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Fertility of 104 gilts artificially inseminated (AI) at a predetermined time (scheduled AI) after estrous synchronization with altrenogest (15 mg X gilt-1 X d-1 for 18 d) was compared with that of 103 gilts checked for estrus (estrus checked) and inseminated after altrenogest. Scheduled-AI gilts were inseminated once on d 5, 6 and 7 after the last altrenogest feeding (d 0). Estrus-checked gilts were exposed to a boar twice daily at 0830 and 1630 h and inseminated after the second and third estrous detection period following first detected estrus. Percentage of gilts assigned to treatment that farrowed (72.8 vs 67.3%), total pigs farrowed (11 +/- .4 vs 11.3 +/- .4) and pigs born alive (10.1 +/- .4 vs 10.5 +/- .4) were similar for estrus-checked and scheduled-AI gilts, respectively. We conclude that scheduled AI can be used with estrous synchronization for gilts and may have advantages in breeding herd management and the use of AI in swine. 相似文献
54.
Clinical exercise testing in the normal Thoroughbred racehorse 总被引:1,自引:0,他引:1
To evaluate normal cardiorespiratory and metabolic responses of Thoroughbred horses to a standardised treadmill exercise test, we examined 28 horses ranging in age from 1 to 4 years. The group consisted of eight yearlings, eight 2-year-olds and twelve 3 and 4-year-olds. All horses except the yearlings were in training, and either racing or ready to race, at the time of examination. None of the horses had histories of performance problems. On the first day the horses received a full physical examination, resting electrocardiogram, upper respiratory tract endoscopy and either one or two acclimatisation runs on the treadmill. The following day they were given an exercise test on a treadmill inclined at 6 degrees (+10% slope). The test consisted of 3 min at 4 m/sec, 90 sec at 6 m/sec and 60 sec intervals at 8, 10, 11, 12 and 13 m/sec. During the last 15 sec of each step, blood samples were collected for plasma lactate determination, expired respiratory gases were obtained using an open flow mask system for measurement of oxygen uptake, and heart rate was measured using telemetry electrocardiogram. From these measurements, various derived values were calculated, which have been used by others as indices of exercise capacity. These values included: V200 (speed at HR of 200 bpm), VHRmax (speed at which horses reached maximum HR), VO2-200 (oxygen uptake at a HR of 200 bpm), VO2max (maximum oxygen uptake), VLA4 (speed at which horses reached a plasma lactate of 4 mmol/l) and HRLA4 (HR at which horses reached a plasma lactate of 4 mmol/l). The yearlings had significantly lower values than the older age groups for most of the derived values.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
55.
Epstein-Barr virus (EBV), a ubiquitous human herpesvirus, has the ability to transform human B lymphocytes. No other cell type has been experimentally transformed by EBV, either by intact virions or naked viral DNA and subgenomic fragments. Two immortalized human T-lymphoblastoid cell lines have now been established by transfecting cord blood lymphocytes with purified B95-8 viral DNA enclosed in fusogenic Sendai virus envelopes (RSVE) and then exposing the cells to EBV from a P3HR-1 cell subclone. One of these lines, which has been fully characterized, is termed HBD-1. This line is positive for EBV DNA and expresses surface OKT11, OKT4, and Tac receptors, but not M-1, mu immunoglobulin chains, EBV receptors, or B-1 surface markers. The cells contain fully rearranged T-cell receptor genes and germline immunoglobulin genes. The karyotype of the cells is normal, they do not require interleukin-2 for growth, and do not contain human T-lymphotropic virus type I. However, the HBD-1 cells contain incomplete EBV genomes and express several EBV-determined antigens, including the early antigen type D, membrane antigens, but not EBV-determined nuclear antigen (EBNA). This association of the EBV genome with permanently growing hematopoietic cells of non B-cell lineage should prove useful in studies on the mechanism of EBV-mediated cell transformation. 相似文献
56.
To better understand the basis for human immunodeficiency virus type 1 (HIV-1) persistence and latency, the form in which viral DNA exists in the peripheral T lymphocyte reservoir of infected individuals was investigated. In asymptomatic individuals, HIV-1 was harbored predominantly as full-length, unintegrated complementary DNA. These extrachromosomal DNA forms retained the ability to integrate upon T cell activation in vitro. In patients with acquired immunodeficiency syndrome (AIDS), there was an increase in integrated relative to extrachromosomal DNA forms. By analysis of DNA from patient lymphocyte subpopulations depleted of human lymphocyte antigen-Dr receptor-positive cells, quiescent T cells were identified as the source of extrachromosomal HIV-1 DNA. Thus quiescent T lymphocytes may be a major and inducible HIV-1 reservoir in infected individuals. 相似文献
57.
PM Lundquist R Wortmann C Geletneky RJ Twieg M Jurich VY Lee CR Moylan DM Burland 《Science (New York, N.Y.)》1996,274(5290):1182-1185
The performance of amorphous organic photorefractive (PR) materials in applications such as optical data storage is generally limited by the concentration of active molecules (chromophores) that can be incorporated into the host without forming a crystalline material with poor optical quality. In polymeric PR systems described previously, performance has been limited by the necessity of devoting a large fraction of the material to inert polymer and plasticizing components in order to ensure compositional stability. A new class of organic PR materials composed of multifunctional glass-forming organic chromophores is described that have long-term stability and greatly improved PR properties. 相似文献
58.
The course of the Nile in northern Sudan follows a contorted path through Precambrian bedrock. Radar imagery shows that basement structures control the river's course in this region. Northward-flowing segments follow Precambrian fabrics, whereas east-west segments follow faults of much younger age. These faults may reflect recent uplift of the Nubian Swell and deflection of the river to the southwest to form the great bend of the Nile. 相似文献
59.
Neodymium-142 data for rocks from the Nuvvuagittuq greenstone belt in northern Quebec, Canada, show that some rock types have lower 142Nd/144Nd ratios than the terrestrial standard (epsilon142Nd = -0.07 to -0.15). Within a mafic amphibolite unit, 142Nd/144Nd ratios correlate positively with Sm/Nd ratios and produce a 146Sm-142Nd isochron with an age of 4280(-81)(+53) million years. These rocks thus sample incompatible-element-enriched material formed shortly after Earth formation and may represent the oldest preserved crustal section on Earth. 相似文献
60.