制备植物病毒、细菌抗血清的连续采血法研究和应用

用植物病毒、细菌抗源(加福氏完全佐剂)免疫家兔,从末次免疫后7天开始,1—5个月内进行耳静脉连续采血,1周2次,能获得比心脏采血多15倍的抗血清。耳静脉抗血清用于酶联免疫吸附试验、免疫电镜、反向间接血凝、乳胶凝集试验、免疫双扩散、对流免疫电泳技术检测抗原,均得到很好的结果。     

武定鸡早期肉用性能遗传规律的研究

 本文对武定鸡90日龄活重、胸角、龙骨长及屠体性状的遗传规律初步进行了探讨。材料取自我校鸡场1985年1～6月两批出雏的各200只纯繁殖鸡种,测定诸性状的平均数、标准差。遗传分析用单元内半同胞相关法,将不同季节及性别不同资料合并,计算活重遗传力为0.364,龙骨0.1843,胸角0.376,屠体十个性状的遗传力最高是全净膛重0.8748,最低屠体重0.1,此外着重分析活重、龙骨长、胸角度与屠体主要性状的表型相关、遗传相关及环境相关。结果活重与胸角度、龙骨长间存在较高的遗传相关(0.425、0.4652)。而这三个性状,又与屠体诸性状的主要性状间呈较强的正相关。因此通过对活重等三个性状的辅助选择,有可能提高选种的准确性,从而提高遗传改进量。     

罗非鱼在不同盐度水体中的生长繁殖和鱼肉粗蛋白与氨基酸含量的比较

罗非鱼原产非洲淡水中,但越来越多的人认为其祖先起源于海洋,因为多数种具有耐盐能力,尼罗罗非鱼就是其中之一。本试验的目的在于观察尼罗罗非鱼在海水中养殖的可行性。 材料与方法 实验鱼取自实验室内同胎孵出的25天龄尼罗罗非鱼(Oreochromis niloticus)鱼苗99尾,实验分为淡水、咸淡水(1/2海水+1/2淡水)和海水三个饲养组,每组33尾鱼苗,平均个体重0.15克,分别养于三个容量相同(内径80厘米,高60厘米)的白色圆形玻璃钢水槽中。控温26℃±1℃),充气,投喂豆饼、浒苔和白菜。每周换水1—2次。每个月月底称量各组鱼之总重和计算存活鱼尾数。实验始于1983     

压燃机燃用醇-柴混溶燃料的性能研究

乙醇是一种从植物、农业废弃物等转化的再生性能源。应用这种能源的深远意义在于:农业生产者本身也可以成为非石油制品燃料的生产者。 本文详细阐明了压燃机燃用醇—柴混溶燃料所具有的动力性、经济性、燃烧过程、排放烟度、低速扭矩特性、工作噪声等以及改善这些特性的方法与效果。 试验表明:通过合理的调整(供油体积和喷油提前角)和进气温度的控制,未经任何改装S—195型压燃机可以燃用含醇量为17％的混溶燃料,并获得与燃用纯柴油相当的性能指标。     

坡面流速的试验研究

本文首先对于现有各种坡面流速公式作一简单概述,进而简介坡面流速的试验布置。根据不稳定流计算理论,对试验资料进行了整理分析,得到下列的坡面流速公式:V=kq^0.5I^0.35式中:q——单宽流量（立方厘米/秒·米）;I——坡面的坡度比值;V——相应于q和I的坡面流速（厘米/秒）;k——系数。是随坡面的表面特征而异,在本试验裸地条件下k为2.0。然后,在试验给定的流态和公式的基础上,结合应用现有坡面流速公式,反求k值。按地表特征分类归纳,取其平均值为采用值。     

斑节对虾Toll9受体基因免疫功能的研究与表达分析

Toll受体蛋白(Toll receptors)是一类重要的模式识别受体,在无脊椎动物先天性免疫系统中发挥重要作用。文章对斑节对虾(Penaeus monodon)新型Toll9受体基因(Pm Toll9)进行了研究:以人源胚胎肾细胞(HEK293T)成功构建体外细胞免疫模型,通过免疫印迹方法证实Pm Toll9重组真核蛋白可在HEK293T中成功表达。双荧光素酶报告系统检测发现在200 ng转染浓度处Pm Toll9对NF-κB报告基因的激活效果显著。q RT-PCR数据证明PmToll9成功激活HEK293T细胞Toll-like receptor(TLR)信号通路,促进通路下游髓样分化因子(My D88)、肿瘤坏死因子(TNF-α)和白介素(IL-10)的上调表达,同时酶联免疫吸附测定结果证明Pm Toll9可促进TNF-α蛋白表达水平显著上调。斑节对虾体内细菌刺激实验结果显示无乳链球菌(Streptococcus agalactiae)可激活Pm Toll9在肝胰腺、肠、淋巴和鳃中的表达,哈维氏弧菌(Vibrio harveyi)可显著抑制Pm Toll9在肝胰腺中的表达。提示无乳链球菌可通过Pm Toll9激活Toll信号通路,引起机体免疫防御反应,而哈维氏弧菌对此过程具有一定的抑制作用。     

喷杆式喷雾机雾流方向角对药液沉积影响的试验研究

为了研究喷杆式喷雾机的雾流方向角对靶标上药液沉积量的影响,设置±40°、±30°、±20°、±10°、0° 9个雾流方向角,选用标准扇形雾喷头ST120-015,喷雾压力0.25 MPa,分别以1.18 m/s、0.59 m/s的前进速度,按照150 L/hm²和300 L/hm²的喷量针对水平靶标和垂直靶标进行喷雾试验。试验结果表明,改变雾流方向角会增加药液在水平靶标上的沉积量,药液在中部和下部靶标沉积量的增加程度比上部显著,喷量150 L/hm²和300 L/hm²的最佳喷雾雾流方向角分别为20°和30°;对于垂直靶标,喷量150 L/hm²时改变雾流方向角只会增加上部和下部靶标的药液沉积量,而喷量300 L/hm²时改变雾流方向角会使上、中、下三部分靶标的沉积量都增加,且增加程度一致。当喷量150 L/hm²时垂直靶标的最佳雾流方向角为20°～40°,喷量300 L/hm²时的最佳雾流方向角为30°。试验结果表明喷杆前进速度会对最佳雾流方向角产生影响。     

Study on biological and ecological characteristics ofIps subelongatus Motsch

Ips subelongatus Motschulsky were raised indoors and in the field using fresh baits for study of biological and ecological characteristics during 1992–1994. The life cycle of the pest in Lishu County, Jilin Province was described. The study results indicated that temperature was a main factor affecting the development ofIps subelongatus. The initial temperature for development of egg, larva, pupa and adult was 6.1, 8.0, 10.3 and 9.8°C, respectively. To complete one-generation needs, respectively, 48 d at temperature of 18°C, 39 d at 20°C and 33 d at 22°C. The pest has different number of generations in different area as a result of temperature change. In Jilin Province, it generally has three generations, except that some individuals have 2 generations. The nature enemies for this pest areThanasinus substriatus, Tomimicobia seitneri Ruschk,Coeliodes sp. etc. Foundation item: This paper is part of key subject of the Ministry of Forestry (formerly)—The Systematic Control Technique ofIps subelongatus Motschulsky. Biography: G{upao} Chang-qi (1952-), male, research fellow in Jilin Forestry Academy. Responsibe editor: Chai Ruihai     

Pharmacokinetic study of baicalein after oral administration in monkeys

Baicalein, a flavonoid originally isolated from the root of Scutellaria baicalensis Georgi, has numerous pharmacological activities. Up to now, several studies regarding the pharmacokinetic profiles of baicalein have been described, while there is no such study reported in monkey, the species which is more similar to human. The purpose of this study was to investigate the pharmacokinetic profiles of baicalein after oral administration in monkeys. After orally administrating three doses of baicalein in monkeys, multi-peaks of the plasma concentration-time curves were observed and the non-linear pharmacokinetics for baicalein and its metabolite baicalin were found at doses of 50-500mg/kg. In order to calculate the absolute bioavailability, the intravenous pharmacokinetic study was also carried out after intravenous administration of 10mg/kg baicalein. The absolute bioavailability of baicalein in different doses was ranged from 13.1% to 23.0%. In this study, baicalein and baicalin were determined by LC-MS method. The chromatographic separation was performed on Agilent Poroshell 120 SB-C18 column (2.7μm, 2.1×50mm). Baicalein and baicalin were detected by single quadrupole mass spectrometer equipment with electrospray ionization interface with the selected ion monitoring mode. The assay was linear for both baicalein and baicalin with the correlation coefficients>0.99. The intra- and inter-day precisions for baicalein and baicalin were all less than 15% by relative standard deviation. The analytes were stable during samples storage and handling, and no matrix effects were observed. The method we developed in this study was sensitive, precise, stable and producible.