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71.
Dispersion is an important issue for clay leaching in soils. In paddy soils of the Red River Delta (RRD), flooding with fresh water and relatively high leaching rates can accelerate dispersion and the translocation of clay. For the clay fraction of the puddled horizon of a typical paddy soil of the RRD, the effect of various cations and anions as well as humic acid (HA) at different pH values on the surface charge (SC) were quantified and the dispersion properties were determined in test tubes and described by the C50 value. In the <2 µm fraction, dominated by illite, the proportion of 2:1 vs. 1:1 clay minerals is 5:1. The organic‐C content of the clay fraction is 2.2%. Surface charge was found to be highly pH‐dependent. At pH 8 values of –32 and at pH 1 of –8 mmolc kg–1 were obtained. Complete dispersion was observed at pH > 4, where SC is > –18 mmolc kg–1. The flocculation efficiency of Ca strongly depends on the pH. At pH 4, the C50 value is 0.33, 0.66 at pH 5, and 0.90 mmol L–1 at pH 6. At pH 6, close to realistic conditions of paddy soils, the effect of divalent cations on the SC and flocculation decreases in the order: Pb > Cu > Cd > FeII > Zn > Ca > MnII > Mg; FeII was found to have a slightly stronger effect on flocculation than Ca. An increase in concentrations of Ca, MnII, and Mg from 0 to 1 mmol L–1 resulted in a change in SC from –25 to approx. –15 mmolc kg–1. In comparison, the divalent heavy‐metal cations Pb, Cu, Cd, and Zn were found to neutralize the SC more effectively. At a Pb concentration of 1 mmol L–1, the SC is –2 mmolc kg–1. From pH 3 to 5, the dispersion of the clay fraction is facilitated rather by SO than by Cl–, which can be explained by the higher affinity of SO to the positively charged sites. With an increase of the amount of HA added, the SC continuously shifts to more negative values, and higher concentrations of cations are needed for flocculation. At pH 3, where flocculation is usually observed, the presence of HA at a concentration of 40 mg L–1 resulted in a dispersion of the clay fraction. While high anion concentrations and the presence of HA counteract flocculation by making the SC more negative, FeII and Ca (C50 at pH 6 = 0.8 and 0.9 mmol L–1, respectively) are the main factors for the flocculation of the clay fraction. For FeII and Ca, the most common cations in soil solution, the C50 values were found to be relatively close together at pH 4, 5, and 6, respectively. Depending on the specific mineralogical composition of the clay fraction, SC is a suitable measure for the determination of dispersion properties and for the development of methods to keep clay particles in the soil in the flocculated state. 相似文献
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74.
Speciation in the parthenogenetic oribatid mite genus Tectocepheus (Acari, Oribatida) as indicated by molecular phylogeny 总被引:2,自引:0,他引:2
Michael Laumann Roy A. Norton Gerd Weigmann Stefan Scheu Mark Maraun Michael Heethoff 《Pedobiologia》2007,51(2):111-122
Despite the lack of universal concepts for species and speciation, both sexual and asexual organisms are expected to diversify into discrete genotypic and morphological clusters. Species-rich clusters of parthenogenetic oribatid mites likely evolved in the absence of sexual reproduction. We used nucleotide sequences of the large and small rDNA genes (18S and 28S) and the coding genes for heat shock protein 82 (hsp82) and elongation factor-1 alpha (ef-1α) for phylogenetic analyses of three morphotypes of the parthenogenetic oribatid mite genus Tectocepheus. DNA sequence divergences of the different morphotypes were similar to those of sexual species in other organisms. Maximum likelihood analyses of single genes and combined data sets were largely congruent in reconstructing the phylogeny of the Tectocepheus species. The results suggest that the different morphotypes of Tectocepheus indeed evolved in the absence of sexual reproduction, and that Tectocepheus minor, Tectocepheus velatus and Tectocepheus sarekensis are best considered distinct species. Further, the results suggest that 18S rDNA, hsp82 and ef-1α are powerful markers for resolving phylogenetic relationships of oribatid mites. Saturation plots indicated that the D3-region of 28S is much more saturated than all other examined genes. This indicates that the D3-region is unsuitable for resolving ancient splits in oribatid mites. 相似文献
75.
To explore long-term impact of organic and inorganic fertilizers on microbial communities, we targeted both the total bacterial community and the autotrophic ammonia oxidizing bacteria (AOB) in soil from six treatments at an experimental field site established in 1956: cattle manure, sewage sludge, Ca(NO3)2, (NH4)2SO4, unfertilized and unfertilized without crops. All plots, except the bare fallows, were cropped with maize. Effects on activity were assessed by measuring the basal respiration and substrate induced respiration (SIR) rates, and the potential activity of the AOB. To determine the bacterial community composition, 16S rRNA genes were used to fingerprint total soil communities by terminal restriction fragment length polymorphism analysis and AOB communities by denaturing gradient gel electrophoresis. The fertilization regimes had clear effects on both activity and composition of the soil communities. Basal respiration and r, which was kinetically derived as the exponentially growing fraction of the SIR-response, correlated well with the soil organic C content (r=0.93 and 0.66, respectively). Soil pH ranged from 3.97 to 6.26 in the treatments and was found to be an important factor influencing all microbial activities. pH correlated negatively with the ratio between basal respiration and SIR (r=0.90), indicating a decreased efficiency of heterotrophic microorganisms to convert organic carbon into microbial biomass in the most acid soils with pH 3.97 and 4.68 ((NH4)2SO4 and sewage sludge fertilized plots, respectively). The lowest SIR and ammonia oxidation rates were also found in these treatments. In addition, these treatments exhibited individually different community fingerprints, showing that pH affected the composition of AOB and total bacterial communities. The manure fertilized plots harbored the most diverse AOB community and the pattern was linked to a high potential ammonia oxidation activity. Thus, the AOB community composition appeared to be more strongly linked to the activity than the total bacterial communities were, likely explained by physiological differences in the populations present. 相似文献
76.
We investigated the structure of invertebrate food webs at three glacier foreland sites of an age of 2-34, ca. 60 and ca. 120 years in the European Alps at 2250-2450 m asl. The trophic structure was investigated by analyzing stable isotope ratios of 15N/14N and 13C/12C. The results suggest that the formation of terrestrial food webs during early primary succession heavily relies on prey out of the decomposer system with Collembola being most important. The diet of decomposers likely is based predominantly on allochthonous humus material blown in by wind and deposited by the retreating glacier. Irrespective of the successional stage the animal community consisted mainly of generalist predators with a number of species occurring at each of the successional stages. The results suggest that terrestrial food web formation is associated with a prolongation of food chains caused mainly by predator species switching their diet to include other predators, i.e. by intraguild predation. This suggests that generalist predators, such as cursorial spiders, carabid beetles, harvestman and centipedes, switch prey and include other predators if these are becoming more abundant, i.e. if ecosystems become more productive. Intraguild predation results in complex food webs with high linkage density which likely affects food web functioning and stability. 相似文献
77.
Stefan Ruyters Jelle Mertens Dirk Springael Erik Smolders 《Soil biology & biochemistry》2010,42(5):766-772
Field data have shown that soil nitrifying communities gradually adapt to zinc (Zn) after a single contamination event with reported adaptation times exceeding 1 year. It was hypothesized that this relatively slow adaptation relates to the restricted microbial diversity and low growth rate of the soil nitrifying community. This hypothesis was tested experimentally by recording adaptation rates under varying nitrification activities (assumed to affect growth rates) and by monitoring shifts in community composition. Soils were spiked at various Zn concentrations (0-4000 mg Zn kg−1) and two NH4+-N doses (N1, N2) were applied to stimulate growth. A control series receiving no extra NH4+-N was also included. Soils were incubated in pots under field conditions with free drainage. The pore water Zn concentration at which nitrification was halved (EC50, mg Zn l−1) did not change significantly during 12 months in the control series (without NH4+-N applications), although nitrification recovered after 12 months at the highest Zn dose only. The EC50 after 12 months incubation increased by more than a factor 10 with increasing NH4+-N dose (p < 0.05) illustrating that increased activity accelerates adaptation to Zn. Zinc tolerance tests confirmed the role of Zn exposure, time and NH4+-N dose on adaptation. Zinc tolerance development was ascribed to the AOB community since the AOB/AOA ratio (AOB = ammonia oxidizing bacteria; AOA = ammonia oxidizing archaea) increased from 0.4 in the control to 1.4 in the most tolerant community. Moreover, the AOB amoA DGGE profile changed during Zn adaptation whereas the AOA amoA DGGE profile remained unaffected. These data confirm the slow but pronounced adaptation of nitrifiers to Zn contamination. We showed that adaptation to Zn was accelerated at higher activity and was associated with a shift in soil AOB community that gradually dominated the nitrifying community. 相似文献
78.
Eleonora Flores-Ramírez Stefan Abel Thomas Nehls 《Soil Science and Plant Nutrition》2018,64(2):181-189
ABSTRACT Among potential components to construct Technosols for urban greening purposes, the commercially available geogenic coarse porous materials (CPMs) are mainly used in practice because of their high porosity. However, the knowledge of the hydraulic behavior of CPMs as well as of their mixtures with other substrates is limited, provoking their suboptimal usage. Therefore, we determined the water retention characteristics, including the available water capacity (AWC) of six geogenic CPMs: porlith, expanded shale, expanded clay, tuff, pumice, and lava. In order to obtain the water retention characteristics of the CPMs as well as of their mixture with sand (1:4 per volume), the following methods adapted from soil physics were applied over a wide range of pressure heads: Equi-pF apparatus, ceramic tension plates, pressure plate extractors, WP4C apparatus, and water vapor adsorption. The results were used to parametrize the modified Kosugi model (using SHYPFIT 2.0). Porlith and tuff have the highest AWC (0.37 m3 m?3 and 0.17 m3 m?3, respectively) and are the only ones which can be recommended as effective water-retaining materials. Further materials exhibit an AWC less than 0.10 m3 m?3. The CPMs exhibit a bimodal pore size distribution, which can be well described by the applied model, except for pumice and expanded shale. The mixtures present overall low AWCs up to 0.07 m3 m?3, with the pure sand having less than 0.03 m3 m?3. For practical application a quite high ratio of CPM is needed, and the mixing material must be adapted to the hydraulic properties of the CPMs. The water inside the CPMs may be easily available for plant roots able to penetrate in the CPMs’ coarse pores. 相似文献
79.
Ruminants, which have a central place in the food chain, ingest soil that may contain pollutants. The bioaccessibility of three different polycyclic aromatic hydrocarbon compounds from soil was studied using an in vitro model based on the digestive tract of cows. For this purpose, pasture soil was spiked with (14)C radio-labeled compounds, aged, and then exposed to conditions which simulated the digestive compartments of the rumen, abomasum, and intestines. Our results show that aging generally reduced the bioaccessibility of all the compounds tested. Total bioaccessibilty in the first digestive compartment, i.e., the rumen, depended on the considered compound: elevated for phenanthrene (17-24%), moderate for pyrene (6.6-8.1%), and low for benzo[ a]pyrene (2.3-3.6%). Bioaccessibility was very low in abomasal acidity (generally <2%) and intestinal colloids (<8%). The liquid phases of intestinal medium successfully extracted compounds from freshly contaminated soil (25-28%), but the bioaccessibilty dropped markedly after aging (17% for phenanthrene and <9% for the more lipophylic compounds). Total bioaccessibilty in this in vitro model ranged from 11% for benzo[ a]pyrene in aged soil to 58% for phenanthrene in freshly contaminated soil, and the bioaccessibility of this latter compound was always higher compared to pyrene or benzo[ a]pyrene. Residual soil contained around half of the initial load, the highest residual levels being of benzo[ a]pyrene, which confirms the observed bioaccessibility. 相似文献
80.
Bergeron C Gafner S Clausen E Carrier DJ 《Journal of agricultural and food chemistry》2005,53(8):3076-3080
The aqueous extract of American skullcap (Scutellaria lateriflora L. (S. lateriflora), Lamiaceae) has been traditionally used by North American Indians as a nerve tonic and for its sedative and diuretic properties. Recent reports stated that flavonoids and possibly amino acids are responsible for the anxiolytic activity. As a part of our search for environmentally friendly solvents to extract the active components from medicinal plants, we used S. lateriflora in a comparison of accelerated solvent extraction (ASE) using water, and supercritical fluid extraction (SFE) using CO2 and 10% EtOH as modifier, at different temperatures. Flavonoids and amino acids were quantified by HPLC-UV and HPLC-MS, respectively. The flavonoid content was compared with conventional extraction methods (hot water extraction and 70% ethanol). The use of ASE at 85 degrees C with water as solvent gave the best results for flavonoid glycosides and amino acids, whereas SFE gave higher yields of flavonoid aglycones. However, the results obtained for total flavonoids were not significatively superior to hot water extraction or 70% aqueous EtOH extract. 相似文献