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211.
Draft genome sequences have been determined for the soybean pathogen Phytophthora sojae and the sudden oak death pathogen Phytophthora ramorum. O?mycetes such as these Phytophthora species share the kingdom Stramenopila with photosynthetic algae such as diatoms, and the presence of many Phytophthora genes of probable phototroph origin supports a photosynthetic ancestry for the stramenopiles. Comparison of the two species' genomes reveals a rapid expansion and diversification of many protein families associated with plant infection such as hydrolases, ABC transporters, protein toxins, proteinase inhibitors, and, in particular, a superfamily of 700 proteins with similarity to known o?mycete avirulence genes.  相似文献   
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Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV), has been identified as a mechanically transmitted potexvirus (Alphaflexiviridae). In Argentina, cassava is grown mainly in the northeast (NEA) region that shares borders with Brazil and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated with severe leaf mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT‐PCR and sequencing. In this work, the virus has been successfully purified and a double‐antibody sandwich (DAS‐) ELISA test has been developed from an Argentinean isolate of CsCMV to extend the diagnostics of the disease. A collection of 726 samples was screened and CsCMV was detected with 100% prevalence in the NEA region. Additional co‐infecting viruses were detected in some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of CsCMV isolates. Interestingly, a separate phylogenetic cluster was formed by isolates from the NEA region that only shared 77.1% to 80.3% nucleotide identity with the other clusters. These results indicate the presence of mixed strains occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America.  相似文献   
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The growth‐promoting rhizobacteria Azospirillum brasilense Sp7 positively affects many crops, but its influence on barley remains to be fully investigated. The aim of this study was to track early root growth of four barley cultivars that are widely used in Spanish breeding programmes under different growing scenarios. Different growth conditions are hypothesized to affect the response of young plants to A. brasilense Sp7, so seeds were inoculated with A. brasilense Sp7 and directly sown in the growth chamber, or planted in vitro. Plant height was measured and root structure analysed with the WinRHIZO program. Azospirillum brasilense Sp7 inoculation increased the length, surface area and number of root tips in both systems and in most cultivars in a similar way. Cultivars ‘Barberousse’ and ‘Plaisant’ were the most responsive to A. brasilense Sp7 treatment, while ‘Albacete’ was especially interesting in terms of its physiological interaction with A. brasilense Sp7 under abiotic stress imposed by the in vitro system. The utility of the in vitro system is criticized.  相似文献   
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Erythromycin is one of the most widely used antibiotics globally and is considered an emerging contaminant in wastewaters and environmental waters. The egg, larval, and pupal stages of the dengue vector Aedes aegypti L. reside and develop in aquatic environments. These mosquito stages may be exposed to compounds in the water, such as erythromycin. Aquatic stages of Ae. aegypti were reared in different concentrations of erythromycin which resulted in significant delay and decrease in eclosion of eggs and pupation of larvae (P?<?0.05). Moreover, emergence of adults from pupae, larval survival, and adult female fecundity significantly decreased (P?<?0.05). A few occurrences of hypopigmentation in larvae and blisters in adult mosquitoes were also observed. Interestingly, second-generation eggs, which were laid by adult female mosquitoes exposed to erythromycin during their aquatic stages, did not exhibit decreased levels of eclosion in the presence of erythromycin. These results reveal that long-term erythromycin exposure disrupts the Ae. aegypti life cycle by decreasing survival and delaying progression throughout different life stages. However, this study demonstrates that Ae. aegypti can rapidly acquire significant tolerance to the emerging environmental contaminant erythromycin within two generations.  相似文献   
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