全文获取类型
收费全文 | 739篇 |
免费 | 41篇 |
国内免费 | 1篇 |
专业分类
林业 | 42篇 |
农学 | 44篇 |
116篇 | |
综合类 | 48篇 |
农作物 | 107篇 |
水产渔业 | 56篇 |
畜牧兽医 | 333篇 |
园艺 | 9篇 |
植物保护 | 26篇 |
出版年
2023年 | 5篇 |
2022年 | 22篇 |
2021年 | 26篇 |
2020年 | 15篇 |
2019年 | 13篇 |
2018年 | 18篇 |
2017年 | 25篇 |
2016年 | 22篇 |
2015年 | 30篇 |
2014年 | 39篇 |
2013年 | 46篇 |
2012年 | 43篇 |
2011年 | 51篇 |
2010年 | 30篇 |
2009年 | 28篇 |
2008年 | 59篇 |
2007年 | 47篇 |
2006年 | 38篇 |
2005年 | 43篇 |
2004年 | 39篇 |
2003年 | 36篇 |
2002年 | 29篇 |
2001年 | 20篇 |
2000年 | 14篇 |
1999年 | 7篇 |
1998年 | 2篇 |
1997年 | 5篇 |
1996年 | 3篇 |
1993年 | 2篇 |
1992年 | 7篇 |
1991年 | 2篇 |
1990年 | 4篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1968年 | 1篇 |
排序方式: 共有781条查询结果,搜索用时 15 毫秒
41.
Weiwei Yan Kang-Soon Shin Shih-Jon Wang Hua Xiang Thomas Divers Sean McDonough James Bowman Anne Rowlands Bruce Akey Hussni Mohamed Yung-Fu Chang 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(2):249-258
Clostridium (C.) difficile is a common cause of nosocomial diarrhea in horses. Vancomycin and metronidazole have been used as standard treatments but are only moderately effective, which highlights the need for a novel alternative therapy. In the current study, we prepared antiserum of equine origin against both C. difficile toxins A and B as well as whole-cell bacteria. The toxin-neutralizing activities of the antibodies were evaluated in vitro and the prophylactic effects of in vivo passive immunotherapy were demonstrated using a conventional mouse model. The data demonstrated that immunized horses generated antibodies against both toxins A and B that possessed toxin-neutralizing activity. Additionally, mice treated with the antiserum lost less weight without any sign of illness and regained weight back to a normal range more rapidly compared to the control group when challenged orally with 107
C. difficile spores 1 day after serum injection. These results indicate that intravenous delivery of hyperimmune serum can protect animals from C. difficile challenge in a dose-dependent manner. Hence, immunotherapy may be a promising prophylactic strategy for preventing C. difficile infection in horses. 相似文献
42.
A new charge-transfer (CT) complex, consisting of 2,3-dichloro-5-nitro-1,4-naphthoquinone and biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) has been designed to sensing biothiols. The method is selective and sensitive for biothiols detection without the interference of other amino acids. 相似文献
43.
44.
Han SY Kang BK Kang BJ Shin SP Soen BH Kim JM Kim JH Choresca CH Han JE Jun JW Park SC 《Journal of fish diseases》2011,34(10):731-739
The prevalence of two serotypes of Streptococcus parauberis isolated from the olive flounder, Paralichthys olivaceus, was evaluated in a total of 29 isolates between 2003 and 2010 in Korea. Streptococcus parauberis isolates were divided into two serologically distinct types (serotype 1 and serotype 2), except for one strain (S1091), using an agglutination assay with rabbit antiserum, and serotype 1 was identified as the dominant type (24 of 29 isolates) in this study. To identify the characteristics of the two serotypes of S. parauberis, we conducted a biochemical test using the API 20 Strep kit, a transmission electron microscopy (TEM) assay, sequence analysis of 16S‐23S rRNA intergenic spacer region (ISR) and a pathogenicity test. In TEM, both serotypes possessed polysaccharide capsule layers around the cell surface when bacterial cells were treated with a homologous serotype of rabbit antiserum. However, we were unable to discriminate serotype‐specific biochemical characteristics and genetic characteristics of 16S‐23S rRNA ISR between the two serotypes. In the pathogenicity test, the serotype 1 strains induced significantly higher mortality than the serotype 2 strains in olive flounder when experimentally inoculated via the intraperitoneal route. 相似文献
45.
46.
The immune receptor-mediated functions of bovine alveolar macrophages (AM) inoculated in vitro with bovine herpesvirus-1 (BHV-1) or parainfluenza-3 (PI-3) virus were tested in the presence or absence of virus-specific antiserum or pulmonary lavage fluids collected from calves 6 days after inoculation with BHV-1 or PI-3 virus. The Fc and C3b phagocytic indices of noninoculated AM, collected from 6- to 16-week-old calves, ranged from 75 to 87 and 59 to 64, respectively, and the binding indices ranged from 5 to 8 and 22 to 28, respectively. Infection of AM with either BHV-1 or PI-3 virus had no significant effect on receptor-mediated phagocytosis or binding, with the exception of a significant (P less than 0.05) decrease, from 64 to 46, of the C3b phagocytic index of PI-3 virus-infected AM. The addition of lavage fluids, collected after BHV-1 or PI-3 virus infection, to AM infected with the respective virus caused a significant (P less than 0.05) decrease in phagocytic indices with values for the Fc and C3b indices in BHV-1-infected AM decreasing from 81 to 49 and from 47 to 8, respectively, and those for the PI-3 virus-infected AM from 79 to 51 and from 46 to 15, respectively. The binding indices of virus-infected AM increased with the addition of viral lavage fluids, but the only significant (P less than 0.05) increase was for C3b binding in PI-3 virus-infected cells, which increased from 33 to 56.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
47.
48.
Chromosomes responsible for sensitivity of embryo to abscisic acid and dormancy in wheat 总被引:4,自引:0,他引:4
The sensitivity of the embryo to abscisic acid (ABA) has been reported toplay an important role in seed dormancy. Using ditelocentric lines of wheatcv. Chinese Spring (CS, nondormant and ABA insensitive), F2 seedsbetween monosomic lines of CS and a wheat line Kitakei-1354 (dormant,ABA sensitive) and deletion lines of CS chromosome 4A, germinability ofseeds and embryo-half seeds incubated in water and ABA were examined. The results indicated that the long arm of chromosome 4A carried majorgene(s) for the embryo sensitivity to ABA and dormancy. Chromosome2D might be also involved in the sensitivity to ABA. 相似文献
49.
Hoy-Taek Kim Yutaka Hirata Yong-Uk Shin Hae-Sung Hwang Jeong-Hwan Hwang Il-Sheob Shin Dae-Il Kim Sang-Jo Kang Hyun-Jung Kim Dong-Young Shin Ill-Sup Nou 《Euphytica》2004,138(1):73-80
The pear cultivar ‘Osa-Nijisseiki’ (S2Ssm
4; sm = stylar-part mutant) has been used as a parent to breed self-compatible cultivars that produce excellent fruits. However,
determination of the self-compatibility of ‘Osa-Nijisseiki’ offspring requires a lot of time, 6 years or more, by conventional
cross breeding. We have designed a rapid reliable method for the identification of self-compatible varieties of ‘Osa-Nijisseiki’
offspring based on the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) with S-allele specific
restriction endonucleases. By using this method, 8 self-compatible varieties were selected among 16 selections resulting from
a cross between the self-compatible cultivar ‘Osa-Nijisseiki’ (S2Ssm
4) and the self-incompatible cultivars ‘Niitaka’ (S3S9), ‘Whasan’ (S3S5), ‘Chuwhangbae’ (S4S6). The S-genotypes of 16 ‘Osa-Nijisseiki’ offsprings were also determined.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
50.
Various pigment colors were produced by Monascus fermentations with separate addition of 20 amino acids. The color characteristics and structures of the pigment derivatives were investigated. When each amino acid was added to the fermentation broth as a precursor, pigment extracts with different hue and chroma values were obtained depending on the content ratios of yellow, orange, and red colors in the fermentation broth. The yellow and orange pigments were identical regardless of amino acid addition. The red compounds varied on the basis of the type of amino acid added. LC-MS and (1)H and (13)C NMR structural analyses confirmed that the derivative pigments contained the moieties of the added amino acids. L, a, and b values of the CIELAB color system for the derivative pigments were measured. Values of hue and chroma were then calculated. The colors of the derivative pigments were in the range of orangish red to violet red. The hydrophilicities/hydrophobicities of the derivative pigments could be predicted from their log P values, which were estimated using computer programs. 相似文献