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941.
The nucleoporins Nup58 and Nup45 are part of the central transport channel of the nuclear pore complex, which is thought to have a flexible diameter. In the crystal structure of an alpha-helical region of mammalian Nup58/45, we identified distinct tetramers, each consisting of two antiparallel hairpin dimers. The intradimeric interface is hydrophobic, whereas dimer-dimer association occurs through large hydrophilic residues. These residues are laterally displaced in various tetramer conformations, which suggests an intermolecular sliding by 11 angstroms. We propose that circumferential sliding plays a role in adjusting the diameter of the central transport channel. 相似文献
942.
Chiu DT Wilson CF Ryttsén F Strömberg A Farre C Karlsson A Nordholm S Gaggar A Modi BP Moscho A Garza-López RA Orwar O Zare RN 《Science (New York, N.Y.)》1999,283(5409):1892-1895
Individual phospholipid vesicles, 1 to 5 micrometers in diameter, containing a single reagent or a complete reaction system, were immobilized with an infrared laser optical trap or by adhesion to modified borosilicate glass surfaces. Chemical transformations were initiated either by electroporation or by electrofusion, in each case through application of a short (10-microsecond), intense (20 to 50 kilovolts per centimeter) electric pulse delivered across ultramicroelectrodes. Product formation was monitored by far-field laser fluorescence microscopy. The ultrasmall characteristic of this reaction volume led to rapid diffusional mixing that permits the study of fast chemical kinetics. This technique is also well suited for the study of reaction dynamics of biological molecules within lipid-enclosed nanoenvironments that mimic cell membranes. 相似文献
943.
Chromosomal localization of muscle nicotinic acetylcholine receptor genes in the mouse 总被引:9,自引:0,他引:9
O Heidmann A Buonanno B Geoffroy B Robert J L Guénet J P Merlie J P Changeux 《Science (New York, N.Y.)》1986,234(4778):866-868
The chromosomal localization of the genes encoding the four subunits of muscle nicotinic receptor was determined by analyzing restriction fragment length polymorphisms between two mouse species Mus musculus domesticus (DBA/2) and Mus spretus (SPE). Analysis of the progeny of the interspecies mouse backcross (DBA/2 X SPE) X DBA/2 showed that the alpha-subunit gene cosegregates with the alpha-cardiac actin gene on chromosome 17, that the beta-subunit gene is located on chromosome 11, and that the gamma- and delta-subunit genes cosegregate and are located on chromosome 1. 相似文献
944.
Boly et al. (Reports, 13 May 2011, p. 858) investigated cortical connectivity patterns in patients suffering from a disorder of consciousness, using electroencephalography in an auditory oddball paradigm. We point to several inconsistencies in their data, including a failure to replicate the classical mismatch negativity. Data quality, source reconstruction, and statistics would need to be improved to support their conclusions. 相似文献
945.
The study of past forest change provides a necessary historical context for evaluating the outcome of human-induced climate change and biological invasions. Retrospective analyses based on fossil and genetic data greatly advance our understanding of tree colonization, adaptation, and extinction in response to past climatic change. For instance, these analyses reveal cryptic refugia near or north of continental ice sheets, leading to reevaluation of postglacial tree migration rates. Species extinctions appear to have occurred primarily during periods of high climatic variability. Transoceanic dispersal and colonization in the tropics were widespread at geological time scales, inconsistent with the idea that tropical forests are particularly resistant to biological invasions. 相似文献
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948.
Digital elevation models of the Northern and Southern Patagonia Icefields of South America generated from the 2000 Shuttle Radar Topography Mission were compared with earlier cartography to estimate the volume change of the largest 63 glaciers. During the period 1968/1975-2000, these glaciers lost ice at a rate equivalent to a sea level rise of 0.042 +/- 0.002 millimeters per year. In the more recent years 1995-2000, average ice thinning rates have more than doubled to an equivalent sea level rise of 0.105 +/- 0.011 millimeters per year. The glaciers are thinning more quickly than can be explained by warmer air temperatures and decreased precipitation, and their contribution to sea level per unit area is larger than that of Alaska glaciers. 相似文献
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