全文获取类型
收费全文 | 1602篇 |
免费 | 120篇 |
国内免费 | 4篇 |
专业分类
林业 | 95篇 |
农学 | 40篇 |
基础科学 | 7篇 |
200篇 | |
综合类 | 259篇 |
农作物 | 50篇 |
水产渔业 | 157篇 |
畜牧兽医 | 766篇 |
园艺 | 45篇 |
植物保护 | 107篇 |
出版年
2023年 | 15篇 |
2022年 | 15篇 |
2021年 | 32篇 |
2020年 | 56篇 |
2019年 | 48篇 |
2018年 | 47篇 |
2017年 | 41篇 |
2016年 | 43篇 |
2015年 | 35篇 |
2014年 | 45篇 |
2013年 | 70篇 |
2012年 | 107篇 |
2011年 | 137篇 |
2010年 | 70篇 |
2009年 | 62篇 |
2008年 | 94篇 |
2007年 | 90篇 |
2006年 | 84篇 |
2005年 | 85篇 |
2004年 | 76篇 |
2003年 | 67篇 |
2002年 | 71篇 |
2001年 | 20篇 |
2000年 | 24篇 |
1999年 | 24篇 |
1998年 | 10篇 |
1997年 | 8篇 |
1996年 | 10篇 |
1995年 | 7篇 |
1994年 | 7篇 |
1993年 | 8篇 |
1991年 | 9篇 |
1990年 | 11篇 |
1989年 | 14篇 |
1988年 | 6篇 |
1987年 | 11篇 |
1986年 | 11篇 |
1985年 | 12篇 |
1984年 | 16篇 |
1983年 | 9篇 |
1980年 | 7篇 |
1978年 | 10篇 |
1977年 | 10篇 |
1976年 | 13篇 |
1975年 | 8篇 |
1974年 | 9篇 |
1972年 | 5篇 |
1971年 | 7篇 |
1970年 | 6篇 |
1969年 | 9篇 |
排序方式: 共有1726条查询结果,搜索用时 15 毫秒
211.
212.
蛋鸡生产者应基于沙门氏菌感染的风险和后果来评估预防其感染的成本和收益. 控制沙门氏菌感染如今正成为食用禽蛋生产的一个重要成本因素.欧盟出台的一系列旨在减少消费者感染沙门氏菌的法令,目的就是对整个生产链的操作流程进行评估.鸡蛋生产商应该利用现有的关于感染流行病学和控制措施方面的知识来制定禽舍建造、管理系统和禽群饲养(包括从饲料原料的质控到鸡蛋的产后处理、储存和运输)的决策.他们也应该评估在生产区域内由于沙门氏菌感染的风险和后果而采取的预防措施的成本和潜在效益. 相似文献
213.
In a number of countries, tuberculosis (due to infection with Mycobacterium bovis) is a significant health problem of captive deer. This paper describes outbreaks of bovine tuberculosis in sika deer (Cervus nippon) on two farms in Ireland and the methods used to control the disease. On Farm A, infection was first detected during 1993. The infection was eradicated using a programme of test and removal, in association with segregation of young animals. A second outbreak (also due to infection with M. bovis, but a different RFLP profile) was detected in 2002. In the latter outbreak, infection was particularly prevalent in two groups of young deer. M. bovis with the same RFLP profile was also isolated in a badger found dead on the farm. Control was achieved by test and removal in association with herd management changes. In Herd B, infection was first detected in 1995, and subsequently eradicated using test and removal alone. In Herd A, re-infection remains an ongoing risk. Control rather than eradication of infection may more realistic in the short-to medium-term. 相似文献
214.
Maluquer de Motes C Grassi J Simon S Herva ME Torres JM Pumarola M Girones R 《Veterinary microbiology》2008,131(1-2):205-211
Faeces from infected animals have been suggested as a potential source of contamination and transmission of prion diseases in the environment. This work describes the development of a procedure for the detection of PrP(res) in stools which is based on a detergent-based extraction and immunoprecipitation (IP). The procedure was evaluated by analyzing TSE-spiked sheep and mice faeces, and proved to be specific for PrP(res) with sensitivities of 5-10mug of infected brain tissue. In order to analyze the shedding of prions, we studied stools from orally inoculated mice over 4-days post-inoculation and also stools from terminally sick scrapie-infected mice. PrP(res) was only detected in stools shortly after the oral ingestion of TSE agents. The procedure described could be a useful tool for studying the excretion of prions and for evaluating potential environmental contamination by prions. 相似文献
215.
A male domestic shorthaired cat was presented for evaluation of stranguria and pollakiuria. A cryptococcal urinary tract infection (UTI) was diagnosed cytologically and via fungal culture. No evidence of systemic involvement was found. Chronic renal failure was a concurrent disease in this cat. Treatment consisted of oral fluconazole. Clinical signs resolved after 2 weeks of therapy, and fluconazole was discontinued after 6 months when negative urine culture results indicated resolution of the infection. This case demonstrates that correct identification of cryptococcal UTI allows for administration of therapy that can be associated with resolution of clinical signs. 相似文献
216.
Yueh SC Wang YH Lin KY Tseng CF Chu HP Chen KJ Wang SS Lai IH Mao SJ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(4):379-387
Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=43) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (<0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP_999165). The mean overall Hp level of the TLY minipigs (0.21 +/- 0.25 mg/ml; n=43) determined by enzyme-linked immunosorbent assay (ELISA) was markedly lower than that of domestic pigs (0.78 +/- 0.45 mg/ml; p<0.001), while 25% of the TLY minipigs had an Hp level that was extremely low (<0.05 mg/ml). In addition, the initial recovery rate (first 40 min) in the circulation of infused fluorescein isothiocyanate (FITC)-Hb was significantly higher in the TLY minipigs with extremely low Hp levels than those with high levels. This data suggests that the low concentration of Hp-Hb complex is responsible for the higher recovery rate of Hb in the circulation. TLY minipigs have been used as an experimental model for cardiovascular diseases; whether they can be used as a model for inflammatory diseases, with Hp as a marker, remains a topic of interest. However, since the Hp level varies significantly among individual TLY minipigs, it is necessary to prescreen the Hp levels of the animals to minimize variation in the experimental baseline. The present study may provide a reference value for future use of the TLY minipig as an animal model for inflammation-associated diseases. 相似文献
217.
Menzies-Gow NJ Bailey SR Berhane Y Brooks AC Elliott J 《American journal of veterinary research》2008,69(3):349-355
OBJECTIVE: To determine the effect of endotoxin (lipopolysaccharide [LPS]) on vasoactive mediator production by cultured equine digital vein endothelial cells (EDVECs). SAMPLE POPULATION: EDVECs obtained from forelimb digital veins of 7 healthy adult horses. PROCEDURES: EDVECs were incubated with or without LPS (1 microg/mL) for 0, 2, 4, 6, 22, and 24 hours. The EDVECs were incubated for 18 hours with LPS (10 pg/mL to 1 microg/mL) with or without ibuprofen, cycloheximide, or L-nitroarginine methyl ester. Medium concentrations of prostacyclin, cyclic guanosine monophosphate, endothelin-1, and thromboxane A(2) were determined. Changes in inducible nitric oxide synthase and cyclooxygenase-2 expression were determined. RESULTS: LPS stimulated mean 4.2- and 14.1-fold increases in EDVEC prostacyclin and cyclic guanosine monophosphate production, respectively, after 22 hours. These effects were LPS concentration-dependent (LPS concentrations that induced a response halfway between the maximum response and baseline of 1.50 and 1.22 ng/mL, respectively). The LPS-induced cyclic guanosine monophosphate production was significantly inhibited (to basal concentrations) by L-nitroarginine methyl ester, and prostacyclin production was inhibited by cycloheximide and ibuprofen. Production of thromboxane A(2) by EDVECs was not detected. Endothelin-1 accumulated in the medium, but LPS did not enhance its production. Inducible nitric oxide synthase expression in EDVECs was not detected with the available antibodies, whereas LPS stimulated cyclooxygenase-2 expression in a time- and concentration-dependent manner. CONCLUSIONS AND CLINICAL RELEVANCE: LPS stimulated vasoactive mediator production by equine endothelial cells, which may play a role in LPS-induced digital hypoperfusion. 相似文献
218.
Levine JM Levine GJ Boozer L Schatzberg SJ Platt SR Kent M Kerwin SC Fosgate GT 《Journal of the American Veterinary Medical Association》2008,232(3):411-417
OBJECTIVE: To determine complications and neurologic outcomes associated with dexamethasone administration to dogs with surgically treated thoracolumbar intervertebral disk herniation, compared with dogs not receiving dexamethasone. DESIGN: Retrospective case series. ANIMALS: 161 dogs with surgically confirmed thoracolumbar disk herniation. PROCEDURES: Medical records from 2 hospitals were used to identify dogs that had received dexamethasone < 48 hours prior to admission (dexamethasone group dogs), dogs that received glucocorticoids other than dexamethasone < 48 hours prior to admission (other-glucocorticoid group dogs), and dogs that received no glucocorticoids (nontreatment group dogs). Signalment, neurologic injury grade, laboratory data, and complications were extracted from medical records. RESULTS: Dexamethasone group dogs were 3.4 times as likely to have a complication, compared with other-glucocorticoid or nontreatment group dogs. Dexamethasone group dogs were 11.4 times as likely to have a urinary tract infection and 3.5 times as likely to have diarrhea, compared with other-glucocorticoid or nontreatment group dogs. No differences in neurologic function at discharge or recheck evaluation were detected among groups. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that treatment with dexamethasone before surgery is associated with more adverse effects, compared with treatment with glucocorticoids other than dexamethasone or no treatment with glucocorticoids, in dogs with thoracolumbar intervertebral disk herniation. In this study population, no difference in outcome was found among groups. These findings suggest that the value of dexamethasone administration before surgery in dogs with thoracolumbar disk herniation should be reconsidered. 相似文献
219.
Objective— To determine differences in bacterial strike-through for materials commonly used to cover the distal aspect of the pelvic limb during operative site preparation.
Study Design— Randomized block design; ex vivo model.
Animals— Canine cadaveric pelvic limbs (n=40).
Methods— Pelvic limbs (n=40) were randomly assigned to 4 treatment groups: Group 1=Vetrap™ +sterile Coban™ ; Group 2=latex glove+Vetrap™ +sterile Coban™ ; Group 3=latex glove+Vetrap™ +sterile Coban™ +sterile latex glove+sterile Coban™ ; and Group 4=latex glove+Vetrap™ +sterile disposable drape+sterile Coban™ . Limbs were contaminated with a standardized bacterial solution and routinely prepared using the assigned distal leg wrap. Bandages were fluid challenged with a saline (0.9% NaCl) solution-soaked laparotomy sponge for 30 seconds. The wrap surface was sampled for microbial culture before surgical preparation, immediately after, and 60 minutes after applying a sterile leg wrap.
Results— Bacterial growth occurred in all Group 1 cultures, 90% of Group 2 cultures, and none of the Group 3 and 4 cultures, 60 minutes after applying the sterile wrap.
Conclusion— A distal leg wrap of Vetrap™ +sterile Coban™ is not effective in preventing bacterial strike-through.
Clinical Relevance— If similar results occur in the live animal, then a sterile impermeable barrier must be incorporated into the distal leg wrap to prevent bacterial strike-through. 相似文献
Study Design— Randomized block design; ex vivo model.
Animals— Canine cadaveric pelvic limbs (n=40).
Methods— Pelvic limbs (n=40) were randomly assigned to 4 treatment groups: Group 1=Vetrap
Results— Bacterial growth occurred in all Group 1 cultures, 90% of Group 2 cultures, and none of the Group 3 and 4 cultures, 60 minutes after applying the sterile wrap.
Conclusion— A distal leg wrap of Vetrap
Clinical Relevance— If similar results occur in the live animal, then a sterile impermeable barrier must be incorporated into the distal leg wrap to prevent bacterial strike-through. 相似文献
220.
Artificial insemination with frozen-thawed spermatozoa is commonly used in cattle breeding. A simple and fast procedure is needed for routine evaluation of the acrosomal status of frozen-thawed bovine sperm. Therefore, the purpose of this study was to test two staining procedures used to determine the viability and integrity of acrosome of frozen-thawed bovine spermatozoa. Double staining and Hoechst/FITC-Pisum sativum agglutinin (FITC-PSA) labelling were tested for evaluating the viability and acrosome reaction induced by calcium ionophore of bull spermatozoa. In our experiments no significant differences were detected in the frequency of acrosome-reacted sperm either by double staining (37.98%) or by FITC-PSA labelling (39.33%). The viability of sperm stained by the double staining method was 67.17%, and a higher portion of viable sperm (82.67%) was observed by staining with the Hoechst procedure (P < 0.01). On the basis of the results obtained it is concluded that both methods can be used for detecting the acrosome reaction of frozen-thawed bovine spermatozoa. 相似文献