小麦对条锈病的水平抗病性研究初报

将小麦品种采用随机区组的田间设计种于小种病圃中,以鉴定和测定其水平抗性。根据各个“品种——小种”组合的相对病情指数的方差分析的结果,有些品种,如农大311、西农6028和丰产3号,其品种——小种互作高度显著,被鉴定为具有垂直抗性,另外一些品种,对试验所用的小种并无专化性,并且在大田生产中已显出20多年的持久的中度抗性,初步推测为属水平抗性。在后一类中,有些品种,如平原50,表现为中等反应型,其余则属于呈典型感病的迟锈慢锈类型。在迟锈慢锈品种的病指的对数矫正值(logits)和病圃的锈病强度(以感病对照品种的病指表之)的对数矫正值之间发现了直线回归。     

履带板用高锰钢强韧化研究

本文根据履带板的失效特征和机理,提出了高锰钢的铬、钼再合金化,稀土、钛变质处理和重结晶热处理工艺综合强韧化方案.经实验证实,综合机械性能显著改善.     

‘Plantibodies’: a flexible approach to design resistance against pathogens

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.     

早生桑品种育151、育237的育成

采用杂交方法育成早生桑品种育151、育237,具有发芽早、产叶量高,叶质较优、抗黄化型萎缩病较强等特性,发芽期比早青桑捉早2-3天.比湖桑32号早8天左右.产叶量提高6-41%.     

水稻品种对稻褐飞虱抗性的研究

水稻品种对褐飞虱的不同生物型反应是不相同的。试验结果证实:(1)褐飞虱若虫在敏感性品种上的生存率显著地高于抗性品种。在TN_1上,无论那种生物型的生存率均很高;而栖息在Mudgo的褐飞虱若虫,其生存率以生物型2的最高,而生物型1与生物型3的均很低,栖息在ASD_7的若虫,以生物型3的生存率最高。(2)褐飞虱在敏感品种上的后代群体密度显著地比抗性品种的大。生活在敏感品种的若虫,发育进度较抗性品种的快,并且身体较重。(3)在敏感品种取食的飞虱,其分泌的蜜露量较抗性品种的多,生物型2取食Mudgo的和生物型3取食ASD_7的均分泌较多的蜜露。上述结果说明了水稻品种抗褐飞虱的机制属抗生性范畴。     

不同施肥期沾化冬枣对15N的吸收、分配及利用特性

 盆栽条件下利用¹⁵N示踪技术, 研究不同时期施15N - 尿素, 对沾化冬枣¹⁵N的吸收利用及分配特性的影响。结果表明: 生长季前期(萌芽前和花前) 施用¹⁵N - 尿素, 经根系吸收后, ¹⁵N优先分配到贮藏器官(包括主干、多年生枝和粗根) 中, 然后外运用于树体新生器官(包括枣吊及其叶片、新生营养枝、细根及果实) 的形成, 果实采收后¹⁵N开始向贮藏器官回流; 果实硬核期¹⁵N直接用于树体营养生长和生殖生长, 而不是先贮藏再利用; 果实速长期¹⁵N优先向贮藏器官中积累; 萌芽前施¹⁵N在树体内的运转规律符合落叶果树贮藏N营养分配规律, 优先转运到生长中心。随着施肥期的后延, 植株对¹⁵N - 尿素的当季利用率逐渐下降。     

果树病毒检测方法研究进展

综述了苹果、樱桃、李等果树病毒检测的新进展，同时对各种病毒检测方法的优缺点进行了比较。     

蔬菜连作障碍防治的研究动态

本文论述了引起蔬菜连作障碍的物理原因和生物原因,论述了包含培育抗性品种、作物嫁接、轮作倒茬、土壤消毒、土壤改良和施肥管理、化学防治、生物防治、植物检疫几种防治措施等方面的内容。本文还汇总了国内外近年来对蔬菜连作障碍的研究动态和进展,为进一步研究提供了大量的资料和参考文献。