Characterization of tilapia (Oreochromis niloticus) viperin expression,and inhibition of bacterial growth and modulation of immune-related gene expression by electrotransfer of viperin DNA into zebrafish muscle

Viperin is an anti-viral protein, induced by viral infection. In this study, we examined whether over-expression of viperin in fish muscle could inhibit bacterial growth. We first obtained the cDNA sequence of tilapia viperin, through RT-PCR-mediated cloning and sequencing. The cDNA sequence was similar to those of several fish viperins in GenBank, and it was predicted to encode the conserved domain of radical S-adenosylmethionine superfamily proteins. Phylogenetic analysis revealed that tilapia viperin was most closely related to viperin of Sciaenops ocellatus, Coreoperca kawamebari, and C. whiteheadi. Expression of tilapia viperin was significantly up-regulated in the kidney, liver, spleen, and gills upon challenge with lipopolysaccharide (LPS) and poly(I:C) in a time- and dose-dependent manner. Injection of Vibrio vulnificus (204) and Streptococcus agalactiae (SA47) bacteria into tilapia resulted in significant induction of viperin expression in the whole body, kidney, liver, and spleen. Electrotransfer of a viperin-expressing plasmid into zebrafish muscles decreased bacterial numbers and altered expression of immune-related genes. These data indicate that such altered expression may account for the improvement in bacterial clearance following electroporation of viperin, suggesting that fish viperin has antiviral and antibacterial activities.     

复合加固梁的裂缝开展及破坏现象分析

本文通过试验测试介绍了混凝土复合加固梁在荷载作用下的裂缝开展情况及破坏现象,分析其发生的原因及机理.为预应力碳纤维布与钢板复合加固混凝土受弯构件的应用提供参考依据.     

Design, synthesis, and fungicidal activity of macrolactones and macrolactams with a sulfonamide side chain

Four series of novel macrolactones and macrolactams12-alkylsulfonamido-1,15-pentadecanlactones ( 5), 12-alkylsulfonamido-15-methyl-1,15-pentadecanlactones ( 6), 12-alkylsulfonamido-1,15-pentadecanlactams ( 7), and N-(alkylsulfonamidoethyl)-1,12-dodecanlactams ( 8)were designed and synthesized from readily available 2-nitrocyclododecanone or cyclododecanone. Their structures were confirmed by (1)H NMR, IR, and elemental analysis. The bioassay showed that these compounds displayed fair to excellent fungicidal activity against Rhizoctonia solani Kuhn and have a gradual increase of fungicidal activity in the order of 6, 7, 8, and 5. Among them, compounds 5a, 5b, and 5c displayed excellent fungicidal activity against R. solani comparable with the commercial fungicide carbendazim. Above results illustrated that the rule on the relationship between the activity and hydrogen-bonding, namely the macrocyclic compounds with a hydrogen-bonding acceptor and a hydrogen-bonding donor on the ring and having a three methylenes distance between two polarizable groups have the best fungicidal activity against R. solani, has a general suitability to the macrocyclic compounds, and pesticide molecules may combine with a target enzyme by hydrogen-bonding. The facts, which compound 6 has a much lower fungicidal activity against R. solani than compound 5 but their difference in chemical structure is only that there is a methyl group on the C15 for compound 6 and none but hydrogen atom on the C15 for compound 5, indicated that a methyl group plays an inhibitory role to the fungicidal activity. It suggests that the existence of a methyl group with a great volume between two polarizable groups would interfere in the interaction of pesticide molecules and the target enzyme.     

蔬菜温棚土壤钾素形态及空间变异的研究

2003年在宁夏银川市选择具有代表性的灌淤土为供试土壤,对不同种植年限蔬菜温棚土壤钾素状况进行了调查研究。结果表明,蔬菜温棚0～30cm土层土壤全钾、缓效钾含量与农田和露地菜田变化不大,但速效钾含量变化较大,其中新建温棚较农田高2.8倍,3a温棚较农田高2.9倍,8a、15a温棚分别较农田高1.4、1.7倍。在蔬菜温棚中随着土层深度的增加,蔬菜温棚土壤全钾、速效钾、缓效钾含量均有不同程度减少,其中全钾、缓效钾减幅不大,速效钾减幅较大。     

小麦群体图像特征识别方法的研究 ——小麦群体总茎数的估测

作物群体特征一肌指群体内个体的数量、种类、生育状况及其空间排列、分布和个体间的关联程度等.群体内光照、温度、湿度、风和二氧化碳浓度等要素的分布均与作物群体性状紧密相连,因此,对群体结构特征的考察一直受到关注,也研制了许多考察的方法[1~3],这些方法都曾为农业科研和生产做出过贡献.     

Synthesis and fungicidal activity of macrolactams and macrolactones with an oxime ether side chain

Three series of novel macrolactams and macrolactones--12-alkoxyimino-tetradecanlactam, 12-alkoxyiminopentadecanlactam, and 12-alkoxyiminodecanlactone derivatives (7A, 7B, and 7C)--were synthesized from corresponding 12-oxomacrolactams and 12-oxomacrolactone. Their structures were confirmed by 1H NMR and elemental analysis. The Z and E isomers of 7A and 7B were separated, and their configurations were determined by 1H NMR. These compounds showed fair to excellent fungicidal activities against Rhizoctonia solani Kühn. It is interesting that the Z and E isomers of most of the compounds have quite different fungicidal activities. The fact that the compounds have a gradual increase of fungicidal activity in the order of 7A, 7C, and 7B indicated that the macrocyclic derivatives with a hydrogen-bonding acceptor (=N-O-) and a hydrogen-bonding donor (-CONH-) on the ring, and a three methylenes distance (CH2CH2CH2) between these two functional groups, exhibited the best fungicidal activity. The bioassay also showed that 7B not only has good fungicidal activity but also may have a broad spectrum of fungicidal activities.     

金沙江干热河谷区甜椒露地高产栽培技术

云南是锥形甜椒红椒上市的主产区,主要供应国内12 月至翌年5 月的红色甜椒市场,同时部分红椒还可以出口。金沙江干热河谷区冬早甜椒高产种植技术的推广应用,对改善金沙江流域冬春蔬菜品种结构,促进农民增收具有重要意义。     

Alkaloids and sesquiterpenes from the South China Sea gorgonian Echinogorgia pseudossapo

Five zoanthoxanthin alkaloids (1-5) and four sesquiterpenes (6-9) were isolated from the South China Sea gorgonian Echinogorgia pseudossapo. Their structures were determined on the bases of extensive spectroscopic analyses, including 1D and 2D NMR data. Among them, pseudozoanthoxanthins III and IV (1-2), 8-hydroxy-6β-methoxy-14- oxooplop-6,12-olide (6) and 3β-methoxyguaian-10(14)-en-2β-ol (7) were new, 1 and 3 showed mild anti-HSV-1 activity, and 7 showed significant antilarval activity towards Balanus amphitrite larvae.     

玉米不同生育阶段养分效应评价方法研究

了解作物养分效应机制是提高作物养分利用效率的重要基础。本文以玉米品种"农华101"为试验材料, 进行不同播期、密度、肥料试验, 记录了生育时期的日均温, 并对玉米不同生育时期叶片及其他器官中氮、磷、钾元素含量进行了测定, 然后基于改进后的BLUP方法, 在考虑植株干物质日均积累量与自身效应、环境效应关系的基础上, 引入群体效应和温度效应, 探讨并建立了玉米不同生育阶段养分效应评价方法。在此基础上, 进一步对养分效应评价效果进行分析。结果表明: 干物质日均积累量理论值与实际值的相关性r值为0.72~0.99; 各生育阶段配对t检验的α值均大于0.05, 各生育阶段的干物质日均积累量的理论值和实测值差异均不显著(P>0.05)。研究结果表明, 用建立的玉米不同生育阶段的养分效应评价方法得到的各养分特征效应值存在较大差异, 其中: 三叶期到拔节期各养分效应值为0~0.58, 拔节期到大口期为 0.43~2.57, 大口期到吐丝期为 0.45~4.58, 吐丝期到成熟期为 0.86~4.71。综上, 利用改进后的BLUP法提出的养分效应评价方法对玉米各阶段养分效应的评价结果具有一定的指导意义, 该方法可作为玉米养分效应评价的参考手段。