Effect of transketolase-like protein 1 on proliferation of human nasopharyngeal carcinoma cells

AIM: To investigate the effect of transketolase-like protein 1 (TKTL1) on proliferation of human nasopharyngeal carcinoma cells in vitro. METHODS: The siRNA against TKTL1 mRNA was constructed and transfected into human nasopharyngeal carcinoma cells (CNE cell line). The activity of transketolase was detected before and after RNA interference.Real-time PCR was used to determine the mRNA expression of transketolase (TKT) gene family in the CNE cells.Flow cytometry and MTT test were used to detect the effect of anti-TKTL1 siRNA on cell proliferation and cell cycle in the CNE cells. RESULTS: The total transketolase activity was significantly decreased in the CNE cells transfected with siRNA TKTL1 construct compared with the cells transfected with control vector or untransfected CNE cells. No significant difference in the expression level of TKT and TKTL2 gene between the CNE cells transfected with siRNA TKTL1 construct and the cells transfected with control vector or untransfected CNE cells was observed (P>0.05). However, the expression level of TKTL1 gene was significantly downregulated in the CNE cells transfected with siRNA TKTL1 construct compared with the cells transfected with control vector.Cancer cells were arrested in G0/G1 phase, and cancer cell proliferation was significantly inhibited in the CNE cells transfected with siRNA TKTL1 construct. CONCLUSION: TKTL1 plays an important role in the total transketolase activity and cell proliferation of human nasopharyngeal carcinoma. TKTL1 may be considered as a potential target for novel anti-cancer therapy.     

Effect of angiontensin-(1-7) on development of monocrotaline induced pulmonary arterial hypertension and vascular remodeling

AIM: To investigate the effect of angiotensin-(1-7) ［Ang-(1-7)］ on the development of monocrotaline (MCT) induced pulmonary arterial hypertension and vascular remodeling.METHODS: 60 Sprgue-Dawely rats were randomly assigned into three groups: control group, PAH group and PAH+Ang-(1-7) group. Rats in PAH group and PAH +Ang-(1-7) group received 60 mg/kg MCT injection subcutaneously and after 24 h received either saline or 24 μg·kg-1·h-1 of Ang-(1-7) injection via osmotic minipumps for 4 weeks. These rats in control group were firstly injected saline subcutaneously and then received saline injection via osmotic minipumps.RESULTS: After 4 weeks, in PAH group, right ventricular systolic pressure (RVSP), right ventricular hypertrophy index (RVHI), percentage of wall thickness (WT%) and percentage of wall area (WA%) of pulmonary artery were significantly increased and NO concentration, the level of endothelial nitric oxide synthase (eNOS), eNOS Ser1177-phosphorylation were significantly decreased compared with control group. However, RVSP, RVHI, WT %, WA % were dramatically decreased in PAH +Ang-(1-7) group and NO concentration, the level of eNOS protein, eNOS Ser1177-phosphorylation were significantly increased compared with PAH group.CONCLUSION: These results suggest that Ang-(1-7) could prevent the development of monocrotaline induced pulmonary arterial hypertension and vascular remodeling, which appears to be associated with up-regulation of NO concentration and the level of eNOS protein, eNOS Ser1177-phosphorylation.     

结球甘蓝耐裂球性状的配合力及遗传力研究

按照Griffing双列杂交方法Ⅳ,选用6个结球甘蓝骨干自交系,配制15个杂交组合,进行耐裂球性状的配合力及其遗传力分析。结果表明：基因加性效应和非加性效应对耐裂球性状均起重要作用,且以加性效应为主,随着田间持续生长时间的延长其加性效应所占比例增加,GCA方差在总遗传方差中所占比例大,GCA/SCA值大于1,且差异达显著水平。选择耐裂球材料时,应着重一般配合力的选择,且应重视在早期世代进行选择。     

大白菜叶片长宽性状的QTL定位

利用一套大白菜双单倍体（DH）群体和包含287个分子标记的遗传图谱,运用复合区间作图法对叶长、无柄叶长、有柄叶长、叶宽、叶柄长、叶柄宽6个叶部形态学性状进行QTL定位及遗传效应的分析。结果表明：在4个连锁群上共检测到12个QTL,分别为叶长相关的3个,无柄叶长相关的2个,有柄叶长相关的2个,叶宽相关的1个,叶柄长相关的2个,叶柄宽相关的2个。各位点的遗传贡献率介于7.5 %~18.9 %之间。其中,叶长与叶柄长的QTL存在共定位,叶宽与叶柄宽的QTL也存在共定位。     

安徽中南部地区水生蔬菜资源与生产考察

对安徽中南部14个县、市的水生蔬菜资源进行考察,共收集水生蔬菜资源样本62份,其中野生资源18份,地方品种44 份;收集到一些有特色的地方品种如雪湖贡藕、桐城水芹、绩溪水芋、庐江芹芽等,并对安徽中南部地区水生蔬菜种植生产 情况进行了考察。根据考察结果,提出目前我国栽培的水芹应有两个种,除水芹〔Oenanthe javanica(BL.)DC.〕外,另一个 种为中华水芹(Oenanthe sinensis Dunn）。     

黑木耳液体发酵花生乳饮料的研制

试验以花生为主要原料,采用液体发酵技术研制出黑木耳液体发酵花生乳饮料。经过对影响发酵工艺条件的各因素做单因素试验后确定最佳因素值后做正交试验并确定出黑木耳液体发酵花生乳饮料的最佳方案为：花生加水比最佳值为1：10。发酵工艺的最佳条件为接种量8％,装液量70mL,发酵温度25℃,发酵时间120h。发酵后对饮料进行调配的最佳值为白糖3％,乳酸0．1％。     

黄连、黄芩提取液与二氧化氯复配对结球甘蓝种传黑斑病的抑菌效果研究

采用三元二次回归正交旋转组合设计方法,确立了黄连、黄芩提取液与二氧化氯对甘蓝链格孢菌抑制效果最佳的复配组合:黄连提取液13.48%、黄芩提取液15.56%、二氧化氯0.14%.用此复配剂浸种,芸苔链格孢菌和甘蓝链格孢菌的侵染率分别降低了97.33%和94.85%,且提高了种子发芽势及萌发相关酶活性.     

百合鳞片扦插繁殖的研究进展

鳞片繁殖是百合种球生产过程中的关键环节.综述了迄今为止有关鳞片扦插技术、影响鳞片扦插繁殖的因素和鳞片繁殖生理等方面的研究成果,并对该领域存在的问题进行了简析,对今后的研究重点进行了展望.     

几个鸢尾新品种栽培研究

对几种在郑州地区新引进的德国彩花鸢尾进行了栽培研究。结果表明:不朽白和黑骑士生长较旺,花朵较大,但开花率低,花期较短,繁殖系数低;黄娃娃和血石长势中庸,花朵中等,开花率高,花期较长,繁殖系数也高;金娃娃与黄链长势中庸偏下,花朵较小,但花期较长,金娃娃繁殖系数中等,黄链繁殖系数最低;紫边白植株矮小,长势较弱,花朵也小,但花期较长,繁殖系数较低。无论任何品种在郑州地区均能良好生长,且其生长、开花、繁殖均与土壤肥沃、疏松、排水良好呈正相关。