全文获取类型
收费全文 | 954篇 |
免费 | 67篇 |
国内免费 | 1篇 |
专业分类
林业 | 67篇 |
农学 | 49篇 |
基础科学 | 4篇 |
281篇 | |
综合类 | 69篇 |
农作物 | 83篇 |
水产渔业 | 108篇 |
畜牧兽医 | 266篇 |
园艺 | 23篇 |
植物保护 | 72篇 |
出版年
2024年 | 2篇 |
2023年 | 11篇 |
2022年 | 27篇 |
2021年 | 33篇 |
2020年 | 39篇 |
2019年 | 25篇 |
2018年 | 50篇 |
2017年 | 45篇 |
2016年 | 42篇 |
2015年 | 35篇 |
2014年 | 53篇 |
2013年 | 55篇 |
2012年 | 62篇 |
2011年 | 82篇 |
2010年 | 40篇 |
2009年 | 46篇 |
2008年 | 67篇 |
2007年 | 55篇 |
2006年 | 52篇 |
2005年 | 46篇 |
2004年 | 27篇 |
2003年 | 48篇 |
2002年 | 32篇 |
2001年 | 6篇 |
2000年 | 6篇 |
1999年 | 7篇 |
1998年 | 7篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1985年 | 2篇 |
1983年 | 2篇 |
1981年 | 1篇 |
排序方式: 共有1022条查询结果,搜索用时 15 毫秒
31.
Variations in soil chemical properties and microbial biomass in artificial gaps in silver fir stands
Changes in soil chemical and microbiological parameters in small (185 m2) and medium (410 m2) gaps, created in mature silver fir (Abies alba Mill) stands (mean height 30 m) in the Calabrian Apennines (Southern Italy), were studied over 2 years. Medium gaps had high
soil temperature and photosynthetically active radiation (PAR) transmittance and low soil moisture. Within small gaps we detected
a greater amount of organic matter, with respect to under canopy cover sites and medium gaps. Moreover, a different trend
of the organic matter between small and medium gaps was observed. In the medium gaps, with respect to under canopy cover sites
and small gaps, we found a relatively low content of organic matter, associated to a lower amount of humic acid, which suggested
that the organic substrate undergoes a mineralization rather than a humification process. An opposite trend was observed in
small gaps, where the increase of organic matter content, associated to an increase of humic acid and microbial biomass, suggested
a better humification process. Moreover, in small gaps the highest level of urease and the greatest fluorescein diacetate
(FDA) hydrolysis were observed. The different trend of organic matter observed in small and medium gaps may be related to
changes in the environmental conditions. PAR transmittance, significantly higher in medium gaps, contributed to increase soil
temperature and decrease soil moisture, affecting soil microbial populations and organic matter trend. These results suggest
that the creation of small gaps represents the silvicultural practice with minor environmental impact. 相似文献
32.
33.
Rossi S Lesignoli F Germini A Faccini A Sforza S Corradini R Marchelli R 《Journal of agricultural and food chemistry》2007,55(7):2509-2516
PCR products obtained by selective amplification of transgenic DNA derived from food samples containing Roundup Ready soybean or Bt-176 maize have been analyzed by anion-exchange HPLC. Peptide nucleic acids (PNAs), oligonucleotide analogues known to bind to complementary single-stranded DNA with high affinity and specificity, have been used as specific probes in order to assess the identity of the peaks observed. Two different protocols were adopted in order to obtain single-stranded DNA: amplification with an excess of one primer or digestion of one DNA strand. The single-stranded DNA was mixed with the PNA probe, and the presence of a specific sequence was revealed through detection of the corresponding PNA:DNA peak with significantly different retention time. Advantages and limits of this approach are discussed. The method was tested with reference materials and subsequently applied to commercial samples. 相似文献
34.
Irrigation effects on quality, phenolic composition, and selected volatiles of virgin olive oils cv. Leccino 总被引:1,自引:0,他引:1
Servili M Esposto S Lodolini E Selvaggini R Taticchi A Urbani S Montedoro G Serravalle M Gucci R 《Journal of agricultural and food chemistry》2007,55(16):6609-6618
Field-grown olive trees (Olea europaea L. cv. Leccino) were used over two growing seasons to determine the effect of deficit irrigation regimes on virgin olive oil (VOO) quality. Drip irrigation was managed to maintain a predawn leaf water potential (PLWP): (a) higher than -1.1 MPa (full irrigation: FI); (b) between -1.0 and -3.3 MPa (deficit irrigation: DI); (c) higher than -4.2 MPa (severe deficit irrigation: SI). The fruit yield and oil yield of DI trees were over 90% of those of FI treatments in both years, respectively, whereas yields of SI trees ranged from 61 to 76%. The irrigation regime had minor effects on the free acidity, peroxide value, and fatty acid composition of VOO. The concentrations of phenols and o-diphenols in VOO were negatively correlated with PLWP. The concentrations of the dialdehydic form of decarboxymethyl elenolic acid linked to (3,4-dihydroxyphenyl)ethanol (3,4-DHPEA-EDA), the isomer of the oleuropein aglycon (3,4-DHPEA-EA), and the dialdehydic form of decarboxymethyl elenolic acid linked to (p-hydroxyphenyl)ethanol (p-HPEA-EDA) were lower in FI than in SI treatments. The concentrations of lignans (+)-1-acetoxipinoresinol and (+)-1-pinoresinol were unaffected by the irrigation regime. The tree water status had a marked effect on the concentration of volatile compounds, such as the C(6)-saturated and unsaturated aldehydes, alcohols, and esters. 相似文献
35.
Primo Anacláudia Alves Araújo Maria Diana Melo Silva Karla da Fonseca Silva Ludmyla Araújo Pereira Graziella de Andrade Carvalho Fernandes Francisco Éden Paiva Pompeu Roberto Cláudio Fernandes Franco Natale William de Souza Henrique Antunes 《Agroforestry Systems》2021,95(8):1459-1464
Agroforestry Systems - The use of native trees in agroecosystems is a promising way to increase litter deposition and nutrient cycling and foster the recovery of degraded areas, especially in... 相似文献
36.
Ken Steen Pedersen Markku Johansen ?ystein Angen Sven Erik Jorsal Jens Peter Nielsen Tim K Jensen Roberto Guedes Marie St?hl Poul B?kbo 《Irish veterinary journal》2014,67(1)
Background
The major indication for antibiotic use in Danish pigs is treatment of intestinal diseases post weaning. Clinical decisions on antibiotic batch medication are often based on inspection of diarrhoeic pools on the pen floor. In some of these treated diarrhoea outbreaks, intestinal pathogens can only be demonstrated in a small number of pigs within the treated group (low pathogen diarrhoea). Termination of antibiotic batch medication in herds suffering from such diarrhoea could potentially reduce the consumption of antibiotics in the pig industry. The objective of the present pilot study was to suggest criteria for herd diagnosis of low pathogen diarrhoea in growing pigs.Data previously collected from 20 Danish herds were used to create a case series of clinical diarrhoea outbreaks normally subjected to antibiotic treatment. In the present study, these diarrhoea outbreaks were classified as low pathogen (<15% of the pigs having bacterial intestinal disease) (n =5 outbreaks) or high pathogen (≥15% of the pigs having bacterial intestinal disease) (n =15 outbreaks). Based on the case series, different diagnostic procedures were explored, and criteria for herd diagnosis of low pathogen diarrhoea were suggested. The effect of sampling variation was explored by simulation.Results
The diagnostic procedure with the highest combined herd-level sensitivity and specificity was qPCR testing of a pooled sample containing 20 randomly selected faecal samples. The criteria for a positive test result (high pathogen diarrhoea outbreak) were an average of 1.5 diarrhoeic faecal pools on the floor of each pen in the room under investigation and a pathogenic bacterial load ≥35,000 per gram in the faecal pool tested by qPCR. The bacterial load was the sum of Lawsonia intracellularis, Brachyspira pilosicoli and Escherichia coli F4 and F18 bacteria per gram faeces. The herd-diagnostic performance was (herd-level) diagnostic sensitivity =0.99, diagnostic specificity =0.80, positive predictive value =0.94 and negative predictive value =0.96.Conclusions
The pilot study suggests criteria for herd diagnosis of low pathogen diarrhoea in growing pigs. The suggested criteria should now be evaluated, and the effect of terminating antibiotic batch medication in herds identified as suffering from low pathogen diarrhoea should be explored.Electronic supplementary material
The online version of this article (doi:10.1186/2046-0481-67-24) contains supplementary material, which is available to authorized users. 相似文献37.
Manuel F. Chamorro Paul H. Walz Deborah M. Haines Thomas Passler Thomas Earleywine Roberto A. Palomares Kay P. Riddell Patricia Galik Yijing Zhang M. Daniel Givens 《Canadian journal of veterinary research》2014,78(2):81-88
Colostrum-replacement products are an alternative to provide passive immunity to neonatal calves; however, their ability to provide adequate levels of antibodies recognizing respiratory viruses has not been described. The objective of this study was to compare the serum levels of IgG at 2 d of age and the duration of detection of antibodies to bovine viral diarrhea virus 1 (BVDV-1), bovine viral diarrhea virus 2 (BVDV-2), bovine respiratory syncytial virus (BRSV), bovine herpesvirus 1 (BHV-1), and bovine parainfluenza virus 3 (BPIV-3) in calves fed maternal colostrum (MC) or a colostrum replacement (CR) at birth. Forty newborn male Holstein calves were assigned to the CR or the MC group. Group CR (n = 20) received 2 packets of colostrum replacement (100 g of IgG per 470-g packet), while group MC (n = 20) received 3.8 L of maternal colostrum. Blood samples for detection of IgG and virus antibodies were collected from each calf at birth, at 2 and 7 d, and monthly until the calves became seronegative. Calves in the MC group had greater IgG concentrations at 2 d of age. The apparent efficiency of absorption of IgG was greater in the MC group than in the CR group, although the difference was not significant. Calves in the CR group had greater concentrations of BVDV neutralizing antibodies during the first 4 mo of life. The levels of antibodies to BRSV, BHV-1, and BPIV-3 were similar in the 2 groups. The mean time to seronegativity was similar for each virus in the 2 groups; however, greater variation was observed in the antibody levels and in the duration of detection of immunity in the MC group than in the CR group. Thus, the CR product provided calves with more uniform levels and duration of antibodies to common bovine respiratory viruses. 相似文献
38.
Barley (Hordeum vulgare L.) grain germination is required to perform the malting process. Maintenance of barley seed viability during storage is crucial for the malt industry; and modern cultivars are bred for rapid grain dormancy release after physiological maturity. Low dormancy level combined with rain close to harvest induces pre-germination/pre-harvest sprouting damage. Pre-germination might not affect viability in the short term after harvest, but it could reduce potential longevity (Ki) of a barley seed lot. Ki value is inherent for each barley lot; however, its determination is time-consuming which precludes its assessment at an industrial scale. In this study we sought quantitative relationships between Ki and the pre-germination degree of barley grain lots, assessed through quality tests routinely performed by malthouses [Falling Number (FN), α-Amylase Activity and Carlsberg]. Field pre-germinated lots from one old barley cultivar (Quilmes Palomar) and artificially pre-germinated lots from major varieties currently grown in Argentina were used. Associations between Ki and values obtained from all quality tests analysed were found for Q. Palomar. However, FN was the parameter that yielded the best and simplest explanation of Ki variability. A significant positive linear Ki -FN relationship was also obtained for each modern barley cultivar. 相似文献
39.
Cesar Ricardo Arruda Fernanda Ramiro Vanessa Faria Roberto Barcelos Daniel Pontes Fernanda Passos Fabiano Kaiser Karoline dos Santos Teixeira Aline Maria Serrano Aline Abreu Leticia Siqueira Danielle Teixeira Matheus Vezzone Mariana Polivanov Helena Castilhos Zuleica 《Journal of Soils and Sediments》2022,22(2):547-558
Journal of Soils and Sediments - This study consists of the evaluation of metals acute toxicity levels taking into consideration a hypothetic scenario of dam breaking and land deposition of two... 相似文献
40.
Mainente Federica Piovan Anna Zanoni Francesca Chignola Roberto Cerantola Silvia Faggin Sofia Giron Maria Cecilia Filippini Raffaella Seraglia Roberta Zoccatelli Gianni 《Plant foods for human nutrition (Dordrecht, Netherlands)》2022,77(3):467-473
Plant Foods for Human Nutrition - Silver linden (Tilia tomentosa Moench, TtM) flowers possess several health-promoting properties, especially at the neurological level, such as intestinal... 相似文献