Determining suitability of thermal development models to estimate temperature parameters for embryonic development of <Emphasis Type="Italic">Sitona lepidus</Emphasis> Gyll. (Coleoptera: Curculionidae)

The clover root weevil, Sitona lepidus Gyll. is a pest of clovers (Trifolium spp.), particularly white clover (T. repens). Larva feeding severely impairs the capacity of white clover to fix atmospheric nitrogen, by attacking the nitrogen-fixing root nodules. Development of the egg stage was studied at six different constant temperatures ranging from 9 to 30°C to improve the basis for phenological forecasts. Development occurred over the entire range of temperatures, although hatching percentage at 30°C was only 24.4% compared to 90.1% at 28.5°C. Developmental time decreased with increase in temperature from 9 to 28.5°C but increased markedly at 30°C. At 28.5°C, the embryo development period was the shortest (10.35 days); whereas at 9°C, this parameter was the longest (55.08 days). Using linear regression, the lower threshold temperature was calculated 4.38°C and the thermal constant was 236.45 degree-days. The relationship between constant temperature and developmental rate was evaluated using nine models. The suitability of the models was evaluated based on eight indicators (R ², RSS, R _adj², AIC, BIC, AICC, W _j , and Z _i ). Of the nonlinear models, the Logan-6, Sharpe and DeMichele, and Lactin models were the most accurate at calculating t _opt of 27, 27.50, and 28°C, respectively. The results suggest that of the three models (Lactin, Briere, and Hilbert and Logan), that estimated critical temperatures, (t _min, t _opt, and t _max), Lactin gave the most suitable fit of data. This response of S. lepidus to temperature can be used for developing phonological models to predict the timing of egg hatch which are important for management programs targeting the weevil.     

FTIR-ATR-based prediction and modelling of lignin and energy contents reveals independent intra-specific variation of these traits in bioenergy poplars

Background  

There is an increasing demand for renewable resources to replace fossil fuels. However, different applications such as the production of secondary biofuels or combustion for energy production require different wood properties. Therefore, high-throughput methods are needed for rapid screening of wood in large scale samples, e.g., to evaluate the outcome of tree breeding or genetic engineering. In this study, we investigated the intra-specific variability of lignin and energy contents in extractive-free wood of hybrid poplar progenies (Populus trichocarpa × deltoides) and tested if the range was sufficient for the development of quantitative prediction models based on Fourier transform infrared spectroscopy (FTIR). Since lignin is a major energy-bearing compound, we expected that the energy content of wood would be positively correlated with the lignin content.     

A simple and efficient method for isolating small RNAs from different plant species

Background  

Small RNAs emerged over the last decade as key regulators in diverse biological processes in eukaryotic organisms. To identify and study small RNAs, good and efficient protocols are necessary to isolate them, which sometimes may be challenging due to the composition of specific tissues of certain plant species. Here we describe a simple and efficient method to isolate small RNAs from different plant species.     

Diofenolan: a novel insect growth regulator in common citrus butterfly, <Emphasis Type="Italic">Papilio demoleus</Emphasis>

Penultimate and last instar larvae of the common citrus swallowtail butterfly, Papilio demoleus L. (Papilionidae: Lepidoptera), were treated with different doses of a novel juvenoid, diofenolan. Several deformities were observed as a result of topical administration of diofenolan which include delay in larval–larval and larval–pupal ecdysis, ecdysial failure, mortality, severe reduction in pupation, deformed pupae and complete inhibition of adult emergence. The juvenoid diofenolan severely hampers the normal growth, development and metamorphosis of P. demoleus and can be incorporated into integrated pest management programs for successful control of this important pest of citrus all over the world.     

Note on <Emphasis Type="Italic">Wahlgreniella nervata</Emphasis> (Gillette, 1908) (Hemiptera: Aphididae): a new pest of Damask rose in Turkey

Alate viviparous females of Wahlgreniella nervata (Gillette) were found on a Damask rose (Rosa damascena Mill.) plantation in Isparta Province, Turkey. The aphid is recorded for the first time from Turkey. Besides, Damask rose is a new host for this species. Brief information about this aphid including a key to the alate females of aphid species known to infest Damask rose in Turkey is provided.     

Resistance of <Emphasis Type="BoldItalic">Spodoptera exigua</Emphasis> to ten insecticides in Shandong,China

Spodoptera exigua (Hübner) collected from three regions (two scallion-producing regions and one ginger-producing region) in Shandong, China and a laboratory colony of Wuhan (WHLC) were evaluated for their susceptibilities to ten insecticides (emamectin benzoate, chlorfenapyr, indoxacarb, spinosyn, tebufenozide, methoxyfenozide, chlorfluazuron, beta-cypermethrin, chlorpyrifos and methomyl) in 2008, 2009 and 2010 using a leaf-dip bioassay method. The results indicate that the resistance ratios of S. exigua to newer insecticides such as emamectin benzoate, chlorfenapyr and indoxacarb were all below 20-fold, with no obvious change in all 3 years. S. exigua exhibits moderate resistance to spinosyn, and its resistance ratios increased from 1.98–5.31-fold in 2008 to 14.31–64.20-fold in 2010 from three regions as compared with WHLC. S. exigua showed moderate to high resistance to insect growth regulators such as tebufenozide, methoxyfenozide, chlorfluazuron in 2008, 2009 and 2010, in which resistance to chlorfluazuron was rapidly increased from 31.49–88.19-fold in 2008 to 1184.39–2789.67-fold in 2010. Resistance of S. exigua to beta-cypermethrin and chlorpyrifos varied greatly among the three regions, ranging from 95.31–437.97-fold and 25.05–40.64-fold in 2008, 951.81–1304.40-fold and 44.91–186.33-fold in 2009, to 27.27–1095.31-fold and 19.12–267.98-fold in 2010. In contrast, S. exigua showed low resistance to methomyl, and the resistance ratio was below 5-fold in 3 years. There are several reasons accounting for varying degrees of resistance, including selection pressure, cropping structure and migration, in which the migration of S. exigua may play an important role.     

A molecular mechanism of resistance to streptomycin in <Emphasis Type="Italic">Xanthomonas oryzae</Emphasis> pv. <Emphasis Type="Italic">oryzicola</Emphasis>

Xanthomonas oryzae pv. oryzicola, the causal agent of rice leaf streak disease, was found to be sensitive to streptomycin (an aminocyclitol glycoside antibiotic), by inhibition of protein synthesis resulting from interference with translational proofreading. This study aimed to determine the molecular resistance mechanism of X. oryzae pv. oryzicola to streptomycin. Seven streptomycin-resistant mutants were obtained by UV induction or streptomycin selection. These mutants can grow at 100 μg ml⁻¹ of streptomycin while the wild-type strain (RS105) cannot grow at 5 μg ml⁻¹. Sequencing indicated that the rpsL gene encoding ribosomal protein S12 has 375 bp encoding 125 amino acid residues. In all resistant strains, a mutation in which AAG was substituted for AGG (Lys→Arg) occurred either at codon 43 or 88. Two plasmids, pUFRRS and pUFRRX, were constructed by ligating the rpsL gene into the cosmid pUFR034. The plasmids pUFRRS and pUFRRX containing the Lys→Arg mutation of the rpsL gene conferred streptomycin resistance to the sensitive wild-type strain by electroporation. Both transformants, RS1 and RS2, could grow in the medium containing 50 μg ml⁻¹ of streptomycin. A mutation at codon 43 or 88 in rpsL can result in resistance of Xanthomonas oryzae pv. oryzicola to streptomycin.