作物栽培学讲授思路和方法的探索

对作物栽培学的讲授思路和方法进行探索,在思路上,将该门课程的开始与结束由简图和详图串连起来,使知识的掌握更加系统完整;在方法上,归纳出按"位置、形状、内容"的线索,思考作物栽培学的基本概念、将作物生长发育特性的描述性语言转换成图形和公式,从感性认识进入理性思考并便于记忆。     

草地贪夜蛾核型多角体病毒对草地贪夜蛾的毒力及其侵染特征

为明确草地贪夜蛾核型多角体病毒（Spodoptera frugiperda multiple nucleopolyhedrovirus,SfMNPV）对草地贪夜蛾Spodoptera frugiperda 的毒力及其体内外侵染特性,于室内测定SfMNPV对草地贪夜蛾的毒力,采用苏木精-伊红（hematoxylin-eosin,HE）染色分析SfMNPV侵染后草地贪夜蛾的组织病理学特征,通过实时荧光定量PCR（real-time fluorescence quantitative PCR,qPCR）技术分析SfMNPV在草地贪夜蛾体内和Sf9细胞系中的复制特征。结果表明,SfMNPV对草地贪夜蛾2龄幼虫的LC₅₀和LT₅₀分别为7.39×10⁵ OBs/mL和3.82 d;SfMNPV可侵染草地贪夜蛾幼虫的真皮、气管、脂肪体和中肠等组织,未侵染肌肉组织;SfMNPV侵染96 h后草地贪夜蛾体内SfMNPV基因组拷贝数最大,为3.91×10⁶ 拷贝数/ng,SfMNPV侵染96 h后Sf9细胞培养上清液中SfMNPV基因组拷贝数最大,为2.08×10⁷ 拷贝数/mL。     

东北玉米通风调质试验

东北地区储粮粮堆由于气候原因存在一个巨大的"冷心",冷心的主要应用方式为膜下环流均温控制和缓式通风上调冷心控粮温,这两种方式都是利用冷资源进行的粮温控制。而目前的粮堆增湿方式主要为湿膜机增湿和各种调质机增湿,利用"冷心"增湿还没有成功的案例。利用冷心增湿,内结露现象很严重,而且容易出现局部水分偏高现象,且违反机械通风操作规程1。但通过缓式通风的精确掌握和粮堆增湿理论的精确控制,大胆试验,事实证明冷心内结露增湿方式是可行的。试验将出仓前平均水分13.5%提高到最高14.8%,平均水分14.1%,达到试验预期目的。试验仓出仓验证实际增量21.05t,效益十分可观。     

Immune responses in piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus

Porcine reproductive and respiratory syndrome virus (PRRSV) infection compromises the host's innate and adaptive immunity. The aim of this study was to investigate the immune responses of piglets infected with highly pathogenic (HP) PRRSV (HuN4 strain) with or without the immunization with CH-1R attenuated PRRSV vaccine. The response was evaluated for the clinical signs, pathological changes and virus load in immune organs, antibody responses and levels of serum IFN-γ, IL-4 and IL-10. The result showed that in comparison with the piglets received the immunization, the piglets infected with HP-PRRSV alone had the thymus atrophy, decreased serum levels of IL-4 and increased serum levels of IL-10 and INF-γ. These results suggest that elevated IL-10 levels at the early stage of the infection may enhance virus survival and delay the induction of protective immunity, while increased levels of IL-4 induce the effective immune responses and increase the animals' health status.     

东方百合L_sCCR1基因的克隆及表达分析

百合茎秆的挺度直接关系到百合花采收后的货架寿命等重要商品性状,而这些商品性状与植物茎秆中的木质素的含量高低有关.本文应用RLM-RACE技术,从东方百合索蚌植株中克隆了木质素合成相关基因一肉桂酰辅酶A还原酶,定名为LsCCR1.该基因cDNA全长为1 499 bp,包括完整的阅读框,编码388个氨基酸.多重比对分析发现百合LsCCR1基因与其他植物上已经发现的CCR基因同源性多介于55%～66%之间.利用半定量PCR检测LsCCR1基因在百合不同组织中的表达差异,结果显示LsCCR1基因主要在百合的茎秆中表达,根部次之,叶片、鳞茎及花蕾中表达量较少,其表达模式与拟南芥、桉树及大麦中CCR基因的表达模式类似.     

黑茶在中国茶业史上的意义——郭红军著《黑茶通史——兼记民国茶事》述评

郭红军先生所著《黑茶通史——兼记民国茶事》一书,是一部研究中国黑茶的力作。其重要的学术价值在于阐述了黑茶生产和饮用的悠久历史及黑茶在中国民族史上所起过的重要作用,并介绍了中国各地域黑茶生产的独特技术和中国各地域黑茶的内销和外销。     

加强专项管理加快专项预算执行进度

该文阐述了切实加强专项管理工作领导的重要性,强调要落实责任,加快专项预算执行进度,提出从业人员要加强学习与培训,努力提高业务素质,切实做好2007年、2008年修购项目验收工作及2010年修购项目实施的前期准备工作。     

新疆克拉玛依市兴农湖剖面古沉积环境分析

粒度是沉积物中各种粗细颗粒的机械组成,可以用于沉积环境类型的识别和物质运动方式的判定。通过对克拉玛依市兴农湖剖面的粒度数据测量分析,判定该地区沉积物为湖相沉积。联系周围区域构造形态,该地区应为古玛纳斯湖的湖盆地区。     

DNA Barcoding:An Effective Tool for Species Identification of Lepidopteran Oak Pests

[Objective] The paper was to improve the efficiency and accuracy of early forecast of Lepidopteran oak-infesting pests.[Method] DNA barcoding technique was established for quick species identification using mitochondrial cytochrome C oxidase subunit Ⅰ(COⅠ) as the standard gene.This barcoding technique was used to amplify and sequence genomic DNA samples from eggs and pupae of 11 species of Lepidopteran pests collected from oak.[Result] The DNA barcoding standard genes of 594-708 bp were determined from eggs and pupae of Lepidopteran insects.There were differences of 0-2 bases in DNA barcode sequences between conspecific eggs and pupae,with the sequence identity of 99.7%-100%.The average content of A,T,G and C of DNA barcode sequences from Lepidopteran insects were 30.7%,38.5%,14.9% and 15.9%,respectively.The obtained DNA barcode sequences had 91.4%-100% identity and 0-8.6% difference degree with GenBank-deposited DNA barcode sequences from organisms of the genetically-closest relationship.Among them,DNA barcode sequences from egg and pupa samples of 10 Lepidopteran insects(No.1-20) had 99%-100% identity and 0-1.0% difference degree with homologous sequences in GenBank database,while the remaining samples(No.21-22) had high difference degree(8.6%) with homologous sequences.[Conclusion] The established DNA barcoding technique is an effeetive tool for species identification of Lepidopteran pests using genomic DNA from eggs and pupae of Lepidopteran insects.     

Expression of nerve growth factor and its receptors trkA and p75 and inhibin alpha-subunit in the ovarian interstitial cells of lactating golden hamsters

Characteristic daily increases in the plasma levels of luteinizing hormone (LH) are present every afternoon during lactation in golden hamsters. The objective of this study was to investigate the effect of the diurnal rhythm of increases in LH on expression of nerve growth factor (NGF), its receptors trkA and p75 and inhibin alpha-subunit in the ovarian interstitial cells of lactating golden hamsters. Both lactating and non-lactating groups of postpartum golden hamsters were used in this study. The expression of NGF, its receptors trkA and p75 and inhibin alpha-subunit were determined by immunohistochemistry. Positive staining of NGF, trkA and p75 was found in the interstitial cells of the lactating group, and no immunoreactivity for NGF, trkA or p75 was observed in the ovarian interstitial cells of the non-lactating group. In addition, immunostaining of inhibin alpha-subunit was also observed in the interstitial cells of the lactating group but not in those of the non-lactating group. Immunostaining of the inhibin/activin beta(A)- and beta(B)-subunits was observed in the granulosa cells of antral follicles, but not in the interstitial cells of the lactating and non-lactating animals. These results suggest that the diurnal rhythm increases in LH can induce expression of NGF, trkA, p75 and inhibin alpha-subunit in the ovarian interstitial cells of lactating golden hamsters and that NGF, its receptors trkA and p75 and inhibin alpha-subunit may have the capacity for autocrine or paracrine modulation of interstitial cell differentiation in golden hamsters.