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Yufei Guo Chanjuan Shen Anchun Cheng Mingshu Wang Na Zhang Shun Chen Yi Zhou 《Veterinary microbiology》2009,138(3-4):258-265
Anatid herpesvirus 1 (AHV-1) CH virulent strain was first isolated from an infected duck and it was found that this virus strain could induce cytopathic effect (CPE) in duck embryo fibroblast (DEF). Following AHV-1 infection, DEF showed morphological changes such as cell rounding, improved refractivity and detachment from the culture surface. However, its pathological characteristics were not adequately known. Related studies were performed and the results showed that syncytium formation could be observed as the other type of CPE in AHV-1 infection. Hematoxylin-eosin staining and 4’, 6-diamidino-2-phenylindole (DAPI) staining of infected DEF were each used to visualize the shape and distribution of chromatin within nuclei and nuclear fragmentation was observed. Chromatin condensation and margination, as well as formation of apoptotic bodies were observed by transmission electron microscopy (TEM). DNA ladder formation was detected in AHV-1 infected cells and apoptosis of the infected DEF was also detected by flow cytometry analysis of Annexin V-FITC/PI staining method. Therefore, it was suggested that AHV-1 virulent strain can induce syncytium and apoptosis in DEF. Syncytium formation and apoptosis observed in this study may contribute to the elucidation of AHV-1 pathogenesis. 相似文献
23.
从绿色管理的概念出发,在研究园林绿化产业巨大的环境保护与生态效益的基础之上,探讨了将绿色管理思想运用到园林绿化产业中,并从园林绿化设计、施工、养护、废弃物处理4个环节具体分析了绿色管理在园林绿化产业的运用. 相似文献
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激素与营养液配施对平菇产量及品质的影响 总被引:10,自引:0,他引:10
植物激素与营养液配合施用可促进平菇菌丝生长和子实体发育,提高产量,改善商品性状,但却不能增加干物质的积累。对营养成分含量的影响随激素与营养液配方的不同而异。 相似文献
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The performance of insect-resistant transgenic poplar trees (Populusnigra) expressing a Cry1Ac gene from Bacillus thuringiensis subsp. Kurstaki HD-1 against poplar defoliators was evaluated in the field at the Manas Forest Station in Xinjiang Uygur
Autonomous Region during1994–1997. The results showed that the average percentage of highly damaged leaves on the transgenic
trees was 10% while that on the control trees in nearby plantations reached 80–90%. The average number of pupae per m2 of soil at 20cm depth in transgenic poplar plantation was 18 which was only 20% of that found in the non-transgenic control
field. The number of pupae and the leaf-damage on transgenic trees described above are all far below the threshold set for
chemical protection measures. The non-transformed poplar trees grown in the same plantation with the transgenic trees were
also protected indicating that cross protection occurred between these two kinds of trees. Insect-resistant transgenic poplar
trees have a potential application value in afforestation.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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根据关中灌区自然经济条件,提出了一种新的机械化模式。经过9年实践表明,采用该模式,无论丰年、平年、灾年均可大幅度增产,能提高抵抗自然灾害的能力,使丰、灾年间产量波幅减小,更趋稳定。 相似文献
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Liu Zhongxuan Han Junling Liu Zhihui Zhang Xingyao Chen Jie Dong Airong Liu Xuefeng 《植物病害和植物保护杂志》2019,126(5):485-488
Journal of Plant Diseases and Protection - In May 2018, a bacterial tumor disease was observed on Populus koreana in Shuangyang county, Changchun city, Jilin Province. Based on physiological... 相似文献