全文获取类型
收费全文 | 1121篇 |
免费 | 54篇 |
国内免费 | 1篇 |
专业分类
林业 | 128篇 |
农学 | 24篇 |
基础科学 | 3篇 |
188篇 | |
综合类 | 133篇 |
农作物 | 44篇 |
水产渔业 | 57篇 |
畜牧兽医 | 533篇 |
园艺 | 11篇 |
植物保护 | 55篇 |
出版年
2021年 | 14篇 |
2020年 | 21篇 |
2019年 | 14篇 |
2018年 | 20篇 |
2017年 | 19篇 |
2016年 | 22篇 |
2015年 | 14篇 |
2014年 | 12篇 |
2013年 | 45篇 |
2012年 | 37篇 |
2011年 | 38篇 |
2010年 | 33篇 |
2009年 | 31篇 |
2008年 | 44篇 |
2007年 | 40篇 |
2006年 | 38篇 |
2005年 | 35篇 |
2004年 | 43篇 |
2003年 | 40篇 |
2002年 | 16篇 |
2001年 | 17篇 |
2000年 | 20篇 |
1999年 | 22篇 |
1998年 | 17篇 |
1997年 | 13篇 |
1996年 | 11篇 |
1995年 | 10篇 |
1994年 | 16篇 |
1993年 | 10篇 |
1992年 | 22篇 |
1991年 | 30篇 |
1990年 | 17篇 |
1989年 | 28篇 |
1988年 | 11篇 |
1987年 | 21篇 |
1986年 | 22篇 |
1985年 | 20篇 |
1984年 | 13篇 |
1982年 | 10篇 |
1979年 | 15篇 |
1978年 | 13篇 |
1976年 | 9篇 |
1974年 | 15篇 |
1973年 | 11篇 |
1972年 | 14篇 |
1970年 | 20篇 |
1969年 | 11篇 |
1968年 | 14篇 |
1967年 | 21篇 |
1966年 | 9篇 |
排序方式: 共有1176条查询结果,搜索用时 15 毫秒
11.
12.
Coetzee JF Schmidt PL Apley MD Reinbold JB Kocan KM 《American journal of veterinary research》2007,68(8):872-878
OBJECTIVE: To compare sensitivity of a complement fixation (CF) test and competitive ELISA (cELISA) for detection of Anaplasma marginale in experimentally infected steers. ANIMALS: 40 crossbred (Angus-Simmental) steers. PROCEDURES: Steers were inoculated with 2.6 x 10(9) A marginale-infected erythrocytes (day 0). Blood samples were collected on days 9, 13, 20, 28, 34, 41, 61, 96, 126, and 156 days after inoculation. The percentage of parasitized erythrocytes (PPE) was determined by microscopic examination of stained blood films, and sera were evaluated with the CF test and cELISA by use of USDA-approved methods. Sensitivity and agreement (kappa statistic) between the 2 methods were determined. Persistent infections were confirmed by inoculation of blood obtained from infected steers into susceptible, splenectomized calves. RESULTS: 9 days after inoculation, sensitivity of the cELISA was 47.5%, whereas the CF test failed to identify seropositive steers. After day 13, sensitivity of the cELISA and CF test was 100% and 20%, respectively. During peak parasitemia (day 20), sensitivity of the cELISA and CF test was 100%. Thereafter, sensitivity of the CF test fluctuated between 7.5% and 37.5%, whereas sensitivity of the cELISA remained at 100%. Overall sensitivity of the cELISA and CF test was 94.8% and 26.5%, respectively (kappa statistic, 0.039). CONCLUSIONS AND CLINICAL RELEVANCE: The cELISA had superior sensitivity for serologic detection of A marginale.The CF test and cELISA each had a high percentage of false-negative results during the prepatent period. These findings are relevant for export certification and anaplasmosis prevention or eradication programs. 相似文献
13.
Enterohemorrhagic Escherichia coli (EHEC) are a subgroup of Shiga toxin-producing Escherichia coli (STEC) that are able to cause serious food-borne intestinal diseases which can be followed in 5 to 15% by extraintestinal sequelae such as the hemolytic-uremic syndrome (HUS). One of the major pathogenicity factors of EHEC is the production of one or more Shiga toxins (Stx), which act as inhibitors of protein biosynthesis and have profound effects on the signal transduction and immunological response in eukaryotic cells. The stx genes are located in the genome of heterogeneous, lambdoid, functional or cryptic bacteriophages and are expressed during the phage life cycle. Due to the linkage between the phage life cycle and stx expression, STEC and their bacteriophages are useful as a model for the analysis of stress response and virulence of this food-borne pathogen. 相似文献
14.
15.
Zoledronate is a bisphosphonate frequently used for the treatment of hypercalcaemia of malignancy and tumour-associated bone pain in dogs, however, there is a paucity of information regarding its use in veterinary medicine. The aim of this retrospective study was to report the tolerability of zoledronate in the palliative treatment of cancer-bearing dogs and secondarily to to assess the efficacy of zoledronate for the treatment of hypercalcaemia of malignancy. Thirty-seven dogs (22 with tumour-associated bone pain and 15 with hypercalcaemia of malignancy) that received 114 zoledronate infusions were included. Tolerability was assessed by the absence of post-zoledronate hypocalcaemia or other adverse events as defined by Veterinary Cooperative Oncology Group-Common Terminology Criteria for Adverse Events criteria. Efficacy was assessed by comparison of available ionized calcium levels before and after zoledronate administration in hypercalcaemic dogs. In 79% of zoledronate infusions, no adverse events were reported. The majority of adverse events which occurred in the other 21% of infusions could be attributed to concurrent chemotherapy or the underlying neoplastic disease. There was a small but significant increase in creatinine following treatment with zoledronate, however, none of the dogs developed clinically significant renal disease. In eight hypercalcaemic dogs with available ionized calcium following zoledronate administration, ionized calcium decreased rapidly within 7 days following treatment with zoledronate. Zoledronate is well-tolerated with few recorded adverse events, however, monitoring of serum creatinine is advised. Zoledronate seems to be effective in the treatment of hypercalcaemia of malignancy. 相似文献
16.
Lasmar Chaim J. Queiroz Antônio C. M. Rosa Clarissa Carvalho Nathália S. Schmidt Fernando A. Solar Ricardo R. C. Paolucci Lucas N. Cuissi Rafael G. Ribas Carla R. 《Landscape Ecology》2021,36(9):2583-2597
Landscape Ecology - Harmful effects of habitat loss and fragmentation can be detected across multiple spatial scales, yet most studies that aim to characterize these effects take place at a single... 相似文献
17.
18.
19.
20.
Anna Karina R. Schmidt 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2017,67(5):416-424
Seed crops of white clover (Trifolium repens L.) are usually established with a cover crop. Provided sufficient light, white clover may compensate for low plant density by stoloniferous growth. Our objectives were (1) to compare spring barley or spring wheat used as cover crops for white clover and (2) to find the optimal seeding rate/row distance for white clover. Seven field trials were conducted in Southeast Norway from 2000 to 2003. Barley was seeded at 360 and 240 seeds m?2 and wheat at 525 and 350 seeds m?2. White clover was seeded perpendicularly to the cover crop at 400 seeds m?2/13 cm row distance or 200 seeds m?2/26 cm. Results showed that light penetration in spring and early summer was better in wheat than in barley. On average for seven trials, this resulted in 11% higher seed yield after establishment in wheat than in barley. The 33% reduction in cover crop seeding rate had no effect on white clover seed yield for any of the cover crops. Reducing the seeding rate/doubling the row distance of white clover had no effect on seed yield but resulted in slightly earlier maturation of the seed crop. 相似文献