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排序方式: 共有137条查询结果,搜索用时 15 毫秒
101.
Furukawa S Nagashima Y Hoshi K Hirao H Tanaka R Maruo K Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(1):41-44
In veterinary medicine, dopamine is currently being administered clinically by infusion for treatment of kidney disorders at low doses (< or = 3 microg/kg/min) and for assessment of hemodynamics at high doses (> or = 5 microg/kg/min). However, since high doses of dopamine cause peripheral vasoconstriction due to its effect on alpha adrenoceptors, high doses have no longer been recommended. The present study was conducted to explore possible regimens for the use of dopamine infusion in dogs. The regional (renal and cardiac) blood flow for 60 min was measured by using colored microspheres at three doses (3, 10 and 20 microg/kg/min) of dopamine infusion in healthy anesthetized mongrel dogs. The effects on kidney and peripheral hemodynamics at each dose and the resultant cardiac output, mean arterial blood pressure and total peripheral resistance were determined. Renal blood flow increased markedly at 3 microg/kg/min dopamine. Improvement in hemodynamics indicated by marked increase in cardiac blood flow, cardiac output and mean arterial blood pressure and decreased total peripheral resistance was observed at higher doses (10 and 20 microg/kg/min). At 10 microg/kg/min, in addition to the satisfactory increase in cardiac blood flow, there was also a stable satisfactory increase in renal blood flow. However, at 20 microg/kg/min, increased myocardial oxygen consumption (manifested by marked increased in cardiac output), arrythmia and irregular increase in renal blood flow were detected. This study suggests that the clinical use of dopamine infusion in dogs could be safely expanded to moderately higher doses. 相似文献
102.
Molnar P Burchfiel BC Ziyun Z K'uangyi L Shuji W Minmin H 《Science (New York, N.Y.)》1987,235(4786):299-305
A reconnaissance expedition across the northern margin of the Tibetan plateau revealed evidence of a late Cenozoic northward progression of the locus of crustal shortening and, therefore, of a northward growth of the area encompassed by the plateau. Active reverse faults crop out at the foot of the Altyn Tagh, on the northern edge of the plateau, and at the bases of several ranges within the Altyn Tagh and Kunlun, where the elevations of the neighboring basins are less than 4000 meters. Farther south, where elevations are higher, there was no evidence of recent faulting, but late Cenozoic rock in the Ayak Kum K?l basin has been strongly folded. South of this basin, Ulugh Muztagh, apparently the highest mountain in the eastern Kunlun, is underlain by late Miocene, tourmaline-bearing and two-mica granite. These rocks suggest that thickening of continental crust had begun in this area by late Miocene time. Overlying quartz-sanidine welded tuffs of Pliocene age imply that uplift and erosion occurred between Miocene and Pliocene time, but with little subsequent erosion. In addition, we found an east-west trending belt of mafic and ultramafic rock that probably marks a suture of a crustal fragment with southern Asia in Triassic or more recent time. 相似文献
103.
Structural differences between myofibrillar protein,paratropomyosin, and tropomyosin as revealed by high‐performance liquid chromatography
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Yuriko Nishio Yuichi Ushimura Shuji Ueda Naoyuki Maeda Akihito Hattori Minoru Yamanoue 《Animal Science Journal》2018,89(8):1161-1168
Paratropomyosin (PTM) composes myofibril functions to weaken the rigor linkages formed between actin and myosin during postmortem aging of muscles. PTM has the similar physico‐chemical properties as tropomyosin (TM) that is a regulatory protein of myofibrils. So far, it is unclear whether PTM is definitely different from TM, because the primary structure of PTM has not been determined yet. The aim of this study was to clarify structural difference of PTM from TM. PTM was prepared by column chromatography immediately after slaughter from broiler breast muscle, and purified by high‐performance liquid chromatography (HPLC). Purified PTM was successfully separated from TM, and the recovered PTM molecule was reduced with dithiothreitol to separate again by HPLC. Two subunits were obtained and peptides from each digested subunit by V8 protease were recovered by HPLC, and then amino acid sequences of the peptides were analyzed by protein sequencing. As a result, some amino acid residues were replaced from that of TMα1 isoform which is the major isoform of TM, and also was different between the two subunits. Therefore, it is concluded that PTM clearly differs from TM and it is suggested that functional difference in PTM from TM is attributed to amino acid replacements in subunits composing PTM. 相似文献
104.
105.
Mitani S Ouchi A Watanabe E Kanesaki Y Nagaoka S Mukai K 《Journal of agricultural and food chemistry》2008,56(12):4406-4417
Kinetic study of the aroxyl radical-scavenging action of catechins (epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and epigallocatechin gallate (EGCG)) and related compounds (methyl gallate (MG), 4-methylcatechol (MC), and 5-methoxyresorcinol (MR)) has been performed. The second-order rate constant ( k s) for the reaction of these antioxidants with aroxyl radical has been measured in ethanol and aqueous Triton X-100 micellar solution (5.0 wt %). The k s values decreased in the order of EGCG > EGC > MC > ECG > EC > MG > MR in ethanol, indicating that the reactivity of the OH groups in catechins decreased in the order of pyrogallol B-ring > catechol B-ring > gallate G-ring > resorcinol A-ring. The structure-activity relationship in the free radical-scavenging reaction by catechins has been clarified by the detailed analyses of the pH dependence of k s values. From the results, the p K a values have been determined for catechins. The monoanion form at catechol B- and resorcinol A-rings and dianion form at pyrogallol B- and gallate G-rings show the highest activity for free radical scavenging. It was found that the free radical-scavenging activities of catechins are 3.2-13 times larger than that of vitamin C at pH 7.0. 相似文献
106.
为研究河鲀鱼精蛋白的制备工艺,以菊黄东方鲀(Takifugu Flavidus)精巢为原料,以鱼精蛋白提取率为指标,通过单因素试验研究NaCl溶液的pH、硫酸浓度、硫酸用量、提取温度、提取时间以及乙醇用量等单因素对鱼精蛋白提取率的影响,在此基础上,采用响应面法优化鱼精蛋白的提取工艺条件。结果显示,采用响应面法优化获得河鲀鱼精蛋白的最佳提取工艺条件为:NaCl溶液pH 8.5,硫酸浓度0.6 mol/L,硫酸用量4.0倍沉淀体积,提取温度45℃,提取时间2.4 h,乙醇用量4.0倍沉淀体积,此时鱼精蛋白提取率达到19.07%。研究表明,响应面法对河鲀鱼精蛋白提取条件的优化方案合理可行,可为河鲀精巢的开发利用提供参考依据。 相似文献
107.
Yongchang Su Shicheng Chen Shuilin Cai Shuji Liu Nan Pan Jie Su Kun Qiao Min Xu Bei Chen Suping Yang Zhiyu Liu 《Marine drugs》2021,19(12)
Alcalase, neutral protease, and pepsin were used to hydrolyze the skin of Takifugu flavidus. The T. flavidus hydrolysates (TFHs) with the maximum degree of hydrolysis (DH) and angiotensin-I-converting enzyme (ACE)-inhibitory activity were selected and then ultra-filtered to obtain fractions with components of different molecular weights (MWs) (<1, 1–3, 3–10, 10–50, and >50 kDa). The components with MWs < 1 kDa showed the strongest ACE-inhibitory activity with a half-maximal inhibitory concentration (IC50) of 0.58 mg/mL. Purification and identification using semi-preparative liquid chromatography, Sephadex G-15 gel chromatography, RP-HPLC, and LC–MS/MS yielded one new potential ACE-inhibitory peptide, PPLLFAAL (non-competitive suppression mode; IC50 of 28 μmmol·L−1). Molecular docking and molecular dynamics simulations indicated that the peptides should bind well to ACE and interact with amino acid residues and the zinc ion at the ACE active site. Furthermore, a short-term assay of antihypertensive activity in spontaneously hypertensive rats (SHRs) revealed that PPLLFAAL could significantly decrease the systolic blood pressure (SBP) and diastolic blood pressure (DBP) of SHRs after intravenous administration. These results suggested that PPLLFAAL may have potential applications in functional foods or pharmaceuticals as an antihypertensive agent. 相似文献
108.
Steven D. Tanksley Dario Bernachi Teresa Beck-Bunn Davy Emmatty Yuval Eshed Shuji Inai Joachin Lopez Vincent Petiard Haruki Sayama John Uhlig Daniel Zamir 《Euphytica》1998,99(2):77-83
A pair of processing tomato (Lycopersicon esculentum Mill.) lines, nearly isogenic for the Tm-2a gene for resistance to tobacco mosaic virus, were grown in replicated trials under commercial production conditions in five
locations worldwide. The lines were evaluated for 17 processing traits including fruit yield, size, soluble solids concentration,
color, firmness, and viscosity. Eight of those traits differed significantly among the nearly-isogenic lines (NILs). Most
notably, the NIL heterozygous for Tm-2a yielded, on average, 16% more than the NIL homozygous for the susceptible allele and 33% more than the NIL homozygous for
Tm-2a. Viscosity was lower in the heterozygous NIL and the homozygous and heterozygous resistant NILs had softer fruit with larger
stem scars compared to the homozygous susceptible NIL. These results indicate the presence of the Tm-2a locus affects many traits of importance for processing tomatoes and may be used, in the heterozygous state, to significantly
increase yield. Whether the observed effects are due to the Tm-2a gene itself or genes associated via linkage drag could not be determined.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
109.
110.
Kiyoshi AKIYAMA Jun KOBAYASHI Yoshimasa SATO Ryuichi SATA Megumi OHASHI Emi SASAKI Yorimasa ODA Yoshio OGAWA Shuji UEDA Hisashi NABENISHI Satoko MATOBA 《Animal Science Journal》2010,81(4):461-466
The objective of this study was to develop an in‐straw dilution method suitable for direct transfer of vitrified bovine sexed embryos. Embryo sexing was performed by molecular diagnosis. Several sexed and vitrified‐warmed embryos were transferred after evaluation of morphologically embryonic survival at warming and in‐straw dilution (Evaluation group). The other embryos were immediately directly transferred to recipients without first being expelled from the straws after in‐straw dilution (Non‐evaluation group). The pregnancy rates of vitrified sexed embryos were 38.7% and 34.8% in the Evaluation group and Non‐evaluation group, respectively, which were not significantly different. The viability of lower quality embryos before vitrification tended to be lower (P = 0.087) than that of the higher quality embryos regardless of evaluating embryos after warming and in‐straw dilution. The abortion rates were similar, and there was no difference between the two groups (13.9% and 12.5%, respectively). These results demonstrate that vitrified bovine sexed embryos can be vitrified and diluted by the in‐straw method and that the vitrified and warmed sexed embryos can develop to term. 相似文献