SW Li X Kim YB Uddin MR Park NI Kim SJ Park SU 《Journal of agricultural and food chemistry》2012,60(33):8111-8116
We profiled and quantified glucosinolates, anthocyanins, carotenoids, and other secondary metabolites in the skin and flesh of pale green and purple kohlrabis. Analysis of these distinct kohlrabis revealed the presence of 8 glucosinolates, 12 anthocyanins, 2 carotenoids, and 7 phenylpropanoids. Glucosinolate contents varied among the different parts and types of kohlrabi. Glucoerucin contents were 4-fold higher in the flesh of purple kohlrabi than those in the skin. Among the 12 anthocyanins, cyanidin 3-(feruloyl)(sinapoyl) diglucoside-5-glucoside levels were the highest. Carotenoid levels were much higher in the skins than the flesh of both types of kohlrabi. The levels of most phenylpropanoids were higher in purple kohlrabi than in pale green ones. trans-Cinnamic acid content was 12.7-fold higher in the flesh of purple kohlrabi than that in the pale green ones. Thus, the amounts of glucosinolates, anthocyanins, carotenoids, and phenylpropanoids varied widely, and the variations in these compounds between the two types of kohlrabi were significant. 相似文献