Proliferation of disease pathogens capable of affecting humans, domestic livestock and wildlife increasingly threatens environmental
security and biodiversity. Livestock and wild animals in proximity to human beings are often in the chain of transmission
and infection. Globalization of industrial livestock production (especially poultry upon which so much of the burgeoning human
population depends) often permits transcontinental disease spread. Rapidly expanding (and often illegal) international trade
in wild and domestic animals and their products are increasingly involved in the emergence of new diseases that may have the
ability to transmit among humans, livestock and wildlife. Rapidly increasing urbanization has led in many places to overcrowded
townships that rely on “bushmeat” for sustenance and has contributed to the emergence of virulent zoonotic pathogens. The
emergence and proliferation of pathogens are exacerbated by anthropogenic transformation of natural landscapes in order to
increase agricultural and livestock production. This paper posits that data gathered by veterinary ecologists should be interpreted
and used by other disciplines. The importance of a thorough knowledge of the “natural history” (ecology) of the disease agent
and its human, domestic and wild hosts is stressed. 相似文献
The purpose of this study was to evaluate the effect of withdrawal of lactose from the diet for 72 hours on lactase activity in the jejunal mucosa of conventionally raised calves. The descending portion of the duodenum of six Holstein calves less than 24 hours old was cannulated. The calves were fed milk except on days 5, 6 and 7 when they were given the same volume of an electrolyte solution. Sequential biopsy specimens of the proximal jejunal mucosa were obtained for three weeks and the lactase activity determined. Lactase activity was highest on day 1 and a trend toward decreased lactase activity from birth until three weeks was observed. Mean lactase activity was significantly (p less than 0.05) higher for days 1, and 3 compared to days 9, 13 and 17. The withdrawal of milk and replacement by an electrolyte solution during three days had no significant effect on jejunal mucosal lactase activity in neonatal calves. 相似文献
A hepatic mass was identified in a 5-year-old, female mixed-breed cat that died spontaneously after a clinical history of progressive emaciation, ptyalism, and persistent coryza. At necropsy, a 7-cm-diameter, yellow-brown, firm, multilobulated tumor was identified in the liver. Microscopically, the mass consisted of neoplastic cells arranged in small, closely packed nests within a thin fibrovascular stroma. These cells were of medium sized and polygonal, with fine argyrophilic cytoplasmic granules. Nuclei were predominantly round with finely stippled chromatin and indistinct nucleoli. Mitotic figures were numerous. Immunohistochemically, most of the neoplastic cells were immunoreactive for chromogranin A, neuron-specific enolase (NSE), and cytokeratin AE1/AE3 and weakly labeled for synaptophysin. The tumor was negative for glial fibrillary acidic protein (GFAP), vimentin, and cytokeratins 5, 6, 8, and 17. Vascular emboli and intrahepatic micrometastasis were also identified with chromogranin A. All these features were consistent with a hepatic neuroendocrine carcinoma and emphasized the importance of using a panel of antibodies to diagnose such rare tumors. 相似文献
1. The aim of this study was to evaluate the occurrence of Campylobacter spp. in domestic and free-living pigeons and to evaluate the antibiotic resistance profiles.
2. The material consisted of cloacal swabs obtained from 108 homing pigeons and fresh faeces from 72 wild birds from Lublin and its vicinity. The identification of strains isolated on differential/selective media for Campylobacter spp. was carried out by MALDI-TOF and PCR. The susceptibility to antibiotics was evaluated by minimum inhibitory concentration (MIC) in Mueller-Hinton broth.
3. A total of 35 strains of Campylobacter spp. were isolated; 27 were identified as Campylobacter jejuni and 8 as Campylobacter coli. Over half of the isolates were resistant to erythromycin and streptomycin, 40% of strains were resistant to tetracycline and ampicillin and 37% isolates were resistant to amoxicillin. Resistance to two or more antibiotics was observed in all strains tested.
4. The results indicate that both domestic and free-living pigeons are reservoirs for bacteria of the genus Campylobacter, which are characterised by varied and growing resistance to commonly used antibiotics. 相似文献
Research in control of tick-borne diseases and trypanosomosis, and their vectors, namely, ticks and tsetse flies respectively,
has been on going for decades. However, very little attention has been paid to the socio-economic factors that are likely
to influence the outcome of the interventions in the control of these diseases. Thus, this study was designed to investigate
these factors, mainly the intra-household factors influencing decision-making in the control of Vector-borne diseases in the
pastoralist areas of Uganda. These factors included: indigenous technical knowledge, household economic factors, and gender.
Both qualitative and quantitative methods were used in the collection and analysis of data. The tools used for data collection
included among others, participatory learning and action (PLA), and Case studies. The findings included the following: In
pastoralist households, a big proportion of the household budget was allocated to vector-borne diseases control. In the male-headed
households, men dominated decision-making on vector-borne diseases control, although the goals and priorities of men and women
in these households were not the same. Also, vector-borne disease control was predominantly by use of modern veterinary drugs,
and pastoralists treated sick cattle by themselves even in situations where there were veterinary personnel. 相似文献
Pituitary pars intermedia dysfunction is the most prevalent endocrine disease in horses. Although donkeys and mules may also be affected, only a few data have been published. Reference values for diagnostic parameters, such as adrenocorticotropic hormone (ACTH), are especially scarce or even lacking. Therefore, in the present study, available data from the literature have been verified and completed to facilitate a reliable diagnosis. Clinical inspections and haematological and biochemical examinations were carried out four times in a three-month interval (February to November) in 44 donkeys and 31 mules. Data from clinically healthy animals were used as an orientation. Plasma ACTH concentrations showed seasonal changes in both animal groups. However, it was generally higher in donkeys than mules. Although blood glucose (EDTA plasma) showed no difference between groups, serum insulin concentrations were consistently higher in donkeys. Serum fructosamine levels were slightly higher in mules, whereas, in some cases, serum triglyceride levels were considerably higher in donkeys. Serum gamma-glutamyltransferase showed a striking peak in mules in August, whereas the remaining gamma-glutamyltransferase values were lower compared to donkeys. By comparing donkeys and mules, the present work reveals differences in various blood parameters which should be considered for diagnoses and future studies. 相似文献
The compatibility and protectant potential of Calneem® oil derived from the neem tree Azadirachta indica and two parasitoids, Habrobracon hebetor and Venturia canescens, for the control of the rice moth Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae) and the tropical warehouse moth Cadra cautella Walker (Lepidoptera: Pyralidae) in stored rice and wheat, were evaluated in the laboratory. Calneem® oil (= neem oil) is a biopesticide produced, registered and marketed in Ghana by AQUA AGRIC Community Projects (AACP/Caldor Ghana Ltd., Tema). It contains 0.3% azadirachtin as its major active ingredient. The oil was emulsified with water using 0.07% soap. Fourth instar moth larvae were held in grain treated with neem oil only, grain treated with one of the parasitoids only, grain treated with a combination of neem and one of the parasitoids, and a control with untreated grain. Neem oil was applied at concentrations from 5,000 to 30,000 ppm. All samples were kept in growth cabinets maintained at 25°C and 65–70% r.h. Adult emergence was recorded after 4 weeks. Parasitoid or neem treatments alone reduced the emergence of C. cephalonica and C. cautella. In general, parasitoid releases were as effective as a combination of neem oil and parasitoids. At the lowest dose, 5,000 ppm, the combination of neem and parasitoid was more effective than the neem alone. The number of adults of H. hebetor and V. canescens that emerged in rice containing either parasitoids alone or in combination with neem oil was similar. This indicates minimal or no adverse effect of neem oil on the two parasitoids. It is thus possible to incorporate neem oil in a well-designed pest management program with parasitoids. 相似文献
Strains of the Japanese pear pathotype of Alternaria alternata were screened for double-stranded RNAs (dsRNAs). Four strains had several dsRNAs; strain N18 was associated with several
dsRNAs and had impaired growth phenotypes such as irregular mycelium and abnormal pigmentation. We isolated dsRNA-cured isolates
from strain N18 by single-conidium isolation. The dsRNA-cured isolates had recovered normal growth and pigmentation. Enlarged
vesicles were observed in mycelial cells of the original dsRNA-carrying N18 strain. DAPI nuclear staining revealed regression
of the nuclei in dsRNA-carrying N18 cells. These results indicate that the dsRNAs might have negative effects, such as apoptosis-like
cell death, on the host fungus. 相似文献