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171.
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Sabine C. Riesen Karsten E. Schober Rebecca M. Cervenec John D. Bonagura 《Journal of Veterinary Cardiology》2012,14(2):323-332
ObjectivesData on reproducibility of echocardiographic indices in cats are commonly derived from studies in healthy, non-treated animals. However, medical treatment may alter reproducibility of such data possibly influencing interpretation of results of clinical trials assessing the effects of drugs on cardiovascular function. The objectives were therefore to investigate the effects of ivabradine and atenolol on reproducibility of echocardiographic indices of left heart function.AnimalsEight healthy cats.MethodsRepeated echocardiographic examinations were performed by two observers in mildly sedated cats at baseline and after four weeks of treatment (Group 1, ivabradine 0.3 mg/kg q12 h PO, n = 4; Group 2, atenolol 6.25 mg/cat q12 h PO, n = 4) in a prospective, double-blind, randomized study. Test reliability was determined by estimating measurement variability, within-day interobserver variability, and between-day intraobserver variability of all echocardiographic indices. Variability was expressed as coefficient of variation (CV) and the absolute value below which the difference between two measurements lay with 95% probability. Effects of treatments on variability were compared using linear mixed effects models ANOVA and Fisher's exact test.ResultsOverall, CVs ranged from 0.5 to 50.6% at baseline, 0.5–45.5% after ivabradine, and 0.5–23.3% after atenolol. Reproducibility of all variables determined did neither improve nor worsen consistently after either treatment although atenolol exhibited a tendency toward higher reliability with none of the CVs exceeding 24% as compared to ivabradine.ConclusionsTreatment of healthy cats with either atenolol or ivabradine had only minor effects on reproducibility of echocardiographic data. Whether these findings can be extrapolated to cats with hypertrophic cardiomyopathy deserves further study. 相似文献
173.
Response of soil microbial biomass and enzyme activities to the transient elevation of carbon dioxide in a semi-arid grassland 总被引:1,自引:0,他引:1
Ellen Kandeler Arvin R. Mosier Daniel G. Milchunas Sabine Rudolph 《Soil biology & biochemistry》2006,38(8):2448-2460
Although elevation of CO2 has been reported to impact soil microbial functions, little information is available on the spatial and temporal variation of this effect. The objective of this study was to determine the microbial response in a northern Colorado shortgrass steppe to a 5-year elevation of atmospheric CO2 as well as the reversibility of the microbial response during a period of several months after shutting off the CO2 amendment. The experiment was comprised of nine experimental plots: three chambered plots maintained at ambient CO2 levels of 360 μmol mol−1 (ambient treatment), three chambered plots maintained at 720 μmol mol−1 CO2 (elevated treatment) and three unchambered plots of equal ground area used as controls to monitor the chamber effect.Elevated CO2 induced mainly an increase of enzyme activities (protease, xylanase, invertase, alkaline phosphatase, arylsulfatase) in the upper 5 cm of the soil and did not change microbial biomass in the soil profile. Since rhizodeposition and newly formed roots enlarged the pool of easily available substrates mainly in the upper soil layers, enzyme regulation (production and activity) rather than shifts in microbial abundance was the driving factor for higher enzyme activities in the upper soil. Repeated soil sampling during the third to fifth year of the experiment revealed an enhancement of enzyme activities which varied in the range of 20-80%. Discriminant analysis including all microbiological properties revealed that the enzyme pattern in 1999 and 2000 was dominated by the CO2 and chamber effect, while in 2001 the influence of elevated CO2 increased and the chamber effect decreased.Although microbial biomass did not show any response to elevated CO2 during the main experiment, a significant increase of soil microbial N was detected as a post-treatment effect probably due to lower nutrient (nitrogen) competition between microorganisms and plants in this N-limited ecosystem. Whereas most enzyme activities showed a significant post-CO2 effect in spring 2002 (following the conclusion of CO2 enrichment the previous autumn, 2001), selective depletion of substrates is speculated to be the cause for non-significant treatment effects of most enzyme activities later in summer and autumn, 2002. Therefore, additional belowground carbon input mainly entered the fast cycling carbon pool and contributed little to long-term carbon storage in the semi-arid grassland. 相似文献
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Iris Knaus Dr. Med. Vet. Sabine Breit Dr. Med. Vet. Wolfgang Künzel Dr. Med. Vet. Elisabeth Mayrhofer Dr. Med. Vet. 《Veterinary radiology & ultrasound》2004,45(1):1-9
Ventrodorsal (VD) radiographs of cadaveric pelves of large and giant breeds (n = 40) and of the corresponding bone specimens were made. Gross changes seen in the specimens were marked with metal wire or radiopaque paint to relate radiographic changes to the postmortem appearance of degenerative sacroiliac (SI) joint lesions. Two positioning techniques were used, resulting in two radiographs for each specimen. The techniques used were: (1) pulling the pelvic limbs caudally, with gentle traction producing a view of the sacrum in almost parallel alignment between the sacrum and the X-ray film (we have termed this a "central SI view") and (2) pulling the pelvic limbs caudally with greater traction than above, resulting in maximal inclination of the sacrum with respect to the X-ray film (we have termed this an "angled SI view"). Lesions of the supportive soft connective tissue could be imaged on the central and angled SI views, whereas angled SI views allowed better identification of lesions of the synovial SI joint. The insights gained in the above study were then used in a retrospective examination of 145 routine ventrodorsal radiographs of the pelvis (i.e., 290 sacroiliac joints) to determine the incidence of degenerative changes of the SI joint in large dogs. The lesions most commonly observed were calcification of the supportive soft connective tissue (n = 184/290), whereas lesions of the synovial SI joint were less frequent (n = 86/290). The degree of central or angled projection obtained in standard ventrodorsal radiographs was noted to be significantly (P < 0.05) associated with age, body conformation, anesthetic status, and the presence of spondylosis deformans at the lumbosacral junction. In contrast, SI joint disease and the gender of the animal did not affect the SI view achieved in VD radiographs. 相似文献
178.
Kästner SB Grundmann S Bettschart-Wolfensberger R 《Veterinary anaesthesia and analgesia》2004,31(3):227-230
A peri-carinal tracheal laceration was produced in a 11-year-old cat during tracheal intubation. Before reconstructive surgery began, the leak was bypassed with an endobronchial tube positioned using endoscopy and direct vision. However, single-lung ventilation could not be sustained because the tube became dislodged and could not be repositioned. Consequently, surgery was completed with periods of intermittent apnoea interspersed with manually controlled hyperventilation. Cardiovascular variables were stable during anaesthesia and no signs of hypoxia were detected. The difficulties in maintaining endobronchial tube position resulted from the animal's small size relative to the dimensions of the endotracheal tube. 相似文献
179.
Impact of correlation between pesticide parameters on estimates of environmental exposure 总被引:1,自引:0,他引:1
Monte Carlo techniques are increasingly used in pesticide exposure modelling to evaluate the uncertainty in predictions arising from uncertainty in input parameters and to estimate the confidence that should be assigned to modelling results. The approach typically involves running a deterministic model repeatedly for a large number of input values sampled from statistical distributions. A key decision in setting up a probabilistic analysis is whether there is correlation between any of the inputs to the analysis. Pesticide properties are often the most sensitive in exposure assessment. Analysis of the literature demonstrated that there are examples of both positive and negative correlation between the sorption and degradation behaviour of a pesticide, but that general trends are not apparent at present. The inclusion of even weak correlation between sorption and degradation was found to greatly influence a probabilistic analysis of leaching through soil. Correlation will reduce the predicted extent of leaching for pesticides, and it is recommended to set the correlation to zero unless the experimental data support an alternative assumption (i.e. where the correlation is statistically significant (P 相似文献
180.
Sabine WC 《Science (New York, N.Y.)》1908,27(700):841-847