Scratch deformations of poly (ether ether ketone) composites

The experimental results obtained from scratching a semicrystalline poly (ether ether ketone) surfaces, (PEEK), and its composites are presented in this paper. A semicrystalline PEEK and a carbon fiber oriented PEEK were scratched using conical indenters on a pendulum sclerometer. The carbon fiber oriented PEEK composites were scratched in the parallel, the orthogonal and the transverse direction to the fiber orientation. Subsequent deformations of the surfaces were assessed through subjective evaluation of the images obtained from a scanning electron microscope (SEM). The semicrystalline PEEK samples were found to be deformed by ductile ploughing and brittle deformation mechanism. In addition a fibrillation of the crystalline lamella of the polymer was also seen to be formed in case of severe brittle deformations. Fiber matrix debonding, matrix material debris formation, and fiber breakage were observed to be the dominant deformation mechanisms of the carbon fiber oriented composites. The scratch deformations of fiber oriented polymers were found to be highly dependent on fiber orientation angle relative to the scratching direction.     

Recombinant Production of a Novel Fusion Protein: Listeriolysin O Fragment Fused to S1 Subunit of Pertussis Toxin

Background:Some resources have suggested that genetically inactivated PTs bear a more protective effect than chemically inactivated products. This study aimed to produce new version of PT, by cloning an inactive PTS1 in a fusion form with N-terminal half of the LLO pore-forming toxin. Methods:Deposited pdb structure file of the PT was used to model an extra disulfide bond. Codon-optimized ORF of the PTS1 was used to make recombinant constructs of PTS1 and LLO-PTS1 in the pPSG-IBA35 vector. The recombinant PTS1 and LLO-PTS1 proteins were expressed in BL21 DE3 and SHuffle T7 strains of E. coli and purified by affinity chromatography. Cytotoxic effects of the recombinant proteins were examined in the MCF-7 cell line. Results:The purity of the products proved to be more than 85%, and the efficiency of the disulfide bond formation in SHuffle T7 strain was higher than BL21 DE3 strain. No cytotoxicity of the recombinant proteins was observed in MCF-7 cells. Soluble recombinant PTS1 and LLO-PTS1 proteins were produced in SHuffle T7 strain of E. coli with high efficiency of disulfide bonds formation. Conclusion:The LLO-PTS1 with corrected disulfide bonds was successfully expressed in E. coli SHuffle T7 strain. Due to the safety for human cells, this chimeric molecule can be an option to prevent pertussis disease if its immunostimulatory effects would be confirmed in the future. Key Words: Adjuvant, Cloning, Fusion protein, Pertussis toxin     

Mitigating effects of slash on soil disturbance in ground-based skidding operations

Reinforcement of skid trails with slash has been shown to reduce soil disturbances, but there have not been any studies documenting the mitigating effects following traffic of harvest equipment traffic on steep mountainous skid trails. The objective of this study was to quantify potentially mitigating effects of slash cover on soil compaction and rutting on skid trails in mountainous hardwood forests. The effects of the placement of no slash (bare soil), light density slash (7.5 kg m^?2) and heavy density slash (17.5 kg m^?2) in a skid trail following one, five, and nine machine passes on both gentle slopes <20% and steep slopes >20% in a downhill skidding operation by a steel-tracked skidder were studied. Bulk density and rut depth increased following harvest equipment traffic on both slope gradients. Compared to bare soils, soil bulk density was not significantly reduced by light slash density; however, soil bulk density was significantly reduced by heavy slash up to five machine passes on steep slopes. Light and heavy slash significantly reduced rut depth in both slope classes. The study revealed a high protective role of slash, particularly on steep skid trails. However, benefits of slash to mitigate soil compaction were limited to five passes, after which the slash deteriorated and only provided benefits against rutting.     

Genetic correlations between growth and reproductive traits in Zandi sheep

For the first time, the current study reports the genetic and phenotypic correlations between growth and reproductive traits in Zandi sheep. The data were comprised of 4,309 records of lamb growth traits from 1,378 dams and 273 sires plus 2,588 records of reproductive traits from 577 ewes. These data were extracted from available performance records at Khojir Breeding Station of Zandi sheep in Tehran, Iran, from 1993 to 2008. Correlations were estimated from two animal models in a bivariate analysis using restricted maximum likelihood procedure between lamb growth traits [birth weight (BW), weaning weight at 3 months of age (WW), as well as six-month weight (6 MW)] and ewe reproductive traits [litter size at birth (LSB), litter size at weaning (LSW), total litter weight at birth (TLWB), and total litter weight at weaning (TLWW)]. The genetic correlations between BW and reproductive traits varied from low to high ranges from 0.10 for BW–LSB to 0.86 for BW–TLWB. WW was moderately (0.37) to highly (0.96) correlated with all the reproductive traits. Moreover, the genetic correlations were observed between 6 MW and reproductive traits, varied from 0.19 to 0.95. Relationships between growth and reproductive traits ranged from 0.01 for BW–LSW to 0.28 for BW–TLWB in phenotypic effects. Results indicated that selection to improve WW would have high effect on genetic response in TLWW, and also, these results could be effective for all of the reproductive traits in Zandi sheep.     

Incidence of Fusarium verticillioides and levels of fumonisins in corn from main production areas in Iran

A total of 52 corn samples collected in 2000 from four main corn production provinces of Iran (Fars, Kermanshah, Khuzestan, and Mazandaran) were analyzed for contamination with Fusarium verticillioides and fumonisins (FB(1), FB(2), FB(3), and 3-epi-FB(3)). The mean incidence of F. verticillioides (percent of kernels infected) for these four areas was 26.7, 21.4, 24.9, and 59.0%, respectively. The incidence in Mazandaran was significantly (p < 0.05) above that of the other areas. All samples from Mazandaran were contaminated with fumonisins with a mean level of total fumonisins of 10674 microg/kg. In contrast, the incidence of fumonisin contamination above 10 microg/kg was 53 (8/15), 42 (5/12), and 57% (8/14) in the samples from Fars, Kermanshah, and Khuzestan, respectively, and the corresponding mean total fumonisin levels were 215, 71, and 174 microg/kg, respectively. No statistical differences (p > 0.05) were observed in the fumonisin levels of the corn samples from these three provinces, which were significantly (p < 0.05) lower than the fumonisin contamination in samples from Mazandaran.     

Prestorage heat treatment to maintain nutritive and functional properties during postharvest cold storage of pomegranate

Heat treatments have been used to extend storability of several fruits, although no information is available about their effects on nutritive and functional properties in pomegranates, which was the objective of this research. Thus, pomegranate fruits were heat treated (dips at 45 degrees C for 4 min) and stored at 2 degrees C for 90 days. Every 15 days, samples were taken and further stored 2 days at 20 degrees C for shelf life study. Arils from heat-treated pomegranates exhibited higher total antioxidant activity than controls, which was correlated primarily to the high levels of total phenolics and to lesser extent to ascorbic acid and anthocyanin contents. Additionally, the levels of sugars (glucose and fructose) and organic acids (malic, citric, and oxalic acids) remained also at higher concentrations in arils from treated fruits. With this simple and non-contaminant technology, the functional and nutritive properties, after long periods of storage, could then be even greater than in recently harvested fruits, thus providing a high content in health-beneficial compounds to consumers after the intake of these fruits.     

Identifying invasive fish species threats to RAMSAR wetland sites in the Caspian Sea region—A case study of the Anzali Wetland Complex (Iran)

Risk screening tools play a crucial role in identifying potential high-risk non-native (NN) fish species. In this study, potentially invasive NN fish species in the Anzali Wetland Complex (AWC), which is located on the south coast of the Caspian Sea (Iran), were identified using the Aquatic Species Invasiveness Screening Kit (AS-ISK). Twenty-nine freshwater fish species were screened of which 13 exist in the AWC and 16 in close proximity to it (“horizon” species). Receiver operating characteristic curve analysis showed that AS-ISK could discriminate reliably between non-invasive and invasive fish species for the AWC. Mean threshold scores were 3.25 for the Basic Risk Assessment (BRA) and 11.75 for the BRA + CCA (BRA + Climate Change Assessment), and these, respectively, classified 89.7% and 86.2% of the species as high risk. The CCA resulted in an increase in the BRA scores for 86.2% of the species, suggesting the need to account in future NN species management for a likely increased invasiveness of those species under future climate conditions. These results suggest that AS-ISK could prove an effective tool for identifying potentially invasive NN freshwater fishes in other wetlands of the Caspian Sea basin.     

Changes of extracted bioactive compounds from brown algae (Cystoseira indica) after conversion to mill and tablet using a quantitative metabolomics approach

Aquaculture International - There is developing importance in the biomedical use of seaweeds such as brown algae, mostly due to their contents of bioactive substances. In the present study,...     

Irrigation effects on diameter growth of 2-year-old Paulownia tomentosa saplings

Species of Paulownia are fast-growing trees that are planted in many countries in green spaces and agroforestry systems. We studied the influence of irrigation on the diameter growth of 2-year-old Paulownia tomentosa saplings planted in 3 m 9 3 m grids at Nazlou Campus of Urmia University, Iran. Completely randomized experimental design was used with four irrigation amount, viz.10, 15, 20 and 25 L, and three irrigation intervals, viz. 3-and 6-days, in 8 replications. During the growth season, we measured collar diameters of all saplings at the end of each month. Diameter growth declined with reduced irrigation amount and increased irrigation interval. The lowest average diameter growth(9 mm) was recorded for 10 L water at 6-day intervals and maximum average diameter growth(28.7 mm) was recorded for 25 L volume at 3-day intervals. The latter treatment resulted in significantly greater diameter growth than any other treatment(except20 L at 3-day intervals). More than 80 % of diameter growth was achieved in the first 3 months. Therefore, it is essential to irrigate with 25 L of water at 3-day intervals during the first three growth months to achieve maximum diameter growth for 2-year-old P. tomentosa saplings.     

Downed logs improve soil properties in old-growth temperate forests of northern Iran

Dead trees, particularly downed logs, play an important role in the dynamics of forest ecosystem. Contribution of decaying wood to C and nutrient pools of forest soils depends on the tree species and degree of wood decay. However, the extent to which the downed logs affect the soil properties of temperate forests has rarely been evaluated. In this study, a mixed beech forest was selected in Liresar region of Mazandaran Province, northern Iran, to investigate if and how the presence of downed logs affected soil quality and function by comparing soils underneath degraded logs and nearby soils of the two dominant tree species(beech and hornbeam). We then explored how these effects occurred as downed logs decomposed by comparing the woods of both tree species at four degrees of decomposition. Degree of decay of downed logs was classified into four classes(DC1–DC4). Eight dead trees of each tree species were selected at the center of each sample plot. Three composite soil samples underneath each decaying log and 100 cm away from a decaying log were collected at two soil depths(0–15 and 15–30 cm) to analyze soil main physicochemical properties and microbial activity. The results revealed that downed logs affected soil physical(5% wetter than control soils), chemical(2% lower pH, 100% increase in organic C and total N in the case of hornbeam, and 2% increase in P), and biological characteristics(soil microbial respiration enhanced by 10%, and microbial biomass C 620 and 351.5 mg kg~(-1) and microbial biomass N 66.47 and 32.18 mg kg~(-1), respectively, in the cases of beech and hornbeam), thus resulting in significantly different soil microsites from those without downed logs. Presence of downed logs increased soil microbial activity and soil fertility as wood decayed. Thus, the presence of downed logs is an important factor influencing forest soils and should be taken into consideration in forest management practices.