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961.
Plants of white clover (Trifolium repens L.) cultivar Crau, a self-fertile Crau genotype, and nine generations of inbred progeny were raised in sand culture in a
glasshouse experiment. Digital images of the root systems were made and root morphological characteristics were determined
on all the plants. Root architectural parameters were measured on the Crau parent and the S1, S4, S6, and S9 inbred lines. The clover roots became shorter and thicker with inbreeding but the number of root tips per plant was unchanged.
Root architecture (branching pattern) was largely unaffected by inbreeding. It is concluded that inbreeding white clover will
lead to shorter, thicker roots, and reduced nutrient uptake efficiency compared with the parent clover. The degree to which
these deleterious traits are overcome during the development of F1 hybrids needs to be determined. 相似文献
962.
Quantitative trait loci for phytate in rice grain and their relationship with grain micronutrient content 总被引:6,自引:0,他引:6
James C. R. Stangoulis Bao-Lam Huynh Ross M. Welch Eun-Young Choi Robin D. Graham 《Euphytica》2007,154(3):289-294
Phytate (inositol-hexa-phosphate) has an important role in plants but it also may have anti-nutritional properties in animals
and humans. While there is debate within the plant breeding and nutrition communities regarding an optimum level in grain,
there appears to be little information at the molecular level for the genetics of this trait, and its association with important
trace elements, in particular, Fe and Zn. In this preliminary study, quantitative trait loci (QTL) for grain phytates, Zn
and Fe in glasshouse-grown rice lines from an IR64 × Azucena doubled haploid population were identified. Correlations between
phytate and essential nutrients were also studied. Transgressive segregation was found for most traits. Phytate and total
P concentrations had one QTL in common located on chromosome five with the (high concentration) allele contributed from Azucena.
There were significant positive correlations between phytate and inorganic phosphorus (P), total P, Fe, Zn, Cu and Mn concentrations
for both grain concentration and content. However, the QTLs of phytate were not located on the same chromosomal regions as
those found for Fe, Zn and Mn, suggesting that they were genetically different and thus using molecular markers in breeding
and selection would modify the phytate level without affecting grain micronutrient density. 相似文献
963.
Ram C. Sharma G. Ortiz-Ferrara J. Crossa M. R. Bhatta M. A. Sufian J. Shoran A. K. Joshi R. Chand Gyanendra Singh R. Ortiz 《Euphytica》2007,157(3):457-464
Improving the level and stability of grain yield is the primary objective of wheat breeding programs in the Eastern Gangetic
Plains (EGP) of South Asia. A regional wheat trial, the Eastern Gangetic Plains Yield Trial (EGPYT), was initiated by CIMMYT
in collaboration with national wheat research programs in Bangladesh, Nepal, and India in 1999–2000 to identify wheat genotypes
with high and stable grain yield, disease resistance, and superior agronomic traits for the EGP region. A set of 21 wheat
experimental genotypes selected from a regional wheat screening nursery in South Asia, three improved widely grown cultivars
(Kanchan, PBW343 and Bhrikuti), and one long-term cultivar (Sonalika) were tested at 9–11 sites in six wheat growing seasons
(2000–2005) in the EGP. The 21 experimental genotypes were different in each year, whereas the four check cultivars were common.
In each year, one or more of the experimental genotypes showed high and stable grain yield and acceptable maturity, plant
height, and disease resistance compared to the check cultivars. Three improved cultivars have already been commercially released
in the region through EGPYT and many germplasm lines have been used in the breeding programs as parents. Identification of
wheat genotypes with high-grain yield in individual sites and high and stable yield across the EGP region underlines their
value for regional wheat breeding programs attempting to improve grain yield and agronomic performance. 相似文献
964.
Tartary buckwheat (Fagopyrum tataricum) is a highly nutritious crop that is widely grown in Asia, but the flour contains a large portion of the hull if it is ground
with industrial processing since the hull is very hard to remove as it adheres to the testa layer of the groat. Rice-Tartary,
a particular type of Tartary buckwheat, has seeds with a loose hull and the presence of splits on the sides of the seeds that
make dehulling easily. The present study reports on the first attempt of crop improvement in Tartary buckwheat breeding through
hybridization. Hybrids were obtained by hybridization of crosses between Tartary buckwheat and Rice-Tartary. Additional crosses
were made among selected progenies of these crosses. Based on progeny analysis of the F1, F2, and F3, the character of Rice-Tartary, as exhibited as the presence of splits on the sides of the seeds, is controlled by one gene
which is homozygous recessive. A Tartary buckwheat breeding program has been conducted for 6 years based on these crosses.
Advanced lines with easy dehull and yield potential are at the stage of ready for yield trials. Production of easy dehulling
Tartary buckwheat in the future could boost Canada’s domestic and international markets. 相似文献
965.
The height-reducing gene Rht8 was introduced into Italian wheats by breeder Nazareno Strampelli from the Japanese landrace Akakomugi, and has been widely
used in wheats adapted to southern and eastern European conditions. Following identification of a close linkage to Rht8, microsatellite marker Gwm261 has been used extensively to screen large numbers of diverse international germplasm. A 192bp allele at this locus has been
taken as “diagnostic” for Rht8 and used to infer the international distribution of Rht8. In this paper, we report several instances of cultivars and mapping populations that vary for the presence of the 192bp
allele at the Xgwm261 locus (Xgwm261
192
), but with no associated reduction in height, suggesting a lack of association with Rht8. For instance, in the population derived from a cross between Sunco (Rht-B1b, Xgwm261
165
) and Tasman (Rht-D1b, Xgwm261
192
), there were significant height differences associated with the segregation of Rht-B1b and Rht-D1b, but no height differences between Xgwm261 genotypes. Similar results were obtained in a population derived from the cross between Molineux (Rht-B1b, Xgwm261
192
) and Trident (Rht-D1b Xgwm261
208
). In contrast, the cross between Trident and Chuanmai 18 (Xgwm261
192
) gave significant height effects at both the Rht-D1 and Xgwm261 loci, with no epistatic interaction between loci. Chuanmai 18 is closely related to the Strampelli wheat Mara (ancestrally
derived from Akakomugi) and is therefore likely to carry Rht8. The old Japanese cultivar Norin 10, used by Norman Borlaug to introduce Rht-B1b and Rht-D1b into Mexican wheats, also has a 192bp allele at the Xgwm261 locus, and the sequence of the amplified product is identical to that of Akakomugi. We suggest that the widespread use of
Norin 10-derived germplasm during and after the Green Revolution introduced a second haplotype into international germplasm,
in which Xgwm261
192
has no association with Rht8. Therefore, the presence of Xgwm261
192
is only indicative of Rht8 in wheat cultivars that have inherited this allele from Akakomugi or a Strampelli wheat ancestor. 相似文献
966.
Two wide hybrids, Diplotaxis erucoides (2n = 14) × Brassica rapa (2n = 20) and B. maurorum (2n = 20) × B. rapa, were developed using the sequential ovary–ovule culture. Reciprocal crosses failed, possibly as a consequence of strong
unilateral incompatibility. The F
1 hybrids in each combination were completely male sterile and morphologically intermediate to the respective parents. DNA
marker polymorphism and chromosome counts confirmed their hybrid nature. High frequency of bivalents in the F
1 and the presence of trivalents/quadrivalents in the derived amphiploids suggested genomic duplications and homoeology of the
parental genomes. Up to three homoeologous pairs between the D. erucoides (DeDe) and B. rapa (AA) genomes, and one between B. maurorum (BmBm) and B. rapa genomes were observed. Successful synthesis of the F
1 hybrids and amphiploids of B. rapa with D. erucoides and B. maurorum, and allosyndetic chromosome pairing are expected to permit introgressions of desirable loci into the cultivated Brassica
germplasm, especially for resistance to Alternaria
brassicae and Albugo candida. 相似文献
967.
E. O. Rueda-Puente J. L. García-Hernández P. Preciado-Rangel B. Murillo-Amador M. A. Tarazón-Herrera A. Flores-Hernández J. Holguin-Peña A. N. Aybar J. M. Barrón Hoyos D. Weimers O. Mwandemele G. Kaaya J. L. Mayoral E. Troyo-Diéguez 《Journal of Agronomy and Crop Science》2007,193(3):167-176
Salinity is a major stress condition. Salicornia bigelovii is a valuable edible halophyte, considered to be a promising resource for cultivation in arid coastal zones. Its productivity depends on the supplementary provision of nitrogen, for which an option is chemical fertilization. Nevertheless, indiscriminate use of chemical fertilizers contributes to the problem of increased salinity. The inoculation of plant growth‐promoting bacteria (PGPB) represents an alternative. Seed ecotypes from four coastal areas [Santa Rosa Chica, Santa Rosa Grande, Santa Cruz and Cerro Prieto (CP), Sonora, México] were collected, in order to inoculate them with two species of PGPB (Azospirillum halopraeferens and Klebsiella pneumoniae). Two germination tests were carried out to study the effect of salinity, temperature regime (night/day) and inoculation with PGPB on germination (percentage and rate), plant height, root length and biomass produced (fresh and dry matter). In the first test, all four ecotypes were considered, whereas in the second test only the CP ecotype was involved because it was found to be the outstanding ecotype in the previous test. Results showed inhibition of germination when salinity was higher in all ecotypes except CP. The CP ecotype showed a decrease of seed germination with an increase in NaCl concentrations at all temperatures tested. However, when it was inoculated with both PGPB, the germination percentage was influenced. 相似文献
968.
Promoter anchored amplified polymorphism based on random amplified polymorphic DNA (PAAP-RAPD) in cotton 总被引:1,自引:0,他引:1
Non-coding sequences account for a majority of the higher plant genome, some of which have important effects in gene regulation
and plant development. In an effort to develop molecular marker systems to search for polymorphisms associated with high fiber
yield and quality in cotton, we have developed a methodology that could specifically target the regulatory regions of the
cotton genome. In this study we designed 10-nucleotide degenerate promoter primers based on conserved core promoter sequences
and tested their applicability in PCR amplifications in combination with 10-mer random amplified polymorphic DNA (RAPD) primers.
The amplified markers are called promoter anchored amplified polymorphism based on RAPD (PAAP-RAPD). Forty cotton genotypes
with diverse genetic and geographical backgrounds were used to test the PAAP-RAPD system using polyacrylamide gel electrophoresis.
Based on PAAP-RAPD markers amplified from 12 primer combinations, the 40 genotypes were classified into five distinctive groups:
two Upland cotton (Gossypium hirsutum) groups from China, another two Upland cotton groups from the USA, and one group from American Pima cotton (G. barbadense). The groupings are in general consistent with their genetic and geographical origins. Thirty-six PAAP-RAPD and RAPD fragments
were cloned and four of them were further subjected to sequence analysis. Signal scanning using software PLACE confirmed that
they contained an array of cis-regulatory sequences in addition to the core promoter sequences. The results demonstrate the
potential application of PAAP-RAPD as a new marker system specifically targeting regulatory regions of the plant genome. 相似文献
969.
Mun Sup Yoon Jeongran Lee Chang Yung Kim Jung Hoon Kang Eun Gi Cho Hyung Jin Baek 《Euphytica》2009,165(1):69-77
Approximately 7,000 accessions of Korean soybean (Glycine max (L.) Merrill) landraces, largely composed of three collections, the Korea Atomic Energy Research Institute’s soybean (KAS),
the Korean Crop Experiment Station’s soybean (KLS) and the Korean Agricultural Development and Technology Center’s soybean
(KADTC) collections, have been conserved at the Rural Development Administration (RDA) genebank in Korea. The accessions within
collections were classified based on their traditional uses such as sauce soybean (SA), sprouted soybean (SP), soybean for
cooking with rice (SCR), and OTHERS. A total of 2,758 accessions of Korean soybean landraces were used to profile and to evaluate
genetic structure using six SSR loci. A total of 110 alleles were revealed by at the six SSR loci. The number of alleles per
SSR locus ranged from 9 to 39 in Satt187 and Satt_074, respectively. The number of alleles ranged from 87 in the KADTC collection
to 96 in the KLS collection, and from 63 in the SCR group to 95 in the SP group. Nei’s average genetic diversity ranged from
0.68 to 0.70 across three collections, and 0.64 to 0.69 across the usage groups. The average between-group differentiation
(G
st) was 0.9 among collections, and 4.1 among the usage groups. The similar average diversity among three collections implies
that the genetic background of the three collections was quite similar or that there were a large number of duplicate accessions
in three collections. The selection from the four groups classified based upon usage may be a useful way to select accessions
for developing a Korean soybean landrace core collection at the RDA genebank. DNA profile information of accessions will provide
indications of redundancies or omissions and aid in managing the soybean collection held at the RDA genebank. The information
on diversity analysis could help to enlarge the genetic diversity of materials in breeding programs and could be used to develop
a core collection. 相似文献
970.
Helleborus species are members of the family of the Ranunculaceae. These popular perennials are all diploids (2n = 2x = 32). This study investigates polyploidy induction by different antimitotic agents. Colchicine, oryzalin and trifluralin
were tested in vitro on shoots of Helleborus niger, H. orientalis and H. × nigercors. Furthermore the effect of the antimitotic agents on the viability and the multiplication rate of cultured plantlets were
analyzed. Flow cytometry demonstrated that polyploidisation was genotype dependent: using H. niger, tetraploids were obtained using either oryzalin (3 μM) or trifluralin (3 or 10 μM), whereas for H. × nigercors only trifluralin (3 or 10 μM) induced polyploidisation. For H. orientalis neither treatment was effective to produce tetraploids or mixoploids. For these three species, colchicine (100 μM) was ineffective.
The polyploidisation events in H. niger and H. × nigercors were confirmed by chromosome counts of mounted nuclei derived from root tips (2n = 4x = 64). 相似文献