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21.
Kim KH Choi SU Choi SZ Son MW Lee KR 《Journal of agricultural and food chemistry》2011,59(13):6980-6984
Edible yams are tropical crops that serve as important staple foods in many parts of the world. The rhizome of Dioscorea japonica , well-known as "Japanese yam", is a food and medicinal source known as "San Yak" in Korea. Bioassay-guided fractionation and chemical investigation of the extract of this yam resulted in the identification of two new withanolides, named dioscorolide A (1) and dioscorolide B (2). The structures of these new compounds were determined by spectroscopic methods, including 1D and 2D nuclear magnetic resonance (NMR) techniques, high-resolution mass spectrometry (HRMS), and chemical methods. The cytotoxic activities of the isolates (1 and 2) were evaluated by determining their inhibitory effects on four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and HCT15) and a human normal cell line (HUVEC) using a sulforhodamine B (SRB) bioassay. Compounds 1 and 2 showed cytotoxicity against tumor cell lines (A549, SK-OV-3, SK-MEL-2, and HCT15) with IC(50) values ranging from 6.3 to 26.9 μM and exhibited lower activity against the normal cell line (HUVEC) with IC(50) values ranging from 27.1 to 28.8 μM, suggesting selective toxicity among tumor and normal cells. 相似文献
22.
Studies were conducted during 4 months of each growing season in 1994 and 1995 to measure water use of young apple trees (Malus domestica Borkh. cv ‘Fuji’) growing under different soil moisture regimes in temperate climate conditions and to evaluate monthly crop coefficients of such conditions. To do so, double pot lysimeters under a transparent rain shield were designed and installed. The three soil moisture regimes in three replicates each were: (A) drip-irrigation at −50 kPa of soil matric potential (IR50); (B) drip-irrigation at −80 kPa of soil matric potential (IR80); and (C) constant shallow water table at 0.45 m below the soil surface (WT45). In each treatment, soil surface was maintained with or without turf grasses. Monthly water use was not different in drip-irrigated treatments (IR50 and IR80), but greatest in the WT45 treatment. Monthly crop coefficients increased linearly in time for drip-irrigated apple trees (r2 values of 0.76*** for IR50 and of 0.77*** for IR80), while those obtained in the WT45 treatment fluctuated. Leaf water potential (LWP) of drip-irrigated trees was similar until 63 days after treatment (DAT), but the values for IR80 trees began to decline thereafter. The LWP of WT45 trees decreased from 48 DAT. Temporal variations in leaf water content (LWC) was similar to that of LWP, except for two abrupt decreases in IR80 trees. The LWC of WT45 trees began to decrease from 59 DAT, and this occurred 2 weeks after the reduction in LWP. Average shoot length of IR50 trees was greater than that of IR80 and WT45 trees. The results of this study provided water use and crop coefficients for apple trees in relation to soil moisture regimes under temperate climate. 相似文献
23.
Summary 2-Chloroethylphosphonic acid (CEPA) was applied, as a foliar spray in the form of Ethrel, to potato plants (var. Uran). The
effects on water loss, chlorophyll degradation, and on the growth potential of tubers from the treated plants, were studied
and compared with the effects of diquat.
At concentrations of 20 and 10 g per kg CEPA brought about desiccation comparable to the effect of diquat: the effect on chlorophyll
degradation was somewhat slower. Growth potential of the tubers, measured as the rate of emergence during the next vegetation
period, was not affected by any treatment. 相似文献
24.
Doo-Won Song Woong-Bin Ro Hee-Myung Park 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(5)
BackgroundProgrammed cell death protein-1 (PD-1) and programmed cell death ligand-1 (PD-L1) have important roles in tumor evasion of the immune system.ObjectivesThis study aimed to assess the diagnostic utility of circulating PD-1 and PD-L1 levels in healthy dogs and dogs with tumors.MethodsCirculating PD-1 and PD-L1 levels in the serum of 71 dogs with tumors were compared with those of 52 healthy dogs by performing enzyme-linked immunosorbent assay (ELISA).ResultsThe ELISA results revealed higher circulating PD-1 and PD-L1 levels in dogs with tumors (2.9 [2.2–3.7] ng/mL; median [IQR] and 2.4 [1.4–4.4] ng/mL, respectively) than in healthy dogs (2.4 [1.9–3.0] ng/mL; p = 0.012 and 1.4 [0.9–2.1] ng/mL; p < 0.001, respectively). Especially, there was a significant difference in circulating PD-1 levels between healthy dogs and dogs with malignant epithelial tumors (2.4 [1.9–3.0] ng/mL and 3.1 [2.6–4.4] ng/mL, respectively; p < 0.01). In addition, there was a significant difference in circulating PD-L1 levels between healthy dogs and dogs with lymphomas (1.4 [0.9–2.1] ng/mL and 2.7 [1.6–5.8] ng/mL, respectively; p < 0.001).ConclusionThis study indicates that circulating PD-1 and PD-L1 have potential as tumor diagnostic biomarkers in dogs with tumors. 相似文献
25.
Guillermo Riesco Muñoz María A. Soilán Cañas Roque Roíguez Soalleiro 《Annals of Forest Science》2008,65(5):507-507
26.
Dietmar Roßberg Silke Dachbrodt-Saaydeh Jan Helbig Madeleine Paap Hella Kehlenbeck 《Gesunde Pflanzen》2018,70(3):139-146
The Institute for Strategies and Technology Assessment of the Julius Kühn-Institute operates four different networks: demonstration farms on IPM, reference farms for plant protection, panel pesticide applications and NEPTUN farms. All networks deal with the use of plant protection products in agricultural practice. The latter three also have very similar tasks and are closely interlinked. As a result, outsiders have significant problems distinguishing between those four farm networks and assigning the respective activities to the “right” corresponding network.Therefore, the publication aims at presenting comparatively the tasks and objectives of the various networks and to explain to the reader what are they doing, what are the differences, as well as why and how the establishment of these four different farm networks has come about. 相似文献
27.
Freely available information on the actual use of chemical plant protection products (PPPs) in agriculture is highly necessary for a number of scientific questions and political discussions. Therefore, since 2000, regular surveys on the use of PPPs have been carried out for the most important agricultural and horticultural crops in Germany (NEPTUN projects). In 2011, they were adjusted to legal framework changes. Since then they are known as PAPA surveys with “PAPA” being an abbreviation for Panel Pesticide Applications. For each crop a network of farms was built up. In each network, the PPP application data are collected annually, anonymized and forwarded to the Julius Kühn-Institute (JKI).All surveys and analyses based on the panel refer to the Federal Republic of Germany. The participating farms are distributed throughout Germany proportionally to the production area per crop.In sugar beet cultivation the results of PAPA surveys show that the plant protection intensity has increased slightly in recent years comparing the PAPA results with the years 2005 to 2010. There are diverse reasons for this development. Difficult-to-control weeds occurred on an increasing acreage in recent years. At the same time, an early appearance of foliar diseases (approximately beginning of July) combined with warm and humid weather during the following weeks leads to the development of the main pathogen causing leaf spot diseases in sugar beet (Cercospora beticola) in many regions. A continuing development of leaf spots increases the need for repeated fungicide applications. A high infestation with aphids was the dominating reason for increased insecticide applications in some years. Additional influences on the treatment index are due to changes in the authorization of PPPs. 相似文献
28.
The article describes a novel analytical method based on solid-phase extraction and LC-MS/MS for detection of a high number (n?=?44) of selected pharmaceuticals in surface waters. The validated method showed suitable accuracy, precision, and linearity. The extraction efficiency for pharmaceuticals was mostly above 90% and the determined LOQs were generally below 5 ng/L and even down to 0.03 ng/L. The matrix effect was successfully minimized with isotopically labeled internal standards. Six river and lake samples from Slovene regions were afterwards analyzed. Forty-two of the 44 target pharmaceuticals were detected in at least two water samples; 66% of them were detected in all samples. Three analytes were present above the LOQ in all samples: caffeine (1.1–3.1 ng/L), irbesartan (0.2–9.3 ng/L), and valsartan (0.8–47 ng/L). The highest concentration was measured for valsartan. Comprehensive comparison with the literature data showed that the found concentrations of pharmaceuticals were notably lower in our study and that a number of the pharmaceuticals had not been reported previously. Comparison of the measured and predicted (calculated) environmental concentrations of pharmaceuticals in surface waters showed high discrepancy between the two approaches, clearly indicating the need for comprehensive analytical methods for routine monitoring of these newly emerging pollutants. In conclusion, our method was proven to be adequately sensitive to reliably quantify the majority of pharmaceutical contaminants even though they were found in notably lower concentrations in Slovenian surface waters than it was predicted from their consumption and from the literature. 相似文献
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