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91.
Young Hoon Park Seong Gyu Ahn Young Mi Choi Hye Jeong Oh Dong Chun Ahn Jin Gi Kim Jum Soon Kang Young Whan Choi Byoung Ryong Jeong 《Scientia Horticulturae》2010
The ‘Genome database for Rosaceae (GDR)’ provides a large collection of expressed sequence tags (ESTs) harboring simple sequence repeats (SSRs) from several Rosaceae genera, including Rosa (rose). Primer pairs flanking SSR were designed for 312 unique Rosa ESTs based on GDR database. Eight rose (Rosa hybrida L.) genotypes were tested for PCR amplification, and 287 (92%) of the primer pairs generated allele-specific PCR bands that were readily scored. From 183 (63.7%) primer pairs that evidenced polymorphic alleles among the eight rose cultivars, 20 pairs evidencing EST sequence homology to known gene functions and high levels of polymorphism were selected and utilized for DNA fingerprinting and genetic diversity assessments of 47 rose hybrids. A total of 202 polymorphic bands were scored and generated unique fingerprints for each rose hybrid. The Nei–Li genetic similarity coefficients among 1081 pair-wise comparisons of 47 cultivars exhibited a broad range of genetic variations from 0.30 (‘Grand King’ and ‘Carnival’) to 0.99 (‘First Red’ and ‘Red Champ’). UPGMA cluster analysis divided 47 hybrids into five major groups and two sub-groups. The cross-species transferability of 273 EST-SSR primer pairs was evaluated using four genotypes of the strawberry, a genus member of the Rosaceae family. PCRs on the DNA samples of strawberry were successful for 165 primer pairs; among these, 123 pairs amplified 243 polymorphic bands. As surrogates of the marker transfer, the phenetic relationship among the four strawberry genotypes was evaluated. Genetic similarity coefficients varied from 0.78 (‘Maehyang’ and ‘Janghyee’) to 0.64 (‘Janghyee’ and ‘Pragana’). The results of cluster analysis showed that the three octaploid strawberry cultivars were quite similar, whereas the diploid ‘Pragana’ was related distantly at the genomic DNA level. The EST-SSR markers developed in the present study can be efficiently utilized for genetic diversity studies in Rosaceae. 相似文献
92.
Jeong O-Young Park Hyeon-Su Baek Man-Kee Kim Woo-Jae Lee Geon-Mi Lee Chang-Min Bombay Maurene Ancheta Melanie Banzon Lee Jeom-Ho 《Journal of Crop Science and Biotechnology》2021,24(1):1-11
Journal of Crop Science and Biotechnology - Rice has been grown as a staple food in South Korea for several millennia. However, its status has not stayed constant as a result of changes in South... 相似文献
93.
Ko JA Kim YM Ryu YB Jeong HJ Park TS Park SJ Wee YJ Kim JS Kim D Lee WS 《Journal of agricultural and food chemistry》2012,60(24):6210-6216
Rubusoside (R) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method. A stevioside (ST)-specific β-glucosidase (SSGase) was selected and purified 7-fold from Aspergillus aculeatus Viscozyme L by a two-step column chromatography procedure. The 79 kDa protein was stable from pH 3.0 to pH 7.0 at 50-60 °C. Hydrolysis of ST by SSGase produced R and steviol monoglucosyl ester as determined by (1)H and (13)C nuclear magnetic resonance (NMR). Importantly, SSGase showed higher activity toward ST than other β-linked glucobioses. The optimal conditions for R production were 280 mM ST and 16.6 μL of SSGase at pH 5.1 and 63 °C. This is the first discussion detailing the production of R by enzymatic hydrolysis of ST and is useful for the food additive and pharmaceutical industries. 相似文献
94.
This research studies the factors which influence the tensile strength of tire cords. Five yarn samples are made by changing
the spinning conditions and viscosity to get various physical properties. Different twisting methods are introduced and the
yarns are twisted under different processing conditions for each twisting process. With the experimental results, various
analyses are performed to find the important factors in retaining strength after the twisting process. SEM and optical microscopic
photographs are taken along with some measurements to assist the analysis. 相似文献
95.
Sung Ok Sohn So Min Lee Yun Mi Kim Jeong Hyun Yeum Jin Hyun Choi Han Do Ghim 《Fibers and Polymers》2007,8(2):163-167
A durable aroma finishing for PET fabric was carried out by adopting poly(vinyl acetate) (PVAc) nanoparticles containing lavender
oil (LO) in core. Relatively small size of PVAc nanoparticles (ca. 244 nm of mean particle diameter) was expected to resist
the frictional destruction of the particles, which is frequently observed in cases of microcapsules. PVAc nanoparticles containing
LO in core were prepared by emulsification-diffusion method and their application as an aroma releasing agent for PET fabrics
was assessed through the observation of releasing profiles of LO in ethanol for experimental acceleration. Melamine-formaldehyde
(MF) microcapsules containing LO were also prepared and treated on fabrics for comparison. PVAc nanoparticles treated on PET
fabric showed higher initial releasing amount, which was ascribed to the enhanced surface area. After 2 days of releasing,
PET fabric treated with PVAc nanoparticles showed slower and more stable releasing profile and reached about 12 ppm of cumulative
release after 16 days, which was under two thirds of that with MF microcapsules. PVAc nanoparticles can be used as an agent
for durable aroma finishing of PET fabrics. 相似文献
96.
Youn?Hee?Jeon Won?Young?Jeong Jung?Woo?Park Seung?Kook?AnEmail author 《Fibers and Polymers》2003,4(4):151-155
The abrasion behavior of three kinds of warp knitted fabrics, which are normally used for upper sole of footwear, was evaluated.
We measured the changes of mechanical and structural properties of each sample as abrasion cycle increased. Each sample showed
similar trends in compression and surface properties but there were significant differences in abrasion rate among the samples.
The mechanical properties showed remarkable differences with directions. The frictional coefficient (MIU) of fabric surface
increased at the beginning of abrasion and decreased as abrasion cycles increased. The weight and thickness of the fabric
linearly decreased with abrasion cycles. The surface roughness (SMD) and the compressional resilience (RC) decreased as abrasion
cycles increased while compressional energy (WC) increased. 相似文献
97.
Ryu YB Jeong HJ Yoon SY Park JY Kim YM Park SJ Rho MC Kim SJ Lee WS 《Journal of agricultural and food chemistry》2011,59(12):6467-6473
Influenza A virus infections continue to pose a major threat to humans and several animal species. Neuraminidase (NA) is one of the most promising targets for the development of drugs against influenza viruses because of its critical role in the viral life cycle. During the course of a search for NA inhibitors from edible natural sources, we found that the ethyl acetate layer of ethanol extracts of Ecklonia cava showed extremely high NA-inhibitory activity (72.1% inhibition at 30 μg/mL). Bioactivity-guided fractionation of the ethyl acetate layer yielded five phlorotannins, identified as phloroglucinol (1), eckol (2), 7-phloroeckol (3), phlorofucofuroeckol (4), and dieckol (5). The inhibitory activities of these compounds (1-5) against NAs from group-1 (A/Bervig_Mission/1/18 [H1N1], A/PR/8/34 [H1N1]) and group-2 (A/Hong Kong/8/68 [H3N2], A/Chicken/Korea/MS96/96 [H9N2]) influenza A were evaluated to determine potencies and kinetic behavior. Analyses using various in vitro influenza A virus NA assays showed that all five phlorotannin derivatives were selective NA inhibitors. Of the phlorotannins, phlorofucofuroeckol (4) exhibited the most potent inhibitory activities toward group-1 NAs (IC?? values, 4.5 and 14.7 μM), whereas dieckol (5) potently inhibited group-2 NAs. Kinetic analyses indicated that compounds 1-5 were all noncompetitive. Notably, these noncompetitive inhibitors synergized with oseltamivir to enhance the NA-inhibitory effects of oseltamivir. 相似文献
98.
Journal of Plant Diseases and Protection - Plum pox virus (PPV) is one of the most destructive viral pathogens infecting stone fruit trees worldwide. As PPV causes a viral disease that requires... 相似文献
99.
Cold plasma of O2 or Ar was irradiated on hydrophobic Kapton surface to attenuate or remove the electrostatic potential. A measurement on charge
dissipation speed clarifies the obscure effect of plasma. These consequences reveal that O2 plasma etching is more effective than Ar plasma. After 30 days, the dissipation speed of accumulated charge on initially
etched sample has not changed under summar season. 相似文献
100.
Jeong Ho Song Tae Jo Kang Young Don Cho Sun Hyoung Lee Jeong Soo Kim 《Fibers and Polymers》2006,7(3):217-222
Propargyl alcohol was coupled to 2,4-hexadiyne-1,6-diol (HDD) and crystallized in the process of ultraviolet irradiation-induced
topochemical polymerization. The HDD polymer crystals were used as one component in the fabrication of organic photovoltaic
cells, in combination with fullerene as the electron acceptor. The various structures of the produced photovoltaic cells included
bilayer, trilayer, and bulk heterojunction structures. Their photovoltaic properties were analyzed in relation to crystal
structure, electrochemical properties, and band structure of the HDD polydiacetylene polymers. 相似文献