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81.
Dose response curves: their use in soil microbiological research and possibilities of evaluation The relation between different concentrations of a toxicant in soils and the resulting effects on biological parameters can be described with dose response curves. Dose response curves make it possible to determine the concentrations of a chemical that create definite effects (?Effective Doses”?, e.g. ED10 and ED50 for 10- and 50% inhibitory effects) or to evaluate the rate of increase of inhibition with increasing concentrations. Some of the mostly used ways to extract these parameters from dose response curves are presented and examined with regard to their suitability in soil microbiological research. Besides graphical procedures for the evaluation of dose response curves a mathematical model for fitting quantitative dose response curves is introduced. With this model the confidence intervals of effective doses can be computed. By showing eight frequently appearing shapes of dose response curves the advantages and limitations of different ways to calculate effective doses are discussed. 相似文献
82.
Krist S Stuebiger G Unterweger H Bandion F Buchbauer G 《Journal of agricultural and food chemistry》2005,53(21):8310-8316
Poppy seed oil (Oleum Papaveris Seminis) is used for culinary and pharmaceutical purposes, as well as for making soaps, paints, and varnishes. Astonishingly, hardly anything was yet known about the volatile compounds of this promising comestible. Likewise, there are no current published data about the triglyceride (TAG) composition of poppy seed oils available. In this investigation solid-phase microextraction (SPME) with DVB/Carboxen/PDMS Stable-Flex fiber was applied to the study of volatile compounds of several seed oil samples from Papaver somniferum L. (Papaveraceae). 1-Pentanol (3.3-4.9%), 1-hexanal (10.9-30.9%), 1-hexanol (5.3-33.7%), 2-pentylfuran (7.2-10.0%), and caproic acid (2.9-11.5%) could be identified as the main volatile compounds in all examined poppy seed oil samples. Furthermore, the TAG composition of these oils was analyzed by MALDI-ReTOF- and ESI-IT-MS/MS. The predominant TAG components were found to be composed of linoleic, oleic, and palmitic acid, comprising approximately 70% of the oils. TAG patterns of the different poppy varieties were found to be very homogeneous, showing also no significant differences in terms of the applied pressing method of the plant seeds. 相似文献
83.
Schwarz M Quast P von Baer D Winterhalter P 《Journal of agricultural and food chemistry》2003,51(21):6261-6267
Vitisin A was prepared from malvidin 3-glucoside and pyruvic acid in model wine medium, isolated by countercurrent chromatography, and purified by preparative high-performance liquid chromatography (HPLC). The synthesized compound was used as a reference standard to quantify vitisin A in Chilean wines from Vitis vinifera cv. Cabernet Sauvignon, including a vertical row of wines from the same vineyard over 16 years. Maximum vitisin A content was reached within the first year of storage. Importantly, up to half of the initial amount of vitisin A in young wines was still present in 15 year old wines. Although vitisin A was found to be much more stable as compared to other monomeric C-4 underivatized anthocyanins, it also slowly degrades after reaching its peak concentration. The "color activity concept" was applied to vitisin A, malvidin 3-glucoside, malvidin 3-(6' '-acetylglucoside), and polymeric pigments isolated by countercurrent chromatography in order to estimate their contribution toward the overall color expression of wines. It was found that vitisin A is only a minor contributor to the visually perceived color of aged red wines (color contribution approximately 5%). The major contributor is the polymeric fraction (color contribution approximately 70-90%). 相似文献
84.
Seedlings of alfalfa, rape, spinach, and wheat, potted on sandy soil, were irrigated with an aqueous extract of pea shoot (PE, 9.84 g dry weight l–1) or a solution of Ca, K, Mg, P, and NO3‐N salts (SS) in a concentration similar to that in PE, for 31–48 days. In comparison to water‐irrigated controls, both SS and PE treated plants showed nearly equal increases in shoot dry weight (29–40 %), whereas PE‐treated plants had higher fresh weights (38–84 %) due to increased succulence. Treatment with SS did not enhance, but sometimes even reduce, the concentrations of Ca, K, Mg, and several trace elements in shoot tissues. In contrast, PE‐treated plants had higher Ca, K, Mg, and organic N, but lower As and Ni contents and were thus of higher nutritive value. Reduced contents of metals in plant tissue correlated with their reduced solubility in the soil solution, which was not due to changes in pH. Fertilizer components such as K and Mg (metals of lower exchange intensity) were incorporated into the soil to release Ca, Sr, and Ba (metals of higher exchange intensity) and reduce the solubility of most trace elements and metal‐complexing humic substances. In addition, application of Ca precipitated heavy metals and humic complexes directly from the soil solution. This effect was partially overcome by PE. Its carboxylic acids could act as phytochelators of metal ions and as mobilizers of the highly diffusible humic substances which carry metals to roots. It is concluded that continuous PE application replaces the quantities of Ca, K, Mg, P, and organic N, but not of NO3‐N consumed during plant growth. Using PE does not add any relevant quantities of toxic metals to the plant‐soil system. 相似文献
85.
A nuimber of compounds with terpenoid skeletons attached to various functional groups were prepared and tested for their effects as a juvenile hormone in the yellow mealworm Tenebrio molitor. Several of these compounds showed high activity. 相似文献
86.
Busche MA Eichhoff G Adelsberger H Abramowski D Wiederhold KH Haass C Staufenbiel M Konnerth A Garaschuk O 《Science (New York, N.Y.)》2008,321(5896):1686-1689
The neurodegeneration observed in Alzheimer's disease has been associated with synaptic dismantling and progressive decrease in neuronal activity. We tested this hypothesis in vivo by using two-photon Ca2+ imaging in a mouse model of Alzheimer's disease. Although a decrease in neuronal activity was seen in 29% of layer 2/3 cortical neurons, 21% of neurons displayed an unexpected increase in the frequency of spontaneous Ca2+ transients. These "hyperactive" neurons were found exclusively near the plaques of amyloid beta-depositing mice. The hyperactivity appeared to be due to a relative decrease in synaptic inhibition. Thus, we suggest that a redistribution of synaptic drive between silent and hyperactive neurons, rather than an overall decrease in synaptic activity, provides a mechanism for the disturbed cortical function in Alzheimer's disease. 相似文献
87.
EFFECTS OF FLUID AND COMPUTED TOMOGRAPHIC TECHNICAL FACTORS ON CONSPICUITY OF CANINE AND FELINE NASAL TURBINATES 下载免费PDF全文
Raimonda Uosyte Darren J. Shaw Danielle A. Gunn‐Moore Eduardo Fraga‐Manteiga Tobias Schwarz 《Veterinary radiology & ultrasound》2015,56(5):494-502
Turbinate destruction is an important diagnostic criterion in canine and feline nasal computed tomography (CT). However decreased turbinate visibility may also be caused by technical CT settings and nasal fluid. The purpose of this experimental, crossover study was to determine whether fluid reduces conspicuity of canine and feline nasal turbinates in CT and if so, whether CT settings can maximize conspicuity. Three canine and three feline cadaver heads were used. Nasal slabs were CT‐scanned before and after submerging them in a water bath; using sequential, helical, and ultrahigh resolution modes; with images in low, medium, and high frequency image reconstruction kernels; and with application of additional posterior fossa optimization and high contrast enhancing filters. Visible turbinate length was measured by a single observer using manual tracing. Nasal density heterogeneity was measured using the standard deviation (SD) of mean nasal density from a region of interest in each nasal cavity. Linear mixed‐effect models using the R package ‘nlme’, multivariable models and standard post hoc Tukey pair‐wise comparisons were performed to investigate the effect of several variables (nasal content, scanning mode, image reconstruction kernel, application of post reconstruction filters) on measured visible total turbinate length and SD of mean nasal density. All canine and feline water‐filled nasal slabs showed significantly decreased visibility of nasal turbinates (P < 0.001). High frequency kernels provided the best turbinate visibility and highest SD of aerated nasal slabs, whereas medium frequency kernels were optimal for water‐filled nasal slabs. Scanning mode and filter application had no effect on turbinate visibility. 相似文献
88.
A proposal of clinical breakpoints for amoxicillin applicable to porcine respiratory tract pathogens
Schwarz S Böttner A Goossens L Goosens L Hafez HM Hartmann K Kaske M Kehrenberg C Kietzmann M Klarmann D Klein G Krabisch P Luhofer G Richter A Schulz B Sigge C Waldmann KH Wallmann J Werckenthin C 《Veterinary microbiology》2008,126(1-3):178-188
In the present position paper, an attempt was made to establish clinical breakpoints of amoxicillin to classify porcine respiratory tract pathogens as susceptible, intermediate or resistant based on their minimum inhibitory concentrations of amoxicillin. For this, a thorough review of the published literature with regard to swine-specific pharmacological data (including dosages of amoxicillin applied and routes of administration used), clinical efficacy, and in vitro susceptibility of the target pathogens was performed. Based on the comparative analysis of the results, the working group "Antibiotic Resistance" of the German Veterinary Medical Society (DVG) proposed to classify porcine respiratory tract pathogens that show MIC values of amoxicillin of < or =0.5microg/ml as "susceptible", those with MICs of 1microg/ml as "intermediate", and those with MICs of > or =2microg/ml as "resistant". 相似文献
89.
Hampel V Schwarz B Kempf C Köstlin R Schillinger U Küchenhoff H Fenske N Brill T Hirschberger J 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(6):1340-1346
BACKGROUND: Recombinant feline interferon-omega (rFeIFN-omega) was tested as a treatment option for cats with fibrosarcoma to assess safety and feasibility. HYPOTHESIS: Treatment with rFeIFN-omega in cats with fibrosarcoma is safe and feasible. ANIMALS: Twenty domestic cats. METHODS: In an open-labeled uncontrolled clinical trial 12 injections of 1 x 10(6) U/kg rFeIFN-omega were administered over a 5-week period: the 1st through 4th injections were given intratumorally, and the 5th through 12th injections were administered subcutaneously at the tumor excision site. Wide surgical excision of the tumors was carried out after the 4th injection and before the 5th injection of rFeIFN-omega. A Common Terminology Criteria for Adverse Events (CTCAE) analysis was conducted. Flow cytometry of fibrosarcoma cells after incubation with rFeIFN-omega and recombinant feline interferon-gamma was performed to assess the biological effect of rFeIFN-omega. RESULTS: Changes in blood cell count, increases in serum aspartate-amino-transferase activity, serum bilirubin concentration, serum creatinine and serum electrolyte concentrations, weight loss, anorexia, increased body temperature, and reduced general condition were observed but were mostly minor (grade 1 and 2) and self limiting. Eosinophilia (P = .025), neutropenia (P = .021), and weight loss (P < .001) were statistically correlated with rFeIFN-omega-treatment (analysis of parameters before treatment and after 3 injections of rFeIFN-omega). Flow cytometry of 5 unrelated feline fibrosarcoma cell lines showed increased expression of major histocompatibility complex (MHC) class I molecules (P = .026) in response to in vitro incubation with rFeIFN-omega, whereas expression of MHC class II molecules was not affected significantly. CONCLUSIONS AND CLINICAL IMPORTANCE: RFeIFN-omega for the treatment of feline fibrosarcoma is safe, well tolerated, and can be easily performed in practice. To assess the efficacy of the treatment, it should be tested in a placebo-controlled trial. 相似文献
90.
Sutor A Schwarz S Conraths FJ 《Berliner und Münchener tier?rztliche Wochenschrift》2011,124(11-12):457-464
Some invasive wildlife species have the potential to act as additional host and vector species for parasitic and other infectious diseases. We used the raccoon dog (Nyctereutes procyonides), a carnivore species that has its origin in Asia, as an example to demonstrate biological and ecological prerequisites which enables an invasive species to occupy a new habitat permanently. Studies conducted during the last ten years identified a total of 23 endoparasites, two ectoparasites, six bacterial or protozoan species and five viruses, found in the Nyctereutes procyonoides ussuriensis subspecies in its newly occupied range or in N. procyonoides koreensis in its original range. Results of studies in Finland and Germany furthermore showed that biological characteristics of the raccoon dog make this carnivore an appropriate host or vector for a variety of parasites and infectious diseases. This may result in a growing importance of this invasive carnivore for the epidemiology of transmissible diseases in Germany. Especially with regard to zoonotic disease outbreaks, the raccoon dog should therefore be paid more attention in disease prevention and eradication strategies. 相似文献