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101.
In-Sun Park Momoko Koiso Satomi Morimoto Takafumi Kubo Hyun-O Jin Ryo Funada 《Journal of Wood Science》2012,58(1):64-68
We attempted to develop a method for the regeneration of plantlets from mature seeds of medically important Magnolia obovata via the induction of somatic embryogenesis in vitro. We initially cultured halves of mature seeds on either Murashige and
Skoog (MS) medium or B5 medium that contained 0, 1, 5 or 10 μM gibberellic acid (GA3) for 1 month and then transferred the half-seeds to half-strength MS basal medium or B5 basal medium for further culture in the absence of GA3. Proembryogenic masses (PEMs) were observed 1 month after the transfer of the halved mature seeds to the medium without GA3. The frequency of formation of PEMs was higher (28%) after initial culture in MS basal medium plus 1 μM GA3 than in other tested media (0 or 4%). Somatic embryos that had been developed from PEMs were cultured on half-strength MS
basal medium or B5 basal medium for completion of maturation and then transferred to fresh aliquots of the same medium for initiation of germination.
The frequency of germination, with the formation of normal primary leaves and roots, was above 80%. We transferred the somatic
embryo-derived plantlets to soil for acclimatization and the plantlets continued to thrive. 相似文献
102.
Mohammed Ahmed UMAR Sho FUKUI Kodai KAWASE Takaharu ITAMI Kazuto YAMASHITA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):281-288
Cardiovascular effects of total intravenous anesthesia using
ketamine-medetomidine-propofol drug combination (KMP-TIVA) were determined in 5
Thoroughbred horses undergoing surgery. The horses were anesthetized with intravenous
administration (IV) of ketamine (2.5 mg/kg) and midazolam (0.04 mg/kg) following
premedication with medetomidne (5 µg/kg, IV) and artificially ventilated.
Surgical anesthesia was maintained by controlling propofol infusion rate (initially 0.20
mg/kg/min following an IV loading dose of 0.5
mg/kg) and constant rate infusions of ketamine (1 mg/kg/hr) and medetomidine
(1.25 µg/kg/hr). The horses were anesthetized for 175 ± 14 min (range
from 160 to 197 min). Propofol infusion rates ranged from 0.13 to 0.17 mg/kg/min, and
plasma concentration (Cpl) of propofol ranged from 11.4 to 13.3
µg/ml during surgery. Cardiovascular measurements
during surgery remained within clinically acceptable ranges in the horses (heart rate: 33
to 37 beats/min, mean arterial blood pressure: 111 to 119 mmHg, cardiac index: 48 to 53
ml/kg/min, stroke volume: 650 to 800 ml/beat and
systemic vascular resistance: 311 to 398 dynes/sec/cm5). The propofol Cpl
declined rapidly after the cessation of propofol infusion and was significantly lower at
10 min (4.5 ± 1.5 µg/ml), extubation (4.0 ± 1.2
µg/ml) and standing (2.4 ± 0.9
µg/ml) compared with the Cpl
at the end of propofol administration (11.4 ± 2.7
µg/ml). All the horses recovered uneventfully and stood
at 74 ± 28 min after the cessation of anesthesia. KMP-TIVA provided satisfactory quality
and control of anesthesia with minimum cardiovascular depression in horses undergoing
surgery. 相似文献
103.
Seiya MAEHARA Yoshiki ITOH Sho HOSHINO Miri HAYASHI Yosuke ITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1335-1338
The purpose of this study was to evaluate the dark adaptation time in canine
electroretinography (ERG) using a contact lens electrode with a built-in LED. Twelve eyes
of six normal laboratory beagle dogs were used and exposed to steady room light at 500 lux
for 30 min for light adaption. ERG was recorded at different time points during dark
adaptation in sedated and light-adapted beagles. The stimulus intensity was 0.0096
cd/m2/sec. The b-wave amplitude increased significantly until 25 min of dark
adaptation, whereas no significant changes in amplitudes were observed after 30 min. Dark
adaptation for more than 25 min would be necessary for accurate ERG in canine ERG using a
contact lens electrode with a built-in LED. 相似文献
104.
New canine parvovirus 2a infection in an imported Asian small-clawed otter (Aonyx cinereus) in Japan
Kenichi TAMUKAI Shohei MINAMI Sho KADEKARU Ikki MITSUI Ken MAEDA Yumi UNE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):507
Post-import from the Republic of Indonesia to Japan in 2017, two juvenile, captive bred Asian small-clawed otters (Aonyx cinereus) exhibited gastrointestinal symptoms, including vomiting, diarrhea, and hematemesis, and died. One of them was examined postmortem. Microscopically, the small intestinal mucosa was necrotic with crypts lined by regenerating large epithelial cells. A gastric cardiac mucosal ulcerative lesion containing fungal yeasts and pseudohyphae morphologically indicated Candida spp. The lymph nodes exhibited marked lymphoid depletion. Canine parvovirus 2 (CPV-2) was isolated from an oral swab, and virus protein 2 (VP2) gene sequencing revealed new CPV-2a. To our knowledge, this is the first new CPV-2a infection report in Asian small-clawed otters. This infection should be considered in gastrointestinal symptom-related cases in this species. 相似文献
105.
Yasuyuki Morimoto Patrick Maundu Makoto Kawase Hiroshi Fujimaki Hiroko Morishima 《Genetic Resources and Crop Evolution》2006,53(5):963-974
The present study was performed to investigate genetic diversity of Kenyan landraces of the white-flowered gourd (Lagenaria siceraria), which exhibits tremendous morphological variation. RAPD analyses were performed on 53 landraces of the cultivated species
L. siceraria and 42 accessions of three wild species (40 L. sphaerica, 1 L. abyssinica, and 1 L. breviflora). A total of 432 polymorphic bands were detected using 54 primers. The four species were clearly differentiated from one
another. Intra-specific variations were investigated with L. siceraria and its wild relative L. sphaerica. Landraces of the cultivated species collected from different ethnic communities or regions were differentiated. Morphological
variations were not associated with RAPD variations. Bitter landraces collected in Maasai communities showed two specific
RAPD bands. In the wild species, accessions collected from the eastern and western sides of the Great Rift Valley were genetically
differentiated from each other. In both species, genetic and geographical distance matrices computed among all pairs of accessions
were significantly correlated, implying that the observed geographical variation can be explained by the 'Isolation by distance
model'. Progeny plants derived from a common mother in L. siceraria showed a low level of segregation in RAPD pattern, suggesting that collected landraces are cultivated, maintaining their
inherent traits although they are monoecious and insect-pollinated, whereas the wild relative L. sphaerica showed a higher level of segregation. The morphological diversity observed among landraces of L. siceraria is the result of human selection and their genetic identities are maintained by inbreeding probably resulting from frequent
self-pollination. 相似文献
106.
Levy GS Linfield RP Ulvestad JS Edwards CD Jordan JF DI Nardo SJ Christensen CS Preston RA Skjerve LJ Stavert LR Burke BF Whitney AR Cappallo RJ Rogers AE Blaney KB Maher MJ Ottenhoff CH Jauncey DL Peters WL Nishimura T Hayashi T Takano T Yamada T Hirabayashi H Morimoto M Inoue M Shiomi T Kawaguchi N Kunimori H 《Science (New York, N.Y.)》1986,234(4773):187-189
An orbiting spacecraft and ground observatories have been used to obtain interferometric observations of cosmic radio sources. The Tracking and Data Relay Satellite System (TDRSS) was used as the orbiting observatory in conjunction with two 64- meter radio telescopes at ground observatories, one in Australia and one in Japan. The quasars 1730-130 (NRAO 530), 1510-089, and 1741-038 were observed at a frequency of 2.3 gigahertz, and a maximum projected baseline of 1.4 earth diameters was achieved. All quasar observations for which valid data were acquired resulted in detected fringes. Many of the techniques proposed for a dedicated very long baseline interferometry observatory in space were used successfully in this experiment. 相似文献
107.
Morimoto M Tanimoto K Nakano S Ozaki T Nakano A Komai K 《Journal of agricultural and food chemistry》2003,51(2):389-393
The antifeedant polymethylated flavones 5-hydroxy-3,6,7,8,4'-heptamethoxyflavone, 5-hydroxy-3,6,7,8-tetramethoxyflavone, and 5,6-dihydroxy-3,7-dimethoxyflavone have been isolated from the cudweed, Gnaphalium affine D. Don (Compositae). These flavonoids and authentic analogues showed insect antifeedant activity against the common cutworm (Spodoptera litura F.). In a previous paper, it was suggested that there was no substituent on the B-ring of the flavonoid for the beneficial antifeedant activity against the common cutworm. These flavonoids having a phenyl group as the B-ring and the chromone as elimination of the B-ring from the flavonoids were used to test the hypothesis of the previously described B-ring effect. The known fact is that Sculletaria baicarensis (Rutaceae) produced the 2-phenyl flavone. Test compounds and their methylated derivatives were prepared from this material for the structure-activity relationship (SAR) study of insect antifeedant activity. In spite of the 2-phenyl flavonoids, some tested compounds did not show any insect antifeedant activity against the common cutworm, although these inactive flavonoids were deficient in the 6-substituent group on the A-ring of the flavonoid. This 6-position-substituted derivative almost showed strong insect antifeedant activity against common cutworm. Moreover, the tested flavonoids having a hydroxyl group as a substituent on any of the positions tended to increase the activity. These results suggested the importance of the 6-position substitution on the flavonoid; however, hydrophilic substituents decreased the activity. Baicalein (5,6,7-trihydroxyflavone) derivatives did not show any activity despite having the 6-substituent derivative. Although the activity of some chromones increased the activity of the flavone, the bulky B-ring was a disadvantage for the antifeedant activity. It was suggested that the charge on C(3) and C(5) of the flavonoid was important for the biological activity. Additionally, an adequate hydrogen bonding property, which is different from lipophilicity, was an advantage for the activity on the basis of a QSAR analysis. 相似文献
108.
109.
110.
Forest buffer zones have recently been introduced in an attempt to suppress debris movement caused by mass wasting. There
are, however, many questions left unsolved regarding the role of forests in suppressing debris movement and damage to forests
by debris deposition. A number of debris avalanches occurred in southern Fukushima Prefecture and Hiroshima Prefecture in
August 1998 and June 1999, respectively. Suppression of debris movement by forests and damage to forests by debris deposition
were investigated in this study using aerial photographic interpretation and topographic analysis. Of the debris avalanches
delineated in the Fukushima and Hiroshima areas, 282 and 84 sites, respectively, were forested, and 43 and seven sites, respectively,
were nonforested. Topographic parameters, land use, and forest type at each site were surveyed by aerial photographic interpretation
and topographic analysis. Suppression of debris movement by forest vegetation was confirmed by higher equivalent coefficients
of friction and shorter average deposition zone length (about 28% and 55% shorter in the Fukushima and Hiroshima areas, respectively)
in forested sites compared with nonforested sites. While previous studies suggested that the width of the forest zone required
to prevent sediment outflow by surface erosion increases with increasing slope gradient, no clear relationship was found in
this study. While the length of deposition zone of debris material (this study) is strongly affected by the dynamic solid
friction coefficient and fluid friction of debris material, the length of deposition zone of the outflow of surface wash (past
studies) is mainly affected by the tractive force of water flow. Among the 65 trees that remained at the terminus of deposition
zones at five sites, 36 were alive and 29 were dead. Damage to the forest increases with increasing thickness of deposits
and decreases with increasing tree diameter. Such results are useful for designing forest buffer zones.
Received: June 18, 2001 / Accepted: August 21, 2002
Correspondence to:Y. Ishikawa 相似文献