Immune responses in piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus

Porcine reproductive and respiratory syndrome virus (PRRSV) infection compromises the host's innate and adaptive immunity. The aim of this study was to investigate the immune responses of piglets infected with highly pathogenic (HP) PRRSV (HuN4 strain) with or without the immunization with CH-1R attenuated PRRSV vaccine. The response was evaluated for the clinical signs, pathological changes and virus load in immune organs, antibody responses and levels of serum IFN-γ, IL-4 and IL-10. The result showed that in comparison with the piglets received the immunization, the piglets infected with HP-PRRSV alone had the thymus atrophy, decreased serum levels of IL-4 and increased serum levels of IL-10 and INF-γ. These results suggest that elevated IL-10 levels at the early stage of the infection may enhance virus survival and delay the induction of protective immunity, while increased levels of IL-4 induce the effective immune responses and increase the animals' health status.     

东方百合L_sCCR1基因的克隆及表达分析

百合茎秆的挺度直接关系到百合花采收后的货架寿命等重要商品性状,而这些商品性状与植物茎秆中的木质素的含量高低有关.本文应用RLM-RACE技术,从东方百合索蚌植株中克隆了木质素合成相关基因一肉桂酰辅酶A还原酶,定名为LsCCR1.该基因cDNA全长为1 499 bp,包括完整的阅读框,编码388个氨基酸.多重比对分析发现百合LsCCR1基因与其他植物上已经发现的CCR基因同源性多介于55%～66%之间.利用半定量PCR检测LsCCR1基因在百合不同组织中的表达差异,结果显示LsCCR1基因主要在百合的茎秆中表达,根部次之,叶片、鳞茎及花蕾中表达量较少,其表达模式与拟南芥、桉树及大麦中CCR基因的表达模式类似.     

黑茶在中国茶业史上的意义——郭红军著《黑茶通史——兼记民国茶事》述评

郭红军先生所著《黑茶通史——兼记民国茶事》一书,是一部研究中国黑茶的力作。其重要的学术价值在于阐述了黑茶生产和饮用的悠久历史及黑茶在中国民族史上所起过的重要作用,并介绍了中国各地域黑茶生产的独特技术和中国各地域黑茶的内销和外销。     

加强专项管理加快专项预算执行进度

该文阐述了切实加强专项管理工作领导的重要性,强调要落实责任,加快专项预算执行进度,提出从业人员要加强学习与培训,努力提高业务素质,切实做好2007年、2008年修购项目验收工作及2010年修购项目实施的前期准备工作。     

新疆克拉玛依市兴农湖剖面古沉积环境分析

粒度是沉积物中各种粗细颗粒的机械组成,可以用于沉积环境类型的识别和物质运动方式的判定。通过对克拉玛依市兴农湖剖面的粒度数据测量分析,判定该地区沉积物为湖相沉积。联系周围区域构造形态,该地区应为古玛纳斯湖的湖盆地区。     

海南水牛血液蛋白多态性研究

采用醋酸纤维薄膜、聚丙烯酰胺凝胶电泳和淀粉凝胶电泳对９７头海南水牛的血红蛋白、血清运铁蛋白和白蛋白多态性进行检测，并计算其基因型和基因频率，发现６头血红蛋白三条变异体和１头一带变异体。     

DNA Barcoding:An Effective Tool for Species Identification of Lepidopteran Oak Pests

[Objective] The paper was to improve the efficiency and accuracy of early forecast of Lepidopteran oak-infesting pests.[Method] DNA barcoding technique was established for quick species identification using mitochondrial cytochrome C oxidase subunit Ⅰ(COⅠ) as the standard gene.This barcoding technique was used to amplify and sequence genomic DNA samples from eggs and pupae of 11 species of Lepidopteran pests collected from oak.[Result] The DNA barcoding standard genes of 594-708 bp were determined from eggs and pupae of Lepidopteran insects.There were differences of 0-2 bases in DNA barcode sequences between conspecific eggs and pupae,with the sequence identity of 99.7%-100%.The average content of A,T,G and C of DNA barcode sequences from Lepidopteran insects were 30.7%,38.5%,14.9% and 15.9%,respectively.The obtained DNA barcode sequences had 91.4%-100% identity and 0-8.6% difference degree with GenBank-deposited DNA barcode sequences from organisms of the genetically-closest relationship.Among them,DNA barcode sequences from egg and pupa samples of 10 Lepidopteran insects(No.1-20) had 99%-100% identity and 0-1.0% difference degree with homologous sequences in GenBank database,while the remaining samples(No.21-22) had high difference degree(8.6%) with homologous sequences.[Conclusion] The established DNA barcoding technique is an effeetive tool for species identification of Lepidopteran pests using genomic DNA from eggs and pupae of Lepidopteran insects.     

Expression of nerve growth factor and its receptors trkA and p75 and inhibin alpha-subunit in the ovarian interstitial cells of lactating golden hamsters

Characteristic daily increases in the plasma levels of luteinizing hormone (LH) are present every afternoon during lactation in golden hamsters. The objective of this study was to investigate the effect of the diurnal rhythm of increases in LH on expression of nerve growth factor (NGF), its receptors trkA and p75 and inhibin alpha-subunit in the ovarian interstitial cells of lactating golden hamsters. Both lactating and non-lactating groups of postpartum golden hamsters were used in this study. The expression of NGF, its receptors trkA and p75 and inhibin alpha-subunit were determined by immunohistochemistry. Positive staining of NGF, trkA and p75 was found in the interstitial cells of the lactating group, and no immunoreactivity for NGF, trkA or p75 was observed in the ovarian interstitial cells of the non-lactating group. In addition, immunostaining of inhibin alpha-subunit was also observed in the interstitial cells of the lactating group but not in those of the non-lactating group. Immunostaining of the inhibin/activin beta(A)- and beta(B)-subunits was observed in the granulosa cells of antral follicles, but not in the interstitial cells of the lactating and non-lactating animals. These results suggest that the diurnal rhythm increases in LH can induce expression of NGF, trkA, p75 and inhibin alpha-subunit in the ovarian interstitial cells of lactating golden hamsters and that NGF, its receptors trkA and p75 and inhibin alpha-subunit may have the capacity for autocrine or paracrine modulation of interstitial cell differentiation in golden hamsters.     

烟碱对大鼠心电图的影响

烟碱对心率的作用具有阶段性,初期引起心率短暂性减弱,紧接着发生瞬时增加。为进一步确定烟碱对大鼠心脏的作用,对描记烟碱作用后大鼠心电图进行分析,发现烟碱能引起大鼠心电图显示较长的QT间期,增加了PR间期及T波面积,与肾上腺素或去甲肾上腺素引起的QT间期延长相似,且能被M受体阻断剂阿托品所阻断,说明烟碱能够诱导局部释放乙酰胆碱或者作用于部分副交感神经控制的毒蕈碱受体。