全文获取类型
收费全文 | 14069篇 |
免费 | 615篇 |
国内免费 | 1527篇 |
专业分类
林业 | 1664篇 |
农学 | 2113篇 |
基础科学 | 1139篇 |
2290篇 | |
综合类 | 4206篇 |
农作物 | 890篇 |
水产渔业 | 481篇 |
畜牧兽医 | 1864篇 |
园艺 | 588篇 |
植物保护 | 976篇 |
出版年
2024年 | 37篇 |
2023年 | 159篇 |
2022年 | 407篇 |
2021年 | 624篇 |
2020年 | 514篇 |
2019年 | 520篇 |
2018年 | 328篇 |
2017年 | 459篇 |
2016年 | 482篇 |
2015年 | 663篇 |
2014年 | 570篇 |
2013年 | 724篇 |
2012年 | 866篇 |
2011年 | 860篇 |
2010年 | 846篇 |
2009年 | 815篇 |
2008年 | 764篇 |
2007年 | 812篇 |
2006年 | 754篇 |
2005年 | 670篇 |
2004年 | 346篇 |
2003年 | 313篇 |
2002年 | 252篇 |
2001年 | 262篇 |
2000年 | 301篇 |
1999年 | 379篇 |
1998年 | 355篇 |
1997年 | 310篇 |
1996年 | 286篇 |
1995年 | 251篇 |
1994年 | 229篇 |
1993年 | 173篇 |
1992年 | 191篇 |
1991年 | 192篇 |
1990年 | 138篇 |
1989年 | 113篇 |
1988年 | 103篇 |
1987年 | 44篇 |
1986年 | 33篇 |
1985年 | 9篇 |
1984年 | 14篇 |
1983年 | 11篇 |
1982年 | 7篇 |
1981年 | 6篇 |
1980年 | 8篇 |
1962年 | 2篇 |
1925年 | 1篇 |
1905年 | 1篇 |
1896年 | 2篇 |
1864年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 5 毫秒
101.
Xiangbing Mao Mei Lv Bing Yu Jun He Ping Zheng Jie Yu Quyuan Wang Daiwen Chen 《畜牧与生物技术杂志(英文版)》2014,5(1)
Oxidative stress can induce abnormal tryptophan metabolism. The present study was mainly conducted to determine the effect of dietary tryptophan levels on oxidative stress in the liver of weaned pigs challenged by diquat. A total of 36 PIC piglets weaned at 21 days of age were randomly allotted to 1 of 3 diets containing dietary tryptophan levels of 0.18, 0.30, and 0.45% for 14 d. On day 8, the piglets were injected intraperitoneally with sterile 0.9% NaCl solution or diquat (10 mg/kg body weight). During the first 7 d of trial, increasing dietary tryptophan levels enhanced average daily gain (P = 0.09) and average daily feed intake (P = 0.08), and decreased the feed efficiency (P < 0.05) of piglets. The growth performance was decreased by diquat injection (P < 0.05). Diquat injection also decreased the activities of the superoxide dismutase (SOD) and glutathione peroxidase (GPx) in the plasma and liver (P < 0.05), increased plasma malondialdehyde (MDA) (P < 0.05) and urea nitrogen (P < 0.05) concentrations, and enhanced MDA concentration (P = 0.09) and tryptophan 2,3-dioxygenase (TDO) activity (P = 0.07) in liver of piglets. Increasing dietary tryptophan levels could attenuate the effects of diquat injection on the MDA (P = 0.06) concentration and the activities of SOD (P = 0.09) and GPx (P = 0.05) of the liver, and plasma urea nitrogen (P = 0.06) concentration in the piglet. There was a synergistic role for increasing TDO activity in the liver between dietary tryptophan levels and diquat injection (P < 0.05). These results suggest that increasing dietary tryptophan levels could attenuate the oxidative stress of the liver in weaned piglets intraperitoneally injected with diquat via enhancing the antioxidant capacity. 相似文献
102.
牛超数排卵技术的研究 总被引:11,自引:1,他引:11
用FSH+PGF1。超排处理72头次供体牛,采卵361枚,可用胚235枚,黄牛胚胎可用率高于奶牛、肉牛,育成牛胚胎可用用率高于经产牛,秋冬季超排效果明显好于春季。 相似文献
103.
一辽宁省畜牧业发展现状
1主要畜产品产量增长迅速.畜牧业产值稳步提升.畜产品外销势头强劲
改革开放以来,辽宁省肉类总产量的年平均增长速度为7.4%。2008年末,辽宁肉类总产量为376万吨,比1978年增加7.5倍。禽蛋和牛奶产量增长速度更快,1978年以来年均增速达到10.5%和10.2%, 相似文献
104.
105.
Inhibition of porcine circovirus type 1 and type 2 production in PK-15 cells by small interfering RNAs targeting the Rep gene 总被引:1,自引:0,他引:1
Porcine circovirus type 1 (PCV1) and type 2 (PCV2) are two genotypes of porcine circovirus. Both of them are presumed to be widespread in the swine population. Currently, there is no specific treatment for their infections. RNA interference (RNAi) is a sequence-specific RNA degradation mechanism mediated by small interfering RNA (siRNA), which represents a possible therapeutic application for the treatment of viral infections. In this study, three siRNA expression plasmids (pS-RepA, pS-RepB and pS-RepC) were generated to target three different coding regions of the Rep protein (Rep) of PCV. These siRNAs were used to inhibit PCV production in a porcine kidney cell line, PK-15 cells. Our results revealed that Rep gene expression was inhibited by pS-RepA, pS-RepB and pS-RepC to different degrees. Moreover, our study also showed that the production of PCV1 and PCV2 was reduced by these siRNAs. pS-RepC, which targets the middle region of Rep gene, proved to be the most efficient siRNA for inhibition of Rep expression and viral production. Taken together, our data suggest that RNAi could be investigated as a potential treatment for PCV infection. 相似文献
106.
107.
108.
多年来,畜牧营养学家一直利用酸性洗涤纤维(ADF)和中性洗涤纤维(NDF)作为饲料能量和采食量的指示剂,特别是在反刍日粮中。 相似文献
109.
Studies of the response of the primary avian polymorphonuclear leukocyte, the heterophil, to microbe associated molecular patterns (MAMPs) through toll-like receptors (TLR) has concentrated on the activation of the respiratory burst, release of intracellular granules, and the induction of cytokine and chemokine expression. Virtually no studies have been described on the role of lipid mediators, leukotrienes and prostaglandins, as effectors of the avian inflammatory response. We have previously shown that flagellin (FLG), the bacterial lipoprotein mimic palmitoly-3-cysteine-serine-lysine-4 (PAM), and unmethylated CpG motifs of bacteria DNA (CpG) are all potent activators of the avian innate immune system. In the present studies, we hypothesized that FLG, PAM, and CpG are also capable of eliciting the production of these lipid mediators of inflammation by avian heterophils. Compared to non-stimulated control heterophils, all three TLR agonists were potent inducers (3-5-fold increase) of a rapid production (30 min) of leukotriene B(4) (LTB(4)) followed by a later release (60-120 min) of prostaglandin (PGE(2)) by the heterophils. LTB(4) and PGE(2) production were derived from lipoxygenase-5 (5-LO) and cyclooxygenase-2 (COX-2) enzymatic activities, respectively, as the selective 5-LO (caffeic acid) and COX-2 (NS-398) inhibitors eliminated LTB(4) and PGE(2) production from the MAMP-stimulated heterophils. These results demonstrate that both the lipoxygenase and cycloxygenase pathways are operational in avian heterophils in response to bacterial MAMPs. Treatment of heterophils with either FLG, PAM, or CpG also induced a significant increase in DNA binding by NF-κB family members' p50, c-Rel, and RelB. Additionally, the production of LTB(4) and PGE(2) were inhibited following treatment of heterophils with the specific pharmacologic inhibitor of NF-κB (Bay 11-7086), thus suggesting that TLR pathway activation of NF-κB controls LTB(4) and PGE(2) production. This the first report of the production of lipid mediators of inflammation by avian heterophils in response to PAMPs. Since FLG, lipoproteins, and bacterial CpG DNA are abundant during bacterial infections, these data support their role in the inflammatory response mediated by avian heterophils. 相似文献
110.
生物毒素灭治草原害鼠研究及其应用 总被引:1,自引:0,他引:1
用 C 型肉毒梭菌经过滤除菌的外毒素作为杀鼠剂,并用浸泡法配制成各种浓度的毒素毒饵,系灭草原害鼠。此杀鼠剂用药量少、灭鼠效果好、而且成本低、残效期短、不污染环境、无二次中毒、无“三致”作用,对人畜安全、使用方便、生产简单。毒素杀鼠剂的使用为国内外首创,在我国推广面积已达227万公顷,深受群众欢迎,取得了极好的经济效益和社会效益。 相似文献