蜂花粉抗化学性肝损伤研究进展

近年来研究发现蜂花粉具有显著的保肝功效,国内外学者已对此开展了广泛而深入的研究。本文就蜂花粉及活性成分对化学性肝损伤(四氯化碳肝损伤、酒精性肝损伤、氨基半乳糖诱导肝损伤)的作用效果和作用机理等方面的研究进展进行了综述,并对其研究方向和前景进行展望。     

Treatment of experimental ureteral strictures by endourological ureterotomy and implantation of stents in the porcine animal model

The objective of this study is to evaluate the dilation of the ureter using endoureterotomy and an expanding-sheath double pigtail ureteral stent in the treatment of experimentally induced ureteral strictures in the porcine animal model. This is a new treatment in the ureteral strictures resolution in Veterinary Urology, although it is not a common affection, it usually appears as a consequence of ureteritis and in the iatrogenic female genital surgery. The experimental study is design in three phases: induction of experimental stricture, diagnosis and treatment of the stricture and follow-up. We have used 10 healthy Large White female pigs. The internal ureteral diameter was measured prior to laparoscopic ligature stricture induction using retrograde ureteropyelography (RUPG). Experimental stricture was diagnosed 4 weeks after intervention, using RUPG and ultrasound, and treated by endoureterotomy and subsequent placement of a double pigtail ureteral stent, which was removed 6 weeks later. The study finished 4 weeks later with measurement of ureteral diameters using RPUG and ultrasound evaluation. Except in one case, all ureters displayed permanent dilation of the strictured area for 10 weeks after treatment (6 weeks with ureteral stent and 4 more weeks without stent). Finally, this technique proved to be effective in cases of short-length and short-living ureteral strictures, and represents a viable alternative to conventional surgery in animals.     

用重组核衣壳蛋白ELISA检测PRRSV抗体的研究

ＰＲＲＳＶ重组Ｎ蛋白ＥＬＩＳＡ检测人工感染猪血清７５份,与ＩＤＥＸＸ公司ＥＬＩＳＡ试剂盒及ＩＦＡ的符合率分别为９７．３％和１００％。检测疫苗免疫猪血清,阳性率１００％。在免疫后６ｄ就能检出抗体,比ＩＦＡ敏感。检测８５份我国田间猪血清,阳性率１８．８％。     

低成本省力化栽桑技术的研究进展

我国已育成了若干适合条桑育,适于扦插的桑品种及适于种子直播的杂交桑,为建立高密度低成本桑园打下了基础。推广了直接用袋接体,硬枝扦插和杂交种直接建园和初试了中晚秋条桑收获技术。今后的研究方向是选育发根性强,高产优势,适合条桑收获的桑树品种及以条桑收获为主的提高劳动生产率的栽培技术。     

温度、光照和发芽床对假俭草种子萌发的影响

实验室内进行了假俭草种子净度检验样品最低限量和发芽检验方法的研究，探讨了光照（以黑暗为对照）、温度（分别包括２０℃恒温，１５／２５℃、２０／３０℃、２０／３５℃和２５／３５℃变温）和发芽床（分设纸上ＴＰ和砂中Ｓ）等因素对种子发芽的影响。结果表明：假俭草种子净度检验样品最低限量是３ｇ，送验样最低量以及计数其它植物种子检验样品最低量均为３０ｇ；最佳萌发条件为２０／３０℃或２０／３５℃变温（１６小时低温，８小时高温），高温时段设光照，和纸上（ＴＰ）发芽；发芽的初次和未次计数时间分别以第１０天和第２１天为宜；种子的幼苗发育可划分为单子叶植物子叶留土类型。     

不同激素剂量对幼鼠超数排卵及超排卵质量的影响

应用国产激素制剂对３～８周龄昆明种小白鼠进行了超数排卵处理,重点观察了不同激素剂量对幼龄小鼠的超排效果及超排卵质量的影响。实验结果表明,以ＰＭＳＧ与ｈＣＧ各１０ＩＵ剂量超排３～８周龄小鼠,５周龄小鼠的超排效果（平均获卵８３．８±１０．２个）极显著（Ｐ＜０．０１）好于４～８周龄小鼠的超排效果（平均获卵３９．２～４９．８个）,而３周龄小鼠超排卵受精后体外培养的卵裂率（９７．１％）、桑椹胚率（８６．８％）和囊胚率（６５．１％）与４～８周龄超排卵的（分别为８５．５％～１００．０％、９４．９％～１００．０％和６３．２％～８７．７％）相近;以ＰＭＳＧ与ｈＣＧ各５ＩＵ剂量超排３～５周龄小鼠,３周龄小鼠的超排效果（平均获卵５９．０±６．５个）显著（Ｐ＜０．０５）好于４周龄小鼠的超排效果（平均获卵３１．４±４．９个）,并极显著（Ｐ＜０．０１）好于５周龄小鼠的超排效果（平均获卵２１．４±３．３个）;以ＰＭＳＧ与ｈＣＧ各１５ＩＵ剂量超排３～５周龄小鼠,３周龄小鼠的超排效果（平均获卵４８．６±７．３个）显著（Ｐ＜０．０５）好于５周龄小鼠的超排效果（平均获卵２５．２±５．８个）,也好于４周龄小鼠的超排效果（平均获卵４０．２±５．１个     

热敏电阻冰点仪法测定生鲜牛乳中冰点的研究

利用热敏电阻冰点仪法测定生鲜牛乳中的冰点,对该方法的重复性、灵敏度以及掺水掺盐对生鲜牛乳冰点值的影响等方面进行了研究.此方法的检测极限值为2.4 m℃.     

牛副结核检疫中的非特异性干扰

给犊牛接种各种分枝杆菌，然后以副结核ＰＰＤ进行变态反应试验，观察各分枝杆菌感染对副结核变态反应的干扰；从各种分枝杆菌感染牛群采取血清进行副结核ＥＬＩＳＡ检测，以观察各分枝杆菌血清对牛副结核ＥＬＩＳＡ的干扰。结果：多种分枝杆菌，特别是鸟胞内分枝杆菌、瘰疬分枝杆菌的感染可干扰副结核变态反应；部分结核牛血清干扰副结核ＥＬＩＳＡ的检测     

LFA—3和IL—2对机体免疫增强作用

以绵羊红细胞膜提取的ＬＦＡ－３和猪外周血单个核细胞所产生的ＩＬ－２，同时或分别与新城疫Ⅳ系疫苗胸肌注射２２日龄雏鸡，测定鸡体ＨＩ抗体效阶及Ｅｔｇ花环形成率的变化，结果表明，ＬＦＡ－３组抗体效价与对照组差异极显著，ＩＬ－２也可显著提高ＩＶ系苗免疫后ＨＩ抗体效价，且两者的作用具有剂量依赖性。