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21.
The effect of exploratory laparotomy on the serum and peritoneal haptoglobin concentrations of the pony. 总被引:1,自引:0,他引:1 下载免费PDF全文
Serum haptoglobin concentration was used as an indicator of the acute phase response in ponies undergoing exploratory laparotomy. Preoperative, 1 h intraoperative, 3 h, 6 h, 12 h and 24 h postoperative blood samples and 48 h postoperative peritoneal fluid samples were obtained for haptoglobin analysis. A spectrophotometric assay based on cyanmethemoglobin binding capacity (CyanBC) was used to determine haptoglobin concentrations. The preoperative reference range for serum haptoglobin concentrations in these ponies was 25-60 mg CyanBC/dL. Intraoperative and 3 h postoperative blood samples had decreased haptoglobin concentrations when compared to preoperative values. Serum haptoglobin concentrations began to rise by the 6 h postoperative sample and were generally elevated above preoperative values by the 24 h postoperative sample. Two of the ten ponies had mild signs of postoperative colic which were associated with twofold elevations in serum haptoglobin concentrations and fivefold elevations in peritoneal fluid haptoglobin concentrations. 相似文献
22.
J Tetens S A Barker M Waguespack G Hosgood 《Canadian journal of veterinary research》2001,65(2):133-135
The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period. 相似文献
23.
Transmission of porcine reproductive and respiratory syndrome virus from persistently infected sows to contact controls. 总被引:3,自引:0,他引:3 下载免费PDF全文
M D Bierk S A Dee K D Rossow S Otake J E Collins T W Molitor 《Canadian journal of veterinary research》2001,65(4):261-266
The objective of this study was to determine if porcine reproductive and respiratory syndrome virus (PRRSV) could persist in non-pregnant sows and if persistently infected sows could transmit virus to naive contact controls. Twelve PRRSV-naive, non-pregnant sows (index sows) were infected with a field isolate of PRRSV and housed in individual isolation rooms for 42 to 56 days postinfection. Following this period, 1 naive contact sow was placed in each room divided by a gate allowing nose-to-nose contact with a single index sow. Index sows were not viremic at the time of contact sow entry. Virus nucleic acid was detected by polymerase chain reaction, and infectious virus was detected by virus isolation in sera from 3 of the 12 contact sows at 49, 56, and 86 days postinfection. All 3 infected contacts developed PRRSV antibodies. Virus nucleic acid was detected in tissues of all of the 12 index sows at 72 or 86 days postinfection. Nucleic acid sequencing indicated that representative samples from index and infected contacts were homologous (> 99%) to the PRRSV used to infect index sows at the onset of the study. This study demonstrates that PRRSV can persist in sows and that persistently infected sows can transmit virus to naive contact animals. 相似文献
24.
提高真菌植酸酶热稳定性的方法 总被引:2,自引:0,他引:2
植酸酶(Phytase)能提高饲料中磷及其他养分的利用率,减少粪便中磷的排泄量,同时还能降低植酸(盐)的抗营养作用,在饲料中添加植酸酶对提高畜禽生产性能及减轻环境污染有着重要意义。真菌植酸酶具有pH作用范围广、活性高和易提纯等优点,是生产植酸酶的最佳来源。但是真菌植酸酶热稳定性普遍较低,不能完全满足饲料加工的要求。本文综述了在提高真菌植酸酶热稳定性研究方面的最新进展,讨论了其进一步的研究方向。 相似文献
25.
当今社会科技日新月异,推动着社会文明进程和经济的快速发展,全球犬业呈现出蓬勃生机。纵观国内外犬业发展史,大多以优良品种、训用技术技能、器具研发为犬业的三大支柱,三方面强则犬业兴。笔者现就发展我国犬业的思考抛砖引玉,愿与犬业界有识之士共商。 相似文献
26.
T D Butt A M Cruz J V Bailey W H Crawford 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(12):936-939
The object of this investigation was to determine the outcome of limb amputation in wapiti. Medical records of 13 wapiti that underwent limb amputation were reviewed to determine age, weight, sex, injury preceding amputation, limb amputated, amputation location, length of hospital stay, complications, and outcome. Ages ranged from 3 months to 8 years and weights from 70 kg to 280 kg. All animals were female. Eleven animals sustained catastrophic long bone fractures prior to amputation. Five animals had front limb amputations and 8 had hind limb amputations. Of these animals, 1 with a front limb amputaiton and 5 with hind limb amputations survived. Postsurgical inability to stand is associated with hospitalization of > 1 day. Of the 13 wapiti reviewed, 5 with hind limb amputations have produced and reared offspring. Hind limb amputation can be considered a viable alternative to euthanasia when catastrophic orthopedic injuries occur in female wapiti. 相似文献
27.
当压缩机内气体的流量较低时,气体容易出现旋转脱离现象,导致压缩机不能正常工作,采取增加压缩机内部液体的流量,在出口处设置单向阀防止气体倒流等措施,可有效地防止喘振的发生,保证输气管道的正常运行. 相似文献
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