Improvement of Fertility in Artificially Inseminated Ewes Following Vaginal Treatment with Misoprostol Plus Terbutaline Sulphate

The effect of vaginal administration of misoprostol plus terbutaline sulphate 6 h prior to artificial insemination (AI) upon the site of AI (vaginal or cervical) and fertility was studied using a total of 87 estrous synchronized Serra da Estrela ewes (control n = 42 and treated n = 45). Artificial insemination was performed using refrigerated semen at 54–55 h after sponge removal. Lambing rate (fertility) and prolificacy were compared between control and treated ewes. The effect of the site of semen deposition on fertility was also evaluated. Prolificacy rate was not different between control (1.5) and treated (1.59) ewes. The proportion of cervical AI achieved in control (45.2%) and treated (37.8%) ewes was not significantly different. Overall, fertility was significantly lower in control than in treated ewes (42.9% vs 64.4%; p < 0.04). Fertility following vaginal AI was significantly lower for control for than treated ewes (30.4% vs 60.7%; p < 0.03) but the difference was smaller and not significant for cervical AI (control 57.9% vs 70.6%). It was concluded that vaginal administration of misoprostol plus terbutaline sulphate 6 h prior to artificial insemination did not affect the proportion of cervical inseminations but significantly improved the fertility of treated ewes. Although needing confirmation, it was hypothesized that drugs might have induced local secretory modifications leading to an increase of cervical ability to retain more viable spermatozoa for fertilization.     

美洲黑杨生长及基因表达差异

以9年生美洲黑杨50号、36号及F1子代为材料,对美洲黑杨亲子代的生长变异和杂种优势进行了研究,并利用mRNA差异显示技术对美洲黑杨亲子代的基因表达差异进行研究。结果表明:美洲黑杨F1子代间生长量存在丰富的遗传变异,树高平均变异系数是24.59%;胸径平均变异系数是29.88%。美洲黑杨F1子代在苗期有12.2%的单株生长超过双亲,其平均中亲优势和超亲优势分别为42.05%和32.51%;而9年生的中亲优势和超亲优势分别是22.7%和12.0%。美洲黑杨亲子代间的基因表达差异明显,共找到57个表达差异基因,表达存在着3种量的差异类型和6种质的差异类型。在所有基因表达中父本特异表达基因在杂种一代中受抑制型所占比例最高(21.1%),子代特异表达基因最少(5.3%),母本(50号)基因对子代杂种优势的贡献要大于父本。     

9-硝基喜树碱酯类衍生物的合成和结构表征

以10-羟基喜树碱为原料,经硝化反应制备中间体9-硝基-10-羟基喜树碱,再经亲核取代反应合成新的9-硝基喜树碱酯类衍生物。对反应路线的选择及反应中间体和终产物的结构进行了详细解析,化合物的结构经1HNMR、13C NMR、IR和MS(ESI)表征。结果表明:此合成路线反应条件温和,产物总转化率达到90.2%。     

反刍动物甲烷生成机制及调控

甲烷是反刍动物瘤胃正常消化的产物,但其排放不仅对空气环境造成污染,增加温室效应,并且造成饲料能量的损失。因此,减少反刍动物瘤胃内甲烷的生成量对提高饲料能量利用率和改善环境都具有重要的意义。文章综述了瘤胃中甲烷生成的机制及影响甲烷产生量的因素,详细介绍了控制瘤胃内甲烷产生量的措施。     

64-Channel multi-detector row CT angiographic evaluation of the micropigs for potential living donor lung transplantation

Micropigs are the most likely source animals for xenotransplantation. However, an appropriate method for evaluating the lung of micropigs had not been established. Therefore, this study was performed to evaluate the feasibility of 64-channel multi-detector row computed tomography (MDCT) to measure the diameter of the pulmonary arteries and the lung volume in micropigs. The mean diameters of the trachea, and left and right bronchi were 1.6 ± 0.17, 1.18 ± 0.14, and 1.1 ± 0.11 cm, respectively. The mean diameters of the main, right, and left pulmonary arteries were 1.38 ± 0.09, 1.07 ± 0.26, and 0.98 ± 0.13 cm and the diameters of right, left, and common inferior pulmonary veins were 0.97 ± 0.20, 0.76 ± 0.20, and 1.99 ± 0.26 cm, respectively. The mean lung volume was 820.3 ± 77.11 mL. The data presented in this study suggest that the MDCT may be a noninvasive, rapid, and accurate investigational method for pulmonary anatomy in living lung donors.     

Haemoglobin in normal and neoplastic canine mammary glands

Four types of globins for oxygen transport are known in vertebrates, and the haemoglobin is responsible for carrying oxygen in blood. In this study, we found that haemoglobin was also expressed in canine mammary glands. Samples were taken from 26 malignant mammary tumors, 16 normal mammary glands and 10 other normal tissues. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting and mass spectrometry were used to investigate haemoglobin in mammary tissues. The results indicated that normal canine mammary glands expressed high levels of haemoglobin protein as shown by Coomassie blue staining. The identity of haemoglobin was confirmed by immunoblotting and mass spectrometry, and the mass spectrometry data revealed that both alpha-haemoglobin and beta-haemoglobin were expressed. Relative to normal mammary glands, the levels of haemoglobin expression in mammary tumors were lower. Our results also indicated that the haemoglobin was endogenously produced in mammary gland tissues and was not derived from the erythroid cells.     

不同施肥方式对甜高粱产量和含糖量的影响

通过对甜高粱不同品种不同施肥方式产量和含糖量的影响研究,阐明不同甜高粱品种的产量性状和含糖量差异极显著;同一品种采用不同施肥方式产量的差异极显著,但含糖量差异不显著;不同品种与不同施肥方式互作对产量和含糖量的影响差异不显著。同时阐明,龙甜6和龙甜2产量高,含糖量高,在生产上采用的最佳施肥方式为7月5日一次性追施磷酸二铵150kg／hm2,尿素150kg／hm2,硫酸钾150kg／hm2。     

麦秸机械全量还田机插秧现状及关键技术

通过麦秸机械全量还田机插技术的应用,实现了秸秆还田和轻简稻作的有机结合,提高土壤有机质含量,改善土壤结构和环境质量,有利于水稻增产丰收,节本增效十分明显,推动了农业的可持续发展.     

东北林蛙卵蛋白抗疲劳活性研究

东北林蛙卵蛋白含量丰富,是林蛙产业中尚待开发利用的副产品。本试验研究了东北林蛙卵蛋白的抗疲劳活性。取雄性昆明小鼠,体重18~22g,随机分为空白对照组、林蛙卵蛋白(低、中、高)剂量3组,连续灌胃35d,分别测量小鼠的体重变化情况、负重游泳力竭时间、肝糖原和血乳酸的含量。结果表明,东北林蛙卵蛋白对小鼠体重增长无显著影响,可降低运动后小鼠血乳酸含量,增加小鼠负重游泳时间及肝糖原含量。研究显示,东北林蛙卵蛋白具有增加小鼠抗疲劳的活性作用。     

Antipruritic activity of extracts of Humulus scandens, the combinations of bioactive flavonoids

The antipruritic effects of the ethanol fractions of Humulus scandens on the 4-AP (4-aminopyridine)-induced and chloroquine-induced scratching in ICR mice were examined. The 40% ethanol fractions of H. scandens suppressed both the 4-AP- and chloroquine-induced scratching behavior, which significantly inhibited degranulation of rat peritoneal mast cell and antigen-stimulated histamine release. Further studies proved that the 40% ethanol fractions of H. scandens decreased the content of IL4 in serum of chloroquine-induced scratching ICR mice. The results suggest that the 40% ethanol fractions of H. scandens has antipruritic effects on both antihistamine-resistant and -sensitive pruritus.