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21.
The endemic, anadromous cyprinidChalcalburnus tarichi is the only fish species known to occur in alkaline Lake Van (Eastern Anatolia, Turkey). EightC. tarichi were maintained individually in Lake Van water (17 – 19°C; pH 9.8; 153 mEq·I–1 total alkalinity; 22 total salinity) and tank water samples analyzed for 24 h in 2 to 4 h intervals. At zero time, < 1µM ammonia was present and urea was undetectable in the tank water; at 24 h, total ammonia and urea made up 114±32 and 35±25µM, respectively. Over the experimental period, ammonia-N and urea-N excretion averaged 1041±494 and 607±169moles·kg–1 fish·h–1, respectively. The extent of urea excretion was highly variable between specimens. Uric acid excretion was not detectable.Urea was present at high concentrations in all tissues and plasma (25 – 35moles·g–1·ml–1) of freshly caughtC. tarichi; total ammonia content of the tissues was by a factor of 1.9 (liver) to 3.0 (brain) lower. High arginase activity (2.4±0.2 U·min–1·g–1) was detected in the liver ofC. tarichi but ornithine carbamoylphosphate transferase, a key enzyme of the ornithine-urea-cycle, was absent. Ureagenesis is likely through degradation of arginine and/or uricolysis. High glutamine synthetase activity (11±0.6 U·min–1·g–1) and low ammonia content in brain suggest that, like other teleosts,C. tarichi has an efficient ammonia detoxification in the brain, but in no other tissue.Nitrogenous waste excretion at alkaline pH is discussed. The ability ofC. tarichi to excrete high levels of ammonia at extremely alkaline pH is unique among teleosts studied so far. The mechanism of ammonia excretion under Lake Van conditions remains to be elucidated. 相似文献
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Sudden pulse-like events of rapidly increasing CO2-efflux occur in soils under seasonally dry climates in response to rewetting after drought. These occurrences, termed “Birch effect”, can have a marked influence on the ecosystem carbon balance. Current hypotheses indicate that the “Birch” pulse is caused by rapidly increased respiration and mineralization rates in response to changing moisture conditions but the underlying mechanisms are still unclear. Here, we present data from an experimental field study using straight-forward stable isotope methodology to gather new insights into the processes induced by rewetting of dried soils and evaluate current hypotheses for the “Birch“-CO2-pulse. Two irrigation experiments were conducted on bare soil, root-free soil and intact vegetation during May and August 2005 in a semi-arid Mediterranean holm oak forest in southern Portugal. We continuously monitored CO2-fluxes along with their isotopic compositions before, during and after the irrigation. δ13C signatures of the first CO2-efflux burst, occurring immediately after rewetting, fit the hypothesis that the “Birch” pulse is caused by the rapid mineralization of either dead microbial biomass or osmoregulatory substances released by soil microorganisms in response to hypo-osmotic stress in order to avoid cell lyses. The response of soil CO2-efflux to rewetting was smaller under mild (May) than under severe drought (August) and isotopic compositions indicated a larger contribution of anaplerotic carbon uptake with increasing soil desiccation. Both length and severity of drought periods probably play a key role for the microbial response to the rewetting of soils and thus for ecosystem carbon sequestration. 相似文献
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While the possible negative effect of employer‐sponsored health insurance on labor mobility (job lock) has spawned substantial research, little research has considered differences in job lock between rural and urban areas. Job lock might differ in rural areas because of specific features of rural economies, in particular, the thinness of rural labor markets. This paper estimates measures of job lock for rural and urban areas. Parameter estimates suggest that it is larger in rural areas. The results also indicate that nonwage benefits such as retirement plans, paid vacation, and sick pay are more important factors in married rural workers decisions to change jobs while wages are a more important factor in the transition decisions of single urban workers. 相似文献
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Valle L Serena-Acedo T Liyanarachchi S Hampel H Comeras I Li Z Zeng Q Zhang HT Pennison MJ Sadim M Pasche B Tanner SM de la Chapelle A 《Science (New York, N.Y.)》2008,321(5894):1361-1365
Much of the genetic predisposition to colorectal cancer (CRC) in humans is unexplained. Studying a Caucasian-dominated population in the United States, we showed that germline allele-specific expression (ASE) of the gene encoding transforming growth factor-beta (TGF-beta) type I receptor, TGFBR1, is a quantitative trait that occurs in 10 to 20% of CRC patients and 1 to 3% of controls. ASE results in reduced expression of the gene, is dominantly inherited, segregates in families, and occurs in sporadic CRC cases. Although subtle, the reduction in constitutive TGFBR1 expression alters SMAD-mediated TGF-beta signaling. Two major TGFBR1 haplotypes are predominant among ASE cases, which suggests ancestral mutations, but causative germline changes have not been identified. Conservative estimates suggest that ASE confers a substantially increased risk of CRC (odds ratio, 8.7; 95% confidence interval, 2.6 to 29.1), but these estimates require confirmation and will probably show ethnic differences. 相似文献
28.
CX3CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance 总被引:3,自引:0,他引:3
Niess JH Brand S Gu X Landsman L Jung S McCormick BA Vyas JM Boes M Ploegh HL Fox JG Littman DR Reinecker HC 《Science (New York, N.Y.)》2005,307(5707):254-258
Dendritic cells (DCs) and macrophages are critical to innate and adaptive immunity to the intestinal bacterial microbiota. Here, we identify a myeloid-derived mucosal DC in mice, which populates the entire lamina propria of the small intestine. Lamina propria DCs were found to depend on the chemokine receptor CX3CR1 to form transepithelial dendrites, which enable the cells to directly sample luminal antigens. CX3CR1 was also found to control the clearance of entero-invasive pathogens by DCs. Thus, CX3CR1-dependent processes, which control host interactions of specialized DCs with commensal and pathogenic bacteria, may regulate immunological tolerance and inflammation. 相似文献
29.
Manzke T Guenther U Ponimaskin EG Haller M Dutschmann M Schwarzacher S Richter DW 《Science (New York, N.Y.)》2003,301(5630):226-229
Opiates are widely used analgesics in anesthesiology, but they have serious adverse effects such as depression of breathing. This is caused by direct inhibition of rhythm-generating respiratory neurons in the Pre-Boetzinger complex (PBC) of the brainstem. We report that serotonin 4(a) [5-HT4(a)] receptors are strongly expressed in respiratory PBC neurons and that their selective activation protects spontaneous respiratory activity. Treatment of rats with a 5-HT4 receptor-specific agonist overcame fentanyl-induced respiratory depression and reestablished stable respiratory rhythm without loss of fentanyl's analgesic effect. These findings imply the prospect of a fine-tuned recovery from opioid-induced respiratory depression, through adjustment of intracellular adenosine 3',5'-monophosphate levels through the convergent signaling pathways in neurons. 相似文献
30.
Rendulic S Jagtap P Rosinus A Eppinger M Baar C Lanz C Keller H Lambert C Evans KJ Goesmann A Meyer F Sockett RE Schuster SC 《Science (New York, N.Y.)》2004,303(5658):689-692
Predatory bacteria remain molecularly enigmatic, despite their presence in many microbial communities. Here we report the complete genome of Bdellovibrio bacteriovorus HD100, a predatory Gram-negative bacterium that invades and consumes other Gram-negative bacteria. Its surprisingly large genome shows no evidence of recent gene transfer from its prey. A plethora of paralogous gene families coding for enzymes, such as hydrolases and transporters, are used throughout the life cycle of B. bacteriovorus for prey entry, prey killing, and the uptake of complex molecules. 相似文献